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The map label for this gene is thiC [H]

Identifier: 73539911

GI number: 73539911

Start: 232007

End: 233887

Strand: Direct

Name: thiC [H]

Synonym: Reut_A0205

Alternate gene names: 73539911

Gene position: 232007-233887 (Clockwise)

Preceding gene: 73539909

Following gene: 73539912

Centisome position: 6.09

GC content: 64.54

Gene sequence:

>1881_bases
ATGGCCCGTACTGCCCCCGCCGCATCATTCGAATCGCTCGAGAGCGACCTCGACCAGAAGTTTGCCTACCCGGCTTCCAG
CAAGACCTACATCCCCGGCAGCCGCCCGGATATCCGCGTGCCGATGCGCACGATCCTGCAGACGTCCACGCGTACCGAGA
AGGGCGAAATGCCCAATCCGCCGATCCCCGTGTACGACACGTCGGGCCCGTACAGCGACCCGGACGTGCATATCGACCTG
AAGGCCGGCCTGCCGGCGCTGCGCGAAAAGTGGATCGCAGAACGCGGCGACACCGAAGTGCTGCCCGGCCTGTCGTCGGA
ATATGGCCGTGACCGCGCCAACGACCCGGCCACCGCGCACCTGCGCTTTGCCCAGCTGACGAACCCGCGCCGTGCGAAGG
CCGGCGCCAACGTGTCGCAGATGCACTATGCGCGCAAGGGCATCATCACGCCGGAAATGGAATACGTGGCGCTGCGCGAA
TCGCTGAACCTGCAGGCGCTGTACGACAAGCCCGAATACAAGGCGCTGCTGCGCCAGCACCCGGGCAATGCGCTGGGCGC
CGGCCTGCCGCTGCGTCCGGAAGACATCACGCCGGAATTCGTGCGCAACGAGATTGCCACGGGTCGCGCGATCATCCCCG
CCAACATCAACCACACCGAGCTGGAGCCGATGGCGATCGGGCGCAATTTCCGCGTCAAGATCAACGGCAACCTGGGCAAC
TCCGCCGTGACGTCGTCGCTGGCCGAGGAAGTGGAAAAGATGGTGTGGTCGATCCGCTGGGGCGCCGACACCATCATGGA
CCTGTCGACCGGCAAGCACATCCATGAAACGCGCGAATGGATCCTGCGCAATTCGCCGGTGCCTATCGGCACGGTGCCGA
TCTACCAGGCGCTCGACAAGACCGGCGGCATCGCCGAGGACCTGACCTGGGAGATGTTCCGCGACACGCTGATCGAGCAG
GCCGAGCAGGGCGTGGACTACTTCACGATCCATGCCGGCGTGCTGCTGCGCTACGTGCCGCTGACGGCCGACCGCGTGAC
GGGCATTGTCTCGCGCGGCGGTTCGATCATGGCCAAGTGGTGCCTGGCGCATCACAAGGAGAACTTCCTGTACACGCACT
TCGACGAGATCTGCGAGATCATGAAGGCGTACGACGTGTCGTTCAGCCTCGGCGACGGCCTGCGTCCGGGTTGCATCGCG
GATTCGAACGATGACGCGCAATTCGGCGAACTGCGCACGCTTGGCGAGCTGACAGCCAAGGCATGGAAGCACGACGTGCA
GGTCATGATCGAAGGTCCGGGCCACGTGCCGCTGCAGCGCATCCAGGCGAACATGGATGAAGAGCTCAAGCACTGCTACG
AGGCACCGTTCTACACGCTCGGACCGCTCGTGACCGACATCGCCCCGGGCTACGACCACATCACCAGCGGCATCGGCGCG
GCCAACATCGGCTGGATGGGCACGGCCATGCTGTGCTACGTCACGCCGAAGGAGCACCTGGGCCTGCCGGACAAGGAAGA
CGTGCGCGAGGGCATCATCACCTACAAGATCGCCGCCCACGCCGCCGATCTGGCCAAGGGCTGGCCCGGCGCGCAGCTGC
GCGACAACGCGCTGTCGAAGGCGCGCTTCGAGTTCCGCTGGGAAGACCAGTTCAACCTCGGCCTGGATCCGGAACGCGCA
CGTTCGTACCACGACGCCACGCTGCCGGCCGAAGGCGCCAAGATTGCCCACTTCTGTTCGATGTGCGGGCCGAAGTTCTG
CTCGATGAAGATCACGCAGGAAGTGCGCGACTACGCGGCATCGCTGCCGAAGGAAGCGCAGCAGGGCATGGAAGAGAAGT
CGATCGAGTTCCTGAAGAAGGGCAGCAAGATCTATTCGTAA

Upstream 100 bases:

>100_bases
GTCTCTTGTCCCTCCGGGACTCGCCTCCCCGGCCTCAGGCCCCGGCGGCGCTCACAGGGCTCCTGGTTTCGTCCACCCCG
CAAGAGAGAGGACGACACCC

Downstream 100 bases:

>100_bases
GGCAGTACGGGCATGGTTCGCAGCCATGCCCGCGTTTTTTCGCCCGCCGCGCAGCGCCCCGCGCGACGGGCTATCCGGAT
AAGGAGGCCGGCGGCGGCGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MARTAPAASFESLESDLDQKFAYPASSKTYIPGSRPDIRVPMRTILQTSTRTEKGEMPNPPIPVYDTSGPYSDPDVHIDL
KAGLPALREKWIAERGDTEVLPGLSSEYGRDRANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRE
SLNLQALYDKPEYKALLRQHPGNALGAGLPLRPEDITPEFVRNEIATGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMFRDTLIEQ
AEQGVDYFTIHAGVLLRYVPLTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIA
DSNDDAQFGELRTLGELTAKAWKHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERA
RSYHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPKEAQQGMEEKSIEFLKKGSKIYS

Sequences:

>Translated_626_residues
MARTAPAASFESLESDLDQKFAYPASSKTYIPGSRPDIRVPMRTILQTSTRTEKGEMPNPPIPVYDTSGPYSDPDVHIDL
KAGLPALREKWIAERGDTEVLPGLSSEYGRDRANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRE
SLNLQALYDKPEYKALLRQHPGNALGAGLPLRPEDITPEFVRNEIATGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMFRDTLIEQ
AEQGVDYFTIHAGVLLRYVPLTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIA
DSNDDAQFGELRTLGELTAKAWKHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERA
RSYHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPKEAQQGMEEKSIEFLKKGSKIYS
>Mature_625_residues
ARTAPAASFESLESDLDQKFAYPASSKTYIPGSRPDIRVPMRTILQTSTRTEKGEMPNPPIPVYDTSGPYSDPDVHIDLK
AGLPALREKWIAERGDTEVLPGLSSEYGRDRANDPATAHLRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRES
LNLQALYDKPEYKALLRQHPGNALGAGLPLRPEDITPEFVRNEIATGRAIIPANINHTELEPMAIGRNFRVKINGNLGNS
AVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDKTGGIAEDLTWEMFRDTLIEQA
EQGVDYFTIHAGVLLRYVPLTADRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIAD
SNDDAQFGELRTLGELTAKAWKHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGAA
NIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSKARFEFRWEDQFNLGLDPERAR
SYHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAASLPKEAQQGMEEKSIEFLKKGSKIYS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=609, Percent_Identity=69.1297208538588, Blast_Score=880, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69537; Mature: 69406

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTAPAASFESLESDLDQKFAYPASSKTYIPGSRPDIRVPMRTILQTSTRTEKGEMPNP
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
PIPVYDTSGPYSDPDVHIDLKAGLPALREKWIAERGDTEVLPGLSSEYGRDRANDPATAH
CCCEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCHHE
LRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRESLNLQALYDKPEYKALLRQH
EEEHHHCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHC
PGNALGAGLPLRPEDITPEFVRNEIATGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEECCCCCC
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDK
HHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TGGIAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTADRVTGIVSRGGSIMAKW
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDDAQFGELRTLGELTAK
HHHHHCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AWKHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
HHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCC
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSK
CCCCHHCEEEEEEECCHHHCCCCCHHHHHCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHH
ARFEFRWEDQFNLGLDPERARSYHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAA
CEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
SLPKEAQQGMEEKSIEFLKKGSKIYS
CCCHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARTAPAASFESLESDLDQKFAYPASSKTYIPGSRPDIRVPMRTILQTSTRTEKGEMPNP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
PIPVYDTSGPYSDPDVHIDLKAGLPALREKWIAERGDTEVLPGLSSEYGRDRANDPATAH
CCCEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCHHE
LRFAQLTNPRRAKAGANVSQMHYARKGIITPEMEYVALRESLNLQALYDKPEYKALLRQH
EEEHHHCCCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHC
PGNALGAGLPLRPEDITPEFVRNEIATGRAIIPANINHTELEPMAIGRNFRVKINGNLGN
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEECCCCCC
SAVTSSLAEEVEKMVWSIRWGADTIMDLSTGKHIHETREWILRNSPVPIGTVPIYQALDK
HHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
TGGIAEDLTWEMFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTADRVTGIVSRGGSIMAKW
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
CLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIADSNDDAQFGELRTLGELTAK
HHHHHCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AWKHDVQVMIEGPGHVPLQRIQANMDEELKHCYEAPFYTLGPLVTDIAPGYDHITSGIGA
HHCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCCCC
ANIGWMGTAMLCYVTPKEHLGLPDKEDVREGIITYKIAAHAADLAKGWPGAQLRDNALSK
CCCCHHCEEEEEEECCHHHCCCCCHHHHHCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHH
ARFEFRWEDQFNLGLDPERARSYHDATLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYAA
CEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
SLPKEAQQGMEEKSIEFLKKGSKIYS
CCCHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]