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The map label for this gene is ilvA [H]

Identifier: 73540119

GI number: 73540119

Start: 451846

End: 453405

Strand: Reverse

Name: ilvA [H]

Synonym: Reut_A0413

Alternate gene names: 73540119

Gene position: 453405-451846 (Counterclockwise)

Preceding gene: 73540124

Following gene: 73540118

Centisome position: 11.91

GC content: 63.21

Gene sequence:

>1560_bases
ATGAACCGCAAGCCCGCCTCCTCCAAACCCGACTATCTCAGGAAGATCCTGACCGCCAAAGTGTATGACGTGGCGCAGGA
GACCGAGCTGACCTTTGCGCACAACCTGTCGGCGCGCACCGGCAACTCGGTGTGGTTCAAGCGCGAGGACACGCAGCCGG
TGTTCTCGTTCAAGCTGCGCGGCGCATACAACAAGATGGCGTCGCTGACGCAGGAAGAACTCAGGCGCGGCGTGATCGCG
GCGTCGGCCGGCAACCACGCGCAGGGCGTGGCGCTGTCCGCGGCGCGCCTGCAGTGCCGCGCAATCATCGCCATGCCGGT
GACCACGCCGCAGGTGAAGATCGACGCGGTGCGCGAGCGCGGCGGCAAGTGGGTGGAGATCGTGCTGCACGGCGATTCGT
ACAGCGACGCCTACAACCATGCGGCAATCCTGGAGAAGAAGCACAAGCTGACCTTCATCCACCCGTTCGACGATCCGGAA
GTGATCGCCGGCCAGGGCACCATCGCAATGGAAATCCTGCGCCAGCATCCGGGCCCGATCCACGCGATCTTCGTCGCGAT
CGGTGGCGGCGGGCTGATTTCAGGCATCGCGTCTTACATCAAGGCCGTGCGCCCCGAGATCAAGGTGATCGGCGTGCAGA
CCGTGGATTCCGACGCGATGAAGCGCTCGGTCGAAGCGGGCAAGCGCATTGAACTCAAGGACGTGGGCCTGTTCTCTGAC
GGCACCGCGGTGAAGCTGGTCGGCAAGGAAACATTCCGCATCACGCGCGAACTCGTCGATGAGATCGTGCTGGTGGACAC
CGATGCGATCTGCGCGGGAGTGAAGGATGTGTTCCAGGACACGCGCAGCATTCTTGAACCCGCGGGCGCGCTGGCCGTCG
CCGGCCTCAAGCTGTATGCCGAGCGCGAGAAGCTCAAGGGCGAGCATCTGGTCGCGATCGCCTGCGGCGCCAACATGAAC
TTCGACCGCCTGCGCTTCGTCGCCGAGCGCGCCGAAGTGGGCGAGGCACGCGAAGGCGTGTTCGCCGTCACTATTCCCGA
AGAGCGCGGTAGCTTCAAGCGCTTCTGCGAACTGGTGGGCACGCGCAGCGTCACTGAATTCAACTACCGCATTGCCGACC
GGAACATCGCGCATATCTTCGTCGGCGTACAGATCGCCAGCCGCGCCGAGAACGACAAGATCGCCGCGAACTTCCGCCGC
CATGGCTTCGACACGCTGGACCTGTCCAACGACGAACTCGCCAAGCAGCACATCCGCTACATGGTTGGCGGCGTCTCGCC
GCTCGCGCACGACGAACTGCTGTATCGCTTCGAATTCCCCGAGCGCCCGGGCGCGCTGATGAAGTTCCTGTCGAGCATGA
GCCCGAACTGGAACATCAGCCTGTTCCATTACCGCAACCAGGGTGGCGACACGTCCAACATCCTGGTCGGCATCCAGGTG
CCGAAGAACGAAAAGCGCGCTTTCCGCGCATTCCTTTCGACGCTGGGTTACGTACACTGGGACGAGACCGAGAACCCGGT
CTACAAGCTGTTCCTCTCCGACCGCGGAAATCAAGCATGA

Upstream 100 bases:

>100_bases
TGGCGGTTTCCGCCAAAATTTCCGGCCTTTTTTGCGCCCTTTTTGCCCTTCCCCGCGTCGTTCTGCGTCGCGCCGCCAGG
GCCCATCGCCAACCGCAGAC

Downstream 100 bases:

>100_bases
GCCTTCCTGAACACTCGCCGCTGGGCAAGCCCTCGGCCTACAAGACGGAATACGACGCGACGCTGCTGTTCCCGATCGCG
CGCCAGCCCAAGCGCACGGA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNRKPASSKPDYLRKILTAKVYDVAQETELTFAHNLSARTGNSVWFKREDTQPVFSFKLRGAYNKMASLTQEELRRGVIA
ASAGNHAQGVALSAARLQCRAIIAMPVTTPQVKIDAVRERGGKWVEIVLHGDSYSDAYNHAAILEKKHKLTFIHPFDDPE
VIAGQGTIAMEILRQHPGPIHAIFVAIGGGGLISGIASYIKAVRPEIKVIGVQTVDSDAMKRSVEAGKRIELKDVGLFSD
GTAVKLVGKETFRITRELVDEIVLVDTDAICAGVKDVFQDTRSILEPAGALAVAGLKLYAEREKLKGEHLVAIACGANMN
FDRLRFVAERAEVGEAREGVFAVTIPEERGSFKRFCELVGTRSVTEFNYRIADRNIAHIFVGVQIASRAENDKIAANFRR
HGFDTLDLSNDELAKQHIRYMVGGVSPLAHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGGDTSNILVGIQV
PKNEKRAFRAFLSTLGYVHWDETENPVYKLFLSDRGNQA

Sequences:

>Translated_519_residues
MNRKPASSKPDYLRKILTAKVYDVAQETELTFAHNLSARTGNSVWFKREDTQPVFSFKLRGAYNKMASLTQEELRRGVIA
ASAGNHAQGVALSAARLQCRAIIAMPVTTPQVKIDAVRERGGKWVEIVLHGDSYSDAYNHAAILEKKHKLTFIHPFDDPE
VIAGQGTIAMEILRQHPGPIHAIFVAIGGGGLISGIASYIKAVRPEIKVIGVQTVDSDAMKRSVEAGKRIELKDVGLFSD
GTAVKLVGKETFRITRELVDEIVLVDTDAICAGVKDVFQDTRSILEPAGALAVAGLKLYAEREKLKGEHLVAIACGANMN
FDRLRFVAERAEVGEAREGVFAVTIPEERGSFKRFCELVGTRSVTEFNYRIADRNIAHIFVGVQIASRAENDKIAANFRR
HGFDTLDLSNDELAKQHIRYMVGGVSPLAHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGGDTSNILVGIQV
PKNEKRAFRAFLSTLGYVHWDETENPVYKLFLSDRGNQA
>Mature_519_residues
MNRKPASSKPDYLRKILTAKVYDVAQETELTFAHNLSARTGNSVWFKREDTQPVFSFKLRGAYNKMASLTQEELRRGVIA
ASAGNHAQGVALSAARLQCRAIIAMPVTTPQVKIDAVRERGGKWVEIVLHGDSYSDAYNHAAILEKKHKLTFIHPFDDPE
VIAGQGTIAMEILRQHPGPIHAIFVAIGGGGLISGIASYIKAVRPEIKVIGVQTVDSDAMKRSVEAGKRIELKDVGLFSD
GTAVKLVGKETFRITRELVDEIVLVDTDAICAGVKDVFQDTRSILEPAGALAVAGLKLYAEREKLKGEHLVAIACGANMN
FDRLRFVAERAEVGEAREGVFAVTIPEERGSFKRFCELVGTRSVTEFNYRIADRNIAHIFVGVQIASRAENDKIAANFRR
HGFDTLDLSNDELAKQHIRYMVGGVSPLAHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGGDTSNILVGIQV
PKNEKRAFRAFLSTLGYVHWDETENPVYKLFLSDRGNQA

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=265, Percent_Identity=34.3396226415094, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI33469958, Length=313, Percent_Identity=29.073482428115, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1790207, Length=516, Percent_Identity=50.1937984496124, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789505, Length=308, Percent_Identity=38.961038961039, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71991565, Length=305, Percent_Identity=31.8032786885246, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=300, Percent_Identity=31, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17537387, Length=321, Percent_Identity=30.2180685358255, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320930, Length=518, Percent_Identity=48.2625482625483, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322631, Length=304, Percent_Identity=33.2236842105263, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6319788, Length=302, Percent_Identity=25.1655629139073, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355833, Length=319, Percent_Identity=31.0344827586207, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645328, Length=255, Percent_Identity=29.0196078431373, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 57569; Mature: 57569

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKPASSKPDYLRKILTAKVYDVAQETELTFAHNLSARTGNSVWFKREDTQPVFSFKLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEC
GAYNKMASLTQEELRRGVIAASAGNHAQGVALSAARLQCRAIIAMPVTTPQVKIDAVRER
CHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEHHHHEEEEEEEEECCCCCCEEEHHHHHC
GGKWVEIVLHGDSYSDAYNHAAILEKKHKLTFIHPFDDPEVIAGQGTIAMEILRQHPGPI
CCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCEEECCCHHHHHHHHHCCCCE
HAIFVAIGGGGLISGIASYIKAVRPEIKVIGVQTVDSDAMKRSVEAGKRIELKDVGLFSD
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCEEEEEECCEECC
GTAVKLVGKETFRITRELVDEIVLVDTDAICAGVKDVFQDTRSILEPAGALAVAGLKLYA
CCEEEEECHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EREKLKGEHLVAIACGANMNFDRLRFVAERAEVGEAREGVFAVTIPEERGSFKRFCELVG
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHCCEEEEEECHHCCCHHHHHHHHC
TRSVTEFNYRIADRNIAHIFVGVQIASRAENDKIAANFRRHGFDTLDLSNDELAKQHIRY
CCCEEEEEEEECCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHH
MVGGVSPLAHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGGDTSNILVGIQV
HHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEC
PKNEKRAFRAFLSTLGYVHWDETENPVYKLFLSDRGNQA
CCCHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MNRKPASSKPDYLRKILTAKVYDVAQETELTFAHNLSARTGNSVWFKREDTQPVFSFKLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEC
GAYNKMASLTQEELRRGVIAASAGNHAQGVALSAARLQCRAIIAMPVTTPQVKIDAVRER
CHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEHHHHEEEEEEEEECCCCCCEEEHHHHHC
GGKWVEIVLHGDSYSDAYNHAAILEKKHKLTFIHPFDDPEVIAGQGTIAMEILRQHPGPI
CCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCEEECCCHHHHHHHHHCCCCE
HAIFVAIGGGGLISGIASYIKAVRPEIKVIGVQTVDSDAMKRSVEAGKRIELKDVGLFSD
EEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCEEEEEECCEECC
GTAVKLVGKETFRITRELVDEIVLVDTDAICAGVKDVFQDTRSILEPAGALAVAGLKLYA
CCEEEEECHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EREKLKGEHLVAIACGANMNFDRLRFVAERAEVGEAREGVFAVTIPEERGSFKRFCELVG
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHCCEEEEEECHHCCCHHHHHHHHC
TRSVTEFNYRIADRNIAHIFVGVQIASRAENDKIAANFRRHGFDTLDLSNDELAKQHIRY
CCCEEEEEEEECCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHH
MVGGVSPLAHDELLYRFEFPERPGALMKFLSSMSPNWNISLFHYRNQGGDTSNILVGIQV
HHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEC
PKNEKRAFRAFLSTLGYVHWDETENPVYKLFLSDRGNQA
CCCHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA