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The map label for this gene is mhpC

Identifier: 73539980

GI number: 73539980

Start: 305039

End: 305908

Strand: Direct

Name: mhpC

Synonym: Reut_A0274

Alternate gene names: 73539980

Gene position: 305039-305908 (Clockwise)

Preceding gene: 73539979

Following gene: 73539984

Centisome position: 8.01

GC content: 61.95

Gene sequence:

>870_bases
ATGCAAGCGAACGTACAAGCCATCACCGAAGCCGGCACAAGCCGTTTCGTGACCGTGAAGGATGGCGATACCGAATTCCG
CATCCACTGCAACGACACCGGCGCCGGTGCCGAGACTGTCGTCATGCTGCACGGCTCGGGTCCCGGCGCGACCGGCTGGG
CCAACTTCAATCGCAATGTCGAGCCGCTGGTCGCGGCCGGTTATCGCGTGCTGCTGGTCGATTGCCCGGGCTGGGGCAAG
AGCGATCCTGTCGTCAACGCGGGCTCGCGTTCGGAACTCAATGGCCGCGTGCTCAAGGGTGTGCTGGACGAACTTGATAT
CGAACGCGTGCACATCCTTGGCAACTCGATGGGCGGTCACAGCGCGGTGGCATTTGCGCTTGCCAACCCGCAGCGTGTGG
GCAAGCTCGTGCTGATGGGTGGTGGCACCGGCGGCCCGAGCCTGTACGCGCCGATGCCGACGGAAGGCATCAAGCTGCTC
AACGGCCTGTACCGCGAGCCGTCGATCGAAAACCTGAAGCGCATGATGAATGTGTTCGTCTATGACGCCAGTTCACTGAC
TGACGATCTGATGCAGGCGCGGCTCGACAATATGCTGGCGCGGCGCGACCACCTCGAGAATTTCGTGAAAAGCCTGGCGG
CGAATCCCAAGCAGTTCACCGACTACGGTTCGCGCCTGGGCGAGATCACGGCGCCGACGCTAGTGATCTGGGGGCGTGAC
GATCGCTTCGTGCCGATGGATGTCGGGCTGCGGCTGATCGCCGGGATTCCGAATGCGCAGATGCATATCTTCAATCGCTG
CGGGCACTGGGCGCAGTGGGAGCATGCGAAGGCGTTTAATCGGATGGTCGTGGATTTTTTGGGGAATTGA

Upstream 100 bases:

>100_bases
TGGCATCCGGCGCGGCAGTTCCAGGCGGGCGCGGCGCGTGAGCGGCGCACTGTCAGCACATTCCATTCCCTACAGATTCA
GTCGAGCAAGGAAGATCAAC

Downstream 100 bases:

>100_bases
GGGGGAGCTTGGGGCAGGGTGTCGTTCTTGGCGCGAGCGAAGAGCAATGTCCCAGGTGCAGCTGAGACCTCAAAAAAACG
CCCCGCAGAAACGCGGGGCA

Product: Alpha/beta hydrolase fold

Products: NA

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MQANVQAITEAGTSRFVTVKDGDTEFRIHCNDTGAGAETVVMLHGSGPGATGWANFNRNVEPLVAAGYRVLLVDCPGWGK
SDPVVNAGSRSELNGRVLKGVLDELDIERVHILGNSMGGHSAVAFALANPQRVGKLVLMGGGTGGPSLYAPMPTEGIKLL
NGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHLENFVKSLAANPKQFTDYGSRLGEITAPTLVIWGRD
DRFVPMDVGLRLIAGIPNAQMHIFNRCGHWAQWEHAKAFNRMVVDFLGN

Sequences:

>Translated_289_residues
MQANVQAITEAGTSRFVTVKDGDTEFRIHCNDTGAGAETVVMLHGSGPGATGWANFNRNVEPLVAAGYRVLLVDCPGWGK
SDPVVNAGSRSELNGRVLKGVLDELDIERVHILGNSMGGHSAVAFALANPQRVGKLVLMGGGTGGPSLYAPMPTEGIKLL
NGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHLENFVKSLAANPKQFTDYGSRLGEITAPTLVIWGRD
DRFVPMDVGLRLIAGIPNAQMHIFNRCGHWAQWEHAKAFNRMVVDFLGN
>Mature_289_residues
MQANVQAITEAGTSRFVTVKDGDTEFRIHCNDTGAGAETVVMLHGSGPGATGWANFNRNVEPLVAAGYRVLLVDCPGWGK
SDPVVNAGSRSELNGRVLKGVLDELDIERVHILGNSMGGHSAVAFALANPQRVGKLVLMGGGTGGPSLYAPMPTEGIKLL
NGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHLENFVKSLAANPKQFTDYGSRLGEITAPTLVIWGRD
DRFVPMDVGLRLIAGIPNAQMHIFNRCGHWAQWEHAKAFNRMVVDFLGN

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family

Homologues:

Organism=Escherichia coli, GI87081721, Length=285, Percent_Identity=69.8245614035088, Blast_Score=436, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MHPC_CUPPJ (Q476M7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294500.1
- ProteinModelPortal:   Q476M7
- SMR:   Q476M7
- GeneID:   3610013
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0274
- NMPDR:   fig|264198.3.peg.786
- HOGENOM:   HBG748003
- OMA:   MLARRDH
- ProtClustDB:   CLSK879654
- BioCyc:   REUT264198:REUT_A0274-MONOMER
- HAMAP:   MF_01654
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: 3.7.1.-

Molecular weight: Translated: 31460; Mature: 31460

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: ACT_SITE 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANVQAITEAGTSRFVTVKDGDTEFRIHCNDTGAGAETVVMLHGSGPGATGWANFNRNV
CCCCHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
EPLVAAGYRVLLVDCPGWGKSDPVVNAGSRSELNGRVLKGVLDELDIERVHILGNSMGGH
CHHHHCCEEEEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
SAVAFALANPQRVGKLVLMGGGTGGPSLYAPMPTEGIKLLNGLYREPSIENLKRMMNVFV
CEEEEEECCHHHCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHE
YDASSLTDDLMQARLDNMLARRDHLENFVKSLAANPKQFTDYGSRLGEITAPTLVIWGRD
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCC
DRFVPMDVGLRLIAGIPNAQMHIFNRCGHWAQWEHAKAFNRMVVDFLGN
CCEEEHHHCHHEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQANVQAITEAGTSRFVTVKDGDTEFRIHCNDTGAGAETVVMLHGSGPGATGWANFNRNV
CCCCHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
EPLVAAGYRVLLVDCPGWGKSDPVVNAGSRSELNGRVLKGVLDELDIERVHILGNSMGGH
CHHHHCCEEEEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
SAVAFALANPQRVGKLVLMGGGTGGPSLYAPMPTEGIKLLNGLYREPSIENLKRMMNVFV
CEEEEEECCHHHCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHE
YDASSLTDDLMQARLDNMLARRDHLENFVKSLAANPKQFTDYGSRLGEITAPTLVIWGRD
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCC
DRFVPMDVGLRLIAGIPNAQMHIFNRCGHWAQWEHAKAFNRMVVDFLGN
CCEEEHHHCHHEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA