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The map label for this gene is benM [H]

Identifier: 73538230

GI number: 73538230

Start: 1058521

End: 1059438

Strand: Reverse

Name: benM [H]

Synonym: Reut_B4401

Alternate gene names: 73538230

Gene position: 1059438-1058521 (Counterclockwise)

Preceding gene: 73538236

Following gene: 161611272

Centisome position: 38.86

GC content: 64.71

Gene sequence:

>918_bases
TTGAGACATCTGCGCTACTTCGTCGCCGTCGCGGAGGAACGCAACTTCACCCGCGCGGCCGAGCGCCTGCATATTGCGCA
ACCTCCGCTGAGCCGCCAGATCCAGCAATTAGAGGAGGTGCTCGGTGTCCAGCTGTTCGAGCGCAATACGCGGCCGCTGC
GGCTGACCGACACCGGGCGCTTCTTTTACACGCACGCGCTGCAGCTGCTAGCGCAGACGTCGGAGCTGGAGGCCATGACG
CGGCGCGTGGCCAAGATAGAGCGCAAGATGTCGGTCGGCTTTGTCGGCTCCACGCTGTACGGCATGCTGCCGCGGATCAT
CCGGCGCTTTCGCACCGAGCATCCCGATATCGAGATGAGCCTGCACGAGATGTCGACCATGGACCAGATCAAGGCGCTCA
AGGACGGGCGCATCGATGTGGGCTTCGGCCGCATCCGGCACGACGACCCGGCCGTGCGGCGCGTGGTGCTGCGCGAGGAG
CGCATGATCGTGGCGTTGCCGCTCGGGCACCCGCTGGCTGCGGGGCGAAAGACGCTGTCGCTGCATGACCTGGTGGAGGA
GTCGCTGATCATCTTCCCCAAGGCGCCGCGGCCCAGTTTCGCCGACCAGGTGCTTGCGGCGTTCCATGATCGCGCCTTGA
AGCCGCGCCGGCTGCATGAGGTGCGCGAACTGCAGATCGCGCTGGGGCTGGTCGCGATGGGCGAGGGGATTTCGGTGGTG
CCGAGCAGTGTGTACGGCCTCAAGCGCGACGATGTGAGCTATGTCGAACTCGACGACCCGAGCCTGGTGTCGCCGATCAT
CATGAGCATGCGCATGCTCGATGAATCGCAGGACCTGCGTGAGATGCAGGCGATGATCTACCAGCTCTACGAAGAACTGG
GCATTCCGTACAACCCGCCGGGCCACGAGAGCGCTTAA

Upstream 100 bases:

>100_bases
ACTTGCCGTTCGACTTGGTACGGAGCATAGTTGACGGGCATTTGAGGCGTCCAACACTGATTTAGTATCTTCTTTATACC
TGCTGGGTATGACCATAGAA

Downstream 100 bases:

>100_bases
GCCATACCTAACGGGTATGCGACGAGACATCATCGGTCTTGGACAGCCGGAAATCAGGCTCCCATACTTTCGCATACGAC
ATCCACTGTATCGAGAAGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRHLRYFVAVAEERNFTRAAERLHIAQPPLSRQIQQLEEVLGVQLFERNTRPLRLTDTGRFFYTHALQLLAQTSELEAMT
RRVAKIERKMSVGFVGSTLYGMLPRIIRRFRTEHPDIEMSLHEMSTMDQIKALKDGRIDVGFGRIRHDDPAVRRVVLREE
RMIVALPLGHPLAAGRKTLSLHDLVEESLIIFPKAPRPSFADQVLAAFHDRALKPRRLHEVRELQIALGLVAMGEGISVV
PSSVYGLKRDDVSYVELDDPSLVSPIIMSMRMLDESQDLREMQAMIYQLYEELGIPYNPPGHESA

Sequences:

>Translated_305_residues
MRHLRYFVAVAEERNFTRAAERLHIAQPPLSRQIQQLEEVLGVQLFERNTRPLRLTDTGRFFYTHALQLLAQTSELEAMT
RRVAKIERKMSVGFVGSTLYGMLPRIIRRFRTEHPDIEMSLHEMSTMDQIKALKDGRIDVGFGRIRHDDPAVRRVVLREE
RMIVALPLGHPLAAGRKTLSLHDLVEESLIIFPKAPRPSFADQVLAAFHDRALKPRRLHEVRELQIALGLVAMGEGISVV
PSSVYGLKRDDVSYVELDDPSLVSPIIMSMRMLDESQDLREMQAMIYQLYEELGIPYNPPGHESA
>Mature_305_residues
MRHLRYFVAVAEERNFTRAAERLHIAQPPLSRQIQQLEEVLGVQLFERNTRPLRLTDTGRFFYTHALQLLAQTSELEAMT
RRVAKIERKMSVGFVGSTLYGMLPRIIRRFRTEHPDIEMSLHEMSTMDQIKALKDGRIDVGFGRIRHDDPAVRRVVLREE
RMIVALPLGHPLAAGRKTLSLHDLVEESLIIFPKAPRPSFADQVLAAFHDRALKPRRLHEVRELQIALGLVAMGEGISVV
PSSVYGLKRDDVSYVELDDPSLVSPIIMSMRMLDESQDLREMQAMIYQLYEELGIPYNPPGHESA

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=31.3793103448276, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1787879, Length=270, Percent_Identity=32.5925925925926, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=30.5660377358491, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1790399, Length=263, Percent_Identity=29.6577946768061, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=29.5620437956204, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=264, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081904, Length=203, Percent_Identity=31.0344827586207, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787601, Length=245, Percent_Identity=27.3469387755102, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=28.3400809716599, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788481, Length=181, Percent_Identity=32.0441988950276, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=29.718875502008, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=144, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788748, Length=143, Percent_Identity=28.6713286713287, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790783, Length=143, Percent_Identity=29.3706293706294, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789204, Length=248, Percent_Identity=26.6129032258064, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082132, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=23.5059760956175, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693193, Length=172, Percent_Identity=34.3023255813954, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=195, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790262, Length=193, Percent_Identity=26.9430051813472, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786401, Length=173, Percent_Identity=31.2138728323699, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34925; Mature: 34925

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLRYFVAVAEERNFTRAAERLHIAQPPLSRQIQQLEEVLGVQLFERNTRPLRLTDTGR
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCH
FFYTHALQLLAQTSELEAMTRRVAKIERKMSVGFVGSTLYGMLPRIIRRFRTEHPDIEMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
LHEMSTMDQIKALKDGRIDVGFGRIRHDDPAVRRVVLREERMIVALPLGHPLAAGRKTLS
HHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHCCCHHHH
LHDLVEESLIIFPKAPRPSFADQVLAAFHDRALKPRRLHEVRELQIALGLVAMGEGISVV
HHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEC
PSSVYGLKRDDVSYVELDDPSLVSPIIMSMRMLDESQDLREMQAMIYQLYEELGIPYNPP
CHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
GHESA
CCCCC
>Mature Secondary Structure
MRHLRYFVAVAEERNFTRAAERLHIAQPPLSRQIQQLEEVLGVQLFERNTRPLRLTDTGR
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCH
FFYTHALQLLAQTSELEAMTRRVAKIERKMSVGFVGSTLYGMLPRIIRRFRTEHPDIEMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
LHEMSTMDQIKALKDGRIDVGFGRIRHDDPAVRRVVLREERMIVALPLGHPLAAGRKTLS
HHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHCCCHHHH
LHDLVEESLIIFPKAPRPSFADQVLAAFHDRALKPRRLHEVRELQIALGLVAMGEGISVV
HHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEC
PSSVYGLKRDDVSYVELDDPSLVSPIIMSMRMLDESQDLREMQAMIYQLYEELGIPYNPP
CHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
GHESA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]