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The map label for this gene is emrB [H]

Identifier: 73539945

GI number: 73539945

Start: 263400

End: 265010

Strand: Direct

Name: emrB [H]

Synonym: Reut_A0239

Alternate gene names: 73539945

Gene position: 263400-265010 (Clockwise)

Preceding gene: 73539944

Following gene: 73539948

Centisome position: 6.92

GC content: 65.11

Gene sequence:

>1611_bases
ATGAGCACGGCCACACTCGACAGGCCGGTCGCGGCACCGGCGGCGGCGTTGCCGTCCCCGGCATCGCTATCGATGCCCGC
GAAGATCTTCGCCTTCGCGACCATGTGCGTGGGCATGTTCATCGCGCTGCTCGATATCCAGATCGTCTCGGCGTCGCTGC
GCGATATTGGTGGCGGCCTGTCGGCGGGCGCCGACGAGACGGTCTGGGTCCAGACCAGCTACCTGATCGCGGAGATCATC
GTCATTCCGCTGTCGGGCTGGTTGTCTCGCGTGATGTCGACGCGCTGGCTGTTTGCCGCGTCGGCCGCGGGGTTCACGCT
AACCAGCCTGCTGTGCGGCGTGGCGTGGAATATTCAGAGCATGATCGCGTTCCGTGCGGCGCAGGGCTTCCTGGGCGGGT
CGATGATCCCGCTGGTGTTCACCACGGCCTTTGCGTTCTTTGCCGGCCCGCAGCGCGTGATCGCCGCAGCGACCATCGGC
GCGCTCGCGTCTCTCGCGCCAACGCTTGGCCCGACCATCGGCGGCTGGATTACCGACAACTTCTCGTGGCACTGGCTGTT
CTTCATCAACCTGGTGCCCGGCATCTTCGTCACGGTGGCCGTGCCGATGCTGGTCAAGGTCGACCGCCCGAACTGGTCGC
TGGTGCGCAGCGCCGACTACCTCGGCATTGTGCTCATGGCGCTGTTTCTGGGTTGCCTCGAATACACGATGGAAGAGGGG
CCGCGCTGGGACTGGTTTGGCGACGAAGTGATCCTCGCCACCGCATGGATTGCCGGGCTATCGGGCGTGGCCTTTGTCTG
GCGATCGCTGACCTACGCGAATCCCGTGGTGGACCTGCGCGCGCTGCGCGAGCGCAACTTCGCATTGGGCTGCTTTTTCT
CGTTCGTCACCGGTATCGGCATCTTCGCGACGATCTACCTGACACCGTTGTTCCTGGGACGCGTGCGCGGCTTCAGCGCG
CTGGACATCGGCCTGGCGATCTTCTCGACGGGCCTGTTTCAGGTCGCATCGATTCCGCTCTATGCGTTCCTGGCCAATCG
CGTCGACCTGCGCTGGCTGATGATGTTCGGACTCGGCCTGTTCGCGCTGTCGATGTGGTTCTTCGCACCGATCACTCACG
ACTGGGGCGGCGCCGAACTGCTGCTGCCGCAGGCACTGCGCGGCATGGCACAGCAGTTCGCGGTGGCGCCGACGGTCACG
CTGACGCTGGGCAGCCTGCCGCCCGAACGGCTCAAGCTCGCATCAGGCCTGTTCAACCTGATGCGCAACCTGGGCGGCGC
CATCGGCATCGCGGCCTGCGCCACCGTGCTCAACGACCGCGGCAACCTGCATTTCCTGCGCGAGGCGGAGCACCTGAATA
TCCGCAACGAGGCCATGGGCGCGTTCCTGCAAAGCGCAGGAAGCCAGATGCTCGCGGCTGGCCACGACCTGGTCGATGCG
AATACGGTCGCGTTGAAGCAACTCTTTGCGCTCGCGTGGCGTGAAGCGCAGACCCAGACGTTCGGCGATGCGTTCCTGAT
CGTCATGGTGTGTTTCGTGATTGCCACCGCGATGGTCCCGCTGATGCGCAAGGTCGCGCCGCCGAAGGCTCCGTCTGCGG
ATGCGCATTGA

Upstream 100 bases:

>100_bases
ACGAGAAAGATGCCACGCTCGGCACCTTGCGCCCGGGCCTGTCCGTGATCGCGCAGGTCGATACGCATCCGGATACGCAT
TCAACCAGGCAGGTGCAATG

Downstream 100 bases:

>100_bases
GCCGCAGTGCTACGGAGTTCAGCCATGAAACTTGATGCCCTGACGCGTTTGCTCTGTACTGTGATGTTGGCCACATGCGC
CACTACCGCGGCGCGCGCCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSTATLDRPVAAPAAALPSPASLSMPAKIFAFATMCVGMFIALLDIQIVSASLRDIGGGLSAGADETVWVQTSYLIAEII
VIPLSGWLSRVMSTRWLFAASAAGFTLTSLLCGVAWNIQSMIAFRAAQGFLGGSMIPLVFTTAFAFFAGPQRVIAAATIG
ALASLAPTLGPTIGGWITDNFSWHWLFFINLVPGIFVTVAVPMLVKVDRPNWSLVRSADYLGIVLMALFLGCLEYTMEEG
PRWDWFGDEVILATAWIAGLSGVAFVWRSLTYANPVVDLRALRERNFALGCFFSFVTGIGIFATIYLTPLFLGRVRGFSA
LDIGLAIFSTGLFQVASIPLYAFLANRVDLRWLMMFGLGLFALSMWFFAPITHDWGGAELLLPQALRGMAQQFAVAPTVT
LTLGSLPPERLKLASGLFNLMRNLGGAIGIAACATVLNDRGNLHFLREAEHLNIRNEAMGAFLQSAGSQMLAAGHDLVDA
NTVALKQLFALAWREAQTQTFGDAFLIVMVCFVIATAMVPLMRKVAPPKAPSADAH

Sequences:

>Translated_536_residues
MSTATLDRPVAAPAAALPSPASLSMPAKIFAFATMCVGMFIALLDIQIVSASLRDIGGGLSAGADETVWVQTSYLIAEII
VIPLSGWLSRVMSTRWLFAASAAGFTLTSLLCGVAWNIQSMIAFRAAQGFLGGSMIPLVFTTAFAFFAGPQRVIAAATIG
ALASLAPTLGPTIGGWITDNFSWHWLFFINLVPGIFVTVAVPMLVKVDRPNWSLVRSADYLGIVLMALFLGCLEYTMEEG
PRWDWFGDEVILATAWIAGLSGVAFVWRSLTYANPVVDLRALRERNFALGCFFSFVTGIGIFATIYLTPLFLGRVRGFSA
LDIGLAIFSTGLFQVASIPLYAFLANRVDLRWLMMFGLGLFALSMWFFAPITHDWGGAELLLPQALRGMAQQFAVAPTVT
LTLGSLPPERLKLASGLFNLMRNLGGAIGIAACATVLNDRGNLHFLREAEHLNIRNEAMGAFLQSAGSQMLAAGHDLVDA
NTVALKQLFALAWREAQTQTFGDAFLIVMVCFVIATAMVPLMRKVAPPKAPSADAH
>Mature_535_residues
STATLDRPVAAPAAALPSPASLSMPAKIFAFATMCVGMFIALLDIQIVSASLRDIGGGLSAGADETVWVQTSYLIAEIIV
IPLSGWLSRVMSTRWLFAASAAGFTLTSLLCGVAWNIQSMIAFRAAQGFLGGSMIPLVFTTAFAFFAGPQRVIAAATIGA
LASLAPTLGPTIGGWITDNFSWHWLFFINLVPGIFVTVAVPMLVKVDRPNWSLVRSADYLGIVLMALFLGCLEYTMEEGP
RWDWFGDEVILATAWIAGLSGVAFVWRSLTYANPVVDLRALRERNFALGCFFSFVTGIGIFATIYLTPLFLGRVRGFSAL
DIGLAIFSTGLFQVASIPLYAFLANRVDLRWLMMFGLGLFALSMWFFAPITHDWGGAELLLPQALRGMAQQFAVAPTVTL
TLGSLPPERLKLASGLFNLMRNLGGAIGIAACATVLNDRGNLHFLREAEHLNIRNEAMGAFLQSAGSQMLAAGHDLVDAN
TVALKQLFALAWREAQTQTFGDAFLIVMVCFVIATAMVPLMRKVAPPKAPSADAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=448, Percent_Identity=31.9196428571429, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788710, Length=494, Percent_Identity=28.5425101214575, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1790195, Length=432, Percent_Identity=24.3055555555556, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081983, Length=430, Percent_Identity=26.5116279069767, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=26.2773722627737, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57651; Mature: 57520

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATLDRPVAAPAAALPSPASLSMPAKIFAFATMCVGMFIALLDIQIVSASLRDIGGGL
CCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAGADETVWVQTSYLIAEIIVIPLSGWLSRVMSTRWLFAASAAGFTLTSLLCGVAWNIQS
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MIAFRAAQGFLGGSMIPLVFTTAFAFFAGPQRVIAAATIGALASLAPTLGPTIGGWITDN
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
FSWHWLFFINLVPGIFVTVAVPMLVKVDRPNWSLVRSADYLGIVLMALFLGCLEYTMEEG
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCC
PRWDWFGDEVILATAWIAGLSGVAFVWRSLTYANPVVDLRALRERNFALGCFFSFVTGIG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHH
IFATIYLTPLFLGRVRGFSALDIGLAIFSTGLFQVASIPLYAFLANRVDLRWLMMFGLGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FALSMWFFAPITHDWGGAELLLPQALRGMAQQFAVAPTVTLTLGSLPPERLKLASGLFNL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHH
MRNLGGAIGIAACATVLNDRGNLHFLREAEHLNIRNEAMGAFLQSAGSQMLAAGHDLVDA
HHHHCCHHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHCCCHHHHCCCCCCCC
NTVALKQLFALAWREAQTQTFGDAFLIVMVCFVIATAMVPLMRKVAPPKAPSADAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
STATLDRPVAAPAAALPSPASLSMPAKIFAFATMCVGMFIALLDIQIVSASLRDIGGGL
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]