Click here to switch to the map view.

The map label for this gene is cls [H]

Identifier: 73537890

GI number: 73537890

Start: 696713

End: 698269

Strand: Direct

Name: cls [H]

Synonym: Reut_B4057

Alternate gene names: 73537890

Gene position: 696713-698269 (Clockwise)

Preceding gene: 73537887

Following gene: 73537891

Centisome position: 25.56

GC content: 65.32

Gene sequence:

>1557_bases
ATGCCGCAGAAGGCGTTTGTCGCGACGCAGCAGCGGCCGGGCGCCGCGCCACGCATATCGCTGTGCCAAAATAAGAACCA
TCGTGATCCGGTTCCGCGTGGCGGACACCTGGGAAGCCTGCACATGCTAAGCCCGAGCGTTGTCGCGACGGTCATCCTGA
TTTTTCACGTCATTGGCGTGCTGGCCGCATTGCACGCCGTCATGACCGTGCGCACGGCGCCCGGGGCCATTGCCTGGGCG
GGATCGCTGGTGATGATGCCGTACCTCACACTGGTGCCTTACCTGATCTTCGGCAGGAGCCGTTTCGCCGGCTATGTCAA
TGCGCGCCGCTTCAATGACGACAGGCTGCGCGAGATCCGCCATGGCATGACCCCTGAAGACCGCGACGCCCGCAGCGCCT
GCGTGGTGCACGCACCGGAGCCAGCCTGCATGCGCGCGCTTCCGCGGCTGACCGGCATGCCTTGCCTGGCCAACAACGAT
GTGCGCCTGCTGGTCAACGGCACGACCACGTTCGAGGCCATCTTCGCAGCCGTGGCCGCAGCCAGGCAGGTGGTGATCGT
GCAGTTCTTCATCGTCCATGACGATGTACTCGGCCGGCGCCTGCAGGCATTGCTGTGCGAGCGCGCGCAGGCTGGCGTCA
AGGTCTATTTTCTCTACGACAGCATCGGTTGCCACGCGTTGCCGCGTGGCTATGTGAAGACCTTGATGGCGGCCGGCGTC
GAGGCGCGCGCGTTCTCGACGCACCGCGGCTTCGTCAACCGCTTCCAGCTCAACTTCCGCAACCATCGGAAGCTCGTGGT
CGTGGACGGGTCGCAGGCCTTCGTGGGGGGGCATAACGTCGGCGTTGAATATCTCGGCGAGCGGCCCCCGCTAGCGCCGT
GGCGCGACACCCATATCAGCGTGCGCGGCAGCGCTGTGCTGGATCTGCAGATGGCGTTTGCCGAGGATTGGTACTGGGCC
GCGCGCGAAGTGCCATACCTGCTGATGCCGCCTCCTGACGTGGGCGGGCGCATGACCTGCCAGGTCGTGCCGACGGGCCC
GGCCGACGCGCAGGAGACCTGCTCGCTGTTCTTCGTCGAAGCCATCCAGGCTGCGCGGCACCGGGTGTGGATCACGTCGC
CATACTTCGTGCCGGACGAAGCAGTCTTTGCCGTGCTGCGCCTGGCGGTACTGCGCGGGGTAGATGTGCGTATCCTGATT
CCCGCCAGGCCAGACCATCTCGTCGTGTTCGCCGCTTCCACGCTTTACGCGCATCAGGCCGTGGCCGCGGGGATCAAGCT
GTATCGCTACCAGCCGGGCTTTCTCCACCAGAAGGTCATCCTGGTCGACGACGACGCGGCGGCAGTCGGTACGGCGAACC
TCGACAACCGCTCGTTCCGGCTGAACTTCGAGTTGATGGTGATGACGGCGGACCGCGAGTTCGCCAGCCAGGTCGCTGAC
ATGCTGGAGGCCGATTTTGCGCAGGCGCGGCGCATCGGCGTAGACGAATTCCTGCGGGCGCCTGCGCCATTGCGTGTGGC
GATGCACGTGGCAAAGCTGTTTGCGCCGATCCTGTAA

Upstream 100 bases:

>100_bases
CGAGAGCGTATAAAGGCAAACGCGGCGGAACAATCGGACAAATCCGATTCCGGCCAGAAAATTGCGCTAGCTCAGATGTG
CGAACTGTTCGATCGAGGCA

Downstream 100 bases:

>100_bases
GCGAGGTGTCGGGAAGCAACGCCGCGCCCTATTGTGTACACTGTTCACTATTGGTTGGCAAGATAAGAACAGTATGTACA
GGGGGGAAATGATGAGCCGG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPQKAFVATQQRPGAAPRISLCQNKNHRDPVPRGGHLGSLHMLSPSVVATVILIFHVIGVLAALHAVMTVRTAPGAIAWA
GSLVMMPYLTLVPYLIFGRSRFAGYVNARRFNDDRLREIRHGMTPEDRDARSACVVHAPEPACMRALPRLTGMPCLANND
VRLLVNGTTTFEAIFAAVAAARQVVIVQFFIVHDDVLGRRLQALLCERAQAGVKVYFLYDSIGCHALPRGYVKTLMAAGV
EARAFSTHRGFVNRFQLNFRNHRKLVVVDGSQAFVGGHNVGVEYLGERPPLAPWRDTHISVRGSAVLDLQMAFAEDWYWA
AREVPYLLMPPPDVGGRMTCQVVPTGPADAQETCSLFFVEAIQAARHRVWITSPYFVPDEAVFAVLRLAVLRGVDVRILI
PARPDHLVVFAASTLYAHQAVAAGIKLYRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFELMVMTADREFASQVAD
MLEADFAQARRIGVDEFLRAPAPLRVAMHVAKLFAPIL

Sequences:

>Translated_518_residues
MPQKAFVATQQRPGAAPRISLCQNKNHRDPVPRGGHLGSLHMLSPSVVATVILIFHVIGVLAALHAVMTVRTAPGAIAWA
GSLVMMPYLTLVPYLIFGRSRFAGYVNARRFNDDRLREIRHGMTPEDRDARSACVVHAPEPACMRALPRLTGMPCLANND
VRLLVNGTTTFEAIFAAVAAARQVVIVQFFIVHDDVLGRRLQALLCERAQAGVKVYFLYDSIGCHALPRGYVKTLMAAGV
EARAFSTHRGFVNRFQLNFRNHRKLVVVDGSQAFVGGHNVGVEYLGERPPLAPWRDTHISVRGSAVLDLQMAFAEDWYWA
AREVPYLLMPPPDVGGRMTCQVVPTGPADAQETCSLFFVEAIQAARHRVWITSPYFVPDEAVFAVLRLAVLRGVDVRILI
PARPDHLVVFAASTLYAHQAVAAGIKLYRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFELMVMTADREFASQVAD
MLEADFAQARRIGVDEFLRAPAPLRVAMHVAKLFAPIL
>Mature_517_residues
PQKAFVATQQRPGAAPRISLCQNKNHRDPVPRGGHLGSLHMLSPSVVATVILIFHVIGVLAALHAVMTVRTAPGAIAWAG
SLVMMPYLTLVPYLIFGRSRFAGYVNARRFNDDRLREIRHGMTPEDRDARSACVVHAPEPACMRALPRLTGMPCLANNDV
RLLVNGTTTFEAIFAAVAAARQVVIVQFFIVHDDVLGRRLQALLCERAQAGVKVYFLYDSIGCHALPRGYVKTLMAAGVE
ARAFSTHRGFVNRFQLNFRNHRKLVVVDGSQAFVGGHNVGVEYLGERPPLAPWRDTHISVRGSAVLDLQMAFAEDWYWAA
REVPYLLMPPPDVGGRMTCQVVPTGPADAQETCSLFFVEAIQAARHRVWITSPYFVPDEAVFAVLRLAVLRGVDVRILIP
ARPDHLVVFAASTLYAHQAVAAGIKLYRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFELMVMTADREFASQVADM
LEADFAQARRIGVDEFLRAPAPLRVAMHVAKLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=489, Percent_Identity=26.9938650306748, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787007, Length=336, Percent_Identity=31.547619047619, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 57167; Mature: 57036

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQKAFVATQQRPGAAPRISLCQNKNHRDPVPRGGHLGSLHMLSPSVVATVILIFHVIGV
CCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
LAALHAVMTVRTAPGAIAWAGSLVMMPYLTLVPYLIFGRSRFAGYVNARRFNDDRLREIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCHHHHHHHH
HGMTPEDRDARSACVVHAPEPACMRALPRLTGMPCLANNDVRLLVNGTTTFEAIFAAVAA
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHHH
ARQVVIVQFFIVHDDVLGRRLQALLCERAQAGVKVYFLYDSIGCHALPRGYVKTLMAAGV
HHHEEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCC
EARAFSTHRGFVNRFQLNFRNHRKLVVVDGSQAFVGGHNVGVEYLGERPPLAPWRDTHIS
CHHEEHHHHCCCEEEEEEECCCCEEEEEECCEEEECCCCCCHHCCCCCCCCCCCCCCEEE
VRGSAVLDLQMAFAEDWYWAAREVPYLLMPPPDVGGRMTCQVVPTGPADAQETCSLFFVE
EECCEEEEEEEHHHHCHHHHHHCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
AIQAARHRVWITSPYFVPDEAVFAVLRLAVLRGVDVRILIPARPDHLVVFAASTLYAHQA
HHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHHHHHH
VAAGIKLYRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFELMVMTADREFASQVAD
HHHCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCEEEEEEEEEEEECCHHHHHHHHH
MLEADFAQARRIGVDEFLRAPAPLRVAMHVAKLFAPIL
HHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQKAFVATQQRPGAAPRISLCQNKNHRDPVPRGGHLGSLHMLSPSVVATVILIFHVIGV
CCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
LAALHAVMTVRTAPGAIAWAGSLVMMPYLTLVPYLIFGRSRFAGYVNARRFNDDRLREIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCHHHHHHHH
HGMTPEDRDARSACVVHAPEPACMRALPRLTGMPCLANNDVRLLVNGTTTFEAIFAAVAA
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHHH
ARQVVIVQFFIVHDDVLGRRLQALLCERAQAGVKVYFLYDSIGCHALPRGYVKTLMAAGV
HHHEEEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCC
EARAFSTHRGFVNRFQLNFRNHRKLVVVDGSQAFVGGHNVGVEYLGERPPLAPWRDTHIS
CHHEEHHHHCCCEEEEEEECCCCEEEEEECCEEEECCCCCCHHCCCCCCCCCCCCCCEEE
VRGSAVLDLQMAFAEDWYWAAREVPYLLMPPPDVGGRMTCQVVPTGPADAQETCSLFFVE
EECCEEEEEEEHHHHCHHHHHHCCCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
AIQAARHRVWITSPYFVPDEAVFAVLRLAVLRGVDVRILIPARPDHLVVFAASTLYAHQA
HHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHHHHHH
VAAGIKLYRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFELMVMTADREFASQVAD
HHHCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCEEEEEEEEEEEECCHHHHHHHHH
MLEADFAQARRIGVDEFLRAPAPLRVAMHVAKLFAPIL
HHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA