BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is crt [H]

Identifier: 73538424

GI number: 73538424

Start: 1282789

End: 1283601

Strand: Reverse

Name: crt [H]

Synonym: Reut_B4597

Alternate gene names: 73538424

Gene position: 1283601-1282789 (Counterclockwise)

Preceding gene: 73538425

Following gene: 73538423

Centisome position: 47.08

GC content: 66.05

Gene sequence:

>813_bases
ATGAGCGAATACCAACTGATCCATCTCGAAGACCGTGCAGGCATCGCAGTCCTGACGCTGAACCGTCCTGAAAAGCGCAA
CGCCATCAGCGACGACATGCGCACTGAGCTCATCCATGCGCTCGAGTCCATCGCCGCCAGCCGCGAAATCCGCGCGCTCG
TGCTGACCGGCAACGGCAAAGGCTTCTGCGCCGGCGGGGACGTAGCGGGCATGCAGCGGCGCATGGATGCGCCGGCTGGC
GAAGTCGGCTTCAACGGCTGGACGCGCCAGCAGCGCGTGCACCACTCGGTGGCGCTGCTGCACGATATGCCCAAGCCGGT
CATCGCGGCGGTCAATGGCAGCGCCAACGGACTCGGCGCCGACCTGGCCATGGCCTGCGACTTCGTGATGGCCTCCGCAT
CGGCCAGCTTTGCCTGGTCCTACATCCATCGCGGGCTGATTCCGGATGGCGGCGGCATGTACTTCCTGCCGCGCCGAACC
GGACTGTCCATCGCCAAGGGGCTGATCTTCAGCGGCCGCAAGGTAGAGGCCGATGAAGCGCTGACCCTGGGCATCGCAGA
CCGGCTGAGCACGTCGCCGGAAGCGCTGGTGTCCGAAGCGGTCGCGTGGGCGGCCGAGTTGTCGCAGGGCTCGGCCACGG
CGCTGGCGCTGGGCAAGACGATCCTGAACCAGACCTTCGAGCTCACCGCGGATCAAGTGTTCGCGCAGGGCAGCCAGGCC
CAAGGCATTTGCTACACGAGCGCCGAGCATCGCGAATCCGTGCTCGCCTTCCTTGCGAAGACAGCCACTCCAAACGCAGC
GGCGAAGGGCTAA

Upstream 100 bases:

>100_bases
CGCTACGTGCTCGGAGAGCAGGCGCGCTGGAGCAAGGTCGTCAAGGACGCCCACATCACCCTTAACTGACATCCTCCGCA
ACATCATCCAGGCACCCATC

Downstream 100 bases:

>100_bases
GACATCATGACGACATCCCATCAGTTTTCGCCGGCTGCCACGCGCCCGAATGCCGACGCGATCTCGCGCCTCTTCACGCC
ACGAAGCGTGGCGGTGATCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSEYQLIHLEDRAGIAVLTLNRPEKRNAISDDMRTELIHALESIAASREIRALVLTGNGKGFCAGGDVAGMQRRMDAPAG
EVGFNGWTRQQRVHHSVALLHDMPKPVIAAVNGSANGLGADLAMACDFVMASASASFAWSYIHRGLIPDGGGMYFLPRRT
GLSIAKGLIFSGRKVEADEALTLGIADRLSTSPEALVSEAVAWAAELSQGSATALALGKTILNQTFELTADQVFAQGSQA
QGICYTSAEHRESVLAFLAKTATPNAAAKG

Sequences:

>Translated_270_residues
MSEYQLIHLEDRAGIAVLTLNRPEKRNAISDDMRTELIHALESIAASREIRALVLTGNGKGFCAGGDVAGMQRRMDAPAG
EVGFNGWTRQQRVHHSVALLHDMPKPVIAAVNGSANGLGADLAMACDFVMASASASFAWSYIHRGLIPDGGGMYFLPRRT
GLSIAKGLIFSGRKVEADEALTLGIADRLSTSPEALVSEAVAWAAELSQGSATALALGKTILNQTFELTADQVFAQGSQA
QGICYTSAEHRESVLAFLAKTATPNAAAKG
>Mature_269_residues
SEYQLIHLEDRAGIAVLTLNRPEKRNAISDDMRTELIHALESIAASREIRALVLTGNGKGFCAGGDVAGMQRRMDAPAGE
VGFNGWTRQQRVHHSVALLHDMPKPVIAAVNGSANGLGADLAMACDFVMASASASFAWSYIHRGLIPDGGGMYFLPRRTG
LSIAKGLIFSGRKVEADEALTLGIADRLSTSPEALVSEAVAWAAELSQGSATALALGKTILNQTFELTADQVFAQGSQAQ
GICYTSAEHRESVLAFLAKTATPNAAAKG

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=266, Percent_Identity=31.9548872180451, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI70995211, Length=210, Percent_Identity=35.2380952380952, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI68989263, Length=208, Percent_Identity=34.1346153846154, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4502327, Length=267, Percent_Identity=29.5880149812734, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI260274832, Length=262, Percent_Identity=27.8625954198473, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI45643119, Length=262, Percent_Identity=27.8625954198473, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI260275230, Length=262, Percent_Identity=27.8625954198473, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI221136756, Length=258, Percent_Identity=25.5813953488372, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136753, Length=258, Percent_Identity=25.5813953488372, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4757968, Length=256, Percent_Identity=25, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI49355787, Length=256, Percent_Identity=25, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221307494, Length=258, Percent_Identity=25.5813953488372, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI116235487, Length=243, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=262, Percent_Identity=24.8091603053435, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI51538573, Length=262, Percent_Identity=24.8091603053435, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=259, Percent_Identity=25.0965250965251, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538581, Length=259, Percent_Identity=25.0965250965251, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=28.9617486338798, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI37594471, Length=185, Percent_Identity=28.6486486486486, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=38.0769230769231, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=32.8063241106719, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=32.5396825396825, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788597, Length=217, Percent_Identity=34.1013824884793, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=26.2172284644195, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788682, Length=212, Percent_Identity=28.3018867924528, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790281, Length=238, Percent_Identity=26.890756302521, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=30.7359307359307, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=32, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=247, Percent_Identity=27.1255060728745, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=185, Percent_Identity=31.3513513513514, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=27.6190476190476, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=264, Percent_Identity=25.7575757575758, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=183, Percent_Identity=30.0546448087432, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=183, Percent_Identity=30.0546448087432, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=229, Percent_Identity=24.8908296943231, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=25, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=227, Percent_Identity=27.3127753303965, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=187, Percent_Identity=28.3422459893048, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320241, Length=196, Percent_Identity=28.0612244897959, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=32.7935222672065, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=32.7935222672065, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=30.6772908366534, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=28.7937743190661, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24654903, Length=227, Percent_Identity=25.9911894273128, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21357171, Length=272, Percent_Identity=27.9411764705882, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=31.9767441860465, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=31.9767441860465, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922422, Length=268, Percent_Identity=22.0149253731343, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28482; Mature: 28350

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYQLIHLEDRAGIAVLTLNRPEKRNAISDDMRTELIHALESIAASREIRALVLTGNGK
CCCEEEEEEECCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
GFCAGGDVAGMQRRMDAPAGEVGFNGWTRQQRVHHSVALLHDMPKPVIAAVNGSANGLGA
EEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCH
DLAMACDFVMASASASFAWSYIHRGLIPDGGGMYFLPRRTGLSIAKGLIFSGRKVEADEA
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEECCCCE
LTLGIADRLSTSPEALVSEAVAWAAELSQGSATALALGKTILNQTFELTADQVFAQGSQA
EEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QGICYTSAEHRESVLAFLAKTATPNAAAKG
CCEEEECHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEYQLIHLEDRAGIAVLTLNRPEKRNAISDDMRTELIHALESIAASREIRALVLTGNGK
CCEEEEEEECCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
GFCAGGDVAGMQRRMDAPAGEVGFNGWTRQQRVHHSVALLHDMPKPVIAAVNGSANGLGA
EEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCH
DLAMACDFVMASASASFAWSYIHRGLIPDGGGMYFLPRRTGLSIAKGLIFSGRKVEADEA
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEECCCCE
LTLGIADRLSTSPEALVSEAVAWAAELSQGSATALALGKTILNQTFELTADQVFAQGSQA
EEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QGICYTSAEHRESVLAFLAKTATPNAAAKG
CCEEEECHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]