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The map label for this gene is mdlB [H]

Identifier: 73537680

GI number: 73537680

Start: 460572

End: 461765

Strand: Direct

Name: mdlB [H]

Synonym: Reut_B3845

Alternate gene names: 73537680

Gene position: 460572-461765 (Clockwise)

Preceding gene: 73537679

Following gene: 73537682

Centisome position: 16.89

GC content: 67.5

Gene sequence:

>1194_bases
ATGACACTGAAACGCAGACTCTATCGCGGCCGCGACGTGCGGCGTGCCCAGAACATTGCCGAGCTGCGCGCGATGGCGTC
AGCGCGGCTGCCCAACTTTTGCCTGGAATACCTGGAGGGCGGGGCGGACGATGAACTGACGCTGCACCGCAACCGCGCCA
GTTATGACGCCATCGCCTTCACGCCGCGCACGCTGGTTGACGTCTCGGTGCGCGATCCCGGCTGCACGCTGTTTGGCCAG
CGCATTGAAATGCCGGCGGTGATTGCGCCAACCGGCTTCAACGGACTGCTCACACACGAGGGTGACCTGGCGCTCGCGCA
TGCCGCGCGTGATGCGGGCATCCCGATGTGCCAGAGCATGGTGTCGACGGTTGCGCTCGAACGCGTTGCCGAAACGGGCG
TACGGCACTGGATGCAGATCTATCCGTTCAAGGACCACGAGAATCTCGCCGGGATCGTCAGGCGTGCCGAGGCGGCGGGC
TCGGAAGCCATCGTCGTGACGACGGACGTGCCGGTGCTTGGCAACCGCGAGTGGGACCGGCGCAACTATCGCGCCCCGAT
GAAGCTGGATGTTGCCAACCTGATCAACGTTGCCCTGCGGCCAAAGTGGTGGTGGGACGTGCTGGTGCCGCACGGCATGC
CGCACTTCCGCAATCTCGGCGACATCCTTCCACCGGGCCAGGACGATGCGCGCAATGCGGCCACCTTCCTGTCCCGGCAG
ATGGATCCCTCGCTGAACTGGCAGGACGTGCAGTGGCTGCGCGATTTGTGGCCGCGCAAGCTCATCCTCAAGGGCATCGT
GCGGCCTGACGATGCGTTGCGCGCCCGCGCGCTTGGTGTCGACGCGCTGGTCATCACCAACCACGGCGGGCGGCAACTCG
ACAGCTGCGTGGCGCCGATCGAGGTGCTTCCTGAGATCCGCGCTGCGGTCGGCCCGGACATGACGCTGATCGTCGACAGC
GGCCTGCGGCGCGGCGGCGATTTCGTCAAGGCGCGCGCGCTCGGGGCCGATGCGGCAATGTCGGGCCGTGCGACGCTGTA
TGGCCTTGCGGCTGGCGGCAGGACCGGGGTTGCGCACGCGCTGGCAATCCTGCGTGCCGAGATCGATCGCACGCTGGGCC
TGCTCGGCTGCCCGGCGCTCACGGATCTGGGGCCGGACTGTGTCAGCAGGCCAGGCGCGATGCGCGGCGCCTAG

Upstream 100 bases:

>100_bases
AGTTTCGCCCCCTCCTTCACGCAGGGGGCGAAGGAGGCGGCGCGCTTCGGATAATCGGAAGGCCCCAACCACGGTGCGCC
AACCGGACCGACTCGTGCCC

Downstream 100 bases:

>100_bases
CGCCTGATTTCCGGTGCTGTCCGCTGGCGGCGCCATGCCGACACGCTGCAGCCGTAGCGCGTGGCAAACCACCTGGAGAA
GGCGCTGAGCGAGGAAAACC

Product: (S)-2-hydroxy-acid oxidase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTLKRRLYRGRDVRRAQNIAELRAMASARLPNFCLEYLEGGADDELTLHRNRASYDAIAFTPRTLVDVSVRDPGCTLFGQ
RIEMPAVIAPTGFNGLLTHEGDLALAHAARDAGIPMCQSMVSTVALERVAETGVRHWMQIYPFKDHENLAGIVRRAEAAG
SEAIVVTTDVPVLGNREWDRRNYRAPMKLDVANLINVALRPKWWWDVLVPHGMPHFRNLGDILPPGQDDARNAATFLSRQ
MDPSLNWQDVQWLRDLWPRKLILKGIVRPDDALRARALGVDALVITNHGGRQLDSCVAPIEVLPEIRAAVGPDMTLIVDS
GLRRGGDFVKARALGADAAMSGRATLYGLAAGGRTGVAHALAILRAEIDRTLGLLGCPALTDLGPDCVSRPGAMRGA

Sequences:

>Translated_397_residues
MTLKRRLYRGRDVRRAQNIAELRAMASARLPNFCLEYLEGGADDELTLHRNRASYDAIAFTPRTLVDVSVRDPGCTLFGQ
RIEMPAVIAPTGFNGLLTHEGDLALAHAARDAGIPMCQSMVSTVALERVAETGVRHWMQIYPFKDHENLAGIVRRAEAAG
SEAIVVTTDVPVLGNREWDRRNYRAPMKLDVANLINVALRPKWWWDVLVPHGMPHFRNLGDILPPGQDDARNAATFLSRQ
MDPSLNWQDVQWLRDLWPRKLILKGIVRPDDALRARALGVDALVITNHGGRQLDSCVAPIEVLPEIRAAVGPDMTLIVDS
GLRRGGDFVKARALGADAAMSGRATLYGLAAGGRTGVAHALAILRAEIDRTLGLLGCPALTDLGPDCVSRPGAMRGA
>Mature_396_residues
TLKRRLYRGRDVRRAQNIAELRAMASARLPNFCLEYLEGGADDELTLHRNRASYDAIAFTPRTLVDVSVRDPGCTLFGQR
IEMPAVIAPTGFNGLLTHEGDLALAHAARDAGIPMCQSMVSTVALERVAETGVRHWMQIYPFKDHENLAGIVRRAEAAGS
EAIVVTTDVPVLGNREWDRRNYRAPMKLDVANLINVALRPKWWWDVLVPHGMPHFRNLGDILPPGQDDARNAATFLSRQM
DPSLNWQDVQWLRDLWPRKLILKGIVRPDDALRARALGVDALVITNHGGRQLDSCVAPIEVLPEIRAAVGPDMTLIVDSG
LRRGGDFVKARALGADAAMSGRATLYGLAAGGRTGVAHALAILRAEIDRTLGLLGCPALTDLGPDCVSRPGAMRGA

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=362, Percent_Identity=36.1878453038674, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI54234014, Length=374, Percent_Identity=33.9572192513369, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI7705393, Length=374, Percent_Identity=33.9572192513369, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1790033, Length=375, Percent_Identity=35.7333333333333, Blast_Score=231, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI193208036, Length=372, Percent_Identity=34.4086021505376, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566217, Length=288, Percent_Identity=32.2916666666667, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6323587, Length=381, Percent_Identity=31.758530183727, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281363140, Length=372, Percent_Identity=33.0645161290323, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI78707190, Length=372, Percent_Identity=33.0645161290323, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI78707188, Length=372, Percent_Identity=33.0645161290323, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 43413; Mature: 43281

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKRRLYRGRDVRRAQNIAELRAMASARLPNFCLEYLEGGADDELTLHRNRASYDAIAF
CCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCEEEE
TPRTLVDVSVRDPGCTLFGQRIEMPAVIAPTGFNGLLTHEGDLALAHAARDAGIPMCQSM
CCCEEEEEEECCCCCCEECCCCCCCEEEECCCCCCEEECCCCEEEEHHHHHCCCHHHHHH
VSTVALERVAETGVRHWMQIYPFKDHENLAGIVRRAEAAGSEAIVVTTDVPVLGNREWDR
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
RNYRAPMKLDVANLINVALRPKWWWDVLVPHGMPHFRNLGDILPPGQDDARNAATFLSRQ
CCCCCCCEECHHHHHHHHCCCCHHEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
MDPSLNWQDVQWLRDLWPRKLILKGIVRPDDALRARALGVDALVITNHGGRQLDSCVAPI
CCCCCCHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
EVLPEIRAAVGPDMTLIVDSGLRRGGDFVKARALGADAAMSGRATLYGLAAGGRTGVAHA
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHH
LAILRAEIDRTLGLLGCPALTDLGPDCVSRPGAMRGA
HHHHHHHHHHHHHHHCCCHHHCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
TLKRRLYRGRDVRRAQNIAELRAMASARLPNFCLEYLEGGADDELTLHRNRASYDAIAF
CHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCEEEE
TPRTLVDVSVRDPGCTLFGQRIEMPAVIAPTGFNGLLTHEGDLALAHAARDAGIPMCQSM
CCCEEEEEEECCCCCCEECCCCCCCEEEECCCCCCEEECCCCEEEEHHHHHCCCHHHHHH
VSTVALERVAETGVRHWMQIYPFKDHENLAGIVRRAEAAGSEAIVVTTDVPVLGNREWDR
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
RNYRAPMKLDVANLINVALRPKWWWDVLVPHGMPHFRNLGDILPPGQDDARNAATFLSRQ
CCCCCCCEECHHHHHHHHCCCCHHEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
MDPSLNWQDVQWLRDLWPRKLILKGIVRPDDALRARALGVDALVITNHGGRQLDSCVAPI
CCCCCCHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
EVLPEIRAAVGPDMTLIVDSGLRRGGDFVKARALGADAAMSGRATLYGLAAGGRTGVAHA
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHH
LAILRAEIDRTLGLLGCPALTDLGPDCVSRPGAMRGA
HHHHHHHHHHHHHHHCCCHHHCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]