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The map label for this gene is catB [H]

Identifier: 161611272

GI number: 161611272

Start: 1057302

End: 1058420

Strand: Reverse

Name: catB [H]

Synonym: Reut_B4400

Alternate gene names: 161611272

Gene position: 1058420-1057302 (Counterclockwise)

Preceding gene: 73538230

Following gene: 73538225

Centisome position: 38.82

GC content: 65.42

Gene sequence:

>1119_bases
ATGAAAGCGACGATTTCTTCCATTGAGGCCATCCTGGTCGACCTGCCCACCATCCGGGCCCATCAACTGGCCATGGCCAC
CATGCAGCAGCAGACGCTGGTGATCGTGCGGCTGCGCTGCAGCGACGGCATCGAAGGCATCGGCGAGGCCACCACCATTG
GTGGCCTTTCCTATGGCGATGAAAGCCCCGAAGGCATCAAGCTGGCGATCGACACTTACCTGGCGCCTGCGCTGGCCGGC
GAAGATGCGACCAATGTCCATGCCGCCATGGCACGCCTGAACAAGATCGCGCGCGGCAATCGCTTTGCCAAGTCGGCTCT
GGAGACAGCGCTGCTTGATGCGCAAGGCAAACGCCTCGGCGTGCCGCTCGCCACGCTGCTCGGCGGCGCCGTGCGCGCCA
CGCTCCCTGTCCTGTGGACGCTGGCCAGCGGTGATACGGCACGCGATATCGACGAAGCCGAGCGCCTGCTGGCCGAACGT
CGTCACAACACGTTCAAGCTCAAGATCGGCCGGCGCAGCGTGCGTGATGACGTGGCGCATGTGTCCGCCATCAAGCGCGC
GCTGGGTGACCGTGCACGCGTGACGGTGGATATCAACCAGGCGTGGAACGAGGCCGATGCCGCGGGTGGTATTGCCATGC
TCGAAGCCGCTGGCATCGACCTGATCGAGCAACCCACGCCGCGTGAGCAACGTACGGCGCTGGCCCGTCTGGCGGCGCGC
TTCGTCGTGCCGATCATGGCGGATGAAGCGGTATGTGGCCCAGAAGATGCCATGGAACTCGCGCGCATCGGCGGTGCCGA
TGTGTTCGCGCTGAAGATCGCGAAATCGGGCGGCATCTTCGGCATGCTGCGCACGGCAGCGGTTGGCGATGCCGCGGGTA
TCGCGCTATACGGCGGTACGATGCTGGAAGGCAGCGTGGGCACCATCGCTGCCGCGCACGGCTTCTGCACGCTGCCGCAA
CTTGCGTGGGGCACAGAGTTGTTCGGCCCGCTGCTGCTCAAGGACGATGTAGTCGTGCAGCGGCCCGAATATCGCGATTT
CAGCCTGCACCTGCCCGCAGGCCCGGGACTGGGCCTCGCGCTCGATGAAGACAAGCTCGCGCACTACCGTCGCGCGTGA

Upstream 100 bases:

>100_bases
GCCATACCTAACGGGTATGCGACGAGACATCATCGGTCTTGGACAGCCGGAAATCAGGCTCCCATACTTTCGCATACGAC
ATCCACTGTATCGAGAAGGT

Downstream 100 bases:

>100_bases
GCCAGCGGGAGTGTCCGTTCAGGGCACTACCGACAGGAACAGGTAGGCGGCCAGGATCACAAGGTGCACGACACCTTGCA
GGATCGTGGTTCGGCCCCTG

Product: muconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLAIDTYLAPALAG
EDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRATLPVLWTLASGDTARDIDEAERLLAER
RHNTFKLKIGRRSVRDDVAHVSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLALDEDKLAHYRRA

Sequences:

>Translated_372_residues
MKATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLAIDTYLAPALAG
EDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRATLPVLWTLASGDTARDIDEAERLLAER
RHNTFKLKIGRRSVRDDVAHVSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLALDEDKLAHYRRA
>Mature_372_residues
MKATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLAIDTYLAPALAG
EDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRATLPVLWTLASGDTARDIDEAERLLAER
RHNTFKLKIGRRSVRDDVAHVSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLALDEDKLAHYRRA

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=322, Percent_Identity=24.223602484472, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081876, Length=295, Percent_Identity=27.1186440677966, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 39337; Mature: 39337

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEECCCCCCC
ESPEGIKLAIDTYLAPALAGEDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLG
CCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
VPLATLLGGAVRATLPVLWTLASGDTARDIDEAERLLAERRHNTFKLKIGRRSVRDDVAH
CCHHHHHCHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
VSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
HHHHHHHCCCCEEEEEEHHHCCCCHHHCCCEEEEHHCCCHHHCCCCCHHHHHHHHHHHHH
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGT
HHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCE
MLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLA
EECCCCCCEEHHCCCCCCCHHHHCHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCCCEE
LDEDKLAHYRRA
ECHHHHHHHCCC
>Mature Secondary Structure
MKATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEECCCCCCC
ESPEGIKLAIDTYLAPALAGEDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLG
CCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
VPLATLLGGAVRATLPVLWTLASGDTARDIDEAERLLAERRHNTFKLKIGRRSVRDDVAH
CCHHHHHCHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
VSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
HHHHHHHCCCCEEEEEEHHHCCCCHHHCCCEEEEHHCCCHHHCCCCCHHHHHHHHHHHHH
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGT
HHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCE
MLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLA
EECCCCCCEEHHCCCCCCCHHHHCHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCCCEE
LDEDKLAHYRRA
ECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]