Click here to switch to the map view.

The map label for this gene is emrA [H]

Identifier: 73539944

GI number: 73539944

Start: 262273

End: 263400

Strand: Direct

Name: emrA [H]

Synonym: Reut_A0238

Alternate gene names: 73539944

Gene position: 262273-263400 (Clockwise)

Preceding gene: 73539943

Following gene: 73539945

Centisome position: 6.89

GC content: 67.29

Gene sequence:

>1128_bases
ATGACAAGCAACACCATCACCGAACGCACGGGCGAGCAGCCTGTTCCGGCCGCGAAACAGGCAGGATCGCGGCCCGCTAC
GAAGAAGCTGCTCGCGACCGGGGCGCTGCTTGTCGCGGGTCTGGCCGGTGTCGGCTACGGCTACCACTGGTGGAGCTCCG
CGCGCTTCGTGCAGAGCACCGACGATGCCTACGTGGGCGGCGACGTGACCGTGATCGGCGCGAAGGTTGCCGGCTATATC
TCCGAAGTTGCCGTGACCGACAACCAGCCTGTGCATGCAGGGGATGTGCTGGTGCGCATCGACGACCGCGACTATCGTGC
CGCGCTGGCCAAGGCCGAGGGCGCGGTGGCTGCGCAGCAGGCTCTGCTCGAAAACCTCGACGCCACGCAGCGGCTGCAGG
AAGCCGTCATCGGCCAGGCGCGGGCCGGCTTCGCGGGCGCCAATGCCGACACCGTTCGTGCGCGCCAGGACCAGGCACGC
TATGCGAGCCTGGTCGAAAAATCGGCGGTGTCGATCCAGAGTTCGCAGAAGGCCGATGCCGACTACAAGCAGGCGGCCGC
CAACAGCGAAAAGGCGCAAGCGTCGCTGCTCGCCGCGCAGCGCCAGCTCGAGGTGATCGGCACGCAGAAGCTGCAGGCGA
AAGCGGCGCTGGCGCAGGCCGTGGCGGAACGCGACATCGCAAAGCTCAACCTCAGCTACACCGAACTGCGCGCGCCGGTC
GACGGCACGGTGGGCAACCGCCGCGCGCGCGTCGGCGCCTACGCCGGCAGCGGTAGTCAGTTGCTGTCCATCGTTCCGGC
CAGCGGCTTGTGGGTCGATGCAAACTTCAAGGAAGGTCAGCTCGCGCATATGCGCGAGGGCATGCCTGTCACCATCGAGG
CCGATGTGCTGCCCGGACGCGTCTTCCATGGCCGCGTCGGCAGCCTCGCGCCGGCGACTGGGGCGCAGTTCAGCGTGCTG
CCGGCCGAAAACGCGACCGGTAACTTCACCAAGATCGTCCAGCGCGTGCCGGTACGCATCCAGCTCGACGAGAAAGATGC
CACGCTCGGCACCTTGCGCCCGGGCCTGTCCGTGATCGCGCAGGTCGATACGCATCCGGATACGCATTCAACCAGGCAGG
TGCAATGA

Upstream 100 bases:

>100_bases
CCGAACGCATGCGGCAGAAATTGAATCGTGCAGCGGCGATGCAGGCCGCATCCGGCTCGAATCTGAATCCCATCGAAACT
GAGTGCTGAAAGGAAGTGCC

Downstream 100 bases:

>100_bases
TGAGCACGGCCACACTCGACAGGCCGGTCGCGGCACCGGCGGCGGCGTTGCCGTCCCCGGCATCGCTATCGATGCCCGCG
AAGATCTTCGCCTTCGCGAC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTSNTITERTGEQPVPAAKQAGSRPATKKLLATGALLVAGLAGVGYGYHWWSSARFVQSTDDAYVGGDVTVIGAKVAGYI
SEVAVTDNQPVHAGDVLVRIDDRDYRAALAKAEGAVAAQQALLENLDATQRLQEAVIGQARAGFAGANADTVRARQDQAR
YASLVEKSAVSIQSSQKADADYKQAAANSEKAQASLLAAQRQLEVIGTQKLQAKAALAQAVAERDIAKLNLSYTELRAPV
DGTVGNRRARVGAYAGSGSQLLSIVPASGLWVDANFKEGQLAHMREGMPVTIEADVLPGRVFHGRVGSLAPATGAQFSVL
PAENATGNFTKIVQRVPVRIQLDEKDATLGTLRPGLSVIAQVDTHPDTHSTRQVQ

Sequences:

>Translated_375_residues
MTSNTITERTGEQPVPAAKQAGSRPATKKLLATGALLVAGLAGVGYGYHWWSSARFVQSTDDAYVGGDVTVIGAKVAGYI
SEVAVTDNQPVHAGDVLVRIDDRDYRAALAKAEGAVAAQQALLENLDATQRLQEAVIGQARAGFAGANADTVRARQDQAR
YASLVEKSAVSIQSSQKADADYKQAAANSEKAQASLLAAQRQLEVIGTQKLQAKAALAQAVAERDIAKLNLSYTELRAPV
DGTVGNRRARVGAYAGSGSQLLSIVPASGLWVDANFKEGQLAHMREGMPVTIEADVLPGRVFHGRVGSLAPATGAQFSVL
PAENATGNFTKIVQRVPVRIQLDEKDATLGTLRPGLSVIAQVDTHPDTHSTRQVQ
>Mature_374_residues
TSNTITERTGEQPVPAAKQAGSRPATKKLLATGALLVAGLAGVGYGYHWWSSARFVQSTDDAYVGGDVTVIGAKVAGYIS
EVAVTDNQPVHAGDVLVRIDDRDYRAALAKAEGAVAAQQALLENLDATQRLQEAVIGQARAGFAGANADTVRARQDQARY
ASLVEKSAVSIQSSQKADADYKQAAANSEKAQASLLAAQRQLEVIGTQKLQAKAALAQAVAERDIAKLNLSYTELRAPVD
GTVGNRRARVGAYAGSGSQLLSIVPASGLWVDANFKEGQLAHMREGMPVTIEADVLPGRVFHGRVGSLAPATGAQFSVLP
AENATGNFTKIVQRVPVRIQLDEKDATLGTLRPGLSVIAQVDTHPDTHSTRQVQ

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=368, Percent_Identity=35.8695652173913, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1788711, Length=353, Percent_Identity=33.4277620396601, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790519, Length=320, Percent_Identity=34.375, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081951, Length=324, Percent_Identity=28.3950617283951, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789637, Length=335, Percent_Identity=26.865671641791, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787013, Length=324, Percent_Identity=28.0864197530864, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790024, Length=300, Percent_Identity=24.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790012, Length=248, Percent_Identity=29.4354838709677, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39454; Mature: 39323

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNTITERTGEQPVPAAKQAGSRPATKKLLATGALLVAGLAGVGYGYHWWSSARFVQST
CCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEECC
DDAYVGGDVTVIGAKVAGYISEVAVTDNQPVHAGDVLVRIDDRDYRAALAKAEGAVAAQQ
CCCEECCCEEEEHHHHHHHHHHEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHH
ALLENLDATQRLQEAVIGQARAGFAGANADTVRARQDQARYASLVEKSAVSIQSSQKADA
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DYKQAAANSEKAQASLLAAQRQLEVIGTQKLQAKAALAQAVAERDIAKLNLSYTELRAPV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
DGTVGNRRARVGAYAGSGSQLLSIVPASGLWVDANFKEGQLAHMREGMPVTIEADVLPGR
CCCCCCCCEEEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHCCCCEEEEECCCCCE
VFHGRVGSLAPATGAQFSVLPAENATGNFTKIVQRVPVRIQLDEKDATLGTLRPGLSVIA
EECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCHHHCCCCCEEEE
QVDTHPDTHSTRQVQ
EECCCCCCCCCCCCC
>Mature Secondary Structure 
TSNTITERTGEQPVPAAKQAGSRPATKKLLATGALLVAGLAGVGYGYHWWSSARFVQST
CCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEECC
DDAYVGGDVTVIGAKVAGYISEVAVTDNQPVHAGDVLVRIDDRDYRAALAKAEGAVAAQQ
CCCEECCCEEEEHHHHHHHHHHEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHH
ALLENLDATQRLQEAVIGQARAGFAGANADTVRARQDQARYASLVEKSAVSIQSSQKADA
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DYKQAAANSEKAQASLLAAQRQLEVIGTQKLQAKAALAQAVAERDIAKLNLSYTELRAPV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
DGTVGNRRARVGAYAGSGSQLLSIVPASGLWVDANFKEGQLAHMREGMPVTIEADVLPGR
CCCCCCCCEEEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHCCCCEEEEECCCCCE
VFHGRVGSLAPATGAQFSVLPAENATGNFTKIVQRVPVRIQLDEKDATLGTLRPGLSVIA
EECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCCCCCHHHCCCCCEEEE
QVDTHPDTHSTRQVQ
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]