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The map label for this gene is queF [H]

Identifier: 73540118

GI number: 73540118

Start: 451016

End: 451849

Strand: Reverse

Name: queF [H]

Synonym: Reut_A0412

Alternate gene names: 73540118

Gene position: 451849-451016 (Counterclockwise)

Preceding gene: 73540119

Following gene: 73540116

Centisome position: 11.87

GC content: 59.59

Gene sequence:

>834_bases
ATGAGCCTTCCTGAACACTCGCCGCTGGGCAAGCCCTCGGCCTACAAGACGGAATACGACGCGACGCTGCTGTTCCCGAT
CGCGCGCCAGCCCAAGCGCACGGAAATCGGCCTGCCCGCAGGCAAGCCCGTGCCGTTCTTCGGCGTAGATATCTGGAATG
CGTATGAAGTGTCGTGGCTCAACATGCGCGGCAAGCCGCAGGTCGCGCTGGCCACGTTCATCATCCCGTCCGATACGCCG
AACATCATCGAATCGAAGTCGTTCAAGCTGTACCTGAACTCATTCAACCAGACAAAAATCGCCTCGCCTGAAGCGCTGCA
ACAACTGCTCCACCATGATCTCTCGGAAGCCACCGGCGGCACCGTGCAGGTGCGGCTGGTGACGGAAGCCGATCTGGGCA
AGCAGAAGATGGGCGAGCTGGAGGGATTGCTGCTCGATCGGCTGGACATCGAGGTCGATCGCTACGAGCCGGCACCCGAC
TTGCTGAAGGCTGACCAGCAGGAATCACCGGTCGAGGAAACGCTGGTGTCGCACCTGCTGAAGTCCAATTGCCTGGTGAC
CGGGCAGCCCGATTGGGGCAGTGTGCAGATTCGCTATGTGGGCGCGCCGATCAACCAGGAAGGGCTGCTGAAATACCTGA
TCTCGTTCCGCAATCACAATGAATTCCATGAGCAGTGCGTGGAGCGGATCTTTATGGACGTGATGAGGGAGTGCAAGCCG
GTGAAGCTTGCCGTCTATGCGCGCTACACGCGACGCGGCGGGCTGGATATCAATCCGTTCCGCACCAACTTCAATACGCC
CTGGCCGGATAATCTCAGGAACGCAAGGCAGTAA

Upstream 100 bases:

>100_bases
GCGCTTTCCGCGCATTCCTTTCGACGCTGGGTTACGTACACTGGGACGAGACCGAGAACCCGGTCTACAAGCTGTTCCTC
TCCGACCGCGGAAATCAAGC

Downstream 100 bases:

>100_bases
GGGAATCTGAGGTGCCGGATGGTGTTGGCGTGTGTGGCTCGCGCCAACACCCCTCAAAACTCGGCGTGAAACCGGATCCC
GTAGAACTTCGCCGGCCCGC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSLPEHSPLGKPSAYKTEYDATLLFPIARQPKRTEIGLPAGKPVPFFGVDIWNAYEVSWLNMRGKPQVALATFIIPSDTP
NIIESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGGTVQVRLVTEADLGKQKMGELEGLLLDRLDIEVDRYEPAPD
LLKADQQESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFRNHNEFHEQCVERIFMDVMRECKP
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ

Sequences:

>Translated_277_residues
MSLPEHSPLGKPSAYKTEYDATLLFPIARQPKRTEIGLPAGKPVPFFGVDIWNAYEVSWLNMRGKPQVALATFIIPSDTP
NIIESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGGTVQVRLVTEADLGKQKMGELEGLLLDRLDIEVDRYEPAPD
LLKADQQESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFRNHNEFHEQCVERIFMDVMRECKP
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ
>Mature_276_residues
SLPEHSPLGKPSAYKTEYDATLLFPIARQPKRTEIGLPAGKPVPFFGVDIWNAYEVSWLNMRGKPQVALATFIIPSDTPN
IIESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGGTVQVRLVTEADLGKQKMGELEGLLLDRLDIEVDRYEPAPDL
LKADQQESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFRNHNEFHEQCVERIFMDVMRECKPV
KLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=53.7037037037037, Blast_Score=281, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 31418; Mature: 31287

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPEHSPLGKPSAYKTEYDATLLFPIARQPKRTEIGLPAGKPVPFFGVDIWNAYEVSWL
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEE
NMRGKPQVALATFIIPSDTPNIIESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGG
CCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
TVQVRLVTEADLGKQKMGELEGLLLDRLDIEVDRYEPAPDLLKADQQESPVEETLVSHLL
EEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCCCHHHHHCCCCCCCHHHHHHHHHH
KSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFRNHNEFHEQCVERIFMDVMRECKP
HCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ
EEEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SLPEHSPLGKPSAYKTEYDATLLFPIARQPKRTEIGLPAGKPVPFFGVDIWNAYEVSWL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEE
NMRGKPQVALATFIIPSDTPNIIESKSFKLYLNSFNQTKIASPEALQQLLHHDLSEATGG
CCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
TVQVRLVTEADLGKQKMGELEGLLLDRLDIEVDRYEPAPDLLKADQQESPVEETLVSHLL
EEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCCCHHHHHCCCCCCCHHHHHHHHHH
KSNCLVTGQPDWGSVQIRYVGAPINQEGLLKYLISFRNHNEFHEQCVERIFMDVMRECKP
HCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
VKLAVYARYTRRGGLDINPFRTNFNTPWPDNLRNARQ
EEEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA