Click here to switch to the map view.

The map label for this gene is nac [H]

Identifier: 73538974

GI number: 73538974

Start: 1890772

End: 1891698

Strand: Reverse

Name: nac [H]

Synonym: Reut_B5149

Alternate gene names: 73538974

Gene position: 1891698-1890772 (Counterclockwise)

Preceding gene: 73538979

Following gene: 73538973

Centisome position: 69.39

GC content: 66.34

Gene sequence:

>927_bases
ATGGAGCTTCGGCAGTTGCGGTATTTCCTGCAGGTAGTCGAACTCGGCAGCATGGGAAAGGCTGCGCAGAAGCTGGACGT
GGGCACATCGGCACTTAGTCAGCAGATCAGCAGGCTCGAAGGCGAGTTGGCGACTCGGCTCTTGCAGCGCACGTCCGCAG
GCGTGGTGCCGACGGACGCCGGGCTGGCTTTTTGGCGGCAGGCGCAACTGGCCGTCCGCCATGCCGACGATGCGGTCCGT
GCCGCGCAGCTCGCGAGACTGTCTGGCCACGTCAGCGTCGGCATGGCGCCCAGTACGATCTCGGTGCTTGGCTTGCCTTT
CATGCAGGCCATGCGGGACCGCTATCCGGACATCCGGCTTCATCTCGTCGAAAGTCTCTCGGGCAACCTGGCGACCATGA
TCGACACCCGCCAACTTGACCTCGCCGTCCTGTTCCAGGCGGACGGACACCACCGCTGGAGTCTGGCGCCACTGCTTACG
GAACGTCTTTTCCTGATCGGCCGGCGCGACATGCCGGGCGTGCCAGCGGGTAAGACCGTGAAGCTGGCGCAGTTGCACGA
TTTGCCGCTGATCCTGCCAAGCAGTCCGCACGGATTGCGGTCGGTGCTCGATGTGGCATTTGCGAGGGGAAAGTTCCAGC
CAAACCTGGTGGCCGAGATCGACGGACTCGCGATGCTGATGGATGCCGTGCGGGCAGGCTTCGGGGCGACCGTGCAGCCC
GGCGCGGCAGTCGCGCGGCTCGGCGGCGAGCCGCTTGCCATGATTGCCATCGCGGACGCCGGCGCACGCCGGCCCAATGT
GCTGGCCAGCTTGTCGGATGAAGAGCTGTCGCCGGCCGGGCTCGCCGCCCGAGGCACCCTGGCGTCCGTCGCGCGTCAGC
TGGTGCTGGACGGAAGCTGGCCAGGGGCGACCCTTCACGATTCCTAA

Upstream 100 bases:

>100_bases
AGTAATCGCTATCCTCGCAGGGGCGGGAGGGCGACCCCGCAGGGCAAGCGCGCTCGACAATCGGCCGCGCCTTACGGAAA
GTGCAAGAAGGAGAAAACGA

Downstream 100 bases:

>100_bases
ACACCTGTTTCCCGCCCCCTTCTGGCGGGCGCCCGCCCCGCTCAGTACAGTCGCAGCCAACAACGGAGACTGTACTCATG
TGGGACGTACTCGTAATCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELRQLRYFLQVVELGSMGKAAQKLDVGTSALSQQISRLEGELATRLLQRTSAGVVPTDAGLAFWRQAQLAVRHADDAVR
AAQLARLSGHVSVGMAPSTISVLGLPFMQAMRDRYPDIRLHLVESLSGNLATMIDTRQLDLAVLFQADGHHRWSLAPLLT
ERLFLIGRRDMPGVPAGKTVKLAQLHDLPLILPSSPHGLRSVLDVAFARGKFQPNLVAEIDGLAMLMDAVRAGFGATVQP
GAAVARLGGEPLAMIAIADAGARRPNVLASLSDEELSPAGLAARGTLASVARQLVLDGSWPGATLHDS

Sequences:

>Translated_308_residues
MELRQLRYFLQVVELGSMGKAAQKLDVGTSALSQQISRLEGELATRLLQRTSAGVVPTDAGLAFWRQAQLAVRHADDAVR
AAQLARLSGHVSVGMAPSTISVLGLPFMQAMRDRYPDIRLHLVESLSGNLATMIDTRQLDLAVLFQADGHHRWSLAPLLT
ERLFLIGRRDMPGVPAGKTVKLAQLHDLPLILPSSPHGLRSVLDVAFARGKFQPNLVAEIDGLAMLMDAVRAGFGATVQP
GAAVARLGGEPLAMIAIADAGARRPNVLASLSDEELSPAGLAARGTLASVARQLVLDGSWPGATLHDS
>Mature_308_residues
MELRQLRYFLQVVELGSMGKAAQKLDVGTSALSQQISRLEGELATRLLQRTSAGVVPTDAGLAFWRQAQLAVRHADDAVR
AAQLARLSGHVSVGMAPSTISVLGLPFMQAMRDRYPDIRLHLVESLSGNLATMIDTRQLDLAVLFQADGHHRWSLAPLLT
ERLFLIGRRDMPGVPAGKTVKLAQLHDLPLILPSSPHGLRSVLDVAFARGKFQPNLVAEIDGLAMLMDAVRAGFGATVQP
GAAVARLGGEPLAMIAIADAGARRPNVLASLSDEELSPAGLAARGTLASVARQLVLDGSWPGATLHDS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=36.5079365079365, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI157672245, Length=270, Percent_Identity=31.4814814814815, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787601, Length=206, Percent_Identity=31.0679611650485, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788748, Length=265, Percent_Identity=28.6792452830189, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=307, Percent_Identity=28.9902280130293, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=28.2786885245902, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693105, Length=282, Percent_Identity=23.4042553191489, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=27.1255060728745, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=25.5060728744939, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32784; Mature: 32784

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLRYFLQVVELGSMGKAAQKLDVGTSALSQQISRLEGELATRLLQRTSAGVVPTDA
CCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GLAFWRQAQLAVRHADDAVRAAQLARLSGHVSVGMAPSTISVLGLPFMQAMRDRYPDIRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHH
HLVESLSGNLATMIDTRQLDLAVLFQADGHHRWSLAPLLTERLFLIGRRDMPGVPAGKTV
HHHHHCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEE
KLAQLHDLPLILPSSPHGLRSVLDVAFARGKFQPNLVAEIDGLAMLMDAVRAGFGATVQP
EEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GAAVARLGGEPLAMIAIADAGARRPNVLASLSDEELSPAGLAARGTLASVARQLVLDGSW
CHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PGATLHDS
CCCCCCCC
>Mature Secondary Structure
MELRQLRYFLQVVELGSMGKAAQKLDVGTSALSQQISRLEGELATRLLQRTSAGVVPTDA
CCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GLAFWRQAQLAVRHADDAVRAAQLARLSGHVSVGMAPSTISVLGLPFMQAMRDRYPDIRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHH
HLVESLSGNLATMIDTRQLDLAVLFQADGHHRWSLAPLLTERLFLIGRRDMPGVPAGKTV
HHHHHCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEE
KLAQLHDLPLILPSSPHGLRSVLDVAFARGKFQPNLVAEIDGLAMLMDAVRAGFGATVQP
EEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GAAVARLGGEPLAMIAIADAGARRPNVLASLSDEELSPAGLAARGTLASVARQLVLDGSW
CHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
PGATLHDS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]