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The map label for this gene is 73539295

Identifier: 73539295

GI number: 73539295

Start: 2265019

End: 2266677

Strand: Reverse

Name: 73539295

Synonym: Reut_B5473

Alternate gene names: NA

Gene position: 2266677-2265019 (Counterclockwise)

Preceding gene: 73539296

Following gene: 73539294

Centisome position: 83.15

GC content: 67.09

Gene sequence:

>1659_bases
ATGAGCCGTACCGATATCTTCACGCTGGAAATCATCCAGAACTCGCTCCAGGCCATTGCCGACGAAATGTTCGCCGCCAT
GCGCAAGACCGCGATGAGCCCGATCATCTACGAGGTGCTCGACATGGGCACGGGCATCACTGATGCGCAGGGACAGATGG
CGAGCTCGGGCTCCGGCATTCCGGCCTTCGTCGGCGTGCTGGACAAGGCCGTGCGCTGCGTGCTGGCGCAGTATTCGCAA
CCCGGTGACATCGAGCCGGGCGACATCTTCATCACGAACGATCCGCACACCGGCGGTGTCACGCACCTGAACGACGTCGT
GCTGACCATGCCGGTGTTCGCCGGCGACCGGCTGGTCGCATGGACCGCCAATATCGCGCACTGGAACGACGTGGGCGGCA
TGGTGCCCGGATCGCTGAGCACCGATGCGACCGACATCTTCCAGGAAGGACTGCGCCTGACCGGCGTCAAGCTGATCTCC
GGCGGCAAGCCAGTGCGTCCGGTCATGCAGATCCTGCGCGCCAACACGCGCATGCCTGACTTCATGGAAGGCGACCTGTG
GGCCGGCATTGCGGCGGTGCGCGGCGGCGCGGGACGGATCGTCGAGCTGACGCGCAAGTACGGTGTCGAGGCCTTCGTCG
CGGCAATAGGCGACTTCATGGAGCATGGCGAGCGCGTCTCGCTCCATGCGCTTGCCGACGCGCCGCGCGGACGCTTCACG
ATGGAAGAACTGCAGGACGACGGCCAGACGTTCCGCGTGACGGTCGAACTCTCCGGCAACGAGATGATCGTCGATCTGTG
CGACAACCCCGCCCAGGCGTGCGGCCCTACGAACCTGAGCCGCGACGGCACCATCGTGGCGACGCAGATGGCGTTCAAGG
CCGTCACTGCGCCGCAGGCCGCGACCAATGGTGGCACGTTCCGTCCGCTGAAGGTGCTGACGAAGCCCGGCACGCTGTTC
GACGCGAGCATGCCGGCCGCGGAGGGCTTCTATTTCGAGAACCTGATCCGCGTCTATGACCTGATCCTGCGCTGCCTTGC
CGCGCACATGCCGGAACGCCTGCCCGCAGGCAATTTTGCATCGGTCTGCGCGACCATCATCGGTGGCGTGCATCCGGACA
CCGGCCGCGCCTTTACGCTGGTCGAGCCCGAGATCGGCGGCTGGGGCGGCGAGGCCGGCCGCGACGGCAACAGCGCGATG
TACTCGGGCCTCCATGGCGAGACCTACAACTGCCCCGCTGAAATCTGCGAGGCCCGCTACGGGCTGTATGTCGATGGCAT
GGCCCTCAACGATGAGCCGGGCGGCCACGGCGAGTACCGCGGCGGACTCGGCATCCGCATCGACTACCGCGTGCGCGCCG
ACGACACCTTCCTGACCTGCGGCTACACGCGCTCGCAAGTCCCGCCGTGGGGCCTTGCAGGCGGCCGCGACGGCACGCCG
AACTATATCGAGGTGCTGCGCAAGGATGGCCGCAGCGAGCGGCACGCCATGGTCAGCGGCCTGATGCTGCAACAGGGCGA
CGTCATCCGCGTGCGTACCGGTGCGGGCGGCGGCTACGGCGCCCCTGCCAGTCGTTCGCCCGAGGCCGTGCGCGACGACG
TGCGCAACGGCTACCTGAGCGCGGCGCAGGCCCGTGAAATCTACGGCGTCACGGTCTGA

Upstream 100 bases:

>100_bases
AAGACCCCGGCACGACCATCGTCGTGCATCCGGGCAACCGCGCCAGCGTCGACCGCCTCGGCAACCTCCACATCATCCTC
TGAGGGCGAGGAAAACCATC

Downstream 100 bases:

>100_bases
CATGGGCTGCGCCATCGAACCTATCGGCGTTCCCATGTTTGACAAGGGCGTCTACCGGGCACGCCGTCGCCAGCTGCGCG
AGCGCGTCGGCACGGGTCTG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSRTDIFTLEIIQNSLQAIADEMFAAMRKTAMSPIIYEVLDMGTGITDAQGQMASSGSGIPAFVGVLDKAVRCVLAQYSQ
PGDIEPGDIFITNDPHTGGVTHLNDVVLTMPVFAGDRLVAWTANIAHWNDVGGMVPGSLSTDATDIFQEGLRLTGVKLIS
GGKPVRPVMQILRANTRMPDFMEGDLWAGIAAVRGGAGRIVELTRKYGVEAFVAAIGDFMEHGERVSLHALADAPRGRFT
MEELQDDGQTFRVTVELSGNEMIVDLCDNPAQACGPTNLSRDGTIVATQMAFKAVTAPQAATNGGTFRPLKVLTKPGTLF
DASMPAAEGFYFENLIRVYDLILRCLAAHMPERLPAGNFASVCATIIGGVHPDTGRAFTLVEPEIGGWGGEAGRDGNSAM
YSGLHGETYNCPAEICEARYGLYVDGMALNDEPGGHGEYRGGLGIRIDYRVRADDTFLTCGYTRSQVPPWGLAGGRDGTP
NYIEVLRKDGRSERHAMVSGLMLQQGDVIRVRTGAGGGYGAPASRSPEAVRDDVRNGYLSAAQAREIYGVTV

Sequences:

>Translated_552_residues
MSRTDIFTLEIIQNSLQAIADEMFAAMRKTAMSPIIYEVLDMGTGITDAQGQMASSGSGIPAFVGVLDKAVRCVLAQYSQ
PGDIEPGDIFITNDPHTGGVTHLNDVVLTMPVFAGDRLVAWTANIAHWNDVGGMVPGSLSTDATDIFQEGLRLTGVKLIS
GGKPVRPVMQILRANTRMPDFMEGDLWAGIAAVRGGAGRIVELTRKYGVEAFVAAIGDFMEHGERVSLHALADAPRGRFT
MEELQDDGQTFRVTVELSGNEMIVDLCDNPAQACGPTNLSRDGTIVATQMAFKAVTAPQAATNGGTFRPLKVLTKPGTLF
DASMPAAEGFYFENLIRVYDLILRCLAAHMPERLPAGNFASVCATIIGGVHPDTGRAFTLVEPEIGGWGGEAGRDGNSAM
YSGLHGETYNCPAEICEARYGLYVDGMALNDEPGGHGEYRGGLGIRIDYRVRADDTFLTCGYTRSQVPPWGLAGGRDGTP
NYIEVLRKDGRSERHAMVSGLMLQQGDVIRVRTGAGGGYGAPASRSPEAVRDDVRNGYLSAAQAREIYGVTV
>Mature_551_residues
SRTDIFTLEIIQNSLQAIADEMFAAMRKTAMSPIIYEVLDMGTGITDAQGQMASSGSGIPAFVGVLDKAVRCVLAQYSQP
GDIEPGDIFITNDPHTGGVTHLNDVVLTMPVFAGDRLVAWTANIAHWNDVGGMVPGSLSTDATDIFQEGLRLTGVKLISG
GKPVRPVMQILRANTRMPDFMEGDLWAGIAAVRGGAGRIVELTRKYGVEAFVAAIGDFMEHGERVSLHALADAPRGRFTM
EELQDDGQTFRVTVELSGNEMIVDLCDNPAQACGPTNLSRDGTIVATQMAFKAVTAPQAATNGGTFRPLKVLTKPGTLFD
ASMPAAEGFYFENLIRVYDLILRCLAAHMPERLPAGNFASVCATIIGGVHPDTGRAFTLVEPEIGGWGGEAGRDGNSAMY
SGLHGETYNCPAEICEARYGLYVDGMALNDEPGGHGEYRGGLGIRIDYRVRADDTFLTCGYTRSQVPPWGLAGGRDGTPN
YIEVLRKDGRSERHAMVSGLMLQQGDVIRVRTGAGGGYGAPASRSPEAVRDDVRNGYLSAAQAREIYGVTV

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=542, Percent_Identity=27.6752767527675, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI133901900, Length=551, Percent_Identity=25.7713248638838, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI133901902, Length=515, Percent_Identity=25.0485436893204, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322634, Length=550, Percent_Identity=24.7272727272727, Blast_Score=133, Evalue=8e-32,
Organism=Drosophila melanogaster, GI45550492, Length=566, Percent_Identity=26.1484098939929, Blast_Score=138, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 58980; Mature: 58848

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTDIFTLEIIQNSLQAIADEMFAAMRKTAMSPIIYEVLDMGTGITDAQGQMASSGSGI
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAFVGVLDKAVRCVLAQYSQPGDIEPGDIFITNDPHTGGVTHLNDVVLTMPVFAGDRLVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHCCEEEEEEEECCCEEEE
WTANIAHWNDVGGMVPGSLSTDATDIFQEGLRLTGVKLISGGKPVRPVMQILRANTRMPD
EEECCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHHCCCCCC
FMEGDLWAGIAAVRGGAGRIVELTRKYGVEAFVAAIGDFMEHGERVSLHALADAPRGRFT
CCCCHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
MEELQDDGQTFRVTVELSGNEMIVDLCDNPAQACGPTNLSRDGTIVATQMAFKAVTAPQA
HHHHHCCCCEEEEEEEECCCEEEEEECCCCHHHCCCCCCCCCCCEEEHHHHHHHHCCCCC
ATNGGTFRPLKVLTKPGTLFDASMPAAEGFYFENLIRVYDLILRCLAAHMPERLPAGNFA
CCCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SVCATIIGGVHPDTGRAFTLVEPEIGGWGGEAGRDGNSAMYSGLHGETYNCPAEICEARY
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHC
GLYVDGMALNDEPGGHGEYRGGLGIRIDYRVRADDTFLTCGYTRSQVPPWGLAGGRDGTP
CEEECCEEECCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCH
NYIEVLRKDGRSERHAMVSGLMLQQGDVIRVRTGAGGGYGAPASRSPEAVRDDVRNGYLS
HHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHH
AAQAREIYGVTV
HHHHHHEEEECC
>Mature Secondary Structure 
SRTDIFTLEIIQNSLQAIADEMFAAMRKTAMSPIIYEVLDMGTGITDAQGQMASSGSGI
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAFVGVLDKAVRCVLAQYSQPGDIEPGDIFITNDPHTGGVTHLNDVVLTMPVFAGDRLVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHHCCEEEEEEEECCCEEEE
WTANIAHWNDVGGMVPGSLSTDATDIFQEGLRLTGVKLISGGKPVRPVMQILRANTRMPD
EEECCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHHCCCCCC
FMEGDLWAGIAAVRGGAGRIVELTRKYGVEAFVAAIGDFMEHGERVSLHALADAPRGRFT
CCCCHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
MEELQDDGQTFRVTVELSGNEMIVDLCDNPAQACGPTNLSRDGTIVATQMAFKAVTAPQA
HHHHHCCCCEEEEEEEECCCEEEEEECCCCHHHCCCCCCCCCCCEEEHHHHHHHHCCCCC
ATNGGTFRPLKVLTKPGTLFDASMPAAEGFYFENLIRVYDLILRCLAAHMPERLPAGNFA
CCCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SVCATIIGGVHPDTGRAFTLVEPEIGGWGGEAGRDGNSAMYSGLHGETYNCPAEICEARY
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHC
GLYVDGMALNDEPGGHGEYRGGLGIRIDYRVRADDTFLTCGYTRSQVPPWGLAGGRDGTP
CEEECCEEECCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCH
NYIEVLRKDGRSERHAMVSGLMLQQGDVIRVRTGAGGGYGAPASRSPEAVRDDVRNGYLS
HHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHH
AAQAREIYGVTV
HHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]