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The map label for this gene is alkJ [H]

Identifier: 73539909

GI number: 73539909

Start: 228248

End: 229927

Strand: Direct

Name: alkJ [H]

Synonym: Reut_A0203

Alternate gene names: 73539909

Gene position: 228248-229927 (Clockwise)

Preceding gene: 73539908

Following gene: 73539911

Centisome position: 6.0

GC content: 67.38

Gene sequence:

>1680_bases
ATGGAAACCACGTATGACTATGTCGTTGTCGGCGCGGGTTCGGCGGGGTGTGCGCTGGCCGGGCGCCTCGCTGACAGCGG
CAGCGACAGTATCGCGCTGGTCGAGGCGGGCCATCACGACCACCATGTGCTGGTGCGCACACCCGCGGGCATCGGCGCCA
TGCTGCCGCGCGGCGGGGCCCGCAACTACGCCTATGAAACCGTGCCGCAGCCCGGACTGAACGGCAGGCGCGGCTTCCAG
CCACGGGGCCGCGGGCTCGGTGGCTCCTCATCGATCAACGCCATGATCTACACGCGCGGCCGCCCCGCCGACTACGACGC
GTGGGCCGCCGCAGGCTGCGACGGGTGGTCATGGGACGATGTGCTGCCGTACTTTCGCCGCGCCGAGTGCAATGCGCGCG
TGGCCGGCAGCGACGAGGATCCACTGCATGGCGGCACGGGCCCGCTCCACGTATCGGACCTGCGCACGCCCAATCCATTC
GGCCAGCATTTCATCGACGCCGCCCAGCAGGCCGGCTTCACGCGCAACGACGATTTCAACGGCGAGGAGCAGGAAGGCGT
CGGCTGGTACCAGGTTACGCAGCACGAAGGCGAGCGCTGGAACGCCGCGCGCGCTTACCTGCATGGCGGCAATGCACGCG
ACAAGGCCTGTAACGGCGGACGTGCCGGCCTGCACGTGCTGACCGGCATGCAGGTGCTGCGTATTGTCTTCGAGGGCAGG
CATGCGGTAGGCGTGCTGGCTGTGCGCAATGGCCGCCAGCACCTGCTTCGCGCTCGTCGGGACGTCATCGTGTCGGCTGG
CACCTTCGGCTCGCCGCAGCTGCTGATGGCCTCCGGCATCGGCCCCGCGGCGCACCTGCGCGATCTCGGTATTGCTGTGG
TGCACGACCTGCCCGGTGTCGGCGGCAACCTCCAGGACCATCTCGACGTCATCCTGCAGAAGCGCGTGCCCGCGCTGGAG
CTGTTTGGCCTGTCGCTCAGCGGTGCCATGCACTTGCTGTCCGAGATGCTGCGCTACCGGCGCGAGCGCGCGGGCATGAT
GAGTTCCAACTTTGCCGAGGCCGGCGGCTTCATCCGCAGCCATCCCGACCTGGCAGAACCGGACCTGCAACTGCATTTCG
TGGTGGGCATGGCCGACGACCACATGCGCAAGATCCGCTTCGGCCACGGTTATTCATGCCATGTGTGCGTGCTGCGTCCG
CGCAGCCGCGGTGAAGTCCGGCTCGATTCGCCCGATATGCGGCAGCCGCCGTGCATCGATCCCGGCTACTTGTCAGATCC
GCGCGACCTGGACGATATGGTGGCCGGCGTGAAAATCGTGCGCCGGATCTTCAATCAGCCGCAGCTTCTGTGCTTCGGCG
GGCGCGAGTTGTATTCCGCGCACCTGCGTGCCGACGACAGCGACGACGCGGCCGTGCGCGAGATGATCCGCGAACGCGCG
GACACCATCTACCATCCCGTGGGCACGTGCCGCATGGGCATGGACGCAATGGCGGTGGTCGATCCGCAATTGCGCGTGCG
CGGTGTGGAAGGTCTGCGCGTGGTCGATGCGTCGATCATGCCGACGCTGATCGGCGGCAATACCAATGCACCCGCCATCA
TGATCGGCGAGCGCGCGCATGACCTGATCCGCTATGCGCCGCGCGTACTGCTCAGGATTCGCGAGCCGATGGAGGCCTGA

Upstream 100 bases:

>100_bases
CGGCGGCAGCGCCTACACTGGTTTTCAGGCTGCCATCCTTCCGGGTGGGCGGGCAGAGGGCATACGCCCCGCCGCCCTCT
CCGGACACGCGGGGAGACTG

Downstream 100 bases:

>100_bases
GTCGCTTCGCGGGTTTCACGCGGTCCAGCCGCGTTCGAATACGCGTTTCAGCACTTCGGGCTCCATGTCGAGCGTGCCGA
ACACGCGCCCGTGCTGCCGG

Product: glucose-methanol-choline oxidoreductase:GMC oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
METTYDYVVVGAGSAGCALAGRLADSGSDSIALVEAGHHDHHVLVRTPAGIGAMLPRGGARNYAYETVPQPGLNGRRGFQ
PRGRGLGGSSSINAMIYTRGRPADYDAWAAAGCDGWSWDDVLPYFRRAECNARVAGSDEDPLHGGTGPLHVSDLRTPNPF
GQHFIDAAQQAGFTRNDDFNGEEQEGVGWYQVTQHEGERWNAARAYLHGGNARDKACNGGRAGLHVLTGMQVLRIVFEGR
HAVGVLAVRNGRQHLLRARRDVIVSAGTFGSPQLLMASGIGPAAHLRDLGIAVVHDLPGVGGNLQDHLDVILQKRVPALE
LFGLSLSGAMHLLSEMLRYRRERAGMMSSNFAEAGGFIRSHPDLAEPDLQLHFVVGMADDHMRKIRFGHGYSCHVCVLRP
RSRGEVRLDSPDMRQPPCIDPGYLSDPRDLDDMVAGVKIVRRIFNQPQLLCFGGRELYSAHLRADDSDDAAVREMIRERA
DTIYHPVGTCRMGMDAMAVVDPQLRVRGVEGLRVVDASIMPTLIGGNTNAPAIMIGERAHDLIRYAPRVLLRIREPMEA

Sequences:

>Translated_559_residues
METTYDYVVVGAGSAGCALAGRLADSGSDSIALVEAGHHDHHVLVRTPAGIGAMLPRGGARNYAYETVPQPGLNGRRGFQ
PRGRGLGGSSSINAMIYTRGRPADYDAWAAAGCDGWSWDDVLPYFRRAECNARVAGSDEDPLHGGTGPLHVSDLRTPNPF
GQHFIDAAQQAGFTRNDDFNGEEQEGVGWYQVTQHEGERWNAARAYLHGGNARDKACNGGRAGLHVLTGMQVLRIVFEGR
HAVGVLAVRNGRQHLLRARRDVIVSAGTFGSPQLLMASGIGPAAHLRDLGIAVVHDLPGVGGNLQDHLDVILQKRVPALE
LFGLSLSGAMHLLSEMLRYRRERAGMMSSNFAEAGGFIRSHPDLAEPDLQLHFVVGMADDHMRKIRFGHGYSCHVCVLRP
RSRGEVRLDSPDMRQPPCIDPGYLSDPRDLDDMVAGVKIVRRIFNQPQLLCFGGRELYSAHLRADDSDDAAVREMIRERA
DTIYHPVGTCRMGMDAMAVVDPQLRVRGVEGLRVVDASIMPTLIGGNTNAPAIMIGERAHDLIRYAPRVLLRIREPMEA
>Mature_559_residues
METTYDYVVVGAGSAGCALAGRLADSGSDSIALVEAGHHDHHVLVRTPAGIGAMLPRGGARNYAYETVPQPGLNGRRGFQ
PRGRGLGGSSSINAMIYTRGRPADYDAWAAAGCDGWSWDDVLPYFRRAECNARVAGSDEDPLHGGTGPLHVSDLRTPNPF
GQHFIDAAQQAGFTRNDDFNGEEQEGVGWYQVTQHEGERWNAARAYLHGGNARDKACNGGRAGLHVLTGMQVLRIVFEGR
HAVGVLAVRNGRQHLLRARRDVIVSAGTFGSPQLLMASGIGPAAHLRDLGIAVVHDLPGVGGNLQDHLDVILQKRVPALE
LFGLSLSGAMHLLSEMLRYRRERAGMMSSNFAEAGGFIRSHPDLAEPDLQLHFVVGMADDHMRKIRFGHGYSCHVCVLRP
RSRGEVRLDSPDMRQPPCIDPGYLSDPRDLDDMVAGVKIVRRIFNQPQLLCFGGRELYSAHLRADDSDDAAVREMIRERA
DTIYHPVGTCRMGMDAMAVVDPQLRVRGVEGLRVVDASIMPTLIGGNTNAPAIMIGERAHDLIRYAPRVLLRIREPMEA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=558, Percent_Identity=43.7275985663082, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI1786503, Length=553, Percent_Identity=41.5913200723327, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17532301, Length=567, Percent_Identity=36.5079365079365, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24642055, Length=590, Percent_Identity=35.4237288135593, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24642037, Length=581, Percent_Identity=36.3166953528399, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI45551458, Length=587, Percent_Identity=35.7751277683135, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI45549471, Length=587, Percent_Identity=35.7751277683135, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24642035, Length=593, Percent_Identity=35.7504215851602, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24642042, Length=580, Percent_Identity=36.3793103448276, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI18859995, Length=582, Percent_Identity=35.7388316151203, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24642039, Length=581, Percent_Identity=35.1118760757315, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24642048, Length=583, Percent_Identity=33.7907375643225, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24642059, Length=582, Percent_Identity=34.192439862543, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24650267, Length=581, Percent_Identity=33.3907056798623, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI18859993, Length=584, Percent_Identity=35.1027397260274, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17137792, Length=573, Percent_Identity=32.8097731239092, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24642051, Length=582, Percent_Identity=32.1305841924399, Blast_Score=245, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24642057, Length=606, Percent_Identity=29.8679867986799, Blast_Score=152, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24645930, Length=586, Percent_Identity=27.4744027303754, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 60877; Mature: 60877

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTYDYVVVGAGSAGCALAGRLADSGSDSIALVEAGHHDHHVLVRTPAGIGAMLPRGGA
CCCCEEEEEEECCCCCCCEEHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCC
RNYAYETVPQPGLNGRRGFQPRGRGLGGSSSINAMIYTRGRPADYDAWAAAGCDGWSWDD
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHH
VLPYFRRAECNARVAGSDEDPLHGGTGPLHVSDLRTPNPFGQHFIDAAQQAGFTRNDDFN
HHHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GEEQEGVGWYQVTQHEGERWNAARAYLHGGNARDKACNGGRAGLHVLTGMQVLRIVFEGR
CCCCCCCCEEEEECCCCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
HAVGVLAVRNGRQHLLRARRDVIVSAGTFGSPQLLMASGIGPAAHLRDLGIAVVHDLPGV
CEEEEEEECCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCCC
GGNLQDHLDVILQKRVPALELFGLSLSGAMHLLSEMLRYRRERAGMMSSNFAEAGGFIRS
CCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
HPDLAEPDLQLHFVVGMADDHMRKIRFGHGYSCHVCVLRPRSRGEVRLDSPDMRQPPCID
CCCCCCCCEEEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCC
PGYLSDPRDLDDMVAGVKIVRRIFNQPQLLCFGGRELYSAHLRADDSDDAAVREMIRERA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHH
DTIYHPVGTCRMGMDAMAVVDPQLRVRGVEGLRVVDASIMPTLIGGNTNAPAIMIGERAH
HHHHCCCHHHHCCCCEEEEECCCCEEECCCCEEEECCHHCCEEECCCCCCCEEEECHHHH
DLIRYAPRVLLRIREPMEA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METTYDYVVVGAGSAGCALAGRLADSGSDSIALVEAGHHDHHVLVRTPAGIGAMLPRGGA
CCCCEEEEEEECCCCCCCEEHHHCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCC
RNYAYETVPQPGLNGRRGFQPRGRGLGGSSSINAMIYTRGRPADYDAWAAAGCDGWSWDD
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHH
VLPYFRRAECNARVAGSDEDPLHGGTGPLHVSDLRTPNPFGQHFIDAAQQAGFTRNDDFN
HHHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GEEQEGVGWYQVTQHEGERWNAARAYLHGGNARDKACNGGRAGLHVLTGMQVLRIVFEGR
CCCCCCCCEEEEECCCCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
HAVGVLAVRNGRQHLLRARRDVIVSAGTFGSPQLLMASGIGPAAHLRDLGIAVVHDLPGV
CEEEEEEECCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCCC
GGNLQDHLDVILQKRVPALELFGLSLSGAMHLLSEMLRYRRERAGMMSSNFAEAGGFIRS
CCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
HPDLAEPDLQLHFVVGMADDHMRKIRFGHGYSCHVCVLRPRSRGEVRLDSPDMRQPPCID
CCCCCCCCEEEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCC
PGYLSDPRDLDDMVAGVKIVRRIFNQPQLLCFGGRELYSAHLRADDSDDAAVREMIRERA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHH
DTIYHPVGTCRMGMDAMAVVDPQLRVRGVEGLRVVDASIMPTLIGGNTNAPAIMIGERAH
HHHHCCCHHHHCCCCEEEEECCCCEEECCCCEEEECCHHCCEEECCCCCCCEEEECHHHH
DLIRYAPRVLLRIREPMEA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]