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The map label for this gene is yflS [H]

Identifier: 73537734

GI number: 73537734

Start: 529341

End: 530846

Strand: Reverse

Name: yflS [H]

Synonym: Reut_B3900

Alternate gene names: 73537734

Gene position: 530846-529341 (Counterclockwise)

Preceding gene: 73537735

Following gene: 73537732

Centisome position: 19.47

GC content: 63.21

Gene sequence:

>1506_bases
ATGCTTGCTCAAATCGCTGCGATGTATCGCTACTTCATGTCGGTCGTACCGTTCCGGCTCACGCCCGCCATCATCAGCAC
GGTCGTGCTGGTCACCTTGCTGCTCCTTCCGGCCCCCGAAGGCCTGAAGCCCGAGGCCTGGCATCTGGTCGCCATCTTCC
TGACGACGATCGTGGCCATCATTCTCAAGGTGATGCCGATCGGTGTGATGGCGATGATGGCCATCGTCATCGTCTCGCTG
GCGCAGGTGACGTCGAACTCCTCGAAAGGCGCCATTGCCGACGCGCTCAGCAGCTTCGACAACCCGCTGATCTGGCTCAT
CGTGGTCGCGATCCTGATCTCGCGCGGCCTGAAGAAGACCGGACTTGGCAGCCGCATCGGGCTGATCTTCATCTCGCTGC
TCGGCAAGCGCACGGTGGGCATCGGCTACGGGCTTGCGATCTGCGAACTGGTGCTGGCGCCGTTCACGCCCAGCAATACC
GCGCGCGGCGGCGGCATCGTGCATCCGATCATGAAGTCCATCGCCGGCGCGTTCGACTCGGATCCGGCCAAGGACACGCA
GGGCAAGGTCGGCACCTACCTGGCGCTGGTCAACTACCACGCGAACCCGATCACGTCGGCCATGTTCGTGACCGCCACCG
CGCCGAACCCGCTGGTGGTCGACTATGTCGCCAAGGCCAGTGGCGGTGCGCTGAATCTCAGCTGGACCACCTGGGCCCTG
TGCATGCTGGTGCCAGGCCTGCTCTGTCTGCTGGTGATGCCGTTGATCATCTACGTGCTGCAACCACCCGAACTGAAGGC
GACGCCCAACGCCATCGACTATGCGCGCGGCGAACTGGAGCGCATGGGCCCCCTGAGCGGACAGGAGCGCGTGATGATGG
GTGTCTTCGCCGTGATGCTGGTGCTGTGGGCGAACCTGCCGGCGATGATTTTCGGCCCCGGGCTGACGCTTGATCCGACC
GTGGTCGCCTTCCTCGGCCTGTTTGCGCTCATCATTACCGGCACGATCGACTGGGACGACGTGCTATCCGAGAAAAGCGC
CTGGGATACGCTGATCTGGTTCGGCGCGCTGGTCATGCTCGCCGAGCAACTCAACAAACTCGGGGTCATCACCTGGTTCG
CGGCGGACATGCGCGACGCCATTGAGGCCAGTGGCATGGGCTGGCTCGCAACCGCTGCCGTGCTGGTGCTTGCCTTTGTC
TTTTCGCACTACCTGTTCGCGAGCACCACCGCCCACATCAGCGCCATGATGCTGGCTTTCCTGACCGTGGGCGTGCACCT
GATCCCGCAGCCCTACGTGCCCGTGTTCATGCTCATGATGACGGCCGGATCTGCGATCATGATGACGCTGACCCACTACG
CCACGGGCACGTCCCCGATCATTTTCGGCAGCGGCTACGTGACGCTGGGACAGTGGTGGCGGGTCGGCGCCGTCATGTGC
GTGTTCGAACTGATCGTCTTCGCCGTGGTAGGCGGTATCTGGTGGAAGATCCTGGGATTCTGGTGA

Upstream 100 bases:

>100_bases
TCTGGTCACGTGCCAGGCCAGAGCCCGCGCTTTGCAAGCGCCGCCGGCGCAAGCCCCGGCTGCCTGTATCCACTGCGTTC
CCCGTACGGAGATCCAGAGA

Downstream 100 bases:

>100_bases
TCCTCACCCGATGCGCCGCCGCACAAGAGGGCTGATGCAGCGCGCGCATGGGGAATGGGTTCACTCCAGCGACACCCCCG
TATCCGCCGTGAGCTTCAGT

Product: Sodium/sulfate symporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MLAQIAAMYRYFMSVVPFRLTPAIISTVVLVTLLLLPAPEGLKPEAWHLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSL
AQVTSNSSKGAIADALSSFDNPLIWLIVVAILISRGLKKTGLGSRIGLIFISLLGKRTVGIGYGLAICELVLAPFTPSNT
ARGGGIVHPIMKSIAGAFDSDPAKDTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGGALNLSWTTWAL
CMLVPGLLCLLVMPLIIYVLQPPELKATPNAIDYARGELERMGPLSGQERVMMGVFAVMLVLWANLPAMIFGPGLTLDPT
VVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVITWFAADMRDAIEASGMGWLATAAVLVLAFV
FSHYLFASTTAHISAMMLAFLTVGVHLIPQPYVPVFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTLGQWWRVGAVMC
VFELIVFAVVGGIWWKILGFW

Sequences:

>Translated_501_residues
MLAQIAAMYRYFMSVVPFRLTPAIISTVVLVTLLLLPAPEGLKPEAWHLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSL
AQVTSNSSKGAIADALSSFDNPLIWLIVVAILISRGLKKTGLGSRIGLIFISLLGKRTVGIGYGLAICELVLAPFTPSNT
ARGGGIVHPIMKSIAGAFDSDPAKDTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGGALNLSWTTWAL
CMLVPGLLCLLVMPLIIYVLQPPELKATPNAIDYARGELERMGPLSGQERVMMGVFAVMLVLWANLPAMIFGPGLTLDPT
VVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVITWFAADMRDAIEASGMGWLATAAVLVLAFV
FSHYLFASTTAHISAMMLAFLTVGVHLIPQPYVPVFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTLGQWWRVGAVMC
VFELIVFAVVGGIWWKILGFW
>Mature_501_residues
MLAQIAAMYRYFMSVVPFRLTPAIISTVVLVTLLLLPAPEGLKPEAWHLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSL
AQVTSNSSKGAIADALSSFDNPLIWLIVVAILISRGLKKTGLGSRIGLIFISLLGKRTVGIGYGLAICELVLAPFTPSNT
ARGGGIVHPIMKSIAGAFDSDPAKDTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGGALNLSWTTWAL
CMLVPGLLCLLVMPLIIYVLQPPELKATPNAIDYARGELERMGPLSGQERVMMGVFAVMLVLWANLPAMIFGPGLTLDPT
VVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVITWFAADMRDAIEASGMGWLATAAVLVLAFV
FSHYLFASTTAHISAMMLAFLTVGVHLIPQPYVPVFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTLGQWWRVGAVMC
VFELIVFAVVGGIWWKILGFW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=488, Percent_Identity=35.8606557377049, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1789444, Length=478, Percent_Identity=36.4016736401674, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1786986, Length=483, Percent_Identity=35.1966873706004, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 53788; Mature: 53788

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQIAAMYRYFMSVVPFRLTPAIISTVVLVTLLLLPAPEGLKPEAWHLVAIFLTTIVAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ILKVMPIGVMAMMAIVIVSLAQVTSNSSKGAIADALSSFDNPLIWLIVVAILISRGLKKT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GLGSRIGLIFISLLGKRTVGIGYGLAICELVLAPFTPSNTARGGGIVHPIMKSIAGAFDS
CCCHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCC
DPAKDTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGGALNLSWTTWAL
CCCCCCCCCHHHHHEEEECCCCCCCCEEEEEECCCCCEEEHHHHHCCCCEEECHHHHHHH
CMLVPGLLCLLVMPLIIYVLQPPELKATPNAIDYARGELERMGPLSGQERVMMGVFAVML
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VLWANLPAMIFGPGLTLDPTVVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVML
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEQLNKLGVITWFAADMRDAIEASGMGWLATAAVLVLAFVFSHYLFASTTAHISAMMLAF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVGVHLIPQPYVPVFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTLGQWWRVGAVMC
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHH
VFELIVFAVVGGIWWKILGFW
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAQIAAMYRYFMSVVPFRLTPAIISTVVLVTLLLLPAPEGLKPEAWHLVAIFLTTIVAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ILKVMPIGVMAMMAIVIVSLAQVTSNSSKGAIADALSSFDNPLIWLIVVAILISRGLKKT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GLGSRIGLIFISLLGKRTVGIGYGLAICELVLAPFTPSNTARGGGIVHPIMKSIAGAFDS
CCCHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCC
DPAKDTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGGALNLSWTTWAL
CCCCCCCCCHHHHHEEEECCCCCCCCEEEEEECCCCCEEEHHHHHCCCCEEECHHHHHHH
CMLVPGLLCLLVMPLIIYVLQPPELKATPNAIDYARGELERMGPLSGQERVMMGVFAVML
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VLWANLPAMIFGPGLTLDPTVVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVML
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEQLNKLGVITWFAADMRDAIEASGMGWLATAAVLVLAFVFSHYLFASTTAHISAMMLAF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTVGVHLIPQPYVPVFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTLGQWWRVGAVMC
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHH
VFELIVFAVVGGIWWKILGFW
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]