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The map label for this gene is ydaP [H]

Identifier: 73538268

GI number: 73538268

Start: 1099706

End: 1101532

Strand: Direct

Name: ydaP [H]

Synonym: Reut_B4440

Alternate gene names: 73538268

Gene position: 1099706-1101532 (Clockwise)

Preceding gene: 73538266

Following gene: 73538269

Centisome position: 40.34

GC content: 67.0

Gene sequence:

>1827_bases
ATGGAGACCGCCATGGCAATGACTGTTTCGGATTTTGTGCTCAAGCGCTTGCGGGAATGGGGCGTCCGGCGTGTATTTGG
CTACCCGGGCGATGGCATCAACGGGCTGATGGGAGCGTTCGGTCGCGCCGACAACGACCCGGAGTTCATCCAGGCGCGCC
ATGAGGAACTGGCCGCCTTCATGGCGTGCGCGCATGCCAAGTTCACCGGCGAGGCCGGGGTATGCCTGGCCACGTCCGGC
CCCGGCGCCATTCACCTGCTCAATGGCCTGTACGACGCAAAAATGGACCACCAGCCCGTGGTGGCCATCGTCGGCCAGCA
AAAGCGCGCGGCGCTGGGCGGCGACTACCAGCAGGAGGTCGACCTGACCACGCTGATGAAGGACGTGGCGCGCGACTTCG
TGGCGATGGCGATCGATCCCGCGCAGATGCGTCACCTGGTGGACCGCGCCATGCGCATCGCCATCGAACGGCGCACGGTC
ACGTGCATCATCATTCCGAACGACGTGCAGAACCTGCCTGCCGTACCCGTGCCGCCACGCGAGCACGGCACGGTGCATAC
CGGCATCGGCTACACCGCGCGCGCGCTGGTGCCGCCGGAGCCGGATCTTCGCCGCGCCGCCGACGTGCTCAATGCGGGCA
GCAAGGTGGCGATGCTGGTGGGCGCCGGGGCCCTGCAAGCCACCGATGAAGTCATCGAGGTGGCGCGCCGGCTCGGCGCA
GGGGTGGCCAAGGCGCTGCTGGGCAAGGCCGCCGTGCCGGACGACCTGCCCTTTGTCACCGGCAGCATCGGCCTGCTCGG
CACCAAGCCAAGCTGGGAGCTGATGACGGGATGCGACACCTTGCTGATGGTGGGCTCGGCCTTCCCGTATTCCGAGTTCC
TGCCCGAAGAAGGCAAGGCCCGCGGGGTGCAGATCGACATCGACGCACGCATGCTGAACCTGCGCTACCCGATGGAGATC
TGCCTGGAGGGCGACAGCGCGGCCACCCTGCGTGCGCTGCTGCCGATGCTGGAGCAGAAGCCGCTCGCCTGGCAGCAGGA
GATCGAACATAACGTCGCCGAGTGGTGGCAGGTGCTGGCCCAGCGCGCTGCCGCCAAGGCCAACCCGATCAATCCACAGC
GCGTGTTCGTGGAACTGTCCGAACGGCTTCCCGACGGCTGCATCCTGACCTGCGACTCTGGCTCCGCCGCCAACTGGTAT
GCGCGCGACCTGAAGATACGTCGCGGCATGATGGCATCGCTGAGCGGCGGGCTCGCGACGATGGGGCCTGCCGTGCCGTA
TGCGATTGCGGCAAGGTACGCGCACCCCGATCGTCCCGTGATTGCGATGGTTGGGGATGGCGCCATGCAGATGAACGGCA
TCAACGCGCTGATCACGATCTCGCGCGACTGGAAGCGCTGGTCGGGCGGATCACTGACCATCATCGTGCTGAACAACGGC
GACCTGAACCAGGTCACCTGGGAGCAGCGCGTCATGGAAGGCGACCCCAAGTTTGCCGTGTCGCAGGACCTGCCCGTGTT
CGGGTATGCGGCGTTCGCGCAGTTGCTCGGCCTGCAGGGCGTGCGCGTCGACCGCCCCGAGGGGATTGGCCCGGCCCTGG
ACCAGGCACTGGCCGCCAGGATCCCCACCGTGCTCGAAATGGTCACGGACCCCGATGTTCCGCCGCTGCCGCCGCATATT
TCGGCGAAGCAGGCACGCGACTACATCGCGGCCATGCTCCATGGCGATACCGATGCCGGTGGCACGCTGCGCGCGACGTT
CCGGCAGGTCTGGGAATCGCTGACGGCTTCGTCGAAGCGGCATGCGGACAAGGATGCGACGCCGTGA

Upstream 100 bases:

>100_bases
GCTGTGCGCTGCGCTCACGATTGCGGCCGTTGCGTATCGCCGGGTGCGTACCGGCGCCCGTGGTCATCGGCATGCGTGAT
GCATGGCGGTGTCCAATAGG

Downstream 100 bases:

>100_bases
CACGCTCGCATGCCACGCCGATCCGGTCCATCGAAGCCAGGGCTTATCGGATTCCCTTGCCGGCTCCGGAATCCGACGGG
ACGCTGGAGTGGGACCATGT

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
METAMAMTVSDFVLKRLREWGVRRVFGYPGDGINGLMGAFGRADNDPEFIQARHEELAAFMACAHAKFTGEAGVCLATSG
PGAIHLLNGLYDAKMDHQPVVAIVGQQKRAALGGDYQQEVDLTTLMKDVARDFVAMAIDPAQMRHLVDRAMRIAIERRTV
TCIIIPNDVQNLPAVPVPPREHGTVHTGIGYTARALVPPEPDLRRAADVLNAGSKVAMLVGAGALQATDEVIEVARRLGA
GVAKALLGKAAVPDDLPFVTGSIGLLGTKPSWELMTGCDTLLMVGSAFPYSEFLPEEGKARGVQIDIDARMLNLRYPMEI
CLEGDSAATLRALLPMLEQKPLAWQQEIEHNVAEWWQVLAQRAAAKANPINPQRVFVELSERLPDGCILTCDSGSAANWY
ARDLKIRRGMMASLSGGLATMGPAVPYAIAARYAHPDRPVIAMVGDGAMQMNGINALITISRDWKRWSGGSLTIIVLNNG
DLNQVTWEQRVMEGDPKFAVSQDLPVFGYAAFAQLLGLQGVRVDRPEGIGPALDQALAARIPTVLEMVTDPDVPPLPPHI
SAKQARDYIAAMLHGDTDAGGTLRATFRQVWESLTASSKRHADKDATP

Sequences:

>Translated_608_residues
METAMAMTVSDFVLKRLREWGVRRVFGYPGDGINGLMGAFGRADNDPEFIQARHEELAAFMACAHAKFTGEAGVCLATSG
PGAIHLLNGLYDAKMDHQPVVAIVGQQKRAALGGDYQQEVDLTTLMKDVARDFVAMAIDPAQMRHLVDRAMRIAIERRTV
TCIIIPNDVQNLPAVPVPPREHGTVHTGIGYTARALVPPEPDLRRAADVLNAGSKVAMLVGAGALQATDEVIEVARRLGA
GVAKALLGKAAVPDDLPFVTGSIGLLGTKPSWELMTGCDTLLMVGSAFPYSEFLPEEGKARGVQIDIDARMLNLRYPMEI
CLEGDSAATLRALLPMLEQKPLAWQQEIEHNVAEWWQVLAQRAAAKANPINPQRVFVELSERLPDGCILTCDSGSAANWY
ARDLKIRRGMMASLSGGLATMGPAVPYAIAARYAHPDRPVIAMVGDGAMQMNGINALITISRDWKRWSGGSLTIIVLNNG
DLNQVTWEQRVMEGDPKFAVSQDLPVFGYAAFAQLLGLQGVRVDRPEGIGPALDQALAARIPTVLEMVTDPDVPPLPPHI
SAKQARDYIAAMLHGDTDAGGTLRATFRQVWESLTASSKRHADKDATP
>Mature_608_residues
METAMAMTVSDFVLKRLREWGVRRVFGYPGDGINGLMGAFGRADNDPEFIQARHEELAAFMACAHAKFTGEAGVCLATSG
PGAIHLLNGLYDAKMDHQPVVAIVGQQKRAALGGDYQQEVDLTTLMKDVARDFVAMAIDPAQMRHLVDRAMRIAIERRTV
TCIIIPNDVQNLPAVPVPPREHGTVHTGIGYTARALVPPEPDLRRAADVLNAGSKVAMLVGAGALQATDEVIEVARRLGA
GVAKALLGKAAVPDDLPFVTGSIGLLGTKPSWELMTGCDTLLMVGSAFPYSEFLPEEGKARGVQIDIDARMLNLRYPMEI
CLEGDSAATLRALLPMLEQKPLAWQQEIEHNVAEWWQVLAQRAAAKANPINPQRVFVELSERLPDGCILTCDSGSAANWY
ARDLKIRRGMMASLSGGLATMGPAVPYAIAARYAHPDRPVIAMVGDGAMQMNGINALITISRDWKRWSGGSLTIIVLNNG
DLNQVTWEQRVMEGDPKFAVSQDLPVFGYAAFAQLLGLQGVRVDRPEGIGPALDQALAARIPTVLEMVTDPDVPPLPPHI
SAKQARDYIAAMLHGDTDAGGTLRATFRQVWESLTASSKRHADKDATP

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=591, Percent_Identity=24.3654822335025, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1787096, Length=576, Percent_Identity=31.7708333333333, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI1790104, Length=512, Percent_Identity=27.734375, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI87081685, Length=557, Percent_Identity=28.3662477558348, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1786717, Length=575, Percent_Identity=25.5652173913043, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788716, Length=575, Percent_Identity=22.6086956521739, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=26.9503546099291, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320816, Length=579, Percent_Identity=20.8981001727116, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922626, Length=598, Percent_Identity=23.9130434782609, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65650; Mature: 65650

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METAMAMTVSDFVLKRLREWGVRRVFGYPGDGINGLMGAFGRADNDPEFIQARHEELAAF
CCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
MACAHAKFTGEAGVCLATSGPGAIHLLNGLYDAKMDHQPVVAIVGQQKRAALGGDYQQEV
HHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCHHHH
DLTTLMKDVARDFVAMAIDPAQMRHLVDRAMRIAIERRTVTCIIIPNDVQNLPAVPVPPR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECEEEEEEECCCCCCCCCCCCCCC
EHGTVHTGIGYTARALVPPEPDLRRAADVLNAGSKVAMLVGAGALQATDEVIEVARRLGA
CCCCEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCH
GVAKALLGKAAVPDDLPFVTGSIGLLGTKPSWELMTGCDTLLMVGSAFPYSEFLPEEGKA
HHHHHHHCCCCCCCCCCEEECCCEEECCCCCCHHHHCCHHHHHHCCCCCHHHHCCCCCCC
RGVQIDIDARMLNLRYPMEICLEGDSAATLRALLPMLEQKPLAWQQEIEHNVAEWWQVLA
CCEEEEECEEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QRAAAKANPINPQRVFVELSERLPDGCILTCDSGSAANWYARDLKIRRGMMASLSGGLAT
HHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHH
MGPAVPYAIAARYAHPDRPVIAMVGDGAMQMNGINALITISRDWKRWSGGSLTIIVLNNG
CCCCHHHHHHHHCCCCCCCEEEEECCCCEEECCCEEEEEEECCCCCCCCCCEEEEEECCC
DLNQVTWEQRVMEGDPKFAVSQDLPVFGYAAFAQLLGLQGVRVDRPEGIGPALDQALAAR
CCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
IPTVLEMVTDPDVPPLPPHISAKQARDYIAAMLHGDTDAGGTLRATFRQVWESLTASSKR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
HADKDATP
CCCCCCCC
>Mature Secondary Structure
METAMAMTVSDFVLKRLREWGVRRVFGYPGDGINGLMGAFGRADNDPEFIQARHEELAAF
CCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
MACAHAKFTGEAGVCLATSGPGAIHLLNGLYDAKMDHQPVVAIVGQQKRAALGGDYQQEV
HHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCHHHH
DLTTLMKDVARDFVAMAIDPAQMRHLVDRAMRIAIERRTVTCIIIPNDVQNLPAVPVPPR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECEEEEEEECCCCCCCCCCCCCCC
EHGTVHTGIGYTARALVPPEPDLRRAADVLNAGSKVAMLVGAGALQATDEVIEVARRLGA
CCCCEEECCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCH
GVAKALLGKAAVPDDLPFVTGSIGLLGTKPSWELMTGCDTLLMVGSAFPYSEFLPEEGKA
HHHHHHHCCCCCCCCCCEEECCCEEECCCCCCHHHHCCHHHHHHCCCCCHHHHCCCCCCC
RGVQIDIDARMLNLRYPMEICLEGDSAATLRALLPMLEQKPLAWQQEIEHNVAEWWQVLA
CCEEEEECEEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QRAAAKANPINPQRVFVELSERLPDGCILTCDSGSAANWYARDLKIRRGMMASLSGGLAT
HHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHH
MGPAVPYAIAARYAHPDRPVIAMVGDGAMQMNGINALITISRDWKRWSGGSLTIIVLNNG
CCCCHHHHHHHHCCCCCCCEEEEECCCCEEECCCEEEEEEECCCCCCCCCCEEEEEECCC
DLNQVTWEQRVMEGDPKFAVSQDLPVFGYAAFAQLLGLQGVRVDRPEGIGPALDQALAAR
CCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
IPTVLEMVTDPDVPPLPPHISAKQARDYIAAMLHGDTDAGGTLRATFRQVWESLTASSKR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
HADKDATP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]