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The map label for this gene is emrY [H]

Identifier: 73538315

GI number: 73538315

Start: 1158106

End: 1159731

Strand: Reverse

Name: emrY [H]

Synonym: Reut_B4488

Alternate gene names: 73538315

Gene position: 1159731-1158106 (Counterclockwise)

Preceding gene: 73538316

Following gene: 73538314

Centisome position: 42.54

GC content: 62.3

Gene sequence:

>1626_bases
ATGACCACAGCGAATCCTTCAACCACTGCAACGGATGTGCCGCCCGTCGAGCAACAGACGCGCCCGGTCTCCGTGCAGGA
GGAGGGGATCCCTTCGCATCCAAAGCCCGCGGGTGCCGCACTGTCGCTGCGCCTGGCGACCGGCCTGATCGGCGTCTTGC
TCGCATCGTTGCTGGCCATCCTCAATGAACAGGTGACCGCGCTGGCTTTGGCCGATGTACAGGGCGTGTTCTCGATCGGC
CGTGACGACGGGACGTGGCTCACGACGCTGTTCGAAGCGGCCAATGTCGCAACGATGGTATTTGCACCCTGGTTCGGCAT
TACCTTTACGCTGAAGCGATTCACGATCGGCGCCGTGCTGGCCACGATGCTGCTTGGCGTTCTTTGCCCGTTTGCGCCGA
GCCTCCAGGCGCTCTACCTGCTTCGCGTCCTGCAGGGTGTCGCGGGCGGCTGCCTGCCACCGATGCTGATCATCGTGGCA
TTGCGCTATCTTCCCCCGAAGATCAAGCTGTATGGACTCGCCGGCTACGCGTTGACTGCGACCTTCGGCCCCGCAGTCGG
TACGCCACTTGCCGCGTTGTGGACGGAGTACGTCAGCTGGGAGATGGTCTTCTGGCAGATCGTGCCACTCGGTATTGCGA
GCTGTGTGGCCGTCCGGTGGGGCCTGCCGGATGATCCGTACAAGCTTGAGCGGCTCAGTGCATTCAACTGGACCGGGTTG
CTGACCGGATTCCCCGCAGTCGCCATGCTCGTAACCGGCCTCCTGCAGGGGGACCGTCTCGACTGGCTGAGCTCCGAGTT
CATCCGCGTGATGTTCGGCGGCGGCGTAGTCCTTCTCACTGTGTTTCTCTTCAACGAGTGGTTTCACCCGCTTCCCTTCT
TCAAGCTTCAGTTGCTCGCCCGCAGGAACTTCGCGCATGGGCTCATGACGCTGGCGGGTGCCGTGATCCTGCTGGTCGGC
GTGGCAGCCATTCCCGGCCAATACCTCGCCAGGGTTCACGCATACAGGCCGCTGCAAACGGCACCGCTGTCGTTACTGCT
TGCGCTTCCGCTGTTGATCACACTGCCGCTGACGGCGGCCGTGCTGAACCTTCGGCGCGTGGATTCCCGGTGGGTGATGA
CGGCTGGGCTGCTGCTCATGATTGCCACGTGCGCGCTGGGAAGTCTTATGACTTCCGAATGGATACGCGTCAATTTCTAC
TGGCTTCAGGCGCTGCAGATCATGGCCCAGCCAATGGTGATCCTGTCGATCCTGATGGGCGTCACCACGGGATTGCCTCC
CACGGAGGGCCCGTTCGCCTCGGCCATGTTCAACTCGCTCAAGACGTTTTCTGCTGCCGTGGCAACGGCCCTGATCGAGG
GGCTGGGTACCGGTCGCGAACGCTTTCATTCGAACATGCTGGTGGACCAGCTTGGCAACAATGCCGTCGTGGCAGGCCGG
GGCGTCGATTCGGTGCACACCCTGGCTCAGCTCGCCCATCGTGTCCACGAGCAAGCGCTCGTACTGACCTCGGCCGATCT
GTACCGCGTGATGGCCTGCGTTGCCATTGCCTTGCTGTTTCTCGTTCCGATATTGCCCGTGCGCATCTATCCGCCCTGGA
GCACTACGCCACCCTCTTCACACTAA

Upstream 100 bases:

>100_bases
TTGAAGTCGCCCACCGCTTCTTCCAGAGATTGCCGGCGTGACCCGCCGCAGAAAGTGGAATCTCCGGAGCCAAGACCCAT
CAAGTCGTCAGGGGACATTT

Downstream 100 bases:

>100_bases
GGGAAGATCATCATGTCATCCGCTACCCGCCCGCCAATCAGGAATATCCGCCTTGCAGCGATCTTCATCGCGGTCGGCAT
CGTTGCGTGGGCCTGCGCAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MTTANPSTTATDVPPVEQQTRPVSVQEEGIPSHPKPAGAALSLRLATGLIGVLLASLLAILNEQVTALALADVQGVFSIG
RDDGTWLTTLFEAANVATMVFAPWFGITFTLKRFTIGAVLATMLLGVLCPFAPSLQALYLLRVLQGVAGGCLPPMLIIVA
LRYLPPKIKLYGLAGYALTATFGPAVGTPLAALWTEYVSWEMVFWQIVPLGIASCVAVRWGLPDDPYKLERLSAFNWTGL
LTGFPAVAMLVTGLLQGDRLDWLSSEFIRVMFGGGVVLLTVFLFNEWFHPLPFFKLQLLARRNFAHGLMTLAGAVILLVG
VAAIPGQYLARVHAYRPLQTAPLSLLLALPLLITLPLTAAVLNLRRVDSRWVMTAGLLLMIATCALGSLMTSEWIRVNFY
WLQALQIMAQPMVILSILMGVTTGLPPTEGPFASAMFNSLKTFSAAVATALIEGLGTGRERFHSNMLVDQLGNNAVVAGR
GVDSVHTLAQLAHRVHEQALVLTSADLYRVMACVAIALLFLVPILPVRIYPPWSTTPPSSH

Sequences:

>Translated_541_residues
MTTANPSTTATDVPPVEQQTRPVSVQEEGIPSHPKPAGAALSLRLATGLIGVLLASLLAILNEQVTALALADVQGVFSIG
RDDGTWLTTLFEAANVATMVFAPWFGITFTLKRFTIGAVLATMLLGVLCPFAPSLQALYLLRVLQGVAGGCLPPMLIIVA
LRYLPPKIKLYGLAGYALTATFGPAVGTPLAALWTEYVSWEMVFWQIVPLGIASCVAVRWGLPDDPYKLERLSAFNWTGL
LTGFPAVAMLVTGLLQGDRLDWLSSEFIRVMFGGGVVLLTVFLFNEWFHPLPFFKLQLLARRNFAHGLMTLAGAVILLVG
VAAIPGQYLARVHAYRPLQTAPLSLLLALPLLITLPLTAAVLNLRRVDSRWVMTAGLLLMIATCALGSLMTSEWIRVNFY
WLQALQIMAQPMVILSILMGVTTGLPPTEGPFASAMFNSLKTFSAAVATALIEGLGTGRERFHSNMLVDQLGNNAVVAGR
GVDSVHTLAQLAHRVHEQALVLTSADLYRVMACVAIALLFLVPILPVRIYPPWSTTPPSSH
>Mature_540_residues
TTANPSTTATDVPPVEQQTRPVSVQEEGIPSHPKPAGAALSLRLATGLIGVLLASLLAILNEQVTALALADVQGVFSIGR
DDGTWLTTLFEAANVATMVFAPWFGITFTLKRFTIGAVLATMLLGVLCPFAPSLQALYLLRVLQGVAGGCLPPMLIIVAL
RYLPPKIKLYGLAGYALTATFGPAVGTPLAALWTEYVSWEMVFWQIVPLGIASCVAVRWGLPDDPYKLERLSAFNWTGLL
TGFPAVAMLVTGLLQGDRLDWLSSEFIRVMFGGGVVLLTVFLFNEWFHPLPFFKLQLLARRNFAHGLMTLAGAVILLVGV
AAIPGQYLARVHAYRPLQTAPLSLLLALPLLITLPLTAAVLNLRRVDSRWVMTAGLLLMIATCALGSLMTSEWIRVNFYW
LQALQIMAQPMVILSILMGVTTGLPPTEGPFASAMFNSLKTFSAAVATALIEGLGTGRERFHSNMLVDQLGNNAVVAGRG
VDSVHTLAQLAHRVHEQALVLTSADLYRVMACVAIALLFLVPILPVRIYPPWSTTPPSSH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=494, Percent_Identity=25.7085020242915, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=23.943661971831, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58295; Mature: 58164

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure 
TTANPSTTATDVPPVEQQTRPVSVQEEGIPSHPKPAGAALSLRLATGLIGVLLASLLAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]