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The map label for this gene is rstB [H]

Identifier: 73537744

GI number: 73537744

Start: 542011

End: 543318

Strand: Direct

Name: rstB [H]

Synonym: Reut_B3910

Alternate gene names: 73537744

Gene position: 542011-543318 (Clockwise)

Preceding gene: 73537743

Following gene: 73537745

Centisome position: 19.88

GC content: 67.05

Gene sequence:

>1308_bases
GTGACCTTGACCTTGCCGCGCTGGGAGTGGCCCCGCACGCTCGGCGCGCGGCTGTTCCTGATCCTGCTCGCCGGCCTGGT
TGTCGCGCACGGCTTGTCGTTTGGCGTGCTGTTCGCCGAGCGTTACATCTCGGCGCGCCAGGTGATGCTGGGCACGCTGG
AAACCGATGTCGCCACCTCGGTGGCGATCCTTGATCGGTTACCGGCCAGCGAGCGGCCGCAGTGGCTGCCGCGCCTGAAT
CGTGGCAACTATCAGTACATCCTTGGGCCGGGCCTGCCCGGTGTGCCGGAGATGACGCAGCGGGGCCAGGACATTGCCGA
CCGGATCATGGGCGCGGGCGGAGGCCGCTTCGCAGTCCGCGTGGAATCCATCCCGGGCGATGGCAAGCGATTGCAGGCGC
ACCTGACGCTCAGCGACGGCAGCCCGCTGACCATCGATGTGCACCCGCGCATGACGCCGATCGCGCAATGGCTGCCCTAT
GTGCTGGTGATCCAGTTGCTGCTGCTGGTGGTGTGTTGCTGGTTCGCCGTGCGGCTGGCCATCCGCCCGCTGGTGAAGCT
TGCCGATGCCGCCGATGCGCTCGACCCGAACGCGAAGACGCCGCCGCTTGAGGAGAACGGTCCCACCGAACTGGCGCGCG
CGGCCCGTGCGTTCAATGCCATGCGCGAGCGCATCGCCCGGTTTGTGGAGGAGCGCGTGCAGATCCTCGCCGCGATTTCC
CATGATCTGCAGACGCCGATCACGCGCATGAAGCTGCGTGCCGAGATGGCCGACGACTCCGAGGAGAAGCGCAAGATGGT
CAACGACCTCGCTGAGATCGAGACGCTGGTGAAGGAAGGGCTGGCGTATGCGCGCTCGGCGCACGGCGGTGGCGAGAAGA
TGTTACGGATCGACCTGTGTTCGTTTGTGGAAAGCCTGGTGTATGACTATCAGGACACCGGCAAGGACGTCACCGTGCAG
CAGAGCGTTGGCTGTGCGATCGAGACGCGCCCGCAGGCACTGCGCCGCATCCTGACGAACCTGATCGACAACGCGATCAA
GTTCGGCGGCGCGGCGGAACTGAACGTGCGGCGCGATCAGGACGCCGTGGTCATCGAAGTGCTGGACCGCGGTGCCGGCA
TCCCGGAGGACAAGCTCGAATCGGTGCTGCAGCCGTTCGTGCGGCTTGAAAACTCGCGCAGCCGGGAGACCGGCGGCACC
GGGCTGGGCCTGGCGATCGCCCAGCAGCTCGCGGTAGCCATGGGCGGCTCGCTCAGGCTGCGCAATCGCGAAGGGGGCGG
GCTCGCGGCCGAAGTCCGCTTGGGCTGA

Upstream 100 bases:

>100_bases
AGCGCCTCAACGACGATGCGCGCGAACCCAAACATATCAAGACCGTGCGCAACGAGGGCTACGTGTTCTCGATGCCCGTG
GAAATCCGGGAGGCACGCCA

Downstream 100 bases:

>100_bases
TGGCCCTGAGCCACGGCACTTTGTATGCGTTTGTATCGCGCGCCCGCAACGATACGTAAACACGCAAGCGCAGCCTTCAC
GCGCTAAGGGCGCGATACAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTLTLPRWEWPRTLGARLFLILLAGLVVAHGLSFGVLFAERYISARQVMLGTLETDVATSVAILDRLPASERPQWLPRLN
RGNYQYILGPGLPGVPEMTQRGQDIADRIMGAGGGRFAVRVESIPGDGKRLQAHLTLSDGSPLTIDVHPRMTPIAQWLPY
VLVIQLLLLVVCCWFAVRLAIRPLVKLADAADALDPNAKTPPLEENGPTELARAARAFNAMRERIARFVEERVQILAAIS
HDLQTPITRMKLRAEMADDSEEKRKMVNDLAEIETLVKEGLAYARSAHGGGEKMLRIDLCSFVESLVYDYQDTGKDVTVQ
QSVGCAIETRPQALRRILTNLIDNAIKFGGAAELNVRRDQDAVVIEVLDRGAGIPEDKLESVLQPFVRLENSRSRETGGT
GLGLAIAQQLAVAMGGSLRLRNREGGGLAAEVRLG

Sequences:

>Translated_435_residues
MTLTLPRWEWPRTLGARLFLILLAGLVVAHGLSFGVLFAERYISARQVMLGTLETDVATSVAILDRLPASERPQWLPRLN
RGNYQYILGPGLPGVPEMTQRGQDIADRIMGAGGGRFAVRVESIPGDGKRLQAHLTLSDGSPLTIDVHPRMTPIAQWLPY
VLVIQLLLLVVCCWFAVRLAIRPLVKLADAADALDPNAKTPPLEENGPTELARAARAFNAMRERIARFVEERVQILAAIS
HDLQTPITRMKLRAEMADDSEEKRKMVNDLAEIETLVKEGLAYARSAHGGGEKMLRIDLCSFVESLVYDYQDTGKDVTVQ
QSVGCAIETRPQALRRILTNLIDNAIKFGGAAELNVRRDQDAVVIEVLDRGAGIPEDKLESVLQPFVRLENSRSRETGGT
GLGLAIAQQLAVAMGGSLRLRNREGGGLAAEVRLG
>Mature_434_residues
TLTLPRWEWPRTLGARLFLILLAGLVVAHGLSFGVLFAERYISARQVMLGTLETDVATSVAILDRLPASERPQWLPRLNR
GNYQYILGPGLPGVPEMTQRGQDIADRIMGAGGGRFAVRVESIPGDGKRLQAHLTLSDGSPLTIDVHPRMTPIAQWLPYV
LVIQLLLLVVCCWFAVRLAIRPLVKLADAADALDPNAKTPPLEENGPTELARAARAFNAMRERIARFVEERVQILAAISH
DLQTPITRMKLRAEMADDSEEKRKMVNDLAEIETLVKEGLAYARSAHGGGEKMLRIDLCSFVESLVYDYQDTGKDVTVQQ
SVGCAIETRPQALRRILTNLIDNAIKFGGAAELNVRRDQDAVVIEVLDRGAGIPEDKLESVLQPFVRLENSRSRETGGTG
LGLAIAQQLAVAMGGSLRLRNREGGGLAAEVRLG

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=290, Percent_Identity=30.6896551724138, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789808, Length=274, Percent_Identity=29.9270072992701, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790346, Length=259, Percent_Identity=30.1158301158301, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790861, Length=324, Percent_Identity=26.8518518518519, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786783, Length=375, Percent_Identity=26.1333333333333, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=290, Percent_Identity=24.4827586206897, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=297, Percent_Identity=25.2525252525253, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=23.6363636363636, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=313, Percent_Identity=28.4345047923323, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789149, Length=220, Percent_Identity=29.0909090909091, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=26.7241379310345, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787374, Length=297, Percent_Identity=25.2525252525253, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789403, Length=293, Percent_Identity=24.9146757679181, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=29.6137339055794, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788279, Length=319, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=211, Percent_Identity=26.0663507109005, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788713, Length=241, Percent_Identity=23.6514522821577, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47671; Mature: 47540

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTLPRWEWPRTLGARLFLILLAGLVVAHGLSFGVLFAERYISARQVMLGTLETDVATS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAILDRLPASERPQWLPRLNRGNYQYILGPGLPGVPEMTQRGQDIADRIMGAGGGRFAVR
HHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHCCCCCEEEEE
VESIPGDGKRLQAHLTLSDGSPLTIDVHPRMTPIAQWLPYVLVIQLLLLVVCCWFAVRLA
EECCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRPLVKLADAADALDPNAKTPPLEENGPTELARAARAFNAMRERIARFVEERVQILAAIS
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDLQTPITRMKLRAEMADDSEEKRKMVNDLAEIETLVKEGLAYARSAHGGGEKMLRIDLC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SFVESLVYDYQDTGKDVTVQQSVGCAIETRPQALRRILTNLIDNAIKFGGAAELNVRRDQ
HHHHHHHHHHHCCCCCEEEEHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
DAVVIEVLDRGAGIPEDKLESVLQPFVRLENSRSRETGGTGLGLAIAQQLAVAMGGSLRL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
RNREGGGLAAEVRLG
ECCCCCCEEEEEECC
>Mature Secondary Structure 
TLTLPRWEWPRTLGARLFLILLAGLVVAHGLSFGVLFAERYISARQVMLGTLETDVATS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAILDRLPASERPQWLPRLNRGNYQYILGPGLPGVPEMTQRGQDIADRIMGAGGGRFAVR
HHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHCCCCCEEEEE
VESIPGDGKRLQAHLTLSDGSPLTIDVHPRMTPIAQWLPYVLVIQLLLLVVCCWFAVRLA
EECCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRPLVKLADAADALDPNAKTPPLEENGPTELARAARAFNAMRERIARFVEERVQILAAIS
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDLQTPITRMKLRAEMADDSEEKRKMVNDLAEIETLVKEGLAYARSAHGGGEKMLRIDLC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SFVESLVYDYQDTGKDVTVQQSVGCAIETRPQALRRILTNLIDNAIKFGGAAELNVRRDQ
HHHHHHHHHHHCCCCCEEEEHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
DAVVIEVLDRGAGIPEDKLESVLQPFVRLENSRSRETGGTGLGLAIAQQLAVAMGGSLRL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
RNREGGGLAAEVRLG
ECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]