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The map label for this gene is flbD [H]

Identifier: 73538902

GI number: 73538902

Start: 1815555

End: 1816571

Strand: Direct

Name: flbD [H]

Synonym: Reut_B5077

Alternate gene names: 73538902

Gene position: 1815555-1816571 (Clockwise)

Preceding gene: 73538896

Following gene: 73538904

Centisome position: 66.6

GC content: 66.18

Gene sequence:

>1017_bases
GTGTCGACTACCGCCAGTCCGTCCCGACGCGGCGCGGCCCCGCAAGCGGAGGGCTTGCGGGGCCGCGCCATTCCCTCGCA
GGTCTCCCTGTTGTGGGAAAGCACCTCGCCCGCCATGCGCCGCCTGCTCGCGCAGGTCGAACGTGTCGCCGCTACCGATG
TCACCATGATCGCCGTCGGGGAAAGCGGCACCGGCAAGGAAGTCGTGGCGCGGGCCGTACACGCGCGCAGCAGCCGGTGC
GAAGGACCGTTCGTGGCGGTCAACTGCGGCGCAATTCAACCTACGCTGATCGAGTCCGAACTGTTCGGCCACGAAAAGGG
CGGCTTCACCGGCGCGATCGACCAGAAGGCTGGCTACTTCGAACAGGCGCAGGGCGGCACGCTGTTCCTCGACGAGGTAA
CGGAAATGCCGCTCGCGATGCAGGTCAAACTGTTGCGCGTTCTGGAAAGCTGTACCTTCCACCGCGTCGGCGGCGATACG
ATGATCGCGACCGATGTCCGCATTCTCGCCGCCACCAACCGCGACCCGCTCGACGCCGTGCGCGGCGGCCAATTGCGCGA
AGACCTGCTCTACCGCCTGGCGGTATTTCCGCTGCATATCCCGCCGTTGCGGGAGCGCGTGGAAGACATCATTCCGCTCG
CCCGGCACTTCCTGGCCGAGTACAACGCGATGGAGCGGACCGACAAGGTGTTTTCCGCCGGCACGCTTGAGCGGCTCACG
CGCTACGACTGGCCCGGCAATGTGCGCGAACTCAAGAATGCGGTCTACCGCGCGTTCATCCTCGCCGACAAGGTGATCGA
GATCGGCAATCCCAACCTCGGCTCGCAGCCGCAGCGGCCGAACACGGTAGACGGTGTCGTCAACGTGCGTGTCGGCACCA
CGCTTGCCGACACGCAGCGCGAGATCATCATGGCCACGCTCGCCCACTACAACGGCGACAAGCGGCAGGCCGCGCGGGCA
CTCGGCATCAGCCTGAAGACGTTGTACAACCGCCTTGAAGCGTACCGGGCCGGCTGA

Upstream 100 bases:

>100_bases
ACGGCTGTCGGATGTTTCCGCGCGCTGCGCCTGACGTCTGGCACCGCCTTGCCCCACGGGCTAGCATAGGAACGTCCCGC
GCCCCGTTATCCATGCCAAC

Downstream 100 bases:

>100_bases
GGGCCGGCAACGGTGGCAATGCGTGCCAGGCCTAGTCATGCCGTCCGCGGCGAACCTGCATCACCGCGGCCAGTGCGGCC
GACGCGCCGACAACGAGCGC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSTTASPSRRGAAPQAEGLRGRAIPSQVSLLWESTSPAMRRLLAQVERVAATDVTMIAVGESGTGKEVVARAVHARSSRC
EGPFVAVNCGAIQPTLIESELFGHEKGGFTGAIDQKAGYFEQAQGGTLFLDEVTEMPLAMQVKLLRVLESCTFHRVGGDT
MIATDVRILAATNRDPLDAVRGGQLREDLLYRLAVFPLHIPPLRERVEDIIPLARHFLAEYNAMERTDKVFSAGTLERLT
RYDWPGNVRELKNAVYRAFILADKVIEIGNPNLGSQPQRPNTVDGVVNVRVGTTLADTQREIIMATLAHYNGDKRQAARA
LGISLKTLYNRLEAYRAG

Sequences:

>Translated_338_residues
MSTTASPSRRGAAPQAEGLRGRAIPSQVSLLWESTSPAMRRLLAQVERVAATDVTMIAVGESGTGKEVVARAVHARSSRC
EGPFVAVNCGAIQPTLIESELFGHEKGGFTGAIDQKAGYFEQAQGGTLFLDEVTEMPLAMQVKLLRVLESCTFHRVGGDT
MIATDVRILAATNRDPLDAVRGGQLREDLLYRLAVFPLHIPPLRERVEDIIPLARHFLAEYNAMERTDKVFSAGTLERLT
RYDWPGNVRELKNAVYRAFILADKVIEIGNPNLGSQPQRPNTVDGVVNVRVGTTLADTQREIIMATLAHYNGDKRQAARA
LGISLKTLYNRLEAYRAG
>Mature_337_residues
STTASPSRRGAAPQAEGLRGRAIPSQVSLLWESTSPAMRRLLAQVERVAATDVTMIAVGESGTGKEVVARAVHARSSRCE
GPFVAVNCGAIQPTLIESELFGHEKGGFTGAIDQKAGYFEQAQGGTLFLDEVTEMPLAMQVKLLRVLESCTFHRVGGDTM
IATDVRILAATNRDPLDAVRGGQLREDLLYRLAVFPLHIPPLRERVEDIIPLARHFLAEYNAMERTDKVFSAGTLERLTR
YDWPGNVRELKNAVYRAFILADKVIEIGNPNLGSQPQRPNTVDGVVNVRVGTTLADTQREIIMATLAHYNGDKRQAARAL
GISLKTLYNRLEAYRAG

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=326, Percent_Identity=41.4110429447853, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1790437, Length=301, Percent_Identity=44.8504983388704, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI87082117, Length=227, Percent_Identity=52.863436123348, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI87082152, Length=312, Percent_Identity=42.6282051282051, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789087, Length=304, Percent_Identity=43.421052631579, Blast_Score=227, Evalue=8e-61,
Organism=Escherichia coli, GI1790299, Length=321, Percent_Identity=41.4330218068536, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI1788905, Length=241, Percent_Identity=46.8879668049792, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789233, Length=313, Percent_Identity=40.8945686900958, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=40.4388714733542, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1787583, Length=231, Percent_Identity=43.2900432900433, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=235, Percent_Identity=45.531914893617, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789828, Length=271, Percent_Identity=30.9963099630996, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=27.9605263157895, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 37008; Mature: 36877

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTASPSRRGAAPQAEGLRGRAIPSQVSLLWESTSPAMRRLLAQVERVAATDVTMIAVG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEC
ESGTGKEVVARAVHARSSRCEGPFVAVNCGAIQPTLIESELFGHEKGGFTGAIDQKAGYF
CCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
EQAQGGTLFLDEVTEMPLAMQVKLLRVLESCTFHRVGGDTMIATDVRILAATNRDPLDAV
HHCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEEECEEEEEECCCCHHHHH
RGGQLREDLLYRLAVFPLHIPPLRERVEDIIPLARHFLAEYNAMERTDKVFSAGTLERLT
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYDWPGNVRELKNAVYRAFILADKVIEIGNPNLGSQPQRPNTVDGVVNVRVGTTLADTQR
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHH
EIIMATLAHYNGDKRQAARALGISLKTLYNRLEAYRAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTASPSRRGAAPQAEGLRGRAIPSQVSLLWESTSPAMRRLLAQVERVAATDVTMIAVG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEEC
ESGTGKEVVARAVHARSSRCEGPFVAVNCGAIQPTLIESELFGHEKGGFTGAIDQKAGYF
CCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
EQAQGGTLFLDEVTEMPLAMQVKLLRVLESCTFHRVGGDTMIATDVRILAATNRDPLDAV
HHCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEEECEEEEEECCCCHHHHH
RGGQLREDLLYRLAVFPLHIPPLRERVEDIIPLARHFLAEYNAMERTDKVFSAGTLERLT
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYDWPGNVRELKNAVYRAFILADKVIEIGNPNLGSQPQRPNTVDGVVNVRVGTTLADTQR
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHH
EIIMATLAHYNGDKRQAARALGISLKTLYNRLEAYRAG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]