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The map label for this gene is rsmD [H]

Identifier: 73540043

GI number: 73540043

Start: 367691

End: 368335

Strand: Direct

Name: rsmD [H]

Synonym: Reut_A0337

Alternate gene names: 73540043

Gene position: 367691-368335 (Clockwise)

Preceding gene: 73540042

Following gene: 73540044

Centisome position: 9.66

GC content: 68.53

Gene sequence:

>645_bases
ATGAATTCGCGTACCCGATCTCCATCGCCCGCCGCGAGCGGGCGATCGTCTTCTCCGGCGCCGCGCCAGGCGCCGTCCCA
GGTCCGTATCATCGGTGGCCGCTGGAAGCGCACGTTGCTGCCCGTCCCCGACGCGCAAGGCTTGCGGCCGACGCCCGACC
GCGTGCGGGAAACCCTGTTTAACTGGCTGGGCCAGGACTTGGCCGGGTTGTATTGCCTGGACCTGTTTGCGGGCTCGGGC
GCACTCGGTTTCGAAGCGGCCTCCCGCGGTGCCGAAGCCGTCACGCTGGTGGAATTCAACCCGCGTGTCGCCAAGCAGTT
GCGCGACAACCAATACCGGCTCGATGCCGCGCACATTCGCGTCGTGCAGGGTGATGCCTTCGCGATTGCCGCGCAGATGC
CGGATGCCAGCTTCGACGTGATTTTTTTGGATCCGCCGTTCGCCGAAGACTGGCTGCGGCCCGCCCTTGAGCACGCCGCG
CGGCTGCTGCGCCCTGGCGGCGCGGTGTACGTGGAGACGGATCGTTCCCTGACTGGTGCCGATGCACCGGTGCCTGCGTC
GCTGGAGATCGTCCGCCAGGCGCGGGCCGGTGCGGTGCACTTCCATCTCCTGCAGCACCGCGGCGCCGAGGGGGGCGCTG
CGTAA

Upstream 100 bases:

>100_bases
TCGTCGGCGGCCCGGAGTAAGCATGCGCCTTGTGGGGCTTGCCGCACGCAGCGCAATGGCCGGTGATGGCTGAAGCGCCG
ACAGGCTCTACAATCACGGC

Downstream 100 bases:

>100_bases
CGCCGGTAGCCGCACACGGTACGGTGCAGAGCACAGATCGACGCCTTCCAATTCACGGATTTAGGGTTACACTACGCCGC
TGTTACAACGCTCGCGACGC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNSRTRSPSPAASGRSSSPAPRQAPSQVRIIGGRWKRTLLPVPDAQGLRPTPDRVRETLFNWLGQDLAGLYCLDLFAGSG
ALGFEAASRGAEAVTLVEFNPRVAKQLRDNQYRLDAAHIRVVQGDAFAIAAQMPDASFDVIFLDPPFAEDWLRPALEHAA
RLLRPGGAVYVETDRSLTGADAPVPASLEIVRQARAGAVHFHLLQHRGAEGGAA

Sequences:

>Translated_214_residues
MNSRTRSPSPAASGRSSSPAPRQAPSQVRIIGGRWKRTLLPVPDAQGLRPTPDRVRETLFNWLGQDLAGLYCLDLFAGSG
ALGFEAASRGAEAVTLVEFNPRVAKQLRDNQYRLDAAHIRVVQGDAFAIAAQMPDASFDVIFLDPPFAEDWLRPALEHAA
RLLRPGGAVYVETDRSLTGADAPVPASLEIVRQARAGAVHFHLLQHRGAEGGAA
>Mature_214_residues
MNSRTRSPSPAASGRSSSPAPRQAPSQVRIIGGRWKRTLLPVPDAQGLRPTPDRVRETLFNWLGQDLAGLYCLDLFAGSG
ALGFEAASRGAEAVTLVEFNPRVAKQLRDNQYRLDAAHIRVVQGDAFAIAAQMPDASFDVIFLDPPFAEDWLRPALEHAA
RLLRPGGAVYVETDRSLTGADAPVPASLEIVRQARAGAVHFHLLQHRGAEGGAA

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=191, Percent_Identity=44.5026178010471, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23021; Mature: 23021

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRTRSPSPAASGRSSSPAPRQAPSQVRIIGGRWKRTLLPVPDAQGLRPTPDRVRETLF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHH
NWLGQDLAGLYCLDLFAGSGALGFEAASRGAEAVTLVEFNPRVAKQLRDNQYRLDAAHIR
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEECHHHHHHHHCCCEEEEHEEEE
VVQGDAFAIAAQMPDASFDVIFLDPPFAEDWLRPALEHAARLLRPGGAVYVETDRSLTGA
EEECCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
DAPVPASLEIVRQARAGAVHFHLLQHRGAEGGAA
CCCCCHHHHHHHHHHCCHHEEHHHHHCCCCCCCC
>Mature Secondary Structure
MNSRTRSPSPAASGRSSSPAPRQAPSQVRIIGGRWKRTLLPVPDAQGLRPTPDRVRETLF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHH
NWLGQDLAGLYCLDLFAGSGALGFEAASRGAEAVTLVEFNPRVAKQLRDNQYRLDAAHIR
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEECHHHHHHHHCCCEEEEHEEEE
VVQGDAFAIAAQMPDASFDVIFLDPPFAEDWLRPALEHAARLLRPGGAVYVETDRSLTGA
EEECCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
DAPVPASLEIVRQARAGAVHFHLLQHRGAEGGAA
CCCCCHHHHHHHHHHCCHHEEHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]