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The map label for this gene is alsR [H]

Identifier: 73538382

GI number: 73538382

Start: 1232610

End: 1233509

Strand: Direct

Name: alsR [H]

Synonym: Reut_B4555

Alternate gene names: 73538382

Gene position: 1232610-1233509 (Clockwise)

Preceding gene: 73538381

Following gene: 73538392

Centisome position: 45.21

GC content: 65.67

Gene sequence:

>900_bases
ATGGATCTCCGCCAGATGAAGCAGGCCCTGGTGCTGAGCGAGACACTCAACTTCCACCGGGCCGCCGAACGGCTGCACAT
GGCGCAGCCACCACTCTCCACTGCCATCCGCAAGCTGGAAGAAGAACTGGGTGTCACCCTGTTCGACCGCCAGCCATCCG
GCTTGTCGCTCACGTCCGCGGGCGAGGTGGTCCTGCGGCAGATGCGCAGTACGCTCTTCTTCGCCGATGAAATCCGCCGC
GCAGCGCGTGAGGGCGAATGTGGCGAACAGGGTACGTTGCGCGTGGGATTCGTTGGCTCGTCGGTCTACTCGGTCATGCC
CCAACTGATCCGTGCATTTCGTCTCAAGTATCCGCGAGTCGAACTGATCATCGAGGAATCGACGACCGTCGATCTGCTGC
GCGCGGTCGAGGAACACTCGCTCGACGTGGCGCTCGTGCGCTACCCCGTGCTCGCGCAGACCAGTGCGCAGATCACCTTT
CTGCGCCCTGACCCACTGGTGCTGGCCGTCAGCGCTGATTCGGCACTGGCCGGTCGGTCAAGCATCGACCTGTCGGAACT
GGGCACGACGCCCTTCATTGTCTACTCGGAGTCGCGCGTCCCGGCCATGCATGCGATGACGATGTCGGCTTTCCACCAAG
CCGGCATCCGCCCGCCGATCGCGCAGCAAGCCGTGCAGGTGCAGACGTTGCTCGCGCTGGTCGAAAGCGGGCTGGGGGTA
GCGCTGGTGCCCGCCACGGCGGCGGGCTACGCGGGGAACGGGACTAGGCTGGTGCCACTGACGGGCCTGCCGGACAAGCT
GAACTTCGGGCTGGCGCTGGCGACGCTGGACGATACCAGCATCGCCACGACACGCAACTTCGTGGCGCTGGCGCAGGCCG
TCACGCGGCCCGCTTCCTGA

Upstream 100 bases:

>100_bases
CGGCCTTTTGCTTTGCACGGCCGGACAATCCCTGATTGTTTAATCCGACCGTTCGGAGTAACCTACCGCCCAACGTAATA
CAAGAACACGGAGTACGGAG

Downstream 100 bases:

>100_bases
CGGATCAGCGCTTCGCCGCGCGCCGCGAGATCAGCGCATCCACGGCCACGATGCGGTCGGCCGTGCAGATCAGCGGGTTG
ACGTCAAACTCTTCAATCGC

Product: regulatory protein LysR

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDLRQMKQALVLSETLNFHRAAERLHMAQPPLSTAIRKLEEELGVTLFDRQPSGLSLTSAGEVVLRQMRSTLFFADEIRR
AAREGECGEQGTLRVGFVGSSVYSVMPQLIRAFRLKYPRVELIIEESTTVDLLRAVEEHSLDVALVRYPVLAQTSAQITF
LRPDPLVLAVSADSALAGRSSIDLSELGTTPFIVYSESRVPAMHAMTMSAFHQAGIRPPIAQQAVQVQTLLALVESGLGV
ALVPATAAGYAGNGTRLVPLTGLPDKLNFGLALATLDDTSIATTRNFVALAQAVTRPAS

Sequences:

>Translated_299_residues
MDLRQMKQALVLSETLNFHRAAERLHMAQPPLSTAIRKLEEELGVTLFDRQPSGLSLTSAGEVVLRQMRSTLFFADEIRR
AAREGECGEQGTLRVGFVGSSVYSVMPQLIRAFRLKYPRVELIIEESTTVDLLRAVEEHSLDVALVRYPVLAQTSAQITF
LRPDPLVLAVSADSALAGRSSIDLSELGTTPFIVYSESRVPAMHAMTMSAFHQAGIRPPIAQQAVQVQTLLALVESGLGV
ALVPATAAGYAGNGTRLVPLTGLPDKLNFGLALATLDDTSIATTRNFVALAQAVTRPAS
>Mature_299_residues
MDLRQMKQALVLSETLNFHRAAERLHMAQPPLSTAIRKLEEELGVTLFDRQPSGLSLTSAGEVVLRQMRSTLFFADEIRR
AAREGECGEQGTLRVGFVGSSVYSVMPQLIRAFRLKYPRVELIIEESTTVDLLRAVEEHSLDVALVRYPVLAQTSAQITF
LRPDPLVLAVSADSALAGRSSIDLSELGTTPFIVYSESRVPAMHAMTMSAFHQAGIRPPIAQQAVQVQTLLALVESGLGV
ALVPATAAGYAGNGTRLVPLTGLPDKLNFGLALATLDDTSIATTRNFVALAQAVTRPAS

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=298, Percent_Identity=31.2080536912752, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787879, Length=261, Percent_Identity=29.8850574712644, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=27.7566539923954, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=27.710843373494, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787530, Length=260, Percent_Identity=28.0769230769231, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693105, Length=253, Percent_Identity=27.6679841897233, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787806, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=249, Percent_Identity=24.8995983935743, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=26.4, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=27.2357723577236, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=32.8467153284672, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786401, Length=159, Percent_Identity=36.4779874213836, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32265; Mature: 32265

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQMKQALVLSETLNFHRAAERLHMAQPPLSTAIRKLEEELGVTLFDRQPSGLSLTSA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCEECHH
GEVVLRQMRSTLFFADEIRRAAREGECGEQGTLRVGFVGSSVYSVMPQLIRAFRLKYPRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCE
ELIIEESTTVDLLRAVEEHSLDVALVRYPVLAQTSAQITFLRPDPLVLAVSADSALAGRS
EEEEECCHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEECCCCEEEEEECCCCCCCCC
SIDLSELGTTPFIVYSESRVPAMHAMTMSAFHQAGIRPPIAQQAVQVQTLLALVESGLGV
CCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
ALVPATAAGYAGNGTRLVPLTGLPDKLNFGLALATLDDTSIATTRNFVALAQAVTRPAS
EEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLRQMKQALVLSETLNFHRAAERLHMAQPPLSTAIRKLEEELGVTLFDRQPSGLSLTSA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCEECHH
GEVVLRQMRSTLFFADEIRRAAREGECGEQGTLRVGFVGSSVYSVMPQLIRAFRLKYPRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCE
ELIIEESTTVDLLRAVEEHSLDVALVRYPVLAQTSAQITFLRPDPLVLAVSADSALAGRS
EEEEECCHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEECCCCEEEEEECCCCCCCCC
SIDLSELGTTPFIVYSESRVPAMHAMTMSAFHQAGIRPPIAQQAVQVQTLLALVESGLGV
CCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
ALVPATAAGYAGNGTRLVPLTGLPDKLNFGLALATLDDTSIATTRNFVALAQAVTRPAS
EEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]