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The map label for this gene is gatA [H]

Identifier: 73537603

GI number: 73537603

Start: 365013

End: 366509

Strand: Direct

Name: gatA [H]

Synonym: Reut_B3768

Alternate gene names: 73537603

Gene position: 365013-366509 (Clockwise)

Preceding gene: 73537602

Following gene: 73537604

Centisome position: 13.39

GC content: 68.54

Gene sequence:

>1497_bases
GTGAATCTGCCCGACCGGAGCAAAACCGGCCTTGCGCCGTTGTGGAAGATGGCGCAATGGTTGGCCGATGGCGAGGTATC
GGCGGTGGAACTGGTGCAGGCTTGCGAGGCTGCGTGGCACGCGTGGCATGGAGTCATCAACGCACTCGTGGTGTCTGACT
TCGATGCCGCCCGCGCCGCCGCGCAGGAGAGCGACCGTCGCCGCAGCGCGGGACAGGCGCGTGGGGTCCTCGACGGCGTG
CCGTTCTCCATCAAGGAATCGTTCGACGTGGCTGGCTGGCCGACCACTTGCGGCAACCCGGCTCTTCGCGGCCGCGTGGC
CCGGCGCGATGCGGTAGTGGTGCAGCGTCTGCGCGATGCCGGCGCGATCCTGCTCGGCAAGACCAACGTCCCGCTCGGCC
TGCGGGACTGGCAGAGCTACAACGAGATCTACGGCACGACCCGCAATCCGCACGACCCGTCGCGTACGCCGGGCGGCTCG
TCCGGCGGGAGTGCTGCGGCTGTGTGTGCGGGACTGTCGGCGTTCGATGTCGGTTCGGACATTGGTTCCTCGCTGCGCAA
CCCGGCCCACTACTGCGGCATCTTCTCGCTCAAGCCTAGCCATGGACTGGTGCCGCTGGCCGGCCATGGCACCGGCCCGG
CGCGCTTCGGCGAACAGGACATCAACGTGGCCGGGCCTCTGGCCCGCAGCGCGCGCGATCTTGAACCCGTGCTGCGTGCC
ATTGCCGGCCCGTACGGTGACCAGGAGATGGCATACCGCCTCGAACTGCCCGCGTGCCCGCATCGGCGGCTCGCCGATTT
CCGCGTTGCCGTGCTGCCGAGCCATCCGCAGGCCGAAGCCGACAGCGCAGTCGCCACGCAGATCGAGCAGCTTGGCCATT
GGCTCGCGCAACAAGGCGCGCGCGTGGCATGGCACGCGCGGCCGGACTTCGACGCCGCCGAGCTATGGCATGCCTACGTG
ACGTTGCTGCGCGCGGCCACCTCGCTGCATATGGATGACGCGGCATATGCCGATGCGCTCGACCGCGCGGCCAATGCCGC
CGCAGGCGATGCCTCTTATTCCACGCTGCAATACACCGGTGCAACGCTGAGCCATCGCGACTGGCTGCGCCTGCAGGCTG
CGCGCGAACGTTTCGCGGCCGCGTGGCGCCAGCTCTTTGCCGACCATGACGTGCTGCTGTGTCCTGCCGCGACGACAACC
GCTTTCACGCTGAACGAAGCGGGTGAACCATGGACACGCATGCTTGAGGTGAACGGCCAATCGCAACCGATGACCACGCA
GCTGTTCTGGGCCGGCCATTCCGGGCTGTGCGGGCTGCCTTCGGCCGTCGCGCCGATCGGGCCGGCCTCGAACGGCCTGC
CCGTCGGCGTGCAGATCGTTGCTGGCCGCTACCAGGATCTCACCGCACTGCGGTTCGCCACGCTGCTCGAAGACGCAGGC
TACGCTTTCCGGCCACCGCCGCGGCCGTCCACGCTGCCGGCGAACCTCCACCACTAA

Upstream 100 bases:

>100_bases
AAAGATTGAATATAGTATACCGAAATACATCGCAGTCCCTCCCGTAGTCTCTCCCAACGCCCCCACCGTGCACTGCCACG
GATGACAACAAGGAGTCGAC

Downstream 100 bases:

>100_bases
GACCCCCACACACAACATAGGGAGGGATCATGTTTGACTGGTTCCGCGATTTGTCGCGCATGGAGCGCAAGGGCTTCTAT
GGCGCTTTCCTAGGACATGC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNLPDRSKTGLAPLWKMAQWLADGEVSAVELVQACEAAWHAWHGVINALVVSDFDAARAAAQESDRRRSAGQARGVLDGV
PFSIKESFDVAGWPTTCGNPALRGRVARRDAVVVQRLRDAGAILLGKTNVPLGLRDWQSYNEIYGTTRNPHDPSRTPGGS
SGGSAAAVCAGLSAFDVGSDIGSSLRNPAHYCGIFSLKPSHGLVPLAGHGTGPARFGEQDINVAGPLARSARDLEPVLRA
IAGPYGDQEMAYRLELPACPHRRLADFRVAVLPSHPQAEADSAVATQIEQLGHWLAQQGARVAWHARPDFDAAELWHAYV
TLLRAATSLHMDDAAYADALDRAANAAAGDASYSTLQYTGATLSHRDWLRLQAARERFAAAWRQLFADHDVLLCPAATTT
AFTLNEAGEPWTRMLEVNGQSQPMTTQLFWAGHSGLCGLPSAVAPIGPASNGLPVGVQIVAGRYQDLTALRFATLLEDAG
YAFRPPPRPSTLPANLHH

Sequences:

>Translated_498_residues
MNLPDRSKTGLAPLWKMAQWLADGEVSAVELVQACEAAWHAWHGVINALVVSDFDAARAAAQESDRRRSAGQARGVLDGV
PFSIKESFDVAGWPTTCGNPALRGRVARRDAVVVQRLRDAGAILLGKTNVPLGLRDWQSYNEIYGTTRNPHDPSRTPGGS
SGGSAAAVCAGLSAFDVGSDIGSSLRNPAHYCGIFSLKPSHGLVPLAGHGTGPARFGEQDINVAGPLARSARDLEPVLRA
IAGPYGDQEMAYRLELPACPHRRLADFRVAVLPSHPQAEADSAVATQIEQLGHWLAQQGARVAWHARPDFDAAELWHAYV
TLLRAATSLHMDDAAYADALDRAANAAAGDASYSTLQYTGATLSHRDWLRLQAARERFAAAWRQLFADHDVLLCPAATTT
AFTLNEAGEPWTRMLEVNGQSQPMTTQLFWAGHSGLCGLPSAVAPIGPASNGLPVGVQIVAGRYQDLTALRFATLLEDAG
YAFRPPPRPSTLPANLHH
>Mature_498_residues
MNLPDRSKTGLAPLWKMAQWLADGEVSAVELVQACEAAWHAWHGVINALVVSDFDAARAAAQESDRRRSAGQARGVLDGV
PFSIKESFDVAGWPTTCGNPALRGRVARRDAVVVQRLRDAGAILLGKTNVPLGLRDWQSYNEIYGTTRNPHDPSRTPGGS
SGGSAAAVCAGLSAFDVGSDIGSSLRNPAHYCGIFSLKPSHGLVPLAGHGTGPARFGEQDINVAGPLARSARDLEPVLRA
IAGPYGDQEMAYRLELPACPHRRLADFRVAVLPSHPQAEADSAVATQIEQLGHWLAQQGARVAWHARPDFDAAELWHAYV
TLLRAATSLHMDDAAYADALDRAANAAAGDASYSTLQYTGATLSHRDWLRLQAARERFAAAWRQLFADHDVLLCPAATTT
AFTLNEAGEPWTRMLEVNGQSQPMTTQLFWAGHSGLCGLPSAVAPIGPASNGLPVGVQIVAGRYQDLTALRFATLLEDAG
YAFRPPPRPSTLPANLHH

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=29.0060851926978, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=26.0869565217391, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI166795287, Length=168, Percent_Identity=36.9047619047619, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=27.8787878787879, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17556264, Length=224, Percent_Identity=36.1607142857143, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=241, Percent_Identity=33.195020746888, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=219, Percent_Identity=32.8767123287671, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=471, Percent_Identity=24.203821656051, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556278, Length=352, Percent_Identity=30.3977272727273, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=352, Percent_Identity=30.3977272727273, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538254, Length=181, Percent_Identity=33.1491712707182, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=236, Percent_Identity=31.7796610169492, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=398, Percent_Identity=25.1256281407035, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=176, Percent_Identity=32.3863636363636, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24644968, Length=530, Percent_Identity=27.1698113207547, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=28.8343558282209, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI45550774, Length=250, Percent_Identity=32.8, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=26.7578125, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24648435, Length=250, Percent_Identity=32.8, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24648437, Length=250, Percent_Identity=32.8, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24648441, Length=172, Percent_Identity=37.2093023255814, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648439, Length=172, Percent_Identity=37.2093023255814, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=27.7445109780439, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 53248; Mature: 53248

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPDRSKTGLAPLWKMAQWLADGEVSAVELVQACEAAWHAWHGVINALVVSDFDAARAA
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AQESDRRRSAGQARGVLDGVPFSIKESFDVAGWPTTCGNPALRGRVARRDAVVVQRLRDA
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GAILLGKTNVPLGLRDWQSYNEIYGTTRNPHDPSRTPGGSSGGSAAAVCAGLSAFDVGSD
CEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHCCHH
IGSSLRNPAHYCGIFSLKPSHGLVPLAGHGTGPARFGEQDINVAGPLARSARDLEPVLRA
HHHHHCCCHHHEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
IAGPYGDQEMAYRLELPACPHRRLADFRVAVLPSHPQAEADSAVATQIEQLGHWLAQQGA
HHCCCCCCCEEEEEECCCCCCCHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
RVAWHARPDFDAAELWHAYVTLLRAATSLHMDDAAYADALDRAANAAAGDASYSTLQYTG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECC
ATLSHRDWLRLQAARERFAAAWRQLFADHDVLLCPAATTTAFTLNEAGEPWTRMLEVNGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHEEEECCC
SQPMTTQLFWAGHSGLCGLPSAVAPIGPASNGLPVGVQIVAGRYQDLTALRFATLLEDAG
CCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
YAFRPPPRPSTLPANLHH
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLPDRSKTGLAPLWKMAQWLADGEVSAVELVQACEAAWHAWHGVINALVVSDFDAARAA
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AQESDRRRSAGQARGVLDGVPFSIKESFDVAGWPTTCGNPALRGRVARRDAVVVQRLRDA
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GAILLGKTNVPLGLRDWQSYNEIYGTTRNPHDPSRTPGGSSGGSAAAVCAGLSAFDVGSD
CEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHCCHH
IGSSLRNPAHYCGIFSLKPSHGLVPLAGHGTGPARFGEQDINVAGPLARSARDLEPVLRA
HHHHHCCCHHHEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
IAGPYGDQEMAYRLELPACPHRRLADFRVAVLPSHPQAEADSAVATQIEQLGHWLAQQGA
HHCCCCCCCEEEEEECCCCCCCHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
RVAWHARPDFDAAELWHAYVTLLRAATSLHMDDAAYADALDRAANAAAGDASYSTLQYTG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECC
ATLSHRDWLRLQAARERFAAAWRQLFADHDVLLCPAATTTAFTLNEAGEPWTRMLEVNGQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHEEEECCC
SQPMTTQLFWAGHSGLCGLPSAVAPIGPASNGLPVGVQIVAGRYQDLTALRFATLLEDAG
CCCCEEEEEECCCCCCCCCCHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
YAFRPPPRPSTLPANLHH
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA