BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is ligD [H]

Identifier: 73537686

GI number: 73537686

Start: 466360

End: 467193

Strand: Reverse

Name: ligD [H]

Synonym: Reut_B3851

Alternate gene names: 73537686

Gene position: 467193-466360 (Counterclockwise)

Preceding gene: 73537687

Following gene: 73537685

Centisome position: 17.14

GC content: 66.67

Gene sequence:

>834_bases
ATGACTCAATCTGACTCCCGACCGCGCCTTCAGGATTTCGAGGGCAAGACGGCAATCATCACCGGCGCCGCGTCCGGCAT
CGGCCTGGCATTGGCCGAGGCACTGGCAGCGCGCGGTGCCGACCTTGCGCTGGCCGACATTGACGCACGCGGGCTTGACG
CCGCGCGCAAGCGCCTCGCACCGACCGGCCGGCGCATCTGCACGCGCGTGCTGGACGTTTCCTCCGATGCGGACGTGCGC
GACGCGGCGGCGGAGTTCGAAGCGACGCTCGGACGCATCCATCTGGTCTTCAATAACGCTGGCATCGATATGAGCGGCGA
ACTGGCGTCTCTGTCCGAGCAGGACTGGACGCGCGCGTTCGGCATCAATGTGTTTGGCGTCATTCACGGTATCCGCCATT
TCCTGCCGCTGCTGCGCCGGCACGGCGAGCCCGCGCATTTCGTCAGCACGGCATCGGGCGCAGGCTTATGGGTGAACGGC
GACTTCCCCATGGGCGCCTACGCGGCCACGAAATACAGCGTGGTGGCCCTCTCCGAGGCACTTGAGCAGGAGCTTCGCGG
CACCGGCATTGGTGTATCCGTGCTTTGTCCTGGTCCGGTGAAGACGCCGATCGCCGAACGCTCGAACCATGCCAGCGAAT
CGTTCAGGGCCGCAGTGGCCACTGGCACGGCGCCAGAGCTGGTGGCCAGCCACACGCTGGAGGCGATGCGCGCGGGCGAG
TTTTACATTTTCACGCCAACGCGCATGCGGCCGCGCCTGGAGGCGCGATTCGCGCGGATTCTCGATGCGCTGGAGCGCGC
GCCCGCCGTCACAACCGGAATGGCCGCGACATGA

Upstream 100 bases:

>100_bases
AACCGCTTGTTTACCGGCAAGAACATCGGCAAGCAGCTTGTGCGCCTGGCGCCGGAATCCGTCATCGGCTAAATGCCGCG
ACTCTCACCTGTCAATCTCC

Downstream 100 bases:

>100_bases
TCCACCTCATCTTCCTCAGCATGCGGCGCGCCGACGTGGACCGCCGGGAGTACCTGCGCGACTGGCGCGACGTTCAGGCG
CCGCTGGTAGCCGGCCTGCC

Product: Short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTQSDSRPRLQDFEGKTAIITGAASGIGLALAEALAARGADLALADIDARGLDAARKRLAPTGRRICTRVLDVSSDADVR
DAAAEFEATLGRIHLVFNNAGIDMSGELASLSEQDWTRAFGINVFGVIHGIRHFLPLLRRHGEPAHFVSTASGAGLWVNG
DFPMGAYAATKYSVVALSEALEQELRGTGIGVSVLCPGPVKTPIAERSNHASESFRAAVATGTAPELVASHTLEAMRAGE
FYIFTPTRMRPRLEARFARILDALERAPAVTTGMAAT

Sequences:

>Translated_277_residues
MTQSDSRPRLQDFEGKTAIITGAASGIGLALAEALAARGADLALADIDARGLDAARKRLAPTGRRICTRVLDVSSDADVR
DAAAEFEATLGRIHLVFNNAGIDMSGELASLSEQDWTRAFGINVFGVIHGIRHFLPLLRRHGEPAHFVSTASGAGLWVNG
DFPMGAYAATKYSVVALSEALEQELRGTGIGVSVLCPGPVKTPIAERSNHASESFRAAVATGTAPELVASHTLEAMRAGE
FYIFTPTRMRPRLEARFARILDALERAPAVTTGMAAT
>Mature_276_residues
TQSDSRPRLQDFEGKTAIITGAASGIGLALAEALAARGADLALADIDARGLDAARKRLAPTGRRICTRVLDVSSDADVRD
AAAEFEATLGRIHLVFNNAGIDMSGELASLSEQDWTRAFGINVFGVIHGIRHFLPLLRRHGEPAHFVSTASGAGLWVNGD
FPMGAYAATKYSVVALSEALEQELRGTGIGVSVLCPGPVKTPIAERSNHASESFRAAVATGTAPELVASHTLEAMRAGEF
YIFTPTRMRPRLEARFARILDALERAPAVTTGMAAT

Specific function: Catalyzes the C alpha dehydrogenation of arylglycerol- beta-aryl ether (C alpha alcohol type) (compound IV) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=244, Percent_Identity=26.6393442622951, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI31542939, Length=201, Percent_Identity=31.3432835820896, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI210032110, Length=199, Percent_Identity=29.6482412060301, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4758504, Length=202, Percent_Identity=30.6930693069307, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI40807363, Length=250, Percent_Identity=26.4, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI22507405, Length=201, Percent_Identity=30.3482587064677, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI223718074, Length=275, Percent_Identity=30.1818181818182, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI66933014, Length=231, Percent_Identity=30.3030303030303, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI109715829, Length=261, Percent_Identity=27.9693486590038, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI150247226, Length=207, Percent_Identity=32.8502415458937, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4503817, Length=245, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI83715985, Length=188, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19743808, Length=199, Percent_Identity=30.6532663316583, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI225579078, Length=227, Percent_Identity=28.1938325991189, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI7657478, Length=193, Percent_Identity=31.6062176165803, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40548397, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI214010158, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI214010156, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI22758144, Length=203, Percent_Identity=29.064039408867, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI224922801, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI44680136, Length=208, Percent_Identity=30.2884615384615, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI44680133, Length=208, Percent_Identity=30.2884615384615, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI17738292, Length=208, Percent_Identity=30.2884615384615, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI40254992, Length=232, Percent_Identity=27.1551724137931, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI25282469, Length=275, Percent_Identity=24.7272727272727, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI20149619, Length=231, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4504505, Length=191, Percent_Identity=27.7486910994764, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI5031737, Length=201, Percent_Identity=25.8706467661692, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87082100, Length=196, Percent_Identity=36.2244897959184, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786701, Length=196, Percent_Identity=35.7142857142857, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787335, Length=195, Percent_Identity=30.2564102564103, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790717, Length=238, Percent_Identity=27.7310924369748, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786812, Length=193, Percent_Identity=30.5699481865285, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789378, Length=200, Percent_Identity=29.5, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367365, Length=194, Percent_Identity=30.9278350515464, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=204, Percent_Identity=31.3725490196078, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564282, Length=231, Percent_Identity=26.4069264069264, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508895, Length=239, Percent_Identity=24.6861924686192, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=193, Percent_Identity=31.0880829015544, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538182, Length=223, Percent_Identity=29.5964125560538, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=196, Percent_Identity=29.0816326530612, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=195, Percent_Identity=31.7948717948718, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=194, Percent_Identity=26.8041237113402, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507613, Length=223, Percent_Identity=28.6995515695067, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI32563809, Length=223, Percent_Identity=28.6995515695067, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=190, Percent_Identity=26.8421052631579, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=191, Percent_Identity=28.7958115183246, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=196, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356301, Length=244, Percent_Identity=29.5081967213115, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581726, Length=246, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI116007236, Length=208, Percent_Identity=29.3269230769231, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571387, Length=199, Percent_Identity=31.1557788944724, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI23397609, Length=210, Percent_Identity=32.8571428571429, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21355631, Length=182, Percent_Identity=31.3186813186813, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648693, Length=204, Percent_Identity=32.843137254902, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21355889, Length=204, Percent_Identity=32.843137254902, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24641388, Length=203, Percent_Identity=33.4975369458128, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17737361, Length=203, Percent_Identity=29.064039408867, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364892, Length=177, Percent_Identity=31.638418079096, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665243, Length=194, Percent_Identity=28.8659793814433, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221331218, Length=194, Percent_Identity=28.8659793814433, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355319, Length=199, Percent_Identity=26.1306532663317, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116007238, Length=196, Percent_Identity=31.1224489795918, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640492, Length=236, Percent_Identity=26.271186440678, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45554248, Length=209, Percent_Identity=29.6650717703349, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640117, Length=209, Percent_Identity=29.6650717703349, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364494, Length=213, Percent_Identity=30.9859154929577, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665247, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639444, Length=196, Percent_Identity=31.1224489795918, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221331216, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573296, Length=207, Percent_Identity=29.4685990338164, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45553409, Length=204, Percent_Identity=29.9019607843137, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24647948, Length=204, Percent_Identity=29.9019607843137, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24647946, Length=203, Percent_Identity=30.0492610837438, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644339, Length=212, Percent_Identity=28.3018867924528, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921754, Length=206, Percent_Identity=28.1553398058252, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358495, Length=246, Percent_Identity=24.7967479674797, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24584752, Length=208, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI22024069, Length=211, Percent_Identity=28.9099526066351, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI18859837, Length=206, Percent_Identity=24.7572815533981, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 29353; Mature: 29221

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSDSRPRLQDFEGKTAIITGAASGIGLALAEALAARGADLALADIDARGLDAARKRLA
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCC
PTGRRICTRVLDVSSDADVRDAAAEFEATLGRIHLVFNNAGIDMSGELASLSEQDWTRAF
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHCCHHHHHHHH
GINVFGVIHGIRHFLPLLRRHGEPAHFVSTASGAGLWVNGDFPMGAYAATKYSVVALSEA
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
LEQELRGTGIGVSVLCPGPVKTPIAERSNHASESFRAAVATGTAPELVASHTLEAMRAGE
HHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
FYIFTPTRMRPRLEARFARILDALERAPAVTTGMAAT
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TQSDSRPRLQDFEGKTAIITGAASGIGLALAEALAARGADLALADIDARGLDAARKRLA
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCC
PTGRRICTRVLDVSSDADVRDAAAEFEATLGRIHLVFNNAGIDMSGELASLSEQDWTRAF
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHCCHHHHHHHH
GINVFGVIHGIRHFLPLLRRHGEPAHFVSTASGAGLWVNGDFPMGAYAATKYSVVALSEA
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
LEQELRGTGIGVSVLCPGPVKTPIAERSNHASESFRAAVATGTAPELVASHTLEAMRAGE
HHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
FYIFTPTRMRPRLEARFARILDALERAPAVTTGMAAT
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726; 7764263 [H]