Click here to switch to the map view.

The map label for this gene is pcaB [H]

Identifier: 73538848

GI number: 73538848

Start: 1755422

End: 1756810

Strand: Reverse

Name: pcaB [H]

Synonym: Reut_B5023

Alternate gene names: 73538848

Gene position: 1756810-1755422 (Counterclockwise)

Preceding gene: 73538849

Following gene: 73538847

Centisome position: 64.44

GC content: 69.4

Gene sequence:

>1389_bases
ATGCCAAGCTCGACCCGCCTTACCGATCCGATGTTCGGCAGCCCGGCGGTGCTGGAGATCTTCTCCAATACCGGCACCGT
GCGCTGCATGCTTGCCGTCGAAGCGGCACTGGCCCGCGCCGAATCACATTGCGGCGTGATTCCCGTCGCCGCGGCCACTG
TCATCTCCGAAGTCTGTTCGGACGTCTCAGTCATCGATCTCGATGCCTTGGCCCAGGCCGCCGTTTCAGCGGGCAATCTC
GCCATTCCCTTCGTCAAGCAGCTCACTGCCGCCGTGGCGGCGCGCGATGCCGAGGCTGCGCGCTTCGTGCACTGGGGCGC
CACCAGCCAGGACATCATCGACACCGCACTGGTGCTGCAGCTTGGCGAGGCCCTGCCGCAGCTTCAGGCGGCATTGCTCG
CGCTTGGCAATGCCTGCGCCGGCCAGGCCACGTCGCATCGCGACACGCCGATGGTCGGCCGCACGTGGCTGCAGCACGCG
TTGCCAACGACGTTCGGCCTGAAGGCTGCGGGCTGGCTCGATGCACTGCGCCGAGACCTGCGCCGTCTCGATGCTGCACG
CGATCATAGCCAAGCGCTGCAGTTCGGCGGCGCAGCCGGCACACTCGCGAGCCTCGGCAAGTCCGCGCCGGACGTGGCGC
GTGCGTTGGTCAGTGAACTGAACCTGGAGTTGCCCGCGCTGCCGTGGCACGCGCATCGCGACCGACTCGTGGAAACTGCG
ACCGCGCTGGGCATGCTCACCGGCACGCTCGGCAAGATGGCGCGCGATATCTCGCTGATGATGCAGACCGAAGTCGCGGA
AGTGGCCGAGCCATCCGGCCCCGGCCGCGGTGGCTCAAGCACCATGCCGCACAAGCGCAACCCGGTCGGCTGCGCCGCCG
TGCTGACCGCGGCGGTACGTGTGCCGCCGCTGGTCGCGACGATGCTGTCGGGCATGGTGCAGGAACACGAACGCGCGCTC
GGCGGCTGGCAGGCCGAGTGGGACACGCTGCCGCAGGTCGTCGCGCTTGCCGCCGGCGCCTTGCGCCAGATGGGCGAGGT
GGTCTCGGGCCTGCAGGTCGACGCCGCGCGCATGCGCGCCAATCTCGACATGACGCATGGCCTGATCCTCGGCGAGGCGG
CCATGCTCGAACTCGGCGGCCGCATCGGCCGGCTCGAAGCGCACCATGTGGTCGAGCGCGCATCGCGCCGCGCGGTGGCG
CAAGGCACCACGTTGCGCGATGCGCTGGCACAGACGCTTGCCGAGAATCCGTCGCACGCCAGTCTGCTTGACGATGCCGC
GCTCGACAGACTGGCGGATCCGGCCAACTACGCCGGCCAGTCCGCTGCATTCGCCGATGCCGCCGTGCAGGCCTGGCATG
CGTCGCTGGCGCAGCGGCAGCAGCGCTGA

Upstream 100 bases:

>100_bases
GCTGCAGGGGGCGGGTGAGACGGTGTTCTTCGATATCTGACGCTGCGCGCATAATCGTGCCGTCGCCAATGTCGTGACGG
CACACCCACTACGCCCCCCA

Downstream 100 bases:

>100_bases
ACACAACACGCCCCCCAAGGTTTCCAGGAGACCGCATTGCCTTACCTCGACCATGCCGGCGCCCGGCTTTACTACACCGT
GGACGGCCCCGACAACGCGC

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MPSSTRLTDPMFGSPAVLEIFSNTGTVRCMLAVEAALARAESHCGVIPVAAATVISEVCSDVSVIDLDALAQAAVSAGNL
AIPFVKQLTAAVAARDAEAARFVHWGATSQDIIDTALVLQLGEALPQLQAALLALGNACAGQATSHRDTPMVGRTWLQHA
LPTTFGLKAAGWLDALRRDLRRLDAARDHSQALQFGGAAGTLASLGKSAPDVARALVSELNLELPALPWHAHRDRLVETA
TALGMLTGTLGKMARDISLMMQTEVAEVAEPSGPGRGGSSTMPHKRNPVGCAAVLTAAVRVPPLVATMLSGMVQEHERAL
GGWQAEWDTLPQVVALAAGALRQMGEVVSGLQVDAARMRANLDMTHGLILGEAAMLELGGRIGRLEAHHVVERASRRAVA
QGTTLRDALAQTLAENPSHASLLDDAALDRLADPANYAGQSAAFADAAVQAWHASLAQRQQR

Sequences:

>Translated_462_residues
MPSSTRLTDPMFGSPAVLEIFSNTGTVRCMLAVEAALARAESHCGVIPVAAATVISEVCSDVSVIDLDALAQAAVSAGNL
AIPFVKQLTAAVAARDAEAARFVHWGATSQDIIDTALVLQLGEALPQLQAALLALGNACAGQATSHRDTPMVGRTWLQHA
LPTTFGLKAAGWLDALRRDLRRLDAARDHSQALQFGGAAGTLASLGKSAPDVARALVSELNLELPALPWHAHRDRLVETA
TALGMLTGTLGKMARDISLMMQTEVAEVAEPSGPGRGGSSTMPHKRNPVGCAAVLTAAVRVPPLVATMLSGMVQEHERAL
GGWQAEWDTLPQVVALAAGALRQMGEVVSGLQVDAARMRANLDMTHGLILGEAAMLELGGRIGRLEAHHVVERASRRAVA
QGTTLRDALAQTLAENPSHASLLDDAALDRLADPANYAGQSAAFADAAVQAWHASLAQRQQR
>Mature_461_residues
PSSTRLTDPMFGSPAVLEIFSNTGTVRCMLAVEAALARAESHCGVIPVAAATVISEVCSDVSVIDLDALAQAAVSAGNLA
IPFVKQLTAAVAARDAEAARFVHWGATSQDIIDTALVLQLGEALPQLQAALLALGNACAGQATSHRDTPMVGRTWLQHAL
PTTFGLKAAGWLDALRRDLRRLDAARDHSQALQFGGAAGTLASLGKSAPDVARALVSELNLELPALPWHAHRDRLVETAT
ALGMLTGTLGKMARDISLMMQTEVAEVAEPSGPGRGGSSTMPHKRNPVGCAAVLTAAVRVPPLVATMLSGMVQEHERALG
GWQAEWDTLPQVVALAAGALRQMGEVVSGLQVDAARMRANLDMTHGLILGEAAMLELGGRIGRLEAHHVVERASRRAVAQ
GTTLRDALAQTLAENPSHASLLDDAALDRLADPANYAGQSAAFADAAVQAWHASLAQRQQR

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Escherichia coli, GI1787896, Length=210, Percent_Identity=29.0476190476191, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=370, Percent_Identity=27.8378378378378, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647570, Length=298, Percent_Identity=28.8590604026846, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI78710009, Length=321, Percent_Identity=26.791277258567, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640179, Length=332, Percent_Identity=26.2048192771084, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24640177, Length=332, Percent_Identity=26.2048192771084, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48301; Mature: 48170

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSTRLTDPMFGSPAVLEIFSNTGTVRCMLAVEAALARAESHCGVIPVAAATVISEVCS
CCCCCCCCCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
DVSVIDLDALAQAAVSAGNLAIPFVKQLTAAVAARDAEAARFVHWGATSQDIIDTALVLQ
CCCEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHEECCCCHHHHHHHHHHHH
LGEALPQLQAALLALGNACAGQATSHRDTPMVGRTWLQHALPTTFGLKAAGWLDALRRDL
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHH
RRLDAARDHSQALQFGGAAGTLASLGKSAPDVARALVSELNLELPALPWHAHRDRLVETA
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TALGMLTGTLGKMARDISLMMQTEVAEVAEPSGPGRGGSSTMPHKRNPVGCAAVLTAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VPPLVATMLSGMVQEHERALGGWQAEWDTLPQVVALAAGALRQMGEVVSGLQVDAARMRA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
NLDMTHGLILGEAAMLELGGRIGRLEAHHVVERASRRAVAQGTTLRDALAQTLAENPSHA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
SLLDDAALDRLADPANYAGQSAAFADAAVQAWHASLAQRQQR
HHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSSTRLTDPMFGSPAVLEIFSNTGTVRCMLAVEAALARAESHCGVIPVAAATVISEVCS
CCCCCCCCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
DVSVIDLDALAQAAVSAGNLAIPFVKQLTAAVAARDAEAARFVHWGATSQDIIDTALVLQ
CCCEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHEECCCCHHHHHHHHHHHH
LGEALPQLQAALLALGNACAGQATSHRDTPMVGRTWLQHALPTTFGLKAAGWLDALRRDL
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHH
RRLDAARDHSQALQFGGAAGTLASLGKSAPDVARALVSELNLELPALPWHAHRDRLVETA
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TALGMLTGTLGKMARDISLMMQTEVAEVAEPSGPGRGGSSTMPHKRNPVGCAAVLTAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VPPLVATMLSGMVQEHERALGGWQAEWDTLPQVVALAAGALRQMGEVVSGLQVDAARMRA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
NLDMTHGLILGEAAMLELGGRIGRLEAHHVVERASRRAVAQGTTLRDALAQTLAENPSHA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
SLLDDAALDRLADPANYAGQSAAFADAAVQAWHASLAQRQQR
HHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]