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The map label for this gene is mscS [H]

Identifier: 73539153

GI number: 73539153

Start: 2097550

End: 2098455

Strand: Reverse

Name: mscS [H]

Synonym: Reut_B5331

Alternate gene names: 73539153

Gene position: 2098455-2097550 (Counterclockwise)

Preceding gene: 73539154

Following gene: 73539151

Centisome position: 76.97

GC content: 66.0

Gene sequence:

>906_bases
ATGAACTGGAATATGGACGCCGTGAAGCTCGATACCGCATGGAACTACCTGGTGCAGCTGGCCGTGCATCAAGGCATGAA
CTGCCTGGTCGCCGTACTGATCCTGATCGCGGGATGGTGGTTGTCGGCGTGTGCGGCCGAATCGGCGCAGCGCGCGCTGG
ACCGCACGCACCTGGATGAAACCGTGCGGCCGATGCTGGCCAACACGTTCCAGTGGATGGTGCGCGTGCTTACCATCGTG
CTGGTGCTGTCCCAGTTCGGGGTGCAGACGGCCAGCATCATCGCCATGCTCGGCGCGGCCGGGCTTGCGATCGGGCTGGC
GCTGCAGGGAACGCTGCAGAACATTGCAGCCGGCCTCATGCTGGTGCTGCTGCGGCCGTTCCGCGTCGGGCAGTACATCG
ATGCTCAAGGGGTTGCTGGCACGGTCCGCGAGGTCAGCCTGTTCATGACCGAGCTGACCACGGCTGACGGTGTCTGCCTG
CGCGTGCCCAATGGCAAGCTGTGGGGCAGCGCGATCACGAACTACAGCGAGAACGCGTCGCGCCGCATCGACATCGAGAC
CACGGTGGCGTTCGGCGGTGACATGCACAAGGGCCTGGACGCACTGCACATGATGCTGTCCGCCGAGCCGCGCCTGTTGC
CGGAGCCGAAGCCCGAGGCGATGGTCGTCAACTACACCGAGCAGGGCGTCACGCTGAACCTGCGCTGCTGGACTTCGAGC
GAGGACTACTGGACGGTGCGCTACGCGTTCTACGAGCGCATCAAGAAGGTGCTCGAGGACGCGGGCTGCAAGCTCGCATC
ACCGATCCAGGAGGTGCGCATGCCAGACCCGGGCGCCACGCCGGTCACCACACCGCCGACCGCAACGCCCGATCCGCGCG
CCAATCCGTTGCGGCGCGCGGGCTAG

Upstream 100 bases:

>100_bases
CAACGCGCCTGATCCGTTGCTTCACATTGCGCCTTGTGTCGCATAACGGCCTGGCATACGCGCGCAGAAACGGGTACGCC
CCCACCTGGAGCCGCTTCTC

Downstream 100 bases:

>100_bases
TTCGCTGAGTCAGTCGCGCGCGTCTTCGTCGGCGAACAGCGTGCAGGCGCCCGCTTCGGCATCACTGACGTTGCGGCGGA
AGGTCGCGAACAGGTCGCGT

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNWNMDAVKLDTAWNYLVQLAVHQGMNCLVAVLILIAGWWLSACAAESAQRALDRTHLDETVRPMLANTFQWMVRVLTIV
LVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGLMLVLLRPFRVGQYIDAQGVAGTVREVSLFMTELTTADGVCL
RVPNGKLWGSAITNYSENASRRIDIETTVAFGGDMHKGLDALHMMLSAEPRLLPEPKPEAMVVNYTEQGVTLNLRCWTSS
EDYWTVRYAFYERIKKVLEDAGCKLASPIQEVRMPDPGATPVTTPPTATPDPRANPLRRAG

Sequences:

>Translated_301_residues
MNWNMDAVKLDTAWNYLVQLAVHQGMNCLVAVLILIAGWWLSACAAESAQRALDRTHLDETVRPMLANTFQWMVRVLTIV
LVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGLMLVLLRPFRVGQYIDAQGVAGTVREVSLFMTELTTADGVCL
RVPNGKLWGSAITNYSENASRRIDIETTVAFGGDMHKGLDALHMMLSAEPRLLPEPKPEAMVVNYTEQGVTLNLRCWTSS
EDYWTVRYAFYERIKKVLEDAGCKLASPIQEVRMPDPGATPVTTPPTATPDPRANPLRRAG
>Mature_301_residues
MNWNMDAVKLDTAWNYLVQLAVHQGMNCLVAVLILIAGWWLSACAAESAQRALDRTHLDETVRPMLANTFQWMVRVLTIV
LVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGLMLVLLRPFRVGQYIDAQGVAGTVREVSLFMTELTTADGVCL
RVPNGKLWGSAITNYSENASRRIDIETTVAFGGDMHKGLDALHMMLSAEPRLLPEPKPEAMVVNYTEQGVTLNLRCWTSS
EDYWTVRYAFYERIKKVLEDAGCKLASPIQEVRMPDPGATPVTTPPTATPDPRANPLRRAG

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=259, Percent_Identity=36.6795366795367, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787591, Length=235, Percent_Identity=23.4042553191489, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786670, Length=157, Percent_Identity=25.4777070063694, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32955; Mature: 32955

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWNMDAVKLDTAWNYLVQLAVHQGMNCLVAVLILIAGWWLSACAAESAQRALDRTHLDE
CCCCCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVRPMLANTFQWMVRVLTIVLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLRPFRVGQYIDAQGVAGTVREVSLFMTELTTADGVCLRVPNGKLWGSAITNYSENAS
HHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHCCCCCCC
RRIDIETTVAFGGDMHKGLDALHMMLSAEPRLLPEPKPEAMVVNYTEQGVTLNLRCWTSS
CEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEECC
EDYWTVRYAFYERIKKVLEDAGCKLASPIQEVRMPDPGATPVTTPPTATPDPRANPLRRA
CCCEEHHHHHHHHHHHHHHHCCCCHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MNWNMDAVKLDTAWNYLVQLAVHQGMNCLVAVLILIAGWWLSACAAESAQRALDRTHLDE
CCCCCCEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVRPMLANTFQWMVRVLTIVLVLSQFGVQTASIIAMLGAAGLAIGLALQGTLQNIAAGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLLRPFRVGQYIDAQGVAGTVREVSLFMTELTTADGVCLRVPNGKLWGSAITNYSENAS
HHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHCCCCCCC
RRIDIETTVAFGGDMHKGLDALHMMLSAEPRLLPEPKPEAMVVNYTEQGVTLNLRCWTSS
CEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEECC
EDYWTVRYAFYERIKKVLEDAGCKLASPIQEVRMPDPGATPVTTPPTATPDPRANPLRRA
CCCEEHHHHHHHHHHHHHHHCCCCHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]