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The map label for this gene is eda [H]

Identifier: 73537857

GI number: 73537857

Start: 664709

End: 665350

Strand: Direct

Name: eda [H]

Synonym: Reut_B4024

Alternate gene names: 73537857

Gene position: 664709-665350 (Clockwise)

Preceding gene: 73537856

Following gene: 73537860

Centisome position: 24.38

GC content: 68.85

Gene sequence:

>642_bases
ATGCAACTTCAACCTTCCCCGCTGCTCGCGCGGCTGGCCAATGTGCCAGTCATTCCTGTGCTGGAATTCGGTTCCGTCGA
CGAAGCGCTGCATGTCAGCGAAGCGCTGGTGGCCGGCGGCCTGCCGCTGCTCGAAATCACGCTGCGCACGCCGGTCGCCA
TGGATGCCATGCGCGCCGTGGCCGCCAAGATTCCGGGCGCCTGCGTGGGCGCCGGTACGGTGCTGACCGTGGAACAGCTC
CACGCCGTGCGCGATGCCGGCGCGCAGTTCGCCGTATCTCCGGGCCTGACACCGACGCTGGCCGCTGGTGCGCAGGATGC
AGGCATCTCGCTGCTGCCGGGCGTGGCCACGGCCAGCGAGGCCATGGCCGCCCTGGAGTCCGGTTTCACCTTCCTGAAGT
TCTTCCCGGCCCAGGCCGCCGGCGGCGTGCCGATGCTCAAGTCGCTGTACGGGCCGCTGGCGCAACTGCGTTTCTGCCCC
ACCGGAGGTATTGATGCAGCGCTGGCACCGAGCTATCTCGCCTTGCCCAATGTGGTGTGCGTGGGCGGTTCATGGGTGGT
GCCGAAGGACGCCGTTGCTGCCGGCGACTGGGGCCGCATCCGCAAGCTCGCGGAAGACGCTGCGGCATTGCGCCGGGGCT
GA

Upstream 100 bases:

>100_bases
ATTCCTTCCGCGGAGACCGTGCTCGCCAGACTGGCGCAGTCGGTGTCCGTGATTGCCTGATCAGATCCACACTTCCTTGT
CCGCAACGAACCTGACTGCC

Downstream 100 bases:

>100_bases
GCCTCCGCATAGCCCGGAATGCAAAACGGCACGCCATGCGTGCCGTTTTGCCAGGGATGCGCTGTGCCCCGGATGCCGGG
GCGTACCGCTTACTTGTGGC

Product: 2-keto-3-deoxy-phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQLQPSPLLARLANVPVIPVLEFGSVDEALHVSEALVAGGLPLLEITLRTPVAMDAMRAVAAKIPGACVGAGTVLTVEQL
HAVRDAGAQFAVSPGLTPTLAAGAQDAGISLLPGVATASEAMAALESGFTFLKFFPAQAAGGVPMLKSLYGPLAQLRFCP
TGGIDAALAPSYLALPNVVCVGGSWVVPKDAVAAGDWGRIRKLAEDAAALRRG

Sequences:

>Translated_213_residues
MQLQPSPLLARLANVPVIPVLEFGSVDEALHVSEALVAGGLPLLEITLRTPVAMDAMRAVAAKIPGACVGAGTVLTVEQL
HAVRDAGAQFAVSPGLTPTLAAGAQDAGISLLPGVATASEAMAALESGFTFLKFFPAQAAGGVPMLKSLYGPLAQLRFCP
TGGIDAALAPSYLALPNVVCVGGSWVVPKDAVAAGDWGRIRKLAEDAAALRRG
>Mature_213_residues
MQLQPSPLLARLANVPVIPVLEFGSVDEALHVSEALVAGGLPLLEITLRTPVAMDAMRAVAAKIPGACVGAGTVLTVEQL
HAVRDAGAQFAVSPGLTPTLAAGAQDAGISLLPGVATASEAMAALESGFTFLKFFPAQAAGGVPMLKSLYGPLAQLRFCP
TGGIDAALAPSYLALPNVVCVGGSWVVPKDAVAAGDWGRIRKLAEDAAALRRG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=182, Percent_Identity=53.8461538461538, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI48994954, Length=129, Percent_Identity=37.984496124031, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21571; Mature: 21571

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQPSPLLARLANVPVIPVLEFGSVDEALHVSEALVAGGLPLLEITLRTPVAMDAMRAV
CCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
AAKIPGACVGAGTVLTVEQLHAVRDAGAQFAVSPGLTPTLAAGAQDAGISLLPGVATASE
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCEECCCCHHHHH
AMAALESGFTFLKFFPAQAAGGVPMLKSLYGPLAQLRFCPTGGIDAALAPSYLALPNVVC
HHHHHHCCCCEEEEECHHCCCCCHHHHHHHHHHHHEEECCCCCCCHHHCCCHHHCCCEEE
VGGSWVVPKDAVAAGDWGRIRKLAEDAAALRRG
ECCCEECCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLQPSPLLARLANVPVIPVLEFGSVDEALHVSEALVAGGLPLLEITLRTPVAMDAMRAV
CCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
AAKIPGACVGAGTVLTVEQLHAVRDAGAQFAVSPGLTPTLAAGAQDAGISLLPGVATASE
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCEECCCCHHHHH
AMAALESGFTFLKFFPAQAAGGVPMLKSLYGPLAQLRFCPTGGIDAALAPSYLALPNVVC
HHHHHHCCCCEEEEECHHCCCCCHHHHHHHHHHHHEEECCCCCCCHHHCCCHHHCCCEEE
VGGSWVVPKDAVAAGDWGRIRKLAEDAAALRRG
ECCCEECCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]