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The map label for this gene is mdlA [H]

Identifier: 73538269

GI number: 73538269

Start: 1101529

End: 1102629

Strand: Direct

Name: mdlA [H]

Synonym: Reut_B4441

Alternate gene names: 73538269

Gene position: 1101529-1102629 (Clockwise)

Preceding gene: 73538268

Following gene: 73538270

Centisome position: 40.41

GC content: 68.21

Gene sequence:

>1101_bases
GTGACACGCTCGCATGCCACGCCGATCCGGTCCATCGAAGCCAGGGCTTATCGGATTCCCTTGCCGGCTCCGGAATCCGA
CGGGACGCTGGAGTGGGACCATGTCACGCTGGTGGTGGTGCATGCCGCCGCCGGCGGCGTCACCGGGTGCGGGTATGGGT
ACTCCGACCAGGCCGTCATCCCGATCATTGCGCAACTGCTGGTGCCGCGTTTGCTCGGCACCGACGCCATGGCCACGAAC
GCCAGCTGGCGCGCCATGAAGGGCGCCGTGCGCAACCTCGGCGTATCGGGGCTGTGCGCCCAGGCGATTTCGGCCGTCGA
CATTGCACTGTGGGACCTGAAGGCGCGCCTGCTCGACGTGTCGCTCATGCAGTTGCTCGGCGCCGCGCGCACCTCGGTGC
CCGGCTATGCCAGCGGCGGGTTCACCAGCCTGTCCCATGAAGCGCTGCACGACGCTGCGCGCGCCTGGCGCGACGCGGGG
TTCTCGCGCGTGAAGATAAAGGTCGGGCGCCACCCCGACGACGACCCGGCCCGCGTCGCCACGGTGCGCGATGCCGTTGG
CGCGGAGGTCGAAGTGTTTGTCGATGCGAATGGCGCGTACACGGTCGCGCAGGCGCTGCAGATGGCGCAGGCCTTCGCAC
CGTTCCGCATCACATGGTTCGAGGAGCCTGTTTCGTCGGACGACATTACGGGCCTGCGCGCGGTGCGCGAGCACACGCCA
CCGGGGCTCGCCGTCGCGGCGGGCGAGTATGGCTTTACGGGCGACGATTTCCGGCGACTGCTGGAGCCGCGTTGCGTGGA
TGTACTGCAGGCGGATGCAACACGCTGCGGCATCACCGGATGGCTGCGCGCCGTGAACCTCTGCGAAGCCTTCCATGTGC
CGCTGTCCAGCCACTGTGCCCCTGCGCTGCATGCGAGCCTGTGCTGCGCGGCCTCTCCGGCCGTCCACGTCGAAGTGTTC
GAGGATCATGCCCACATTGAAGCGCAGTACTTCGACGGTATACCCACGCTGCACGACGGCAACCTGTGCCCGCACGCGAA
CCAGCCCGGGCTCGGCCTGCGCTTCCGCGAAGGGGATGCACGATGTCACCTGGCCTGCTAG

Upstream 100 bases:

>100_bases
GCGATACCGATGCCGGTGGCACGCTGCGCGCGACGTTCCGGCAGGTCTGGGAATCGCTGACGGCTTCGTCGAAGCGGCAT
GCGGACAAGGATGCGACGCC

Downstream 100 bases:

>100_bases
CCTCGGCCCACGGCGAAGGCTTCGCCGCGCTGGAGACGGCGCTCCGCGATACCGTCGATGCGGAAGTCCGCTTTGACGCG
GGAAGCCGCGCGCTGTATGC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTRSHATPIRSIEARAYRIPLPAPESDGTLEWDHVTLVVVHAAAGGVTGCGYGYSDQAVIPIIAQLLVPRLLGTDAMATN
ASWRAMKGAVRNLGVSGLCAQAISAVDIALWDLKARLLDVSLMQLLGAARTSVPGYASGGFTSLSHEALHDAARAWRDAG
FSRVKIKVGRHPDDDPARVATVRDAVGAEVEVFVDANGAYTVAQALQMAQAFAPFRITWFEEPVSSDDITGLRAVREHTP
PGLAVAAGEYGFTGDDFRRLLEPRCVDVLQADATRCGITGWLRAVNLCEAFHVPLSSHCAPALHASLCCAASPAVHVEVF
EDHAHIEAQYFDGIPTLHDGNLCPHANQPGLGLRFREGDARCHLAC

Sequences:

>Translated_366_residues
MTRSHATPIRSIEARAYRIPLPAPESDGTLEWDHVTLVVVHAAAGGVTGCGYGYSDQAVIPIIAQLLVPRLLGTDAMATN
ASWRAMKGAVRNLGVSGLCAQAISAVDIALWDLKARLLDVSLMQLLGAARTSVPGYASGGFTSLSHEALHDAARAWRDAG
FSRVKIKVGRHPDDDPARVATVRDAVGAEVEVFVDANGAYTVAQALQMAQAFAPFRITWFEEPVSSDDITGLRAVREHTP
PGLAVAAGEYGFTGDDFRRLLEPRCVDVLQADATRCGITGWLRAVNLCEAFHVPLSSHCAPALHASLCCAASPAVHVEVF
EDHAHIEAQYFDGIPTLHDGNLCPHANQPGLGLRFREGDARCHLAC
>Mature_365_residues
TRSHATPIRSIEARAYRIPLPAPESDGTLEWDHVTLVVVHAAAGGVTGCGYGYSDQAVIPIIAQLLVPRLLGTDAMATNA
SWRAMKGAVRNLGVSGLCAQAISAVDIALWDLKARLLDVSLMQLLGAARTSVPGYASGGFTSLSHEALHDAARAWRDAGF
SRVKIKVGRHPDDDPARVATVRDAVGAEVEVFVDANGAYTVAQALQMAQAFAPFRITWFEEPVSSDDITGLRAVREHTPP
GLAVAAGEYGFTGDDFRRLLEPRCVDVLQADATRCGITGWLRAVNLCEAFHVPLSSHCAPALHASLCCAASPAVHVEVFE
DHAHIEAQYFDGIPTLHDGNLCPHANQPGLGLRFREGDARCHLAC

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI186972148, Length=241, Percent_Identity=27.3858921161826, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI42544119, Length=241, Percent_Identity=27.3858921161826, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI42544117, Length=240, Percent_Identity=25.4166666666667, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1787864, Length=373, Percent_Identity=26.5415549597855, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994953, Length=311, Percent_Identity=27.0096463022508, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI226510960, Length=192, Percent_Identity=30.7291666666667, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 39081; Mature: 38949

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSHATPIRSIEARAYRIPLPAPESDGTLEWDHVTLVVVHAAAGGVTGCGYGYSDQAVI
CCCCCCCCHHHHCCCEEECCCCCCCCCCCEEECCEEEEEEEECCCCCCCCCCCCCCCCHH
PIIAQLLVPRLLGTDAMATNASWRAMKGAVRNLGVSGLCAQAISAVDIALWDLKARLLDV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLMQLLGAARTSVPGYASGGFTSLSHEALHDAARAWRDAGFSRVKIKVGRHPDDDPARVA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
TVRDAVGAEVEVFVDANGAYTVAQALQMAQAFAPFRITWFEEPVSSDDITGLRAVREHTP
HHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCC
PGLAVAAGEYGFTGDDFRRLLEPRCVDVLQADATRCGITGWLRAVNLCEAFHVPLSSHCA
CCEEEECCCCCCCHHHHHHHHCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCHHHCCH
PALHASLCCAASPAVHVEVFEDHAHIEAQYFDGIPTLHDGNLCPHANQPGLGLRFREGDA
HHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCC
RCHLAC
CEEECC
>Mature Secondary Structure 
TRSHATPIRSIEARAYRIPLPAPESDGTLEWDHVTLVVVHAAAGGVTGCGYGYSDQAVI
CCCCCCCHHHHCCCEEECCCCCCCCCCCEEECCEEEEEEEECCCCCCCCCCCCCCCCHH
PIIAQLLVPRLLGTDAMATNASWRAMKGAVRNLGVSGLCAQAISAVDIALWDLKARLLDV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLMQLLGAARTSVPGYASGGFTSLSHEALHDAARAWRDAGFSRVKIKVGRHPDDDPARVA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHH
TVRDAVGAEVEVFVDANGAYTVAQALQMAQAFAPFRITWFEEPVSSDDITGLRAVREHTP
HHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCC
PGLAVAAGEYGFTGDDFRRLLEPRCVDVLQADATRCGITGWLRAVNLCEAFHVPLSSHCA
CCEEEECCCCCCCHHHHHHHHCHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCHHHCCH
PALHASLCCAASPAVHVEVFEDHAHIEAQYFDGIPTLHDGNLCPHANQPGLGLRFREGDA
HHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCC
RCHLAC
CEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]