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The map label for this gene is thlA [H]

Identifier: 73539844

GI number: 73539844

Start: 164652

End: 165833

Strand: Direct

Name: thlA [H]

Synonym: Reut_A0138

Alternate gene names: 73539844

Gene position: 164652-165833 (Clockwise)

Preceding gene: 73539843

Following gene: 73539845

Centisome position: 4.33

GC content: 67.77

Gene sequence:

>1182_bases
ATGCAAGACCCGATCGTCATCGTTTCCGCCGCCCGCACCCCCATGGCGGCTTTCCAGGGCGAGTTCGCATCGCTGACGGC
GCCGCAGCTCGGCGCGGTCGCCATCCGCGCCGCAGTGGAACGCGCAGGCATCAAGCCCGAACAGGTCGAGGAAGTGGTGT
TCGGCTGCGTGTTGCCGGCCGGTCAGGGCCAGGCGCCCGCACGACAGGCGGCGCTCGGCGCGGGTCTGCCGCTCAGCGTT
GGCTGCACCACCGTCAACAAGATGTGCGGCTCGGGCATGCGTGCGGCCATGAACGTCTATGACGGGCTGGTGGCGGGCTC
GTTCGACATCGCCGTTGCCGGCGGCATGGAGAGCATGACCAACGCGCCGTACCTGATCCCGAAGGGGCGCGGCGGTTACC
GCATCGGCCACGGCATGATGTATGACCACATGATGCTCGACGGCCTCGAAGACGCGTACGACAAGGGCCGCGCGATGGGT
ACCTTCGGCGAAGACTGCGCCGCCAAGTACGGCTTCACGCGCGAGCAGCAGGACACCTTCGCGATCGAAAGCGTGCGCCG
CGCGCAGCAGGCCACCGAGGCTGGCGACTTCCGCTGGGAGATCGCGCCGGTCACCGTGTCGGGCAAGGGCGGCGACACCG
TCATCGATACCGATGAAGGCCCGCGCCGCATCAAGACCGACAAGATCCCGTCGCTGAAGCCGGCGTTCGCCAAGGACGGC
ACCATCACCGCCGCATCGTCGTCGTCGATCAACGACGGCGCCTCGGCGCTGGTGATGATGCGCGAATCGACCGCCAAGAA
ACTGGGCCTGCGGCCCATCGCACGCATGCTTGGCCACACCACGCACGCGCAGGCGCCGGGCTGGTTCACCACCGCACCGG
TCGAGGCCATGGCCAAGCTGTACCGCAAGCTGGACTGGACCACCGACAGCATCGACCTGTTCGAGATCAACGAGGCCTTC
GCCGTGGTGCCGATGGCTGCGATGCACGACCTCAAGATCCCGCGCGAGAAGGTCAATATCCACGGCGGCGCGTGTGCGCT
GGGCCATCCGATCGGCGCCTCGGGCGCACGCATCATGACCACGCTGATCGGCGCGCTGCGCAAGACGGGCGGCAAGCGTG
GCGTGGCCAGCCTGTGCATTGGTGGCGGAGAGGCGACGGCGGTTGGTATCGAGATCGTCTGA

Upstream 100 bases:

>100_bases
CATCATGTAGTCATCGAATCAAACACCGTCGTTTCCGTGCGCGTACCCTTACCCGCACGGAGCGACGGTGGTGAAGCATA
CCGATATCAGGAGAACAGAC

Downstream 100 bases:

>100_bases
TTCAACCCAGCATGTTCCCCCCTCTCCCGCTTGCGAAAGAGGGGGGGGCAAACAACCCGATCATCTGAACCTCGCCTCAG
GAGATAACCCTTGCCAACCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQDPIVIVSAARTPMAAFQGEFASLTAPQLGAVAIRAAVERAGIKPEQVEEVVFGCVLPAGQGQAPARQAALGAGLPLSV
GCTTVNKMCGSGMRAAMNVYDGLVAGSFDIAVAGGMESMTNAPYLIPKGRGGYRIGHGMMYDHMMLDGLEDAYDKGRAMG
TFGEDCAAKYGFTREQQDTFAIESVRRAQQATEAGDFRWEIAPVTVSGKGGDTVIDTDEGPRRIKTDKIPSLKPAFAKDG
TITAASSSSINDGASALVMMRESTAKKLGLRPIARMLGHTTHAQAPGWFTTAPVEAMAKLYRKLDWTTDSIDLFEINEAF
AVVPMAAMHDLKIPREKVNIHGGACALGHPIGASGARIMTTLIGALRKTGGKRGVASLCIGGGEATAVGIEIV

Sequences:

>Translated_393_residues
MQDPIVIVSAARTPMAAFQGEFASLTAPQLGAVAIRAAVERAGIKPEQVEEVVFGCVLPAGQGQAPARQAALGAGLPLSV
GCTTVNKMCGSGMRAAMNVYDGLVAGSFDIAVAGGMESMTNAPYLIPKGRGGYRIGHGMMYDHMMLDGLEDAYDKGRAMG
TFGEDCAAKYGFTREQQDTFAIESVRRAQQATEAGDFRWEIAPVTVSGKGGDTVIDTDEGPRRIKTDKIPSLKPAFAKDG
TITAASSSSINDGASALVMMRESTAKKLGLRPIARMLGHTTHAQAPGWFTTAPVEAMAKLYRKLDWTTDSIDLFEINEAF
AVVPMAAMHDLKIPREKVNIHGGACALGHPIGASGARIMTTLIGALRKTGGKRGVASLCIGGGEATAVGIEIV
>Mature_393_residues
MQDPIVIVSAARTPMAAFQGEFASLTAPQLGAVAIRAAVERAGIKPEQVEEVVFGCVLPAGQGQAPARQAALGAGLPLSV
GCTTVNKMCGSGMRAAMNVYDGLVAGSFDIAVAGGMESMTNAPYLIPKGRGGYRIGHGMMYDHMMLDGLEDAYDKGRAMG
TFGEDCAAKYGFTREQQDTFAIESVRRAQQATEAGDFRWEIAPVTVSGKGGDTVIDTDEGPRRIKTDKIPSLKPAFAKDG
TITAASSSSINDGASALVMMRESTAKKLGLRPIARMLGHTTHAQAPGWFTTAPVEAMAKLYRKLDWTTDSIDLFEINEAF
AVVPMAAMHDLKIPREKVNIHGGACALGHPIGASGARIMTTLIGALRKTGGKRGVASLCIGGGEATAVGIEIV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=46.5295629820051, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=44.1326530612245, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=34.7043701799486, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=35.9493670886076, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI4504327, Length=434, Percent_Identity=31.3364055299539, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI194353979, Length=389, Percent_Identity=29.305912596401, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=46.9072164948454, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=40.5612244897959, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1787663, Length=397, Percent_Identity=33.7531486146096, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI48994986, Length=403, Percent_Identity=34.9875930521092, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1788683, Length=416, Percent_Identity=29.8076923076923, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=42.7835051546392, Blast_Score=313, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=43.7017994858612, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=37.9487179487179, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=34.1836734693878, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=31.7535545023697, Blast_Score=177, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=45.5, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6322031, Length=392, Percent_Identity=33.1632653061224, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=43.5567010309278, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=45.7583547557841, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=34.9367088607595, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=30.5687203791469, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41148; Mature: 41148

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDPIVIVSAARTPMAAFQGEFASLTAPQLGAVAIRAAVERAGIKPEQVEEVVFGCVLPA
CCCCEEEEECCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
GQGQAPARQAALGAGLPLSVGCTTVNKMCGSGMRAAMNVYDGLVAGSFDIAVAGGMESMT
CCCCCCHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHHC
NAPYLIPKGRGGYRIGHGMMYDHMMLDGLEDAYDKGRAMGTFGEDCAAKYGFTREQQDTF
CCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHH
AIESVRRAQQATEAGDFRWEIAPVTVSGKGGDTVIDTDEGPRRIKTDKIPSLKPAFAKDG
HHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
TITAASSSSINDGASALVMMRESTAKKLGLRPIARMLGHTTHAQAPGWFTTAPVEAMAKL
EEEECCCCCCCCCCCEEEEEHHHHHHHHCHHHHHHHHCCCCCCCCCCCEECCCHHHHHHH
YRKLDWTTDSIDLFEINEAFAVVPMAAMHDLKIPREKVNIHGGACALGHPIGASGARIMT
HHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHEEECCCEEECCCCCCCCCHHHHH
TLIGALRKTGGKRGVASLCIGGGEATAVGIEIV
HHHHHHHHCCCCCCCHHEEECCCCEEEEEEEEC
>Mature Secondary Structure
MQDPIVIVSAARTPMAAFQGEFASLTAPQLGAVAIRAAVERAGIKPEQVEEVVFGCVLPA
CCCCEEEEECCCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
GQGQAPARQAALGAGLPLSVGCTTVNKMCGSGMRAAMNVYDGLVAGSFDIAVAGGMESMT
CCCCCCHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHHC
NAPYLIPKGRGGYRIGHGMMYDHMMLDGLEDAYDKGRAMGTFGEDCAAKYGFTREQQDTF
CCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCHHHH
AIESVRRAQQATEAGDFRWEIAPVTVSGKGGDTVIDTDEGPRRIKTDKIPSLKPAFAKDG
HHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
TITAASSSSINDGASALVMMRESTAKKLGLRPIARMLGHTTHAQAPGWFTTAPVEAMAKL
EEEECCCCCCCCCCCEEEEEHHHHHHHHCHHHHHHHHCCCCCCCCCCCEECCCHHHHHHH
YRKLDWTTDSIDLFEINEAFAVVPMAAMHDLKIPREKVNIHGGACALGHPIGASGARIMT
HHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHEEECCCEEECCCCCCCCCHHHHH
TLIGALRKTGGKRGVASLCIGGGEATAVGIEIV
HHHHHHHHCCCCCCCHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]