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The map label for this gene is gntR [H]

Identifier: 73538469

GI number: 73538469

Start: 1328579

End: 1329574

Strand: Reverse

Name: gntR [H]

Synonym: Reut_B4642

Alternate gene names: 73538469

Gene position: 1329574-1328579 (Counterclockwise)

Preceding gene: 73538472

Following gene: 73538466

Centisome position: 48.77

GC content: 65.96

Gene sequence:

>996_bases
ATGTCGAATACCACTGGGCCGAAATCCGTGACGCTGCACGACGTGGCACGTGAGGCTGGCGTCTCACTGATTACCGCCTC
GCGTGCGCTTAGCAATCCCGGCATGGTGTCGGAGAAAACCATTGCGCGGGTCCGGCAGGCCGTCGAGGCGACTGGCTATA
TCCCGAACCTGCTTGCCGGCGGGCTCAAGTCGAAGCGCAGCCTGATGGTCGCCGCGCTCGTCCCGAACATTGCGGTGGCC
CAGTTCCTGCCGACGGTCAAGGCACTGACCGATGCGCTCGACGCGGCAGGCTATCAGGTGATCCTTGGCCAGACAGGCTA
CGACCACGCGCGCGAAGACGCGTTGCTCGCCACGATGATCAGCCGGCGTCCGGACGGCATCGTCGTCACCGGCCTGGTCC
ATTCGCAGGTGTCGCGGGAGCGCTTGCGGCGGGCGGGTATTCCTGTCGTGGAGACCTGGGATCTCAGCGACCGGCCCGTC
GATATGGTGGTCGGCTTTTCACACCTTAAGGTGGGTAGCGCCGTCGCGGGTTATTTCCTCGGCAAAGGATGGCGCCGCCT
CGGCATTGCAACCGGCGACGATCATCGCGCGTCGGTGCGGCGCGAGGGCTTTGTCTCGACGGTCGGCCACGACGTACCGA
CTGTGGTCGTGCCCGCGCCGAGCAGTCTGGCGCTGGGGCGCCGGGCCCTTGCCGATCTCCTCGAGAAAGATCCGCGGATC
GAAGCCGTCTATTGCAGCTCCGACCAGCTCGCGCAGGGCGTCATGGTCGAGGCGCTCGCACGCGGACTGCGCATTCCCGG
CGACCTGGCGATCTGTGGCTTCGGCGATGCCGATTTTGCCGCGCACCTGAATCCGTCGCTGACGACGGTACATGTGGATG
GCGCGGCCATTGGGGCGATGGCCGCTCGCTTGCTGCTGGAGCGCTGCCGTGGCGAGTCGGTTCCAGAACCGGTCGTCGAT
GTCGGATTCCGCATCGTCGAACGCGGTTCGACCTGA

Upstream 100 bases:

>100_bases
GCTGACGAAGGAATGATAGAAATGCTAACGTTGTCATTCTCGATCGTCAATCGCCTTCGGGCGAGCAACCGGTAGCGAAT
TACCCTGACTCAGGATCCGC

Downstream 100 bases:

>100_bases
CCTGGCCTTCACTCCAGCTTGATCCCCAGTTCCCGAATCAGCTTCGCATAGCGCGTCGTATCCCGCGCGATCAACGCGGC
AAACGCCTCCGGCGTGCTGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSNTTGPKSVTLHDVAREAGVSLITASRALSNPGMVSEKTIARVRQAVEATGYIPNLLAGGLKSKRSLMVAALVPNIAVA
QFLPTVKALTDALDAAGYQVILGQTGYDHAREDALLATMISRRPDGIVVTGLVHSQVSRERLRRAGIPVVETWDLSDRPV
DMVVGFSHLKVGSAVAGYFLGKGWRRLGIATGDDHRASVRREGFVSTVGHDVPTVVVPAPSSLALGRRALADLLEKDPRI
EAVYCSSDQLAQGVMVEALARGLRIPGDLAICGFGDADFAAHLNPSLTTVHVDGAAIGAMAARLLLERCRGESVPEPVVD
VGFRIVERGST

Sequences:

>Translated_331_residues
MSNTTGPKSVTLHDVAREAGVSLITASRALSNPGMVSEKTIARVRQAVEATGYIPNLLAGGLKSKRSLMVAALVPNIAVA
QFLPTVKALTDALDAAGYQVILGQTGYDHAREDALLATMISRRPDGIVVTGLVHSQVSRERLRRAGIPVVETWDLSDRPV
DMVVGFSHLKVGSAVAGYFLGKGWRRLGIATGDDHRASVRREGFVSTVGHDVPTVVVPAPSSLALGRRALADLLEKDPRI
EAVYCSSDQLAQGVMVEALARGLRIPGDLAICGFGDADFAAHLNPSLTTVHVDGAAIGAMAARLLLERCRGESVPEPVVD
VGFRIVERGST
>Mature_330_residues
SNTTGPKSVTLHDVAREAGVSLITASRALSNPGMVSEKTIARVRQAVEATGYIPNLLAGGLKSKRSLMVAALVPNIAVAQ
FLPTVKALTDALDAAGYQVILGQTGYDHAREDALLATMISRRPDGIVVTGLVHSQVSRERLRRAGIPVVETWDLSDRPVD
MVVGFSHLKVGSAVAGYFLGKGWRRLGIATGDDHRASVRREGFVSTVGHDVPTVVVPAPSSLALGRRALADLLEKDPRIE
AVYCSSDQLAQGVMVEALARGLRIPGDLAICGFGDADFAAHLNPSLTTVHVDGAAIGAMAARLLLERCRGESVPEPVVDV
GFRIVERGST

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=327, Percent_Identity=30.5810397553517, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1790715, Length=321, Percent_Identity=29.2834890965732, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=27.6595744680851, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=28.7878787878788, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=27.4626865671642, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788474, Length=325, Percent_Identity=27.0769230769231, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786540, Length=317, Percent_Identity=25.5520504731861, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787906, Length=263, Percent_Identity=27.3764258555133, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=23.5294117647059, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34952; Mature: 34820

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTTGPKSVTLHDVAREAGVSLITASRALSNPGMVSEKTIARVRQAVEATGYIPNLLAG
CCCCCCCCCCHHHHHHHHHCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHC
GLKSKRSLMVAALVPNIAVAQFLPTVKALTDALDAAGYQVILGQTGYDHAREDALLATMI
CCCHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHH
SRRPDGIVVTGLVHSQVSRERLRRAGIPVVETWDLSDRPVDMVVGFSHLKVGSAVAGYFL
HCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEECHHHHHHHHHHHHHHH
GKGWRRLGIATGDDHRASVRREGFVSTVGHDVPTVVVPAPSSLALGRRALADLLEKDPRI
HCCHHEECCCCCCCHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCE
EAVYCSSDQLAQGVMVEALARGLRIPGDLAICGFGDADFAAHLNPSLTTVHVDGAAIGAM
EEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHEECCCCEEEEEECCHHHHHH
AARLLLERCRGESVPEPVVDVGFRIVERGST
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNTTGPKSVTLHDVAREAGVSLITASRALSNPGMVSEKTIARVRQAVEATGYIPNLLAG
CCCCCCCCCHHHHHHHHHCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHC
GLKSKRSLMVAALVPNIAVAQFLPTVKALTDALDAAGYQVILGQTGYDHAREDALLATMI
CCCHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHH
SRRPDGIVVTGLVHSQVSRERLRRAGIPVVETWDLSDRPVDMVVGFSHLKVGSAVAGYFL
HCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEECHHHHHHHHHHHHHHH
GKGWRRLGIATGDDHRASVRREGFVSTVGHDVPTVVVPAPSSLALGRRALADLLEKDPRI
HCCHHEECCCCCCCHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCE
EAVYCSSDQLAQGVMVEALARGLRIPGDLAICGFGDADFAAHLNPSLTTVHVDGAAIGAM
EEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHEECCCCEEEEEECCHHHHHH
AARLLLERCRGESVPEPVVDVGFRIVERGST
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]