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The map label for this gene is pcaK [H]

Identifier: 73538852

GI number: 73538852

Start: 1759213

End: 1760586

Strand: Reverse

Name: pcaK [H]

Synonym: Reut_B5027

Alternate gene names: 73538852

Gene position: 1760586-1759213 (Counterclockwise)

Preceding gene: 73538853

Following gene: 73538850

Centisome position: 64.58

GC content: 67.32

Gene sequence:

>1374_bases
GTGCCCCAGTCCCAACCCGCCAAAGGCGGCGTCATCGACGTCCAGGCTCTGATCGACGAGCGGCCGTTCACCGGCTACCA
GTGGCTGATCCTGATCCTGTGCTTCCTGATCGTTGCGGTCGATGGTTTCGACACAGCGGCCATTGGCTATATCGCGCCGG
CGCTGGTGCAGGACTGGGGTATCCAGAAGTCGGAACTCGGCCCCGTCATGAGCGCCGCGCTGTTCGGCCTGGCCTTCGGC
GCCATCTTCGCCGGTCCGCTCGCTGACCGCGTCGGCCGCAAGCGCGTGCTGGTGCTTTCGGTGTTCTTCTTCGGCCTGTG
CAGCGTGGCCACAGCCTTCGCGCCAAGCCTTGGCTGGCTGACGGCGCTGCGCTTCCTGACCGGGCTCGGCCTTGGTGCCG
CCATGCCGAACGCGGTGACGCTGACCTCCGAATTCGCTCCGCAGCGTCGCCGCGCCGTGCTGGTCAACACCATGTTCTGC
GGCTTTCCGCTCGGTTCCGCCGTGGGCGGCTTTGTCGCGGCGGCGATGATTCCGCACTACGGCTGGCACAGTGTGCTGCT
GCTCGGCGGCGTGCTGCCGGTCGTGCTGGCCGTGCTGCTGGTGTTCCTGCTGCCGGAATCGGTGCGGTACATGGTGCTGC
GCAGCTACCCGGTCGAACAGATCGGCAAGGTGCTGCAGCGGGTTACGGGTGCGGCCACCACGGCGACCGGCTATGTCATC
GGCGAGCAGGCCCCGGCCACGCGCTCGCCCATCGGCACGGTGCTGTCGCCCGGCTATCGCCTGGGCTCGGCCATGCTGTG
GAGCACCTACTTCATGGGTCTCGTCATCTTCTACCTGCTGACGAGCTGGATGCCCACGCTGATGAAGGACGCGGGCTTCA
CGATCGAGCGTGCCTCGTTCCTGACCGCGCTGTTCCCGCTCGGCGGCGGCATCGGCACCATCGCCGCTGGCTGGTTCATG
GACCGCTTCAATCCGAACAAGGTCATTGCGTTGACGTATGCCGCGACAGGCGTGCTGATCTACGCGGTCGGGCAGGGCAC
CGGCAGCCAGGTGCTGCTCGGCGTACTGATCTTCCTGGCCGGCACGGCAATGAATGGTGCCCAGTCGTCGATGCCGTCGC
TCGCCGCGGCGTTCTATCCGACGCAGGGCCGTGCGACCGGCGTGGCGTGGATGCTCGGCATCGGCCGCTTCGGCGGCATC
ACAGGCGCGCTGCTCGGTGCGGAACTGCTGCGCCTGCAGCTGGGCTTCGACATGATCTTCACGTTGCTTGCGGTGCCGGC
GTTCCTGGCAGCGATTGCGCTGATGGTGAAACACTTCGGCGGCCGTCCGGCAGGGACCACCGAGACGGGTGGCCGGCCCG
TGGCCACTCACTGA

Upstream 100 bases:

>100_bases
AGATCGGTTCCATTAGAGAACCTCGGTTCGCTATAAGAACAAATGGCGGGCTGCCGAGACAGCGGCCGAGCCGATTTCCT
GAATCAAACGGAGACAAACC

Downstream 100 bases:

>100_bases
GACGGGAGCGGTTGTCCGCGACGCGGGGCATCGGGATGCGCCGCCGCCCGGATGCAATCGGCCAGCGCCTCGGCCATCGG
CGTGAGAGTGCGGTCCTTGT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPQSQPAKGGVIDVQALIDERPFTGYQWLILILCFLIVAVDGFDTAAIGYIAPALVQDWGIQKSELGPVMSAALFGLAFG
AIFAGPLADRVGRKRVLVLSVFFFGLCSVATAFAPSLGWLTALRFLTGLGLGAAMPNAVTLTSEFAPQRRRAVLVNTMFC
GFPLGSAVGGFVAAAMIPHYGWHSVLLLGGVLPVVLAVLLVFLLPESVRYMVLRSYPVEQIGKVLQRVTGAATTATGYVI
GEQAPATRSPIGTVLSPGYRLGSAMLWSTYFMGLVIFYLLTSWMPTLMKDAGFTIERASFLTALFPLGGGIGTIAAGWFM
DRFNPNKVIALTYAATGVLIYAVGQGTGSQVLLGVLIFLAGTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFGGI
TGALLGAELLRLQLGFDMIFTLLAVPAFLAAIALMVKHFGGRPAGTTETGGRPVATH

Sequences:

>Translated_457_residues
MPQSQPAKGGVIDVQALIDERPFTGYQWLILILCFLIVAVDGFDTAAIGYIAPALVQDWGIQKSELGPVMSAALFGLAFG
AIFAGPLADRVGRKRVLVLSVFFFGLCSVATAFAPSLGWLTALRFLTGLGLGAAMPNAVTLTSEFAPQRRRAVLVNTMFC
GFPLGSAVGGFVAAAMIPHYGWHSVLLLGGVLPVVLAVLLVFLLPESVRYMVLRSYPVEQIGKVLQRVTGAATTATGYVI
GEQAPATRSPIGTVLSPGYRLGSAMLWSTYFMGLVIFYLLTSWMPTLMKDAGFTIERASFLTALFPLGGGIGTIAAGWFM
DRFNPNKVIALTYAATGVLIYAVGQGTGSQVLLGVLIFLAGTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFGGI
TGALLGAELLRLQLGFDMIFTLLAVPAFLAAIALMVKHFGGRPAGTTETGGRPVATH
>Mature_456_residues
PQSQPAKGGVIDVQALIDERPFTGYQWLILILCFLIVAVDGFDTAAIGYIAPALVQDWGIQKSELGPVMSAALFGLAFGA
IFAGPLADRVGRKRVLVLSVFFFGLCSVATAFAPSLGWLTALRFLTGLGLGAAMPNAVTLTSEFAPQRRRAVLVNTMFCG
FPLGSAVGGFVAAAMIPHYGWHSVLLLGGVLPVVLAVLLVFLLPESVRYMVLRSYPVEQIGKVLQRVTGAATTATGYVIG
EQAPATRSPIGTVLSPGYRLGSAMLWSTYFMGLVIFYLLTSWMPTLMKDAGFTIERASFLTALFPLGGGIGTIAAGWFMD
RFNPNKVIALTYAATGVLIYAVGQGTGSQVLLGVLIFLAGTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFGGIT
GALLGAELLRLQLGFDMIFTLLAVPAFLAAIALMVKHFGGRPAGTTETGGRPVATH

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=414, Percent_Identity=25.3623188405797, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI7662270, Length=235, Percent_Identity=25.1063829787234, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI216548223, Length=370, Percent_Identity=24.8648648648649, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI73695465, Length=214, Percent_Identity=28.9719626168224, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI213021148, Length=173, Percent_Identity=27.1676300578035, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI166064021, Length=235, Percent_Identity=25.9574468085106, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI8923870, Length=363, Percent_Identity=24.5179063360882, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI87081723, Length=388, Percent_Identity=35.5670103092784, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87082231, Length=426, Percent_Identity=24.8826291079812, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082159, Length=390, Percent_Identity=26.1538461538462, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788074, Length=363, Percent_Identity=24.5179063360882, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788068, Length=457, Percent_Identity=23.6323851203501, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=175, Percent_Identity=29.1428571428571, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786229, Length=209, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789207, Length=192, Percent_Identity=32.2916666666667, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789152, Length=326, Percent_Identity=22.6993865030675, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082320, Length=300, Percent_Identity=20.6666666666667, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=432, Percent_Identity=25, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922874, Length=371, Percent_Identity=25.6064690026954, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640198, Length=224, Percent_Identity=26.3392857142857, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640196, Length=224, Percent_Identity=26.3392857142857, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640200, Length=224, Percent_Identity=26.3392857142857, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48029; Mature: 47898

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSQPAKGGVIDVQALIDERPFTGYQWLILILCFLIVAVDGFDTAAIGYIAPALVQDWG
CCCCCCCCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
IQKSELGPVMSAALFGLAFGAIFAGPLADRVGRKRVLVLSVFFFGLCSVATAFAPSLGWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
TALRFLTGLGLGAAMPNAVTLTSEFAPQRRRAVLVNTMFCGFPLGSAVGGFVAAAMIPHY
HHHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
GWHSVLLLGGVLPVVLAVLLVFLLPESVRYMVLRSYPVEQIGKVLQRVTGAATTATGYVI
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCEEE
GEQAPATRSPIGTVLSPGYRLGSAMLWSTYFMGLVIFYLLTSWMPTLMKDAGFTIERASF
ECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LTALFPLGGGIGTIAAGWFMDRFNPNKVIALTYAATGVLIYAVGQGTGSQVLLGVLIFLA
HHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
GTAMNGAQSSMPSLAAAFYPTQGRATGVAWMLGIGRFGGITGALLGAELLRLQLGFDMIF
HCCCCCCHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TLLAVPAFLAAIALMVKHFGGRPAGTTETGGRPVATH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PQSQPAKGGVIDVQALIDERPFTGYQWLILILCFLIVAVDGFDTAAIGYIAPALVQDWG
CCCCCCCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC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HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]