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The map label for this gene is pcaR [H]

Identifier: 73539134

GI number: 73539134

Start: 2075095

End: 2075943

Strand: Direct

Name: pcaR [H]

Synonym: Reut_B5311

Alternate gene names: 73539134

Gene position: 2075095-2075943 (Clockwise)

Preceding gene: 73539133

Following gene: 73539139

Centisome position: 76.12

GC content: 67.14

Gene sequence:

>849_bases
ATGGAGACCACCCGCCCCCTTGGTATCGGCCAGGCCCAGGCCGATACCGTCCGCGTTGCCTATCCCCAGTCTGATCTGCT
CAGCAGTTTCTCCGGCGACCCGAACTTCATGCTGTCGCTGGCACGCGGCCTGACCGTGCTGGAGGCGTTTTCCGAGCGCA
AGCGCCCGCTGACGATCTCGCAAGTGGCGCAGCGCACGCAGTTGTCGCGCGCGTCGGTGCGCCGCTGCCTGTACACGCTG
GAGCAGCTCGGCTACGTCAGCCAGCAGGACGGGCAGTTCGCGCTGCGCCCGCGCGTGCTGCACCTCGGGCACGCGTATTT
CTCGTCGACGTCGATGGTTTCGCTGTCACAGCCGATCCTGGACAACCTGAGCGCGCGCATCCACGAGACCTGCACGCTGG
CCATCCTGGACGGCACCGACATCCTCTATCTCGTGCGTTCGGAAGTGCAGCGCGTGGTCAATTACTCGCTCGGCATGGGC
AGCCGGCTACCGGCCTATTGCACGTCGAACGGGCGCCTGCTGCTGGCGCACCAGCCGGCGTCGGTGCTCGACGGTTTCTT
CGAGCATGCCGACCTGCGGCCGCGCACGCTACAAACCAAGGTATCGCGCCAGGAACTCGAAGCATGCTTCGAGCGCGCCC
GCGAACTCGACTACGTGGTCGTCGATGAAGAACTCGAACCGGGCCTGCGCGCCATGGCGGTGCCGGTGCGTTCGGCCGCG
GGCATCGTGCTGGCCGGCATCAGCGTGAGCGTGCGTGCGGCCAAGGTGACGGAAGCGGAAATGGTGTCGCGCCTGCTGCC
GCCGATGCGCGAAGCGGCCGCGGCCATTGGCCGCCTGATCACGTCCTGA

Upstream 100 bases:

>100_bases
CGGGTGTACAGGGCGTCTTTGCCCCAGTGCTCCAAAACCACTGGCCCGCGGCGCCGACTCCGTTTAAGATTGCGCGATTA
TCGAACAAAGCAAATCAAAG

Downstream 100 bases:

>100_bases
TCGTACGTCCTGACCGCGCCGCGGTTCGCTGAGGCAGCTTGCGGCCTCAGCCGCGGTGCGCGAGTTCCAGCGCAAACACC
AGGCGCTGCAGAAAATTCTC

Product: regulatory protein IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
METTRPLGIGQAQADTVRVAYPQSDLLSSFSGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTL
EQLGYVSQQDGQFALRPRVLHLGHAYFSSTSMVSLSQPILDNLSARIHETCTLAILDGTDILYLVRSEVQRVVNYSLGMG
SRLPAYCTSNGRLLLAHQPASVLDGFFEHADLRPRTLQTKVSRQELEACFERARELDYVVVDEELEPGLRAMAVPVRSAA
GIVLAGISVSVRAAKVTEAEMVSRLLPPMREAAAAIGRLITS

Sequences:

>Translated_282_residues
METTRPLGIGQAQADTVRVAYPQSDLLSSFSGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTL
EQLGYVSQQDGQFALRPRVLHLGHAYFSSTSMVSLSQPILDNLSARIHETCTLAILDGTDILYLVRSEVQRVVNYSLGMG
SRLPAYCTSNGRLLLAHQPASVLDGFFEHADLRPRTLQTKVSRQELEACFERARELDYVVVDEELEPGLRAMAVPVRSAA
GIVLAGISVSVRAAKVTEAEMVSRLLPPMREAAAAIGRLITS
>Mature_282_residues
METTRPLGIGQAQADTVRVAYPQSDLLSSFSGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTL
EQLGYVSQQDGQFALRPRVLHLGHAYFSSTSMVSLSQPILDNLSARIHETCTLAILDGTDILYLVRSEVQRVVNYSLGMG
SRLPAYCTSNGRLLLAHQPASVLDGFFEHADLRPRTLQTKVSRQELEACFERARELDYVVVDEELEPGLRAMAVPVRSAA
GIVLAGISVSVRAAKVTEAEMVSRLLPPMREAAAAIGRLITS

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=243, Percent_Identity=29.6296296296296, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788131, Length=249, Percent_Identity=28.5140562248996, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082359, Length=224, Percent_Identity=27.6785714285714, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786715, Length=228, Percent_Identity=27.6315789473684, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789999, Length=249, Percent_Identity=25.7028112449799, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30975; Mature: 30975

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTRPLGIGQAQADTVRVAYPQSDLLSSFSGDPNFMLSLARGLTVLEAFSERKRPLTIS
CCCCCCCCCCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVAQRTQLSRASVRRCLYTLEQLGYVSQQDGQFALRPRVLHLGHAYFSSTSMVSLSQPIL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHH
DNLSARIHETCTLAILDGTDILYLVRSEVQRVVNYSLGMGSRLPAYCTSNGRLLLAHQPA
HHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCH
SVLDGFFEHADLRPRTLQTKVSRQELEACFERARELDYVVVDEELEPGLRAMAVPVRSAA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCC
GIVLAGISVSVRAAKVTEAEMVSRLLPPMREAAAAIGRLITS
CEEEECCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
METTRPLGIGQAQADTVRVAYPQSDLLSSFSGDPNFMLSLARGLTVLEAFSERKRPLTIS
CCCCCCCCCCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVAQRTQLSRASVRRCLYTLEQLGYVSQQDGQFALRPRVLHLGHAYFSSTSMVSLSQPIL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHH
DNLSARIHETCTLAILDGTDILYLVRSEVQRVVNYSLGMGSRLPAYCTSNGRLLLAHQPA
HHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEEEEEECCH
SVLDGFFEHADLRPRTLQTKVSRQELEACFERARELDYVVVDEELEPGLRAMAVPVRSAA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCC
GIVLAGISVSVRAAKVTEAEMVSRLLPPMREAAAAIGRLITS
CEEEECCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]