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The map label for this gene is thcD [H]

Identifier: 73538547

GI number: 73538547

Start: 1422382

End: 1423641

Strand: Direct

Name: thcD [H]

Synonym: Reut_B4722

Alternate gene names: 73538547

Gene position: 1422382-1423641 (Clockwise)

Preceding gene: 73538546

Following gene: 73538549

Centisome position: 52.18

GC content: 65.95

Gene sequence:

>1260_bases
ATGCATGACGAACAACCGCGCGGCGCGACCGCTAACGTGCCGACCAGCACGATCGTCATCGTTGGCGCAGGCCAGGCCGG
CGGCTGGGCCGCCCAGACGCTGCGCAACGAAGGCTTCACAGGCAGGCTTGTGCTGATTGGTGATGAAAGCCATCCTCCGT
ATGAGCGGCCGCCATTGTCCAAAGCCGTGCTTGCAGGAGAGGCGGAGCCTTCCAGCACGCAGTTGCTCAGGCCAGACGCG
TTCGAGGCCCTCGGGCTGGAGTGGTGGCCGGACATACCGGTGAACCACATCGACCGCCATGCGAAACGGCTGGAGATGAC
GGATGGCAAGACGCTGGCCTACGACAAGCTGATCCTGTGCACCGGCGGCCGCGCCCGCACGCTCACTGTCCCGGGCGCGG
ATCGGGCCCGCGTCCATACCTTGCGGACCATTGGTGACGCGCTGTCGCTCGCACAAGAGTTTCGACCGGGCCGCAGCGTG
GCCGTGATCGGCGGTGGCTGGATCGGACTCGAAGTCGCCGCCACGGCGCGCCAGAGAGGCGCAGAGGTGACGGTAATCGA
GGCACAAGGCCGGCTGTGCGAACGCTCCGTGCCGCCGGAGATCTCGGAGCATCTGCTTGGGCTGCATGCCACGCATGGCG
TGCACATCAGACTCGGCGTTCATATCTCTGGTATCGCCCGAGGCGCCGACGGACGGCCCGCCCTGACGCTTGCCGATGGG
GACACGCTAACTTGCGATGCGATCGTCGCGGGGGTGGGTCTGGTGCCGAATGATGATCTGGCGCGTGACGCAGGGCTGGA
TTGCGACGGCGGCATAATTGTCGACGAGCGCTGCTGCACGTCCGACCCCGACATCTTTGCCGCGGGCGATGTCGCCGTGA
CGCCAAACCCGTGGGCTGGCCGCCGCATGCGGCTGGAATCGTGGCAGAACGCGCAGGAGCAAGGTATCGCTGCGGCACGC
GCCGCGCTGTGCCTGCCGGTCGTCTATCAGCCGCTGCCGTGGTTCTGGTCTGATCAATACGATATGAACCTGCAGATCTA
TGGCGTCCCCCTGCCCTCGCACCGCATCGTGACGCGCGGCATGCCGGGAGACAACAGCTTCGCGCTGTTCTACCTCGAAG
ACGATGTCATCAAGGCCGCGCTGGGCGCCAACGCCGCCCGCGACCTCCGTTTCGCGCGCAGGCTGATCGAGCAGAGCAAG
CCGGTAGATGTGAAGCGACTTGCCGACGCGGATGTTCCCATGGGCAAGCTGTCTGCCTGA

Upstream 100 bases:

>100_bases
CTGACCGAGGGCATCCGCATCTATCCCGTGCGGATCGAGGGCGGCAAGGTCTTCGTGGACCTCGGTTAACACGCCTGCGT
CAGAACCAGAGGAACTCCGC

Downstream 100 bases:

>100_bases
AGCCGACGACGCTTCACCACCGAGCCTGATGGCAAGAGTGGTGAGCACGCGCGGCGAGCGGGTGTTCGGCAAGCGTTGCA
GGCCTGGCCGGCGCAACACG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MHDEQPRGATANVPTSTIVIVGAGQAGGWAAQTLRNEGFTGRLVLIGDESHPPYERPPLSKAVLAGEAEPSSTQLLRPDA
FEALGLEWWPDIPVNHIDRHAKRLEMTDGKTLAYDKLILCTGGRARTLTVPGADRARVHTLRTIGDALSLAQEFRPGRSV
AVIGGGWIGLEVAATARQRGAEVTVIEAQGRLCERSVPPEISEHLLGLHATHGVHIRLGVHISGIARGADGRPALTLADG
DTLTCDAIVAGVGLVPNDDLARDAGLDCDGGIIVDERCCTSDPDIFAAGDVAVTPNPWAGRRMRLESWQNAQEQGIAAAR
AALCLPVVYQPLPWFWSDQYDMNLQIYGVPLPSHRIVTRGMPGDNSFALFYLEDDVIKAALGANAARDLRFARRLIEQSK
PVDVKRLADADVPMGKLSA

Sequences:

>Translated_419_residues
MHDEQPRGATANVPTSTIVIVGAGQAGGWAAQTLRNEGFTGRLVLIGDESHPPYERPPLSKAVLAGEAEPSSTQLLRPDA
FEALGLEWWPDIPVNHIDRHAKRLEMTDGKTLAYDKLILCTGGRARTLTVPGADRARVHTLRTIGDALSLAQEFRPGRSV
AVIGGGWIGLEVAATARQRGAEVTVIEAQGRLCERSVPPEISEHLLGLHATHGVHIRLGVHISGIARGADGRPALTLADG
DTLTCDAIVAGVGLVPNDDLARDAGLDCDGGIIVDERCCTSDPDIFAAGDVAVTPNPWAGRRMRLESWQNAQEQGIAAAR
AALCLPVVYQPLPWFWSDQYDMNLQIYGVPLPSHRIVTRGMPGDNSFALFYLEDDVIKAALGANAARDLRFARRLIEQSK
PVDVKRLADADVPMGKLSA
>Mature_419_residues
MHDEQPRGATANVPTSTIVIVGAGQAGGWAAQTLRNEGFTGRLVLIGDESHPPYERPPLSKAVLAGEAEPSSTQLLRPDA
FEALGLEWWPDIPVNHIDRHAKRLEMTDGKTLAYDKLILCTGGRARTLTVPGADRARVHTLRTIGDALSLAQEFRPGRSV
AVIGGGWIGLEVAATARQRGAEVTVIEAQGRLCERSVPPEISEHLLGLHATHGVHIRLGVHISGIARGADGRPALTLADG
DTLTCDAIVAGVGLVPNDDLARDAGLDCDGGIIVDERCCTSDPDIFAAGDVAVTPNPWAGRRMRLESWQNAQEQGIAAAR
AALCLPVVYQPLPWFWSDQYDMNLQIYGVPLPSHRIVTRGMPGDNSFALFYLEDDVIKAALGANAARDLRFARRLIEQSK
PVDVKRLADADVPMGKLSA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=374, Percent_Identity=31.0160427807487, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=31.0160427807487, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=31.0160427807487, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4757732, Length=332, Percent_Identity=28.3132530120482, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI22202629, Length=332, Percent_Identity=28.3132530120482, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788892, Length=405, Percent_Identity=40.7407407407407, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789065, Length=275, Percent_Identity=33.0909090909091, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789765, Length=284, Percent_Identity=30.6338028169014, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789915, Length=223, Percent_Identity=29.5964125560538, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=407, Percent_Identity=27.7641277641278, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32564386, Length=332, Percent_Identity=26.2048192771084, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639257, Length=392, Percent_Identity=27.2959183673469, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281359715, Length=391, Percent_Identity=27.3657289002558, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281359713, Length=391, Percent_Identity=27.3657289002558, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639250, Length=391, Percent_Identity=27.3657289002558, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI18543267, Length=391, Percent_Identity=27.3657289002558, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639252, Length=391, Percent_Identity=27.3657289002558, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24585130, Length=403, Percent_Identity=25.8064516129032, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=25.2100840336134, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=25.2100840336134, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44938; Mature: 44938

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDEQPRGATANVPTSTIVIVGAGQAGGWAAQTLRNEGFTGRLVLIGDESHPPYERPPLS
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCC
KAVLAGEAEPSSTQLLRPDAFEALGLEWWPDIPVNHIDRHAKRLEMTDGKTLAYDKLILC
HHHEECCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCEEEECEEEEE
TGGRARTLTVPGADRARVHTLRTIGDALSLAQEFRPGRSVAVIGGGWIGLEVAATARQRG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEHHHHCC
AEVTVIEAQGRLCERSVPPEISEHLLGLHATHGVHIRLGVHISGIARGADGRPALTLADG
CEEEEEECCCCHHCCCCCHHHHHHHHEEEECCCEEEEEEEEEEEEEECCCCCCEEEEECC
DTLTCDAIVAGVGLVPNDDLARDAGLDCDGGIIVDERCCTSDPDIFAAGDVAVTPNPWAG
CEEEHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCCCCCCEEEECCEEECCCCCCC
RRMRLESWQNAQEQGIAAARAALCLPVVYQPLPWFWSDQYDMNLQIYGVPLPSHRIVTRG
CEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEEEEC
MPGDNSFALFYLEDDVIKAALGANAARDLRFARRLIEQSKPVDVKRLADADVPMGKLSA
CCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHDEQPRGATANVPTSTIVIVGAGQAGGWAAQTLRNEGFTGRLVLIGDESHPPYERPPLS
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCC
KAVLAGEAEPSSTQLLRPDAFEALGLEWWPDIPVNHIDRHAKRLEMTDGKTLAYDKLILC
HHHEECCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCEEEECEEEEE
TGGRARTLTVPGADRARVHTLRTIGDALSLAQEFRPGRSVAVIGGGWIGLEVAATARQRG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEHHHHCC
AEVTVIEAQGRLCERSVPPEISEHLLGLHATHGVHIRLGVHISGIARGADGRPALTLADG
CEEEEEECCCCHHCCCCCHHHHHHHHEEEECCCEEEEEEEEEEEEEECCCCCCEEEEECC
DTLTCDAIVAGVGLVPNDDLARDAGLDCDGGIIVDERCCTSDPDIFAAGDVAVTPNPWAG
CEEEHHHHHHCCCCCCCCCHHHHCCCCCCCCEEECCCCCCCCCCEEEECCEEECCCCCCC
RRMRLESWQNAQEQGIAAARAALCLPVVYQPLPWFWSDQYDMNLQIYGVPLPSHRIVTRG
CEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEEEEC
MPGDNSFALFYLEDDVIKAALGANAARDLRFARRLIEQSKPVDVKRLADADVPMGKLSA
CCCCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]