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The map label for this gene is pcaG [H]

Identifier: 73538849

GI number: 73538849

Start: 1756871

End: 1757440

Strand: Reverse

Name: pcaG [H]

Synonym: Reut_B5024

Alternate gene names: 73538849

Gene position: 1757440-1756871 (Counterclockwise)

Preceding gene: 73538850

Following gene: 73538848

Centisome position: 64.47

GC content: 69.47

Gene sequence:

>570_bases
ATGCTGTACGCCACCGCTTCGCAAACTGTCGGCCCTTACCTGCATATCGGCCTGACCGGCCTGAACTGCGCCGACCTCAC
CGCTGACGCACCGGAACTGGCCGGCCAGCGCATCGTCATCGAAGGCCGCGTCGTCGACGGCACCGGCGCGCCCGTGCCCG
ACGGCATGGTCGAGATCTGGCAAGCCAACCCGGCCGGCCGCTACCGCCATGCCGAAGATGCGCGCGAACTGCCGCTGGTT
GCCGGCTTCACCGGCTTCGGCCGCGTGCCGACCGCCGAGGACGGCTCGTTCCGCTTCGTCACCGTGAAGCCGGGCGTGGT
GCCGGGCGCGGACGGCAAGCCGCAGGCGCCGCACATCATGGTGTCGGTGTTTATGCGCGGCCTGGTCAAGCACGTGGCCA
CGCGCGTCTACTTCCCGGACGAGACCGCTGCCAATGCCGCCGACGGCGTGCTGGCACTGGTGCCGGCGGAGCGCCGCGGC
ACGCTGATCGCTACCGCGCAAGGTAATGGCACGCTGCGTTGGGACGTGGTGCTGCAGGGGGCGGGTGAGACGGTGTTCTT
CGATATCTGA

Upstream 100 bases:

>100_bases
TCGACCTTTGACTGGGAAACCACGCAGCCCGAGTACGCGCTCGGTTACCGTTTCGATATCGTCCTGCGTGGCCGCGATGC
CACGCCGATGGAGTAAGAGC

Downstream 100 bases:

>100_bases
CGCTGCGCGCATAATCGTGCCGTCGCCAATGTCGTGACGGCACACCCACTACGCCCCCCAATGCCAAGCTCGACCCGCCT
TACCGATCCGATGTTCGGCA

Product: protocatechuate 3,4-dioxygenase subunit alpha

Products: NA

Alternate protein names: 3,4-PCD [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MLYATASQTVGPYLHIGLTGLNCADLTADAPELAGQRIVIEGRVVDGTGAPVPDGMVEIWQANPAGRYRHAEDARELPLV
AGFTGFGRVPTAEDGSFRFVTVKPGVVPGADGKPQAPHIMVSVFMRGLVKHVATRVYFPDETAANAADGVLALVPAERRG
TLIATAQGNGTLRWDVVLQGAGETVFFDI

Sequences:

>Translated_189_residues
MLYATASQTVGPYLHIGLTGLNCADLTADAPELAGQRIVIEGRVVDGTGAPVPDGMVEIWQANPAGRYRHAEDARELPLV
AGFTGFGRVPTAEDGSFRFVTVKPGVVPGADGKPQAPHIMVSVFMRGLVKHVATRVYFPDETAANAADGVLALVPAERRG
TLIATAQGNGTLRWDVVLQGAGETVFFDI
>Mature_189_residues
MLYATASQTVGPYLHIGLTGLNCADLTADAPELAGQRIVIEGRVVDGTGAPVPDGMVEIWQANPAGRYRHAEDARELPLV
AGFTGFGRVPTAEDGSFRFVTVKPGVVPGADGKPQAPHIMVSVFMRGLVKHVATRVYFPDETAANAADGVLALVPAERRG
TLIATAQGNGTLRWDVVLQGAGETVFFDI

Specific function: Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889
- InterPro:   IPR012786 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: =1.13.11.3 [H]

Molecular weight: Translated: 19895; Mature: 19895

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00083 INTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYATASQTVGPYLHIGLTGLNCADLTADAPELAGQRIVIEGRVVDGTGAPVPDGMVEIW
CEEECCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCEEEE
QANPAGRYRHAEDARELPLVAGFTGFGRVPTAEDGSFRFVTVKPGVVPGADGKPQAPHIM
ECCCCCCCCCCCCHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
VSVFMRGLVKHVATRVYFPDETAANAADGVLALVPAERRGTLIATAQGNGTLRWDVVLQG
HHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEEEEC
AGETVFFDI
CCCEEEEEC
>Mature Secondary Structure
MLYATASQTVGPYLHIGLTGLNCADLTADAPELAGQRIVIEGRVVDGTGAPVPDGMVEIW
CEEECCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCEEEE
QANPAGRYRHAEDARELPLVAGFTGFGRVPTAEDGSFRFVTVKPGVVPGADGKPQAPHIM
ECCCCCCCCCCCCHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
VSVFMRGLVKHVATRVYFPDETAANAADGVLALVPAERRGTLIATAQGNGTLRWDVVLQG
HHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEEEEEC
AGETVFFDI
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407791; 465136; 3194022; 7990141; 9254599; 9254600; 9298971 [H]