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The map label for this gene is glk

Identifier: 73539149

GI number: 73539149

Start: 2092277

End: 2093308

Strand: Reverse

Name: glk

Synonym: Reut_B5327

Alternate gene names: 73539149

Gene position: 2093308-2092277 (Counterclockwise)

Preceding gene: 73539150

Following gene: 73539148

Centisome position: 76.79

GC content: 68.7

Gene sequence:

>1032_bases
ATGAGCAACGCCATGGCTGCTTCCGCCGTGCCCGATGACACCGCCTATCCGCGTTTGCTGGCCGACGTGGGCGGGACCAA
TGTGCGCTTCGCACTGGAAACGGCGCCGATGCAGATCGGCGCCGTGACCGCGCTGAAGGTGGCCGACCATCCGTCGCTGG
AAGCGGCCATGCGCCACTACCGCGATGCGCTGTCTGCCTCGGGTGCCAAGTTGCCGCGCCATGCTGCCATCGGTCTCGCG
AATCCGGTCACGGGCGACCACGTTCGCCTGACCAACCACGACTGGGCCTTCTCGATCGAAGCCACGCGGCAAGCGCTCGG
GCTGCAGACGCTCGTGGCCATCAACGATTTCACGTCGCTGGCGCTGGGCTTGCCTTACCTTGGCGCGAACGATCTCGTGC
AGATCCGCTCCGGCCAAGCCGTGGCCACTGCGCCGCGTGCGCTGATCGGGCCTGGCACCGGGTTGGGCGTGTCGGGGCTG
GTGCCCGCGCCGGGCGGTGGCGCCGTGGCGCTGGCGGGGGAGGGCGGCCATATCGAACTCATGCCCGTGACCGACGACGA
GTGGATCGCCTGGCGCGCCACGCACGCGAGCCTTGGGCGGGTGTCGGCGGAGCGGTTGTTGTCAGGCATGGGGCTGTCGC
AGATCCATGCCGCGCTGTCCGCAGAAACGGGTACGCGCGTGGACGTGCCGCTGACGCCCGAGCAGGTCACGACGGGTGCC
TTCGCACGGCACGATCCGCTCTGCGAGCGCACCATGGCGGTGTTTTTTGGCCTGCTCGGATCTGTCGCAGCGGACATCGC
GCTGGTAATGGGCGCGCTTGGCGGCGTGTATCTCGGTGGCGGCATCCTGCCGCGCTTCGTGCCAGCGCTGCAGGCGTCGG
CCTTCAATGCGCGCTTCGTCGCCAAGGGCCGCATGCGCGGCTATCTCGACAAGCTGCCTGTCTACGTGATTACCGCGAGC
TATCCCGCGCTGCCGGGCCTGGCGCGCGCGCTGGCCGATACGCTCAGTCACGGCCGCCCACATATCGGATAA

Upstream 100 bases:

>100_bases
AGGCCGAGGTGCTGGCCCGCGCAATGCAAGGCCCGACGCCGGCGCTGCCGGTCAGCATCGTGCTGGCCAACCAGCGCCGC
GGCTTCGACCTTTTCCGTGC

Downstream 100 bases:

>100_bases
GACATGCGCGTGCTGCTGGTGGAAGACGATGCCATGATCGGCGACAGCGTGAAGCTTGCGTTGCGCCAGGAAGGCTTTGC
GGTGGACTGGGTGCAGGATG

Product: glucokinase

Products: NA

Alternate protein names: Glucose kinase

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSNAMAASAVPDDTAYPRLLADVGGTNVRFALETAPMQIGAVTALKVADHPSLEAAMRHYRDALSASGAKLPRHAAIGLA
NPVTGDHVRLTNHDWAFSIEATRQALGLQTLVAINDFTSLALGLPYLGANDLVQIRSGQAVATAPRALIGPGTGLGVSGL
VPAPGGGAVALAGEGGHIELMPVTDDEWIAWRATHASLGRVSAERLLSGMGLSQIHAALSAETGTRVDVPLTPEQVTTGA
FARHDPLCERTMAVFFGLLGSVAADIALVMGALGGVYLGGGILPRFVPALQASAFNARFVAKGRMRGYLDKLPVYVITAS
YPALPGLARALADTLSHGRPHIG

Sequences:

>Translated_343_residues
MSNAMAASAVPDDTAYPRLLADVGGTNVRFALETAPMQIGAVTALKVADHPSLEAAMRHYRDALSASGAKLPRHAAIGLA
NPVTGDHVRLTNHDWAFSIEATRQALGLQTLVAINDFTSLALGLPYLGANDLVQIRSGQAVATAPRALIGPGTGLGVSGL
VPAPGGGAVALAGEGGHIELMPVTDDEWIAWRATHASLGRVSAERLLSGMGLSQIHAALSAETGTRVDVPLTPEQVTTGA
FARHDPLCERTMAVFFGLLGSVAADIALVMGALGGVYLGGGILPRFVPALQASAFNARFVAKGRMRGYLDKLPVYVITAS
YPALPGLARALADTLSHGRPHIG
>Mature_342_residues
SNAMAASAVPDDTAYPRLLADVGGTNVRFALETAPMQIGAVTALKVADHPSLEAAMRHYRDALSASGAKLPRHAAIGLAN
PVTGDHVRLTNHDWAFSIEATRQALGLQTLVAINDFTSLALGLPYLGANDLVQIRSGQAVATAPRALIGPGTGLGVSGLV
PAPGGGAVALAGEGGHIELMPVTDDEWIAWRATHASLGRVSAERLLSGMGLSQIHAALSAETGTRVDVPLTPEQVTTGAF
ARHDPLCERTMAVFFGLLGSVAADIALVMGALGGVYLGGGILPRFVPALQASAFNARFVAKGRMRGYLDKLPVYVITASY
PALPGLARALADTLSHGRPHIG

Specific function: Not Highly Important In E.Coli As Glucose Is Transported Into The Cell By The Pts System Already As Glucose 6-Phosphate. [C]

COG id: COG0837

COG function: function code G; Glucokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial glucokinase family

Homologues:

Organism=Escherichia coli, GI1788732, Length=320, Percent_Identity=38.75, Blast_Score=215, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLK_CUPPJ (Q46QB2)

Other databases:

- EMBL:   CP000091
- RefSeq:   YP_299516.1
- ProteinModelPortal:   Q46QB2
- GeneID:   3612765
- GenomeReviews:   CP000091_GR
- KEGG:   reu:Reut_B5327
- NMPDR:   fig|264198.3.peg.4854
- HOGENOM:   HBG644410
- OMA:   VDIGGTH
- ProtClustDB:   CLSK769004
- BioCyc:   REUT264198:REUT_B5327-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00524
- InterPro:   IPR003836
- TIGRFAMs:   TIGR00749

Pfam domain/function: PF02685 Glucokinase

EC number: =2.7.1.2

Molecular weight: Translated: 35393; Mature: 35262

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAMAASAVPDDTAYPRLLADVGGTNVRFALETAPMQIGAVTALKVADHPSLEAAMRHY
CCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEECCEEECCEEEEEECCCCCHHHHHHHH
RDALSASGAKLPRHAAIGLANPVTGDHVRLTNHDWAFSIEATRQALGLQTLVAINDFTSL
HHHHHCCCCCCCCHHHCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHH
ALGLPYLGANDLVQIRSGQAVATAPRALIGPGTGLGVSGLVPAPGGGAVALAGEGGHIEL
HHCCCCCCCCCEEEEECCCEEECCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
MPVTDDEWIAWRATHASLGRVSAERLLSGMGLSQIHAALSAETGTRVDVPLTPEQVTTGA
EECCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCC
FARHDPLCERTMAVFFGLLGSVAADIALVMGALGGVYLGGGILPRFVPALQASAFNARFV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEE
AKGRMRGYLDKLPVYVITASYPALPGLARALADTLSHGRPHIG
ECCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNAMAASAVPDDTAYPRLLADVGGTNVRFALETAPMQIGAVTALKVADHPSLEAAMRHY
CCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEECCEEECCEEEEEECCCCCHHHHHHHH
RDALSASGAKLPRHAAIGLANPVTGDHVRLTNHDWAFSIEATRQALGLQTLVAINDFTSL
HHHHHCCCCCCCCHHHCCCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHH
ALGLPYLGANDLVQIRSGQAVATAPRALIGPGTGLGVSGLVPAPGGGAVALAGEGGHIEL
HHCCCCCCCCCEEEEECCCEEECCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
MPVTDDEWIAWRATHASLGRVSAERLLSGMGLSQIHAALSAETGTRVDVPLTPEQVTTGA
EECCCCCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCC
FARHDPLCERTMAVFFGLLGSVAADIALVMGALGGVYLGGGILPRFVPALQASAFNARFV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEE
AKGRMRGYLDKLPVYVITASYPALPGLARALADTLSHGRPHIG
ECCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA