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The map label for this gene is metK [H]

Identifier: 73539907

GI number: 73539907

Start: 225865

End: 227028

Strand: Direct

Name: metK [H]

Synonym: Reut_A0201

Alternate gene names: 73539907

Gene position: 225865-227028 (Clockwise)

Preceding gene: 73539902

Following gene: 73539908

Centisome position: 5.93

GC content: 65.38

Gene sequence:

>1164_bases
GTGGCAAACGACTTCCTTTTCACTTCGGAGTCCGTCTCCGAAGGCCATCCCGACAAGGTCGCAGACCAGATTTCCGACGC
CGTCCTCGATGCCATCCTGGCGCAGGACAAGTACGCGCGGGTGGCTGCCGAGACGCTCTGCAACACCGGGCTGGTCGTGC
TGGCCGGGGAAATCACCACCACGGCCAACGTCGACTACATCCAGGTCGCGCGCGACACGATCAAGCGCATCGGCTACGAC
AACACCGAGTACGGCATCGACTACAAGGGTTGCGCGGTGCTGGTCGCTTATGACAAGCAGTCGCCCGACATCGCCCAGGG
CGTGGACCGCGCCTCGGACGACTACCTGAACCAGGGTGCCGGCGATCAGGGCCTGATGTTCGGCTACGCCTGCGACGAGA
CCCCGGAACTGATGCCGTTCCCGATCTACTACTCGCACCGTCTGGTCGAGCGCCAGTCGCAGCTGCGCCGCGATGGCCGT
CTGCCGTGGCTGCGCCCGGACGCCAAGTCGCAGGTCACCGTCCGTTATGTCGACGGCCGTCCGCACAGTGTCGACACCGT
CGTGCTGTCGACCCAGCATGCGCCGGACATCACCCAGGCCCAGATCCGCGAAGCGGTGATCGAGGAGATCATCAAGCCGG
TGCTGCCGCCCGAGATGCTCAAGGACACCAAGTACCTGGTCAACCCGACCGGTCGCTTCGTCATCGGCGGCCCGCAGGGC
GATTGCGGCTTGACCGGCCGCAAGATCATCGTCGACACGTACGGTGGCGCTTCGCCGCACGGCGGCGGTGCGTTCTCGGG
CAAGGACCCGTCGAAGGTCGACCGTTCGGCCGCCTACGCCGCGCGCTACGTTGCCAAGAACGTCGTGGCCGCGGGCCTCG
CGCGCCAGTGCCAGGTGCAGGTGAGCTATGCCATCGGCGTGGCGCGTCCGATCAACGTGACGGTCTACACCGAAGGCACG
GGCAAGATTTCCGACGCGAAGATCGCCGAACTGGTCCAGGAACACTTCGACCTGCGTCCGAAGGGCATCGTGCAGATGCT
CGACCTGCTGCGCCCGATCTACGAGAAGACTGCCGCTTACGGCCACTTTGGCCGCGAAGAGCCGGAATTCTCGTGGGAAG
CGACGGACAAGGCTGCGATCCTGCGCGCAGCGGCTGGTCTGTAA

Upstream 100 bases:

>100_bases
GGCGATACCGCTCTAACCGAGCGGCCTGAAAATACCGCTAAAGCGTCGCCGCCATGACCCGACAGGGTGGCACGCAATAG
CCAACCTGGAGAACAAGTTC

Downstream 100 bases:

>100_bases
GCATTCGCCTTTCTGGCATGAAACCCCGCCGGCCAATGGTCGGCGGGGTTTTTTGTTGGGCGCTTTGCGGCCGCTATGAC
TGTGATCAATTAGTCACCAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MANDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIQVARDTIKRIGYD
NTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYSHRLVERQSQLRRDGR
LPWLRPDAKSQVTVRYVDGRPHSVDTVVLSTQHAPDITQAQIREAVIEEIIKPVLPPEMLKDTKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINVTVYTEGT
GKISDAKIAELVQEHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKAAILRAAAGL

Sequences:

>Translated_387_residues
MANDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIQVARDTIKRIGYD
NTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYSHRLVERQSQLRRDGR
LPWLRPDAKSQVTVRYVDGRPHSVDTVVLSTQHAPDITQAQIREAVIEEIIKPVLPPEMLKDTKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINVTVYTEGT
GKISDAKIAELVQEHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKAAILRAAAGL
>Mature_386_residues
ANDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITTTANVDYIQVARDTIKRIGYDN
TEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYSHRLVERQSQLRRDGRL
PWLRPDAKSQVTVRYVDGRPHSVDTVVLSTQHAPDITQAQIREAVIEEIIKPVLPPEMLKDTKYLVNPTGRFVIGGPQGD
CGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINVTVYTEGTG
KISDAKIAELVQEHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGREEPEFSWEATDKAAILRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=59.7368421052632, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=58.9473684210526, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=68.1462140992167, Blast_Score=527, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=57.5447570332481, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=378, Percent_Identity=58.994708994709, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=378, Percent_Identity=58.4656084656085, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=56.3492063492063, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=378, Percent_Identity=52.9100529100529, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=56.6666666666667, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=54.8104956268222, Blast_Score=353, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=56.1056105610561, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=63.0952380952381, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=84.2105263157895, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=59.2105263157895, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=59.5238095238095, Blast_Score=426, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=54.5212765957447, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=53.9893617021277, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=53.9893617021277, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=51.4588859416446, Blast_Score=351, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=57.0247933884297, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42159; Mature: 42028

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYSHRLVERQSQLRRDGRLPWLRPDAKSQVTVRYVDGR
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPDITQAQIREAVIEEIIKPVLPPEMLKDTKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINVTVYTEGTGKISDAKIAELVQEHFDLRPKGIVQMLDLLRPIYEKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPEFSWEATDKAAILRAAAGL
CCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ANDFLFTSESVSEGHPDKVADQISDAVLDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYSHRLVERQSQLRRDGRLPWLRPDAKSQVTVRYVDGR
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPDITQAQIREAVIEEIIKPVLPPEMLKDTKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGASPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINVTVYTEGTGKISDAKIAELVQEHFDLRPKGIVQMLDLLRPIYEKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREEPEFSWEATDKAAILRAAAGL
CCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA