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The map label for this gene is 73539296

Identifier: 73539296

GI number: 73539296

Start: 2266695

End: 2268773

Strand: Reverse

Name: 73539296

Synonym: Reut_B5474

Alternate gene names: NA

Gene position: 2268773-2266695 (Counterclockwise)

Preceding gene: 73539297

Following gene: 73539295

Centisome position: 83.22

GC content: 65.9

Gene sequence:

>2079_bases
TTGACGCAACAGCGCAATACCGTCCGCGTAGCCACCGACGTGGGCGGCACCTTCACTGACCTCGTCTACTTCGAGTTCGA
TCCTGCCACGGGCACGCAACGCATCCGCACGGCAAAGTCCGACACCACGCCGCCCAACTTCGAGCAAGGCATCCTCAACG
TCCTGCGCAAGGCCGACGTCGATCTCGAAGAGGTCAGCTTCCTCGCACATGGCACCACCGTCGTGATCAATGCGCTGACC
GAGCGCCGCGGCGTGCGCACCGGCCTCATCACGACCGAAGGCTTTCGCGATGTGCTGGAGATCGGGCGCGCCAACCGTCC
CGACTTCTTCAACCTGAGCTGGGTCAAGCCATCGCCGTTCGTGCCGCGCTATCTGCGCCGCGAAGTGCCGGGCCGCATCC
ATTTCGATGGCGCAGAGCGCCAGTCGCTCGACCTGTCAGGCGTCGCGCAGATCATCCATGACTTCCGCAGCGAAGGCGTG
GAAGCCGTCGCCATCTGCCTGATGCACGCATACGCCAACCCCACGCACGAGCAGGCCGTGCTGGCCGAGGTTCGCAAGCT
GTGGCCCGAGGCCTCGGTCGTCGCGTCGCACCAGATCACGCGCGAATGGCGCGAATACGAGCGCACCAGCACGGCCGTGC
TCTCCGCTTACGTGCAGCCAATTGCAGCGCGCTATCTCAGCAACCTCGAAGACGGCCTGCGCGCAAATGGCTATCGCGCA
CCGCTGTATGTGATGCAGTCCAACTGCGGCGTCGACACCGTGGCGCGCGCACGCGAAATCCCCATCACGATGGTCGAATC
CGGTCCCGCCAGCGGCTTCTGGGGCGCGGCGGAACTCGGGCGCCTGATCGGCGAGCCGAACGTGCTCGCGCTCGATATCG
GCGGCACCACCGCCAAGTGCTCGCTGATCGAGAACGGACAGGTCGCCATCAAGACGGACTACTGGATCGAACGGGACCGC
ACCAGCGCGGGCTATCCGATCATGGTGCCAGTGGTCGATCTGGTCGAAATCGGCAACGGCGGCGGCTCCATCGCCTGGAC
CGACGACTTCGGCAAGCTGCATGTCGGCCCACAATCCGCCGGCGCATCGCCGGGGCCCGCCGCGTACGGCAACGGCGGCA
CCCGTGCCACGACGACCGATGCCAACCTCGTGCTCGGCCGCATCAACCGCGACTACTTCTGCGGCGGCGAAATCCAGGCC
GATATGGGCGCCGTCGATCGCGCGCTGCAGCCGCTGGCCGAGCGTCTGGGCCTGTCACGCGAGGACGTGGCCCGCGGCAT
CGTGCGCATCGCCAACGACAACATGGTCAATGCGCTCAAGCTGGTCTCGCTCAATCGCGGTCACGACCCGCGCGAGTTCA
CGCTGGTGGCATTCGGCGGCGGCGGTGCCATGCACGCGGTTGCACTGGGACAGGAGCTTGGCGTGAAGAAGGTGGTGATT
CCGCGCGGCGCATCGGTGTTCTCGGCGTGGGGCATGACCATGTCGGATCTGCGCCGCGACATGTTCGCCAACCGCTTCTT
CGAAGCGAGCCGCGACGGCGCGACAAGCTCGGCACAGGCTCTGCTCGAAGAACTCGCGCAGTCAGCACGGCAGCAGTACG
CACAGGAGGATATCGACCCGTCCCAGGTACGCGTGGCCCTGCATTGCAGGCTGCGCTACCAGAACCAGGAGCATGCGGTG
GAGGTGCAGTTGCTGCCCGGAGAGCGAGTCGATGCCGAGTGGGCGGCCATTGCCGAGCGCTTCCATGCGCTGTACGAGCA
GCAGTACACGTACCGGCTCGATGCCCCGCTTGAGATCGTCGGCTTCCATGTGGTCGCGGTCGCCGAGATCGGCAAGCTCG
ACCTGGATGAACTGCCGCTCAGCGGCGCAACGCTCGACGCAGCCATCAAAGGCAAGCGGCTCGTCGACTACGCGCTCGAA
GGCATCCACGAAGCCACCATCTACGACAGCGTGCACCTGGAACCAGGCATGGCCTTCGACGGCCCGGCGATTGTCGAAGA
CCCCGGCACGACCATCGTCGTGCATCCGGGCAACCGCGCCAGCGTCGACCGCCTCGGCAACCTCCACATCATCCTCTGA

Upstream 100 bases:

>100_bases
TTCCTAGAGTGCCTGCCAACGGCCGGAACCGAAAGAGTTCCGCCTGTCAGGCAACCGGCGCGGCAACGCCGCCGCGCCCG
AATTCTGCTAGGAGAAGCAA

Downstream 100 bases:

>100_bases
GGGCGAGGAAAACCATCATGAGCCGTACCGATATCTTCACGCTGGAAATCATCCAGAACTCGCTCCAGGCCATTGCCGAC
GAAATGTTCGCCGCCATGCG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MTQQRNTVRVATDVGGTFTDLVYFEFDPATGTQRIRTAKSDTTPPNFEQGILNVLRKADVDLEEVSFLAHGTTVVINALT
ERRGVRTGLITTEGFRDVLEIGRANRPDFFNLSWVKPSPFVPRYLRREVPGRIHFDGAERQSLDLSGVAQIIHDFRSEGV
EAVAICLMHAYANPTHEQAVLAEVRKLWPEASVVASHQITREWREYERTSTAVLSAYVQPIAARYLSNLEDGLRANGYRA
PLYVMQSNCGVDTVARAREIPITMVESGPASGFWGAAELGRLIGEPNVLALDIGGTTAKCSLIENGQVAIKTDYWIERDR
TSAGYPIMVPVVDLVEIGNGGGSIAWTDDFGKLHVGPQSAGASPGPAAYGNGGTRATTTDANLVLGRINRDYFCGGEIQA
DMGAVDRALQPLAERLGLSREDVARGIVRIANDNMVNALKLVSLNRGHDPREFTLVAFGGGGAMHAVALGQELGVKKVVI
PRGASVFSAWGMTMSDLRRDMFANRFFEASRDGATSSAQALLEELAQSARQQYAQEDIDPSQVRVALHCRLRYQNQEHAV
EVQLLPGERVDAEWAAIAERFHALYEQQYTYRLDAPLEIVGFHVVAVAEIGKLDLDELPLSGATLDAAIKGKRLVDYALE
GIHEATIYDSVHLEPGMAFDGPAIVEDPGTTIVVHPGNRASVDRLGNLHIIL

Sequences:

>Translated_692_residues
MTQQRNTVRVATDVGGTFTDLVYFEFDPATGTQRIRTAKSDTTPPNFEQGILNVLRKADVDLEEVSFLAHGTTVVINALT
ERRGVRTGLITTEGFRDVLEIGRANRPDFFNLSWVKPSPFVPRYLRREVPGRIHFDGAERQSLDLSGVAQIIHDFRSEGV
EAVAICLMHAYANPTHEQAVLAEVRKLWPEASVVASHQITREWREYERTSTAVLSAYVQPIAARYLSNLEDGLRANGYRA
PLYVMQSNCGVDTVARAREIPITMVESGPASGFWGAAELGRLIGEPNVLALDIGGTTAKCSLIENGQVAIKTDYWIERDR
TSAGYPIMVPVVDLVEIGNGGGSIAWTDDFGKLHVGPQSAGASPGPAAYGNGGTRATTTDANLVLGRINRDYFCGGEIQA
DMGAVDRALQPLAERLGLSREDVARGIVRIANDNMVNALKLVSLNRGHDPREFTLVAFGGGGAMHAVALGQELGVKKVVI
PRGASVFSAWGMTMSDLRRDMFANRFFEASRDGATSSAQALLEELAQSARQQYAQEDIDPSQVRVALHCRLRYQNQEHAV
EVQLLPGERVDAEWAAIAERFHALYEQQYTYRLDAPLEIVGFHVVAVAEIGKLDLDELPLSGATLDAAIKGKRLVDYALE
GIHEATIYDSVHLEPGMAFDGPAIVEDPGTTIVVHPGNRASVDRLGNLHIIL
>Mature_691_residues
TQQRNTVRVATDVGGTFTDLVYFEFDPATGTQRIRTAKSDTTPPNFEQGILNVLRKADVDLEEVSFLAHGTTVVINALTE
RRGVRTGLITTEGFRDVLEIGRANRPDFFNLSWVKPSPFVPRYLRREVPGRIHFDGAERQSLDLSGVAQIIHDFRSEGVE
AVAICLMHAYANPTHEQAVLAEVRKLWPEASVVASHQITREWREYERTSTAVLSAYVQPIAARYLSNLEDGLRANGYRAP
LYVMQSNCGVDTVARAREIPITMVESGPASGFWGAAELGRLIGEPNVLALDIGGTTAKCSLIENGQVAIKTDYWIERDRT
SAGYPIMVPVVDLVEIGNGGGSIAWTDDFGKLHVGPQSAGASPGPAAYGNGGTRATTTDANLVLGRINRDYFCGGEIQAD
MGAVDRALQPLAERLGLSREDVARGIVRIANDNMVNALKLVSLNRGHDPREFTLVAFGGGGAMHAVALGQELGVKKVVIP
RGASVFSAWGMTMSDLRRDMFANRFFEASRDGATSSAQALLEELAQSARQQYAQEDIDPSQVRVALHCRLRYQNQEHAVE
VQLLPGERVDAEWAAIAERFHALYEQQYTYRLDAPLEIVGFHVVAVAEIGKLDLDELPLSGATLDAAIKGKRLVDYALEG
IHEATIYDSVHLEPGMAFDGPAIVEDPGTTIVVHPGNRASVDRLGNLHIIL

Specific function: Unknown

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=752, Percent_Identity=29.3882978723404, Blast_Score=197, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI133901900, Length=738, Percent_Identity=24.2547425474255, Blast_Score=169, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322634, Length=763, Percent_Identity=25.5570117955439, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550492, Length=735, Percent_Identity=29.9319727891156, Blast_Score=244, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 75475; Mature: 75344

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQRNTVRVATDVGGTFTDLVYFEFDPATGTQRIRTAKSDTTPPNFEQGILNVLRKADV
CCCCCCEEEEEECCCCCEEEEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHCCC
DLEEVSFLAHGTTVVINALTERRGVRTGLITTEGFRDVLEIGRANRPDFFNLSWVKPSPF
CHHHHHHHHCCHHHEEEHHHHHCCCEEEEEECHHHHHHHHHCCCCCCCEEEEEECCCCCC
VPRYLRREVPGRIHFDGAERQSLDLSGVAQIIHDFRSEGVEAVAICLMHAYANPTHEQAV
CHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
LAEVRKLWPEASVVASHQITREWREYERTSTAVLSAYVQPIAARYLSNLEDGLRANGYRA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLYVMQSNCGVDTVARAREIPITMVESGPASGFWGAAELGRLIGEPNVLALDIGGTTAKC
CEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEE
SLIENGQVAIKTDYWIERDRTSAGYPIMVPVVDLVEIGNGGGSIAWTDDFGKLHVGPQSA
EEEECCCEEEEECCEEECCCCCCCCEEEEEHHHHHEECCCCCCEEEECCCCEEEECCCCC
GASPGPAAYGNGGTRATTTDANLVLGRINRDYFCGGEIQADMGAVDRALQPLAERLGLSR
CCCCCCCCCCCCCCEEEECCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCH
EDVARGIVRIANDNMVNALKLVSLNRGHDPREFTLVAFGGGGAMHAVALGQELGVKKVVI
HHHHHHHHEECCCCHHHHEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEE
PRGASVFSAWGMTMSDLRRDMFANRFFEASRDGATSSAQALLEELAQSARQQYAQEDIDP
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
SQVRVALHCRLRYQNQEHAVEVQLLPGERVDAEWAAIAERFHALYEQQYTYRLDAPLEIV
HHEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHH
GFHVVAVAEIGKLDLDELPLSGATLDAAIKGKRLVDYALEGIHEATIYDSVHLEPGMAFD
HHEEEEEHHHCCCCHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHEEECEECCCCCCCC
GPAIVEDPGTTIVVHPGNRASVDRLGNLHIIL
CCEEEECCCCEEEEECCCCCCHHHCCCEEEEC
>Mature Secondary Structure 
TQQRNTVRVATDVGGTFTDLVYFEFDPATGTQRIRTAKSDTTPPNFEQGILNVLRKADV
CCCCCEEEEEECCCCCEEEEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHCCC
DLEEVSFLAHGTTVVINALTERRGVRTGLITTEGFRDVLEIGRANRPDFFNLSWVKPSPF
CHHHHHHHHCCHHHEEEHHHHHCCCEEEEEECHHHHHHHHHCCCCCCCEEEEEECCCCCC
VPRYLRREVPGRIHFDGAERQSLDLSGVAQIIHDFRSEGVEAVAICLMHAYANPTHEQAV
CHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
LAEVRKLWPEASVVASHQITREWREYERTSTAVLSAYVQPIAARYLSNLEDGLRANGYRA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLYVMQSNCGVDTVARAREIPITMVESGPASGFWGAAELGRLIGEPNVLALDIGGTTAKC
CEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEE
SLIENGQVAIKTDYWIERDRTSAGYPIMVPVVDLVEIGNGGGSIAWTDDFGKLHVGPQSA
EEEECCCEEEEECCEEECCCCCCCCEEEEEHHHHHEECCCCCCEEEECCCCEEEECCCCC
GASPGPAAYGNGGTRATTTDANLVLGRINRDYFCGGEIQADMGAVDRALQPLAERLGLSR
CCCCCCCCCCCCCCEEEECCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCH
EDVARGIVRIANDNMVNALKLVSLNRGHDPREFTLVAFGGGGAMHAVALGQELGVKKVVI
HHHHHHHHEECCCCHHHHEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEE
PRGASVFSAWGMTMSDLRRDMFANRFFEASRDGATSSAQALLEELAQSARQQYAQEDIDP
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
SQVRVALHCRLRYQNQEHAVEVQLLPGERVDAEWAAIAERFHALYEQQYTYRLDAPLEIV
HHEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHH
GFHVVAVAEIGKLDLDELPLSGATLDAAIKGKRLVDYALEGIHEATIYDSVHLEPGMAFD
HHEEEEEHHHCCCCHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHEEECEECCCCCCCC
GPAIVEDPGTTIVVHPGNRASVDRLGNLHIIL
CCEEEECCCCEEEEECCCCCCHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]