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The map label for this gene is aceA [C]

Identifier: 73540090

GI number: 73540090

Start: 420269

End: 421855

Strand: Reverse

Name: aceA [C]

Synonym: Reut_A0384

Alternate gene names: 73540090

Gene position: 421855-420269 (Counterclockwise)

Preceding gene: 73540093

Following gene: 73540086

Centisome position: 11.08

GC content: 62.26

Gene sequence:

>1587_bases
ATGGCTCAGTATCAAGACGACATCAAGGCAGTTGCTGGTTTGAAGGAGAAACACGGCAGCGCGTGGGACGCCATCAATCC
CGAGTACGCCGCTCGCATGCGGGCCCAGAACCGGTTCAAGACGGGCCTGGATATCGCCAAGTACACTGCCAAGATCATGC
GCGCCGACATGGCCGCCTATGATGCCGATCCGTCGAAGTACACCCAGTCGCTCGGCTGCTGGCACGGCTTCATCGGCCAG
CAGAAGATGATCTCCATCAAGAAGCACTTCAACAACAACACCGACCGCCGCTACCTGTATCTGTCGGGCTGGATGGTCGC
CGCACTGCGCTCGGAGTTCGGCCCGCTGCCGGACCAGTCGATGCACGAGAAGACCTCGGTCAGCGCCCTGATCCGCGAGC
TGTACACCTTCCTGCGCCAGGCCGACGCCCGTGAACTGGGCGGCCTGTTCCGCGAACTGGACGCCGCCAAGGACGCCACC
GCCAAGGCTGCCATCCAGTCGAAGATCGACAACCACGTCACCCACGTGGTGCCCATCATCGCTGACATCGACGCGGGCTT
CGGCAACGCCGAGGCCACCTACCTGCTGGCCAAACAGTTCATCGAAGCCGGCGCCTGCTGCATCCAGATCGAAAACCAGG
TGTCCGACGAGAAGCAGTGCGGCCACCAGGACGGCAAGGTCACCGTGCCGCACGAAGACTTCCTGGCCAAGGTTCGCGCA
GTCCGCTACGCCTTCCTGGAACTGGGCGTGGACGACGGCATCATCGTGACGCGCACCGACTCGCTGGGCGCCGGCCTGAC
CAAGCAGATCGCCGTGACCCACACGCCGGGCGACCTGGGCGACCAGTACAACTCGTTCCTCGACTGCGACGAAGTCTCGG
CCGACACGCTGGGCAACGGTGACATCGTCATCAAGCAGAACGGCAAGCTGATGCGCCCGAAGCGCCTGCCGAGCAACCTG
TTCCAGTTCCGCGCCGGTACGGGTGAGGCACGCTGCGTGCTGGATTGCATCACCTCGCTGCAGAACGGCGCCGATCTGCT
GTGGATCGAAACCGAAAAGCCGCATATCGCCCAGATCGCCGGCATGGTCAACGAGATCCGCAAGGTCATCCCGAACGCCA
AGCTGGTGTACAACAACAGCCCGTCGTTCAACTGGACCCTGAACTTCCGTCAGCAAGCGTATGACGCCATGAAGGCAGCA
GGCAAGGATGTATCGGCCTACGAGCGTGCCCAGCTGATGAGCGTGGAATACGACGACACCGAACTGGCCAAGATCGCCGA
CGAGAAGATCCGCACCTTCCAGGCCGACGCATCGCGTGAAGCCGGTATCTTCCACCACCTGATCACGCTGCCGACGTACC
ACACCGCCGCGCTGTCGACCGACAACCTGGCCAAGGAATACTTCGGCGACCAGGGCATGCTCGGTTATGTGGCTGGCGTG
CAGCGCAAGGAAATCCGTCAGGGCATCGCCTGCGTCAAGCACCAGAACATGTCGGGCTCGGACATCGGCGACGACCACAA
GGAATACTTCAGCGGCGAAGCGGCCCTGAAGGCAGCGGGTAAAGACAACACGATGAACCAGTTCTGA

Upstream 100 bases:

>100_bases
ATCAAATGGCGCTACTATTTACCCATGCAGTTCGCTGCCACATACGCAGCGGCAAGCTCCGATCCTCTACTCAAACGCGC
TTTGCTCCCAGGAGAGACAA

Downstream 100 bases:

>100_bases
TATCGGTTCATCGCCCAAAGCAAAACGCCAACCCACAAGGGTTGGCGTTTTTTTTGCGAGACACCGGAGCAGTGAGCCGG
TTGTCGCCTCCTGGTCTAGG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAQYQDDIKAVAGLKEKHGSAWDAINPEYAARMRAQNRFKTGLDIAKYTAKIMRADMAAYDADPSKYTQSLGCWHGFIGQ
QKMISIKKHFNNNTDRRYLYLSGWMVAALRSEFGPLPDQSMHEKTSVSALIRELYTFLRQADARELGGLFRELDAAKDAT
AKAAIQSKIDNHVTHVVPIIADIDAGFGNAEATYLLAKQFIEAGACCIQIENQVSDEKQCGHQDGKVTVPHEDFLAKVRA
VRYAFLELGVDDGIIVTRTDSLGAGLTKQIAVTHTPGDLGDQYNSFLDCDEVSADTLGNGDIVIKQNGKLMRPKRLPSNL
FQFRAGTGEARCVLDCITSLQNGADLLWIETEKPHIAQIAGMVNEIRKVIPNAKLVYNNSPSFNWTLNFRQQAYDAMKAA
GKDVSAYERAQLMSVEYDDTELAKIADEKIRTFQADASREAGIFHHLITLPTYHTAALSTDNLAKEYFGDQGMLGYVAGV
QRKEIRQGIACVKHQNMSGSDIGDDHKEYFSGEAALKAAGKDNTMNQF

Sequences:

>Translated_528_residues
MAQYQDDIKAVAGLKEKHGSAWDAINPEYAARMRAQNRFKTGLDIAKYTAKIMRADMAAYDADPSKYTQSLGCWHGFIGQ
QKMISIKKHFNNNTDRRYLYLSGWMVAALRSEFGPLPDQSMHEKTSVSALIRELYTFLRQADARELGGLFRELDAAKDAT
AKAAIQSKIDNHVTHVVPIIADIDAGFGNAEATYLLAKQFIEAGACCIQIENQVSDEKQCGHQDGKVTVPHEDFLAKVRA
VRYAFLELGVDDGIIVTRTDSLGAGLTKQIAVTHTPGDLGDQYNSFLDCDEVSADTLGNGDIVIKQNGKLMRPKRLPSNL
FQFRAGTGEARCVLDCITSLQNGADLLWIETEKPHIAQIAGMVNEIRKVIPNAKLVYNNSPSFNWTLNFRQQAYDAMKAA
GKDVSAYERAQLMSVEYDDTELAKIADEKIRTFQADASREAGIFHHLITLPTYHTAALSTDNLAKEYFGDQGMLGYVAGV
QRKEIRQGIACVKHQNMSGSDIGDDHKEYFSGEAALKAAGKDNTMNQF
>Mature_527_residues
AQYQDDIKAVAGLKEKHGSAWDAINPEYAARMRAQNRFKTGLDIAKYTAKIMRADMAAYDADPSKYTQSLGCWHGFIGQQ
KMISIKKHFNNNTDRRYLYLSGWMVAALRSEFGPLPDQSMHEKTSVSALIRELYTFLRQADARELGGLFRELDAAKDATA
KAAIQSKIDNHVTHVVPIIADIDAGFGNAEATYLLAKQFIEAGACCIQIENQVSDEKQCGHQDGKVTVPHEDFLAKVRAV
RYAFLELGVDDGIIVTRTDSLGAGLTKQIAVTHTPGDLGDQYNSFLDCDEVSADTLGNGDIVIKQNGKLMRPKRLPSNLF
QFRAGTGEARCVLDCITSLQNGADLLWIETEKPHIAQIAGMVNEIRKVIPNAKLVYNNSPSFNWTLNFRQQAYDAMKAAG
KDVSAYERAQLMSVEYDDTELAKIADEKIRTFQADASREAGIFHHLITLPTYHTAALSTDNLAKEYFGDQGMLGYVAGVQ
RKEIRQGIACVKHQNMSGSDIGDDHKEYFSGEAALKAAGKDNTMNQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=410, Percent_Identity=27.3170731707317, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982926, Length=410, Percent_Identity=27.3170731707317, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17561814, Length=410, Percent_Identity=27.3170731707317, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320908, Length=172, Percent_Identity=30.8139534883721, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 58444; Mature: 58312

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYQDDIKAVAGLKEKHGSAWDAINPEYAARMRAQNRFKTGLDIAKYTAKIMRADMAAY
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DADPSKYTQSLGCWHGFIGQQKMISIKKHFNNNTDRRYLYLSGWMVAALRSEFGPLPDQS
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCCCCCH
MHEKTSVSALIRELYTFLRQADARELGGLFRELDAAKDATAKAAIQSKIDNHVTHVVPII
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADIDAGFGNAEATYLLAKQFIEAGACCIQIENQVSDEKQCGHQDGKVTVPHEDFLAKVRA
HCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCEEECCHHHHHHHHHH
VRYAFLELGVDDGIIVTRTDSLGAGLTKQIAVTHTPGDLGDQYNSFLDCDEVSADTLGNG
HHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCC
DIVIKQNGKLMRPKRLPSNLFQFRAGTGEARCVLDCITSLQNGADLLWIETEKPHIAQIA
CEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GMVNEIRKVIPNAKLVYNNSPSFNWTLNFRQQAYDAMKAAGKDVSAYERAQLMSVEYDDT
HHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCH
ELAKIADEKIRTFQADASREAGIFHHLITLPTYHTAALSTDNLAKEYFGDQGMLGYVAGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHCCCCCCCHHHHCC
QRKEIRQGIACVKHQNMSGSDIGDDHKEYFSGEAALKAAGKDNTMNQF
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHEECCCCCCCCCC
>Mature Secondary Structure 
AQYQDDIKAVAGLKEKHGSAWDAINPEYAARMRAQNRFKTGLDIAKYTAKIMRADMAAY
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DADPSKYTQSLGCWHGFIGQQKMISIKKHFNNNTDRRYLYLSGWMVAALRSEFGPLPDQS
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCCCCCH
MHEKTSVSALIRELYTFLRQADARELGGLFRELDAAKDATAKAAIQSKIDNHVTHVVPII
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADIDAGFGNAEATYLLAKQFIEAGACCIQIENQVSDEKQCGHQDGKVTVPHEDFLAKVRA
HCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCEEECCHHHHHHHHHH
VRYAFLELGVDDGIIVTRTDSLGAGLTKQIAVTHTPGDLGDQYNSFLDCDEVSADTLGNG
HHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCC
DIVIKQNGKLMRPKRLPSNLFQFRAGTGEARCVLDCITSLQNGADLLWIETEKPHIAQIA
CEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GMVNEIRKVIPNAKLVYNNSPSFNWTLNFRQQAYDAMKAAGKDVSAYERAQLMSVEYDDT
HHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCH
ELAKIADEKIRTFQADASREAGIFHHLITLPTYHTAALSTDNLAKEYFGDQGMLGYVAGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHCCCCCCCHHHHCC
QRKEIRQGIACVKHQNMSGSDIGDDHKEYFSGEAALKAAGKDNTMNQF
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]