Click here to switch to the map view.

The map label for this gene is pth

Identifier: 73540046

GI number: 73540046

Start: 369436

End: 370035

Strand: Reverse

Name: pth

Synonym: Reut_A0340

Alternate gene names: 73540046

Gene position: 370035-369436 (Counterclockwise)

Preceding gene: 73540047

Following gene: 73540040

Centisome position: 9.72

GC content: 67.67

Gene sequence:

>600_bases
ATGATCAAGCTCATCGTCGGTCTCGGCAATCCCGGAGCGGAATACGAGGCCACCCGCCACAACGCCGGCTTCTGGCTCGT
GGACCAGCTTGCCCGCATGGGCGGCACCACGCTGCGGGTGGAAGGCCGTTTCCACGGCCTGGCCGGGCGCGCGCGCCTGT
GGGACCAGGACATCTGGCTGCTCAAGCCCTCCACGTTCATGAACCGGTCCGGCTTGGCCGTGGTATCGCTGGCCCGCTTC
TACAAGATTCTGCCCGACGAAATCGTCGTCGCGCATGACGAAATGGATCTGCCCCCGGGCGCCGCCAAGCTCAAGCGCGG
CGGCGGCGCGGGCGGCCACAATGGCCTGAAGGACATTGCCGCCCACCTGACCACGCAGGAGTTCTGGCGTCTGCGCCTGG
GCGTCGGTCATCCGCGCAACCTGCCCGGTGGTGCCGGCGCCGGGCGCGAGGATGTCGTCAACTTCGTGCTCAAGCCACCG
CGCAAGGAAGAACAGCAGGCGATCGACGAAGCGATCGACCGTAGCATCGATCCGCTCGGCCTGCTGGCCCGCGGCGATGC
GGAACGCGCCATGGCGCAACTTCACACTGTGGCGCGCTGA

Upstream 100 bases:

>100_bases
AAGACGAAAAAACCGCCGGTCATGCCGGCGGTTTTTTTTGGCGTATGCTTTGCGCCTGCGGACGACAGCAACGCTGCCGC
CAACCAACCCTGTTCATCGC

Downstream 100 bases:

>100_bases
CCCCGGCGCACATCGTCGCACGGCCGTTGCATCGCCGCCAAAAACAAAGCCCCCGTTACCTCTCGGTGACGGGGGCTTTG
TTTTTGTACCCTGCCGATTG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVEGRFHGLAGRARLWDQDIWLLKPSTFMNRSGLAVVSLARF
YKILPDEIVVAHDEMDLPPGAAKLKRGGGAGGHNGLKDIAAHLTTQEFWRLRLGVGHPRNLPGGAGAGREDVVNFVLKPP
RKEEQQAIDEAIDRSIDPLGLLARGDAERAMAQLHTVAR

Sequences:

>Translated_199_residues
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVEGRFHGLAGRARLWDQDIWLLKPSTFMNRSGLAVVSLARF
YKILPDEIVVAHDEMDLPPGAAKLKRGGGAGGHNGLKDIAAHLTTQEFWRLRLGVGHPRNLPGGAGAGREDVVNFVLKPP
RKEEQQAIDEAIDRSIDPLGLLARGDAERAMAQLHTVAR
>Mature_199_residues
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVEGRFHGLAGRARLWDQDIWLLKPSTFMNRSGLAVVSLARF
YKILPDEIVVAHDEMDLPPGAAKLKRGGGAGGHNGLKDIAAHLTTQEFWRLRLGVGHPRNLPGGAGAGREDVVNFVLKPP
RKEEQQAIDEAIDRSIDPLGLLARGDAERAMAQLHTVAR

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=176, Percent_Identity=34.6590909090909, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787455, Length=175, Percent_Identity=56.5714285714286, Blast_Score=164, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_CUPPJ (Q476G1)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294566.1
- ProteinModelPortal:   Q476G1
- SMR:   Q476G1
- GeneID:   3609580
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0340
- NMPDR:   fig|264198.3.peg.852
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   REUT264198:REUT_A0340-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21692; Mature: 21692

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVEGRFHGLAGRARLWDQDIWL
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCEEE
LKPSTFMNRSGLAVVSLARFYKILPDEIVVAHDEMDLPPGAAKLKRGGGAGGHNGLKDIA
ECCHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHH
AHLTTQEFWRLRLGVGHPRNLPGGAGAGREDVVNFVLKPPRKEEQQAIDEAIDRSIDPLG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
LLARGDAERAMAQLHTVAR
EEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKLIVGLGNPGAEYEATRHNAGFWLVDQLARMGGTTLRVEGRFHGLAGRARLWDQDIWL
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCCEEE
LKPSTFMNRSGLAVVSLARFYKILPDEIVVAHDEMDLPPGAAKLKRGGGAGGHNGLKDIA
ECCHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCHHHHH
AHLTTQEFWRLRLGVGHPRNLPGGAGAGREDVVNFVLKPPRKEEQQAIDEAIDRSIDPLG
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
LLARGDAERAMAQLHTVAR
EEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA