BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

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The map label for this gene is braF [H]

Identifier: 73539967

GI number: 73539967

Start: 290741

End: 291661

Strand: Direct

Name: braF [H]

Synonym: Reut_A0261

Alternate gene names: 73539967

Gene position: 290741-291661 (Clockwise)

Preceding gene: 73539966

Following gene: 73539968

Centisome position: 7.64

GC content: 65.04

Gene sequence:

>921_bases
ATGCCCGCGGCGCGTACGGACGCGAGCGGTGCAATGCCTGGTGGCGGCGCCATCGCGCCGGTTGGCCCGATGGCGCCCGA
TACGCGGGCTGATCTCGGCCTGCAGACCGGCACGCGGCATTCGCAGCAGACGCATGAGCACGCGCGCACCGGCGGCGAGG
TGATCCTCGACCTGCAGAACATCTCGCTGTCGTTCGGCGGCGTGAAGGCGCTGACGGACATCTCGTTCAATGTGTGCGAG
CACGAGATCCGCGCGATCATCGGCCCCAATGGCGCGGGCAAGAGTTCGATGCTGAACGTGATCAACGGCGTGTATCACCC
GCAGCAGGGACGCATCGTGTTCCGTGGCGAGGAACGCCGCCAGATGCATCCGACCGCCGCTGCGCGCCAGGGCATCGCGC
GCACGTTCCAGAACATCGCGCTGTTCAAGGGCATGACCGTGCTCGACAACATCATGACCGGCCGCAACACGCAGTTCCGC
ACGAGCTTCCTCGCCCATGCGCTGTGGTGGGGCCCGGCGCGCAACGAGGAAATGCGGCACCGTCAGAAGGTGGAAGAGGT
GATCGACTTCCTGGAAATCCAGTCGATCCGCAAGACGCCGGTCGGGCGCCTGCCGTACGGCTTGCAGAAGCGCGTGGAGC
TGGCACGCGCACTGGCGGCCGAGCCGTCGATGCTGCTGCTCGACGAGCCGATGGCCGGCATGAACGTGGAAGAGAAGCAG
GACATGTGCCGCTTCATCCTGGACGTGAACCGCCAGTTCGGCACCACCATCGTGCTGATCGAGCACGACATGGGCGTGGT
GATGGATATCTCGGACCGCGTGGTGGTACTCGACTACGGCAAGAAGATCGGTGACGGGACGCCGGAAGAGGTGAAGGCGA
ATCCAGATGTGATCAAGGCCTACCTCGGTACGTCGCACTGA

Upstream 100 bases:

>100_bases
CTGAGGCTGATGGATGCCAAACGCCTGCCGCCGGCGGCACGCGCGGCATGAGCAGGGGAGCGAAAGCATGACGCAAGTGG
CCTGGCAAGGAACAGACCGC

Downstream 100 bases:

>100_bases
GCCCGCACGCGGCTTTCCATTCAACTGATTCCACCGGAACGCAGCCATGACGTTCTTCATTGAAATCCTGCTCGGCGGCT
TGCTGTCGGGCCTGATGTAC

Product: ABC transporter related

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPAARTDASGAMPGGGAIAPVGPMAPDTRADLGLQTGTRHSQQTHEHARTGGEVILDLQNISLSFGGVKALTDISFNVCE
HEIRAIIGPNGAGKSSMLNVINGVYHPQQGRIVFRGEERRQMHPTAAARQGIARTFQNIALFKGMTVLDNIMTGRNTQFR
TSFLAHALWWGPARNEEMRHRQKVEEVIDFLEIQSIRKTPVGRLPYGLQKRVELARALAAEPSMLLLDEPMAGMNVEEKQ
DMCRFILDVNRQFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPEEVKANPDVIKAYLGTSH

Sequences:

>Translated_306_residues
MPAARTDASGAMPGGGAIAPVGPMAPDTRADLGLQTGTRHSQQTHEHARTGGEVILDLQNISLSFGGVKALTDISFNVCE
HEIRAIIGPNGAGKSSMLNVINGVYHPQQGRIVFRGEERRQMHPTAAARQGIARTFQNIALFKGMTVLDNIMTGRNTQFR
TSFLAHALWWGPARNEEMRHRQKVEEVIDFLEIQSIRKTPVGRLPYGLQKRVELARALAAEPSMLLLDEPMAGMNVEEKQ
DMCRFILDVNRQFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPEEVKANPDVIKAYLGTSH
>Mature_305_residues
PAARTDASGAMPGGGAIAPVGPMAPDTRADLGLQTGTRHSQQTHEHARTGGEVILDLQNISLSFGGVKALTDISFNVCEH
EIRAIIGPNGAGKSSMLNVINGVYHPQQGRIVFRGEERRQMHPTAAARQGIARTFQNIALFKGMTVLDNIMTGRNTQFRT
SFLAHALWWGPARNEEMRHRQKVEEVIDFLEIQSIRKTPVGRLPYGLQKRVELARALAAEPSMLLLDEPMAGMNVEEKQD
MCRFILDVNRQFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPEEVKANPDVIKAYLGTSH

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI47078218, Length=227, Percent_Identity=25.1101321585903, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI45446740, Length=227, Percent_Identity=25.1101321585903, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI27262624, Length=168, Percent_Identity=30.952380952381, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27262626, Length=168, Percent_Identity=30.952380952381, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI153792144, Length=170, Percent_Identity=30, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI21536378, Length=230, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI31657092, Length=253, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI27881501, Length=243, Percent_Identity=25.5144032921811, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI30795238, Length=243, Percent_Identity=25.5144032921811, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI27436953, Length=217, Percent_Identity=24.4239631336406, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI21536376, Length=255, Percent_Identity=25.0980392156863, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI105990541, Length=227, Percent_Identity=22.9074889867841, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI11967969, Length=229, Percent_Identity=24.4541484716157, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=35.0597609561753, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1788761, Length=240, Percent_Identity=30.4166666666667, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081791, Length=242, Percent_Identity=30.1652892561983, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788450, Length=254, Percent_Identity=29.9212598425197, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789991, Length=232, Percent_Identity=33.6206896551724, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI48995001, Length=233, Percent_Identity=30.0429184549356, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790467, Length=240, Percent_Identity=30.4166666666667, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788210, Length=248, Percent_Identity=30.241935483871, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787712, Length=253, Percent_Identity=26.0869565217391, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787029, Length=253, Percent_Identity=28.0632411067194, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790190, Length=233, Percent_Identity=30.4721030042918, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786398, Length=247, Percent_Identity=29.1497975708502, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789593, Length=248, Percent_Identity=28.6290322580645, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081709, Length=241, Percent_Identity=26.5560165975104, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788472, Length=235, Percent_Identity=29.3617021276596, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790525, Length=248, Percent_Identity=27.8225806451613, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789032, Length=270, Percent_Identity=29.2592592592593, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789672, Length=249, Percent_Identity=28.5140562248996, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787370, Length=241, Percent_Identity=28.2157676348548, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081782, Length=251, Percent_Identity=28.2868525896414, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790739, Length=236, Percent_Identity=29.2372881355932, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI87082267, Length=252, Percent_Identity=26.1904761904762, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082268, Length=251, Percent_Identity=29.0836653386454, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788165, Length=247, Percent_Identity=26.7206477732794, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789586, Length=246, Percent_Identity=29.2682926829268, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786345, Length=258, Percent_Identity=26.3565891472868, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786563, Length=226, Percent_Identity=28.3185840707965, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786872, Length=258, Percent_Identity=27.5193798449612, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI48994943, Length=241, Percent_Identity=26.9709543568465, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786253, Length=232, Percent_Identity=28.448275862069, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789962, Length=234, Percent_Identity=24.7863247863248, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787500, Length=267, Percent_Identity=25.8426966292135, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786803, Length=233, Percent_Identity=24.4635193133047, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786319, Length=210, Percent_Identity=29.0476190476191, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994997, Length=241, Percent_Identity=24.4813278008299, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788897, Length=249, Percent_Identity=25.7028112449799, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787759, Length=261, Percent_Identity=28.3524904214559, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI145693107, Length=253, Percent_Identity=26.0869565217391, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786698, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787758, Length=260, Percent_Identity=23.8461538461538, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790544, Length=250, Percent_Identity=25.6, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789891, Length=215, Percent_Identity=29.3023255813954, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789873, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788644, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=27.9661016949153, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787792, Length=246, Percent_Identity=25.2032520325203, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787547, Length=262, Percent_Identity=24.0458015267176, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081834, Length=233, Percent_Identity=24.8927038626609, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994883, Length=263, Percent_Identity=25.8555133079848, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787548, Length=255, Percent_Identity=25.8823529411765, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788053, Length=229, Percent_Identity=26.2008733624454, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786981, Length=213, Percent_Identity=28.169014084507, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789963, Length=250, Percent_Identity=24.8, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787143, Length=250, Percent_Identity=25.2, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786703, Length=236, Percent_Identity=24.1525423728814, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788506, Length=273, Percent_Identity=25.2747252747253, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787112, Length=253, Percent_Identity=24.901185770751, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787041, Length=241, Percent_Identity=24.0663900414938, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787182, Length=247, Percent_Identity=23.4817813765182, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534520, Length=243, Percent_Identity=27.5720164609054, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193202349, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71984940, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115533592, Length=259, Percent_Identity=23.9382239382239, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17533971, Length=231, Percent_Identity=23.3766233766234, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=227, Percent_Identity=23.7885462555066, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17569145, Length=273, Percent_Identity=25.2747252747253, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221512771, Length=257, Percent_Identity=26.8482490272374, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648314, Length=255, Percent_Identity=24.3137254901961, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24666092, Length=230, Percent_Identity=25.2173913043478, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571209, Length=277, Percent_Identity=22.7436823104693, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573571, Length=240, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643674, Length=245, Percent_Identity=22.8571428571429, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116007184, Length=241, Percent_Identity=21.1618257261411, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221500365, Length=241, Percent_Identity=21.1618257261411, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573573, Length=231, Percent_Identity=26.8398268398268, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI23397593, Length=247, Percent_Identity=27.1255060728745, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 33630; Mature: 33499

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAARTDASGAMPGGGAIAPVGPMAPDTRADLGLQTGTRHSQQTHEHARTGGEVILDLQN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCEEEEEEEC
ISLSFGGVKALTDISFNVCEHEIRAIIGPNGAGKSSMLNVINGVYHPQQGRIVFRGEERR
CEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHH
QMHPTAAARQGIARTFQNIALFKGMTVLDNIMTGRNTQFRTSFLAHALWWGPARNEEMRH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHH
RQKVEEVIDFLEIQSIRKTPVGRLPYGLQKRVELARALAAEPSMLLLDEPMAGMNVEEKQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
DMCRFILDVNRQFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPEEVKANPDVIKA
HHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCCHHHHCCCHHHHHH
YLGTSH
HHCCCC
>Mature Secondary Structure 
PAARTDASGAMPGGGAIAPVGPMAPDTRADLGLQTGTRHSQQTHEHARTGGEVILDLQN
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCEEEEEEEC
ISLSFGGVKALTDISFNVCEHEIRAIIGPNGAGKSSMLNVINGVYHPQQGRIVFRGEERR
CEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHH
QMHPTAAARQGIARTFQNIALFKGMTVLDNIMTGRNTQFRTSFLAHALWWGPARNEEMRH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHH
RQKVEEVIDFLEIQSIRKTPVGRLPYGLQKRVELARALAAEPSMLLLDEPMAGMNVEEKQ
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
DMCRFILDVNRQFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPEEVKANPDVIKA
HHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCCCHHHHCCCHHHHHH
YLGTSH
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]