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The map label for this gene is bphA [H]

Identifier: 73538544

GI number: 73538544

Start: 1420295

End: 1421548

Strand: Direct

Name: bphA [H]

Synonym: Reut_B4719

Alternate gene names: 73538544

Gene position: 1420295-1421548 (Clockwise)

Preceding gene: 73538542

Following gene: 73538545

Centisome position: 52.1

GC content: 62.28

Gene sequence:

>1254_bases
ATGCGAACCGACTCGTCCCAGGCTGGTCCGGTGTGGGCTGACGACAGCATCAGCCGCATCCCCTTCCGCGTCTACACGGA
CGCCCAGTTGTACCGGCGTGAACTCGAACGCTGCTTCTATAAGGGTCACTGGAACTACGTCGGGCTCGAGGCCGAGATTC
CGAATGCCGGCGACTTCAAGCGCACCACGGTAGGGGAACGGTCCGTCATCCTGGTGCGCGACCAGGACGGCGACATCAAC
GTCGTCGAAAACGTCTGCGCTCATCGCGGCATGAAGTTTTGCAGGGAACGCAGCGGAAACCGCAAGGACTTCCATTGCCC
GTACCACCAGTGGAACTATGACCTGAAGGGCAACCTGCAAGGTGTGCCACTGCGCCGGGGCGTCAAGCAGGACGGCAAGG
TCAATGGCGGCATGCCGGCCGACTTCAAGCCGCAGGAGCACGGGTTGACGCAACTGAAGGTGGCGACGCGCGGCGGTGTC
GTGTTCGCTTCGTTCGACCACGACGTCCAGCCGCTCGAGGACTATCTCGGGCCGGACATCCTCGGCTACTTCGACCGACT
CTTCAATGGCCGCAAGCTGAAGGTGCTCGGCTACAACAAGCAGCGTATTCCGGGCAACTGGAAGCTGATGCAGGAAAACA
TCAAGGATCCCTACCACCCCGGCCTTCTCCACACCTGGTTTGTCACGTTCGGACTCTGGCGCGCGGACAACAAGTCGCGC
CTGGTGATGGATAACGCGTTCCGCCATGCGGCGATGGTATCGACGCGCGGCCAGGGCGGAAAAAGCGACGTGACCTCGGG
CGTCTCCAGCTTCAAGGACCAGATGGCGCTGAACGATGACCGCATCCTCGACATCGTCCACGAGCCATGGTGGGGCGAGC
CCACCGCGGTGATGATGACGGTCTTCCCCAGCGTGATCTTCCAGCAGCAGGTCAACTCGGTTTCCACGCGCCATATCCAG
CCCAATGGCCCCGACTCGTTCGACTTCGTGTGGACGCACTTCGGCTTTGACGACGACACGGACGAGATGACGCGGCGCCG
CCTGCGCCAGGCCAACCTGTTCGGGCCGGCGGGCTTCGTGTCAGCCGACGATGGCGAAGCGATCGAATGCTCGCAGGAAG
GCTTCTCCCAAAAGCCATGGCATCGCGTGATCGCCGAACTTGGCGGCAAGACCGCCGAGAACACCGACCACATGGTCACC
GAAACCCTGATCCGCGGCATGTACGCCTACTGGCGCAAGGTGATGGAGGTCTGA

Upstream 100 bases:

>100_bases
TATTGATTCTGTGAATATTGACTATAGACCGGATTCTATTGGTTTATTTTGCACGGCGCTCCATGATCCTTCGCATACGG
AAAGCAATACGGAGACAACC

Downstream 100 bases:

>100_bases
CGCCATGAACTTCACTGACTATTACGCGCTGCTCACGCTGCACGCCGATTACACCAGTGTCGTGGACAGCGGCGAATGGG
ACCGCTGGCCTGACTTCTTC

Product: ring hydroxylating dioxygenase subunit alpha

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRTDSSQAGPVWADDSISRIPFRVYTDAQLYRRELERCFYKGHWNYVGLEAEIPNAGDFKRTTVGERSVILVRDQDGDIN
VVENVCAHRGMKFCRERSGNRKDFHCPYHQWNYDLKGNLQGVPLRRGVKQDGKVNGGMPADFKPQEHGLTQLKVATRGGV
VFASFDHDVQPLEDYLGPDILGYFDRLFNGRKLKVLGYNKQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKSR
LVMDNAFRHAAMVSTRGQGGKSDVTSGVSSFKDQMALNDDRILDIVHEPWWGEPTAVMMTVFPSVIFQQQVNSVSTRHIQ
PNGPDSFDFVWTHFGFDDDTDEMTRRRLRQANLFGPAGFVSADDGEAIECSQEGFSQKPWHRVIAELGGKTAENTDHMVT
ETLIRGMYAYWRKVMEV

Sequences:

>Translated_417_residues
MRTDSSQAGPVWADDSISRIPFRVYTDAQLYRRELERCFYKGHWNYVGLEAEIPNAGDFKRTTVGERSVILVRDQDGDIN
VVENVCAHRGMKFCRERSGNRKDFHCPYHQWNYDLKGNLQGVPLRRGVKQDGKVNGGMPADFKPQEHGLTQLKVATRGGV
VFASFDHDVQPLEDYLGPDILGYFDRLFNGRKLKVLGYNKQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKSR
LVMDNAFRHAAMVSTRGQGGKSDVTSGVSSFKDQMALNDDRILDIVHEPWWGEPTAVMMTVFPSVIFQQQVNSVSTRHIQ
PNGPDSFDFVWTHFGFDDDTDEMTRRRLRQANLFGPAGFVSADDGEAIECSQEGFSQKPWHRVIAELGGKTAENTDHMVT
ETLIRGMYAYWRKVMEV
>Mature_417_residues
MRTDSSQAGPVWADDSISRIPFRVYTDAQLYRRELERCFYKGHWNYVGLEAEIPNAGDFKRTTVGERSVILVRDQDGDIN
VVENVCAHRGMKFCRERSGNRKDFHCPYHQWNYDLKGNLQGVPLRRGVKQDGKVNGGMPADFKPQEHGLTQLKVATRGGV
VFASFDHDVQPLEDYLGPDILGYFDRLFNGRKLKVLGYNKQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKSR
LVMDNAFRHAAMVSTRGQGGKSDVTSGVSSFKDQMALNDDRILDIVHEPWWGEPTAVMMTVFPSVIFQQQVNSVSTRHIQ
PNGPDSFDFVWTHFGFDDDTDEMTRRRLRQANLFGPAGFVSADDGEAIECSQEGFSQKPWHRVIAELGGKTAENTDHMVT
ETLIRGMYAYWRKVMEV

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=443, Percent_Identity=27.5395033860045, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788103, Length=209, Percent_Identity=30.1435406698565, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 47689; Mature: 47689

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDSSQAGPVWADDSISRIPFRVYTDAQLYRRELERCFYKGHWNYVGLEAEIPNAGDFK
CCCCCCCCCCEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
RTTVGERSVILVRDQDGDINVVENVCAHRGMKFCRERSGNRKDFHCPYHQWNYDLKGNLQ
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCC
GVPLRRGVKQDGKVNGGMPADFKPQEHGLTQLKVATRGGVVFASFDHDVQPLEDYLGPDI
CCCHHCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCEEEEECCCCCHHHHHHCCCHH
LGYFDRLFNGRKLKVLGYNKQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKSR
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCE
LVMDNAFRHAAMVSTRGQGGKSDVTSGVSSFKDQMALNDDRILDIVHEPWWGEPTAVMMT
EEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
VFPSVIFQQQVNSVSTRHIQPNGPDSFDFVWTHFGFDDDTDEMTRRRLRQANLFGPAGFV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
SADDGEAIECSQEGFSQKPWHRVIAELGGKTAENTDHMVTETLIRGMYAYWRKVMEV
CCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTDSSQAGPVWADDSISRIPFRVYTDAQLYRRELERCFYKGHWNYVGLEAEIPNAGDFK
CCCCCCCCCCEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
RTTVGERSVILVRDQDGDINVVENVCAHRGMKFCRERSGNRKDFHCPYHQWNYDLKGNLQ
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCC
GVPLRRGVKQDGKVNGGMPADFKPQEHGLTQLKVATRGGVVFASFDHDVQPLEDYLGPDI
CCCHHCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEECCCEEEEECCCCCHHHHHHCCCHH
LGYFDRLFNGRKLKVLGYNKQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKSR
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCCE
LVMDNAFRHAAMVSTRGQGGKSDVTSGVSSFKDQMALNDDRILDIVHEPWWGEPTAVMMT
EEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
VFPSVIFQQQVNSVSTRHIQPNGPDSFDFVWTHFGFDDDTDEMTRRRLRQANLFGPAGFV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
SADDGEAIECSQEGFSQKPWHRVIAELGGKTAENTDHMVTETLIRGMYAYWRKVMEV
CCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8890734 [H]