BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is crt [H]

Identifier: 73538380

GI number: 73538380

Start: 1230912

End: 1231697

Strand: Direct

Name: crt [H]

Synonym: Reut_B4553

Alternate gene names: 73538380

Gene position: 1230912-1231697 (Clockwise)

Preceding gene: 73538379

Following gene: 73538381

Centisome position: 45.15

GC content: 67.18

Gene sequence:

>786_bases
ATGAGCAATTTTCTTGATATCCGCCGCGATGGCCATGTCGCCATCGTCACCATGAACCGCCCGGAGACGCGCAACGCGGT
CTCCGACAACGACGCCGTCGATGCACTGGTCGCCATGTGCGACGCGTTCAACCGCGACCATTCCGTACGCGTCGTCGTGC
TGACGGGCGCCGGCACAGCGTTCTCGTCTGGCGGCAACCTCAAGACGCTGCGTGCGACGTTCGGCAGCGAATCCGGTGAG
CCGGTCAGTGCACGCTACGCCTATCGCGAAGGCATTCAGCGCCTGCCGCTCGCGGTGGCCAACCTCGAAGTGCCGGTGAT
TGCCGCGGTGAACGGCCCGGCCCTCGGTGCCGGCAACGACCTGGCCTGCACCTGCGACATCCGCATCGCTTCCGAGAAGG
CCGTCTTCGCCGCCACGTTCGTGAAGCTGGGCCTGATCCCCGGCGACGGCGGCGCATGGCTGCTGCCGCGTGCCATCGGC
GCATCGCGCGCGGCCGAGCTGTGCTTCACCGGCGACACCATCGACGCCGCCACGGCGCGCGAGTACGGGCTGGTTTCGCG
CGTGGTACCGCACGAGGAACTGCTGCCGACCGCGCTGGAACTCGCGCACCGCATCGCCGCCAATCCGGGGCATGCGCTGC
GCATGTGCAAGCGCCTGCTCAAAGAAGCCCAGCATGCGCGGCTCGACACCATTCTCGAAATGTCGGCCGGGTTCCAGGCG
CTGGCGCACCATACCGCCGAACACGAAGCCGCACTCGATGGCTTCCTGGCATGCATCAAAGGCTGA

Upstream 100 bases:

>100_bases
GGGCTGGCTGCCTGCCGCGCGGATTGCCGAAGTGCGCGCGCTCGCCGAGAAAGCCGACCGGGACTGAATACCGGCCAACC
TGTTTTCACGACCAAAGACC

Downstream 100 bases:

>100_bases
GGAACGCCATGGAAATTCGCAATAACGTCTTCATTGTCACCGGCGGCGCGTCGGGACTTGGCGAGGCCACGGCCCGAATG
CTGGCCGCCGCCGGAGGCAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSNFLDIRRDGHVAIVTMNRPETRNAVSDNDAVDALVAMCDAFNRDHSVRVVVLTGAGTAFSSGGNLKTLRATFGSESGE
PVSARYAYREGIQRLPLAVANLEVPVIAAVNGPALGAGNDLACTCDIRIASEKAVFAATFVKLGLIPGDGGAWLLPRAIG
ASRAAELCFTGDTIDAATAREYGLVSRVVPHEELLPTALELAHRIAANPGHALRMCKRLLKEAQHARLDTILEMSAGFQA
LAHHTAEHEAALDGFLACIKG

Sequences:

>Translated_261_residues
MSNFLDIRRDGHVAIVTMNRPETRNAVSDNDAVDALVAMCDAFNRDHSVRVVVLTGAGTAFSSGGNLKTLRATFGSESGE
PVSARYAYREGIQRLPLAVANLEVPVIAAVNGPALGAGNDLACTCDIRIASEKAVFAATFVKLGLIPGDGGAWLLPRAIG
ASRAAELCFTGDTIDAATAREYGLVSRVVPHEELLPTALELAHRIAANPGHALRMCKRLLKEAQHARLDTILEMSAGFQA
LAHHTAEHEAALDGFLACIKG
>Mature_260_residues
SNFLDIRRDGHVAIVTMNRPETRNAVSDNDAVDALVAMCDAFNRDHSVRVVVLTGAGTAFSSGGNLKTLRATFGSESGEP
VSARYAYREGIQRLPLAVANLEVPVIAAVNGPALGAGNDLACTCDIRIASEKAVFAATFVKLGLIPGDGGAWLLPRAIGA
SRAAELCFTGDTIDAATAREYGLVSRVVPHEELLPTALELAHRIAANPGHALRMCKRLLKEAQHARLDTILEMSAGFQAL
AHHTAEHEAALDGFLACIKG

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=209, Percent_Identity=36.8421052631579, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI31542718, Length=202, Percent_Identity=32.6732673267327, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI70995211, Length=190, Percent_Identity=36.3157894736842, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=32.8, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI68989263, Length=225, Percent_Identity=32, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI116235487, Length=188, Percent_Identity=30.3191489361702, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260274832, Length=224, Percent_Identity=27.2321428571429, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=224, Percent_Identity=27.2321428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=224, Percent_Identity=27.2321428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594469, Length=190, Percent_Identity=27.3684210526316, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594471, Length=190, Percent_Identity=27.3684210526316, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=29.1208791208791, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI25453481, Length=231, Percent_Identity=27.7056277056277, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538573, Length=231, Percent_Identity=27.7056277056277, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757966, Length=231, Percent_Identity=27.7056277056277, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI51538581, Length=231, Percent_Identity=27.7056277056277, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4757968, Length=221, Percent_Identity=25.7918552036199, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI49355787, Length=221, Percent_Identity=25.7918552036199, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI157694516, Length=215, Percent_Identity=25.1162790697674, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI213417737, Length=215, Percent_Identity=25.1162790697674, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=37.8378378378378, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=33.4630350194552, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=32.258064516129, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI221142681, Length=222, Percent_Identity=32.8828828828829, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=32.512315270936, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=25.4826254826255, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=34.4262295081967, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=221, Percent_Identity=34.841628959276, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=208, Percent_Identity=29.8076923076923, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17535521, Length=224, Percent_Identity=29.0178571428571, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=202, Percent_Identity=29.2079207920792, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17508953, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=182, Percent_Identity=32.967032967033, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=233, Percent_Identity=28.3261802575107, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=29.7435897435897, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=195, Percent_Identity=29.7435897435897, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=227, Percent_Identity=25.5506607929515, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=187, Percent_Identity=25.1336898395722, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=33.6, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=33.6, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24654903, Length=219, Percent_Identity=28.310502283105, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653139, Length=231, Percent_Identity=32.4675324675325, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=33.0188679245283, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24650670, Length=247, Percent_Identity=28.7449392712551, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920382, Length=213, Percent_Identity=32.3943661971831, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=215, Percent_Identity=28.3720930232558, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27562; Mature: 27431

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFLDIRRDGHVAIVTMNRPETRNAVSDNDAVDALVAMCDAFNRDHSVRVVVLTGAGTA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
FSSGGNLKTLRATFGSESGEPVSARYAYREGIQRLPLAVANLEVPVIAAVNGPALGAGND
ECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEEEECCCCCCCCCC
LACTCDIRIASEKAVFAATFVKLGLIPGDGGAWLLPRAIGASRAAELCFTGDTIDAATAR
EEEEEEEEECCCCHHHHHHHHHHEEEECCCCCEEHHHHHCCHHHHHEEECCCCCCHHHHH
EYGLVSRVVPHEELLPTALELAHRIAANPGHALRMCKRLLKEAQHARLDTILEMSAGFQA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHHTAEHEAALDGFLACIKG
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNFLDIRRDGHVAIVTMNRPETRNAVSDNDAVDALVAMCDAFNRDHSVRVVVLTGAGTA
CCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
FSSGGNLKTLRATFGSESGEPVSARYAYREGIQRLPLAVANLEVPVIAAVNGPALGAGND
ECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEEEECCCCCCCCCC
LACTCDIRIASEKAVFAATFVKLGLIPGDGGAWLLPRAIGASRAAELCFTGDTIDAATAR
EEEEEEEEECCCCHHHHHHHHHHEEEECCCCCEEHHHHHCCHHHHHEEECCCCCCHHHHH
EYGLVSRVVPHEELLPTALELAHRIAANPGHALRMCKRLLKEAQHARLDTILEMSAGFQA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAHHTAEHEAALDGFLACIKG
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]