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The map label for this gene is oprM [H]

Identifier: 73539464

GI number: 73539464

Start: 2466473

End: 2467918

Strand: Direct

Name: oprM [H]

Synonym: Reut_B5642

Alternate gene names: 73539464

Gene position: 2466473-2467918 (Clockwise)

Preceding gene: 73539460

Following gene: 73539470

Centisome position: 90.47

GC content: 68.81

Gene sequence:

>1446_bases
ATGTGTTCTGCCCGTCTCCTCGCCCCTTTACTGAGTGCCGCCGTCGTGGCGGGCTGCGCGGTCGGCCCCGACTATCACGC
GCCAGATGTCCCGTTGTCGGAGCGATACCTGGGCCAGCCGCAGGTCGAGCAGCGCCGGGCCGCATCCACGGCGGAACTCG
TGAACTGGTGGGAGGGGTTTGGCGATCCGCAACTGACCCGCCTGGTGTCGGTCGCACTCGACCAGAACCTGGATCTGGCC
CAGGCGACGGCACGCGTCGCCCAGGCTCGCGCCGGGCTCGGTGCCGCCACCGCGGCGTTGCTGCCTTCGGCCGGCGTCAG
CGCACAGGCGGCCCGAAGCTACCAGTCGATCGAAACACCGCTGGGGAGGCTGCAAAGCGCGACGCAGGGCTTTGACCGGT
ATGGCAACAATTACGAAGCGAATTTGGGCGCGTCCTGGGAACTGGACGTGTTCGGCGGCTTGCGGCGCGGGCGCGAGGCT
GCGCTCGCTGACTATCGCGCATCGGAAGCGGGCGCCATGGCCACGCGGCTTGCGGTGGCCGCGCAGACCGCGGACCTGTA
CATCACGGTCCGCGGCCTGCAGGCCCGCCTTGCCATTGCCACGCGGCAGGCGGAGACGCAACAGAAGCTGGTGTCGACTG
TTGGCCTGCTGTATGGCAAGGGCCTCGCGGCAGAACTGGAAGTGCGGCAGGCCGAAGGCGCGCTGGCCGAGGTTCAGGCC
ACCATACCGGTACTCGAGGCCGGACTGGATGCGGCGCTGAACGCGCTCGACGTCATGCTCGGCGTGCCGCCCGGCACATA
TCGCGCGGAACTGCAGGGTGTGCAAGACATACCCGCGGCCCCGGAGATCACTGGCTCGGGGTCGCCCGCAGACCTGCTGC
GGCGCCGGCCCGATCTGGTCGTCGCGGAGCGGCGGCTGGCAGCCGCCAATGCACGCATCGGGGTCGCCATTGCGGAGTAC
TACCCGAAATTTTCATTGGGGGCGTTGCTGGGCAGCGCCACCACCGTGGCCAGCGGCAATCTCTTTTCCGCTGGCGCGAG
CCAGGCAGCAGGCGTAGCGGGCTTGCGCTGGCGCCTGTTCGATTTCGGCCGCATCAACGCGCAGATTGAACAGGCGCAGG
GACAGGAGGCGGAATCGCTCGCCGCGTATCGCCAGGCCGTGCTGCACGCCACGGAAGATGTCGAGAACGCCTTCTCCGCG
CTGGTCAAGCGCGAGAACCAGGCACAGACCCTGGCACGCGGGGAGGCGTCGCTGTCCCAGGCGCGCGATGCATCGTTTGC
CGCCTACCAGAAAGGCGTCGTCCGCCTGATCGAGGTCCTGCATGCCGACGAGCGGCTCCTGCAAACCTCCGATGCAAGGG
CTCAGGCCCGGACCGAATCGGCGCGGGCCGCGGTTGCGGCATTCAAGGCGCTCGGTGGCGGATGGCAGCCGCATGCGGCT
GGCTGA

Upstream 100 bases:

>100_bases
GTGGTCTCAGTCATGGTACGAGGGTACCGCGCGAATTGGGAGTTGACAACATGTGACCAATTTATAAAATCGTCACAACA
TCCTTTCAAGGTCCGCTGCC

Downstream 100 bases:

>100_bases
TGCTGCCTGGTTGCCCGTGCGGGCGGGCAGCAAGCCATAGCACGGCGCGTCGCGCAGCGGGTTGGCCGGCTCCTCTCTTG
ACCAGGGGTAGCCAGTCACA

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MCSARLLAPLLSAAVVAGCAVGPDYHAPDVPLSERYLGQPQVEQRRAASTAELVNWWEGFGDPQLTRLVSVALDQNLDLA
QATARVAQARAGLGAATAALLPSAGVSAQAARSYQSIETPLGRLQSATQGFDRYGNNYEANLGASWELDVFGGLRRGREA
ALADYRASEAGAMATRLAVAAQTADLYITVRGLQARLAIATRQAETQQKLVSTVGLLYGKGLAAELEVRQAEGALAEVQA
TIPVLEAGLDAALNALDVMLGVPPGTYRAELQGVQDIPAAPEITGSGSPADLLRRRPDLVVAERRLAAANARIGVAIAEY
YPKFSLGALLGSATTVASGNLFSAGASQAAGVAGLRWRLFDFGRINAQIEQAQGQEAESLAAYRQAVLHATEDVENAFSA
LVKRENQAQTLARGEASLSQARDASFAAYQKGVVRLIEVLHADERLLQTSDARAQARTESARAAVAAFKALGGGWQPHAA
G

Sequences:

>Translated_481_residues
MCSARLLAPLLSAAVVAGCAVGPDYHAPDVPLSERYLGQPQVEQRRAASTAELVNWWEGFGDPQLTRLVSVALDQNLDLA
QATARVAQARAGLGAATAALLPSAGVSAQAARSYQSIETPLGRLQSATQGFDRYGNNYEANLGASWELDVFGGLRRGREA
ALADYRASEAGAMATRLAVAAQTADLYITVRGLQARLAIATRQAETQQKLVSTVGLLYGKGLAAELEVRQAEGALAEVQA
TIPVLEAGLDAALNALDVMLGVPPGTYRAELQGVQDIPAAPEITGSGSPADLLRRRPDLVVAERRLAAANARIGVAIAEY
YPKFSLGALLGSATTVASGNLFSAGASQAAGVAGLRWRLFDFGRINAQIEQAQGQEAESLAAYRQAVLHATEDVENAFSA
LVKRENQAQTLARGEASLSQARDASFAAYQKGVVRLIEVLHADERLLQTSDARAQARTESARAAVAAFKALGGGWQPHAA
G
>Mature_481_residues
MCSARLLAPLLSAAVVAGCAVGPDYHAPDVPLSERYLGQPQVEQRRAASTAELVNWWEGFGDPQLTRLVSVALDQNLDLA
QATARVAQARAGLGAATAALLPSAGVSAQAARSYQSIETPLGRLQSATQGFDRYGNNYEANLGASWELDVFGGLRRGREA
ALADYRASEAGAMATRLAVAAQTADLYITVRGLQARLAIATRQAETQQKLVSTVGLLYGKGLAAELEVRQAEGALAEVQA
TIPVLEAGLDAALNALDVMLGVPPGTYRAELQGVQDIPAAPEITGSGSPADLLRRRPDLVVAERRLAAANARIGVAIAEY
YPKFSLGALLGSATTVASGNLFSAGASQAAGVAGLRWRLFDFGRINAQIEQAQGQEAESLAAYRQAVLHATEDVENAFSA
LVKRENQAQTLARGEASLSQARDASFAAYQKGVVRLIEVLHADERLLQTSDARAQARTESARAAVAAFKALGGGWQPHAA
G

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=481, Percent_Identity=28.2744282744283, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI2367348, Length=504, Percent_Identity=26.5873015873016, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50405; Mature: 50405

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSARLLAPLLSAAVVAGCAVGPDYHAPDVPLSERYLGQPQVEQRRAASTAELVNWWEGF
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCC
GDPQLTRLVSVALDQNLDLAQATARVAQARAGLGAATAALLPSAGVSAQAARSYQSIETP
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
LGRLQSATQGFDRYGNNYEANLGASWELDVFGGLRRGREAALADYRASEAGAMATRLAVA
HHHHHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQTADLYITVRGLQARLAIATRQAETQQKLVSTVGLLYGKGLAAELEVRQAEGALAEVQA
EECCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHH
TIPVLEAGLDAALNALDVMLGVPPGTYRAELQGVQDIPAAPEITGSGSPADLLRRRPDLV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHH
VAERRLAAANARIGVAIAEYYPKFSLGALLGSATTVASGNLFSAGASQAAGVAGLRWRLF
HHHHHHHHHCCHHEEHHHHHCCCHHHHHHHCCCHHEECCCCCCCCCCHHCCCCHHEEEEE
DFGRINAQIEQAQGQEAESLAAYRQAVLHATEDVENAFSALVKRENQAQTLARGEASLSQ
ECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ARDASFAAYQKGVVRLIEVLHADERLLQTSDARAQARTESARAAVAAFKALGGGWQPHAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
G
C
>Mature Secondary Structure
MCSARLLAPLLSAAVVAGCAVGPDYHAPDVPLSERYLGQPQVEQRRAASTAELVNWWEGF
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCC
GDPQLTRLVSVALDQNLDLAQATARVAQARAGLGAATAALLPSAGVSAQAARSYQSIETP
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
LGRLQSATQGFDRYGNNYEANLGASWELDVFGGLRRGREAALADYRASEAGAMATRLAVA
HHHHHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQTADLYITVRGLQARLAIATRQAETQQKLVSTVGLLYGKGLAAELEVRQAEGALAEVQA
EECCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHH
TIPVLEAGLDAALNALDVMLGVPPGTYRAELQGVQDIPAAPEITGSGSPADLLRRRPDLV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHH
VAERRLAAANARIGVAIAEYYPKFSLGALLGSATTVASGNLFSAGASQAAGVAGLRWRLF
HHHHHHHHHCCHHEEHHHHHCCCHHHHHHHCCCHHEECCCCCCCCCCHHCCCCHHEEEEE
DFGRINAQIEQAQGQEAESLAAYRQAVLHATEDVENAFSALVKRENQAQTLARGEASLSQ
ECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ARDASFAAYQKGVVRLIEVLHADERLLQTSDARAQARTESARAAVAAFKALGGGWQPHAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]