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The map label for this gene is 73538362

Identifier: 73538362

GI number: 73538362

Start: 1208791

End: 1209735

Strand: Direct

Name: 73538362

Synonym: Reut_B4535

Alternate gene names: NA

Gene position: 1208791-1209735 (Clockwise)

Preceding gene: 73538361

Following gene: 73538363

Centisome position: 44.34

GC content: 66.46

Gene sequence:

>945_bases
ATGTCCCGCTTTGTCCACGTCGCCGCAGCGCAGCTCGGCCCCATCCAGCGCAGCGATTCGCGCCGCGATGTCGTCGCCCG
CCTGCTGGCCCACCTGCACCACGCCCATGAGATGGGCTGCCATCTGGTGGTCTTTCCCGAACTGGCGCTGACCACCTTCT
TTCCGCGCTGGTACATGGATGATCCCGAGGAACTGAATGCGTTCTACGAGCGCGAAATGCCCGGCCCGGAAACCCGCATC
CTGTTCGACACGGCAGCACGGCTCGGCATCGGCTTCTATCTCGGCTACGCGGAACTCGCGGTCGAGCAAGGCGTCGAGCG
CCGCTACAACACCGCGATCCTGGTCGGCCGCGACGGCAAGATCGTCGGCAAGTACCGCAAGGTGCATCTGCCCGGCCATG
ACCAGCACGAACCGTGGCGCGCCTTCCAGCACCTGGAAAAGCGCTACTTCGAGCCCGGCGACGACTTCGGCGTCTGGCAG
GCCTTCGGCGGCGTGATGGGCATGGCGCTGTGCAACGACCGCCGCTGGTCCGAGACCTATCGCGTGATGGGCCTGCAAGG
CGCGGAAATGATCCTGCTCGGCTACAACACACCGGTTCACAACCCGCCGGCGCCCGAGCACGACGACCTGTCGATGTTCC
ACAACCACCTGGTCATGCAAGCCGGCGCGTACCAGAACGGCACATGGGTGGTCGGCGTGGCAAAGGCCGGCAAGGAAGAA
GGCTGCGAGATGATCGGCGGCACCAGCATCATCGCGCCGTCCGGCGAGATCGTCGCGGCTTGCTCGACCAAGGGCGATGA
ACTGGCAATCGCCCGCTGCGACCTGGACCTGTGCCAGTCGTACAAGAGCACGACCTTCAACTTCGACCGGCACCGCCGCC
CCGAGGCCTACGGCCGCATCACGAGCCAGCGCGGCGCCACGCCGCCGCAGCCCGTGGCCGCATAG

Upstream 100 bases:

>100_bases
GACCCACGACCACGACATCCAGGCGGGCGCCGACGTGCTGCTCGACCTGATGCTCGAACTGGCCGCCTGACATTCACCGC
ATAACAGGAGACAACCCACC

Downstream 100 bases:

>100_bases
CCGCACGCGCTAGTCGCCCCTCTCCAAATCTAGAAAGAAGAACACGATGCAGGCGCCTCCGGGCGCCCGCAGGGGGGCGT
TTTTGTCTCACCAAATCCAA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSRFVHVAAAQLGPIQRSDSRRDVVARLLAHLHHAHEMGCHLVVFPELALTTFFPRWYMDDPEELNAFYEREMPGPETRI
LFDTAARLGIGFYLGYAELAVEQGVERRYNTAILVGRDGKIVGKYRKVHLPGHDQHEPWRAFQHLEKRYFEPGDDFGVWQ
AFGGVMGMALCNDRRWSETYRVMGLQGAEMILLGYNTPVHNPPAPEHDDLSMFHNHLVMQAGAYQNGTWVVGVAKAGKEE
GCEMIGGTSIIAPSGEIVAACSTKGDELAIARCDLDLCQSYKSTTFNFDRHRRPEAYGRITSQRGATPPQPVAA

Sequences:

>Translated_314_residues
MSRFVHVAAAQLGPIQRSDSRRDVVARLLAHLHHAHEMGCHLVVFPELALTTFFPRWYMDDPEELNAFYEREMPGPETRI
LFDTAARLGIGFYLGYAELAVEQGVERRYNTAILVGRDGKIVGKYRKVHLPGHDQHEPWRAFQHLEKRYFEPGDDFGVWQ
AFGGVMGMALCNDRRWSETYRVMGLQGAEMILLGYNTPVHNPPAPEHDDLSMFHNHLVMQAGAYQNGTWVVGVAKAGKEE
GCEMIGGTSIIAPSGEIVAACSTKGDELAIARCDLDLCQSYKSTTFNFDRHRRPEAYGRITSQRGATPPQPVAA
>Mature_313_residues
SRFVHVAAAQLGPIQRSDSRRDVVARLLAHLHHAHEMGCHLVVFPELALTTFFPRWYMDDPEELNAFYEREMPGPETRIL
FDTAARLGIGFYLGYAELAVEQGVERRYNTAILVGRDGKIVGKYRKVHLPGHDQHEPWRAFQHLEKRYFEPGDDFGVWQA
FGGVMGMALCNDRRWSETYRVMGLQGAEMILLGYNTPVHNPPAPEHDDLSMFHNHLVMQAGAYQNGTWVVGVAKAGKEEG
CEMIGGTSIIAPSGEIVAACSTKGDELAIARCDLDLCQSYKSTTFNFDRHRRPEAYGRITSQRGATPPQPVAA

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=265, Percent_Identity=25.6603773584906, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=264, Percent_Identity=25, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 35185; Mature: 35054

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFVHVAAAQLGPIQRSDSRRDVVARLLAHLHHAHEMGCHLVVFPELALTTFFPRWYMD
CCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHCCC
DPEELNAFYEREMPGPETRILFDTAARLGIGFYLGYAELAVEQGVERRYNTAILVGRDGK
CHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
IVGKYRKVHLPGHDQHEPWRAFQHLEKRYFEPGDDFGVWQAFGGVMGMALCNDRRWSETY
EEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RVMGLQGAEMILLGYNTPVHNPPAPEHDDLSMFHNHLVMQAGAYQNGTWVVGVAKAGKEE
HEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHH
GCEMIGGTSIIAPSGEIVAACSTKGDELAIARCDLDLCQSYKSTTFNFDRHRRPEAYGRI
CHHHCCCCEEECCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCHHCCCCHHHHCH
TSQRGATPPQPVAA
HHCCCCCCCCCCCC
>Mature Secondary Structure 
SRFVHVAAAQLGPIQRSDSRRDVVARLLAHLHHAHEMGCHLVVFPELALTTFFPRWYMD
CCEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCHHCCC
DPEELNAFYEREMPGPETRILFDTAARLGIGFYLGYAELAVEQGVERRYNTAILVGRDGK
CHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
IVGKYRKVHLPGHDQHEPWRAFQHLEKRYFEPGDDFGVWQAFGGVMGMALCNDRRWSETY
EEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RVMGLQGAEMILLGYNTPVHNPPAPEHDDLSMFHNHLVMQAGAYQNGTWVVGVAKAGKEE
HEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHH
GCEMIGGTSIIAPSGEIVAACSTKGDELAIARCDLDLCQSYKSTTFNFDRHRRPEAYGRI
CHHHCCCCEEECCCCCEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCHHCCCCHHHHCH
TSQRGATPPQPVAA
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]