Click here to switch to the map view.

The map label for this gene is cobT [H]

Identifier: 73537591

GI number: 73537591

Start: 353270

End: 354991

Strand: Direct

Name: cobT [H]

Synonym: Reut_B3756

Alternate gene names: 73537591

Gene position: 353270-354991 (Clockwise)

Preceding gene: 73537590

Following gene: 73537596

Centisome position: 12.96

GC content: 69.45

Gene sequence:

>1722_bases
ATGAGCGCGGCGGCGGCCGCGCAACGGCTGCGCCGGCGACAGCGGCAGGATGCGCTGTCGGGTGCCGCCGTGCGTGCGCT
CACCGGAGATGCGGCGCTGCATTTCCGCGACGGCCGGCTGTGGCGCGAGATGCGGCCCGTGCCGCAGCATGCGCCGCATC
TGCGCACCGATCCGGACATCGACAGCGCGGCCTGCCTGCGCGGGGCCGCCGATGGCGCCGCGCTGCGCCACGCGCATTCC
GATGCCACGTTGCATCGCAGTCTTTGTCCAGAGGACGCCGTCGAGCGGCTGCTGTTCGAAATGCTGGAACAACTGCGCTG
CGAAACGCGCGTGCCGCGCGGCATGGCCGGCGTCGAAGCCAACTTGCGCCATCGTTTCCTGGCGTGGTCGCGTGCGTTTC
ATCGCTCGGGCCTGACCGAGGGCCAGCTTGGCATCCTTCTCTACACGGTGGCGCAAATTGCTTGGTCACGCCTGAGCGGC
GAGCCCGTACTCGATGAAACCGAGGACTTGATCGAAGCCACGCGCGCCGCGATCGTACCCGTGTTGGGCACATCGCTCGC
GGGCTTGCGCGCGCATCGCCATGACCAGCCTGCGTTCGCCGAGCACGCACTGGCACTGGCCCGCGCGGTGGCGCAGATGG
TCCACAGCGAAGCGCCCAATGCAGGCGAGGACGATGACGCCGCCGCATCCGCCGCGCGCGCCGGGTTTGCGTTGTGGCTC
GATTTCGAAGAAGAGCAACCGGAGACCTTCGCCCTCGCTGACAGCGGCCACAGCCGGGTGCTGGCAGAAGCGAAAGACGG
CTATCGCGTCTTCACCACGCGCTACGACCGCGAGGTGCGTGCCGCCACGCTGGTGCGGCGTGCCTTGCTCGACGAATACC
GCACGCAGCTTGACGCGCGTATCGCGCGTGCCGGCGTCAACGTGGCTCGACTTGCGAGACGCCTGCGTGCCGCGCTGGCA
TTGCCGCGCGTGGACGGCTGGTCCTTTGGCGAGGAAAGTGGGCGTATCGATGGCCGCCGCCTCGCGCAGCTTGTCAGCTC
GCCGGCCGAACGCCGCCTGTTCCGGCTGGAAGCGCACCGGGCCCATGCCGATTGCGTGGTCGGCTTCCTCGTGGACTGCT
CGGGATCGATGAAGGCACGGGCCGAGCCGCTGACGCTGCTGCTGGACTTGCTCACCCGCGCGCTCGACGAAGCTGGCGTC
GCCACCGAGGTGCTCGGTTTCACGACCGGTGCCTGGAATGGCGGCCGCGCCCGCGCCGACTGGCTCGCGCGCGGCCGGCC
CGCGCATCCGGGCCGGCTCAACGAGACCTGCCACATGGTGTTCAAGGACGCTACGCGGAGCTGGCGCCGTTCCCGCACCG
ACATCACGGCCCTGCTCAAGCCTGACCTGTTCCGCGAAGGCATCGACGGCGAGGCCGTGGAATGGGCCTGTGCCCGCCTG
CACGCGACCGGCAAGGCGCGCCGCATCCTGCTGGTGCTGTCGGACGGCAGCCCGATGGATACGGCCACGGGCCAGGCCAA
CGACGCCAGCTACCTCGACAACCACCTGAAGGCCGTCGTCGCGCGGCACGAGTTCTTGCGTGACGTGGAAGTGCTGGGGC
TCGGGGTAGGGCTCGACCTGAGCCCATACTACAGGCATGCGCTGGCGGTGGACCTGTCGCAGCCGGTGGATATGGCGATG
CTCGACGAGGTGGCGAACTTGCTGGCGGCACGGCGGCGTTAA

Upstream 100 bases:

>100_bases
GGCCGGTGGTGGCGGAGTACTACCAGCGCTGCTTCGGCAACCTGCCCGGTGAAGCGGCGGGGCAGGCGGCCAGCAACGCG
ACCCGACCGGAGCCGGCGCA

Downstream 100 bases:

>100_bases
GCCAGCCGCACCGATGGGGCTCAGGCGCTCGACGAACACTCGCTCTGCGGGGCAGGGGTATTTGCACCCGCCTGCGCAGG
GCCGCGCGCCATCTCACGCG

Product: CobT protein

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSAAAAAQRLRRRQRQDALSGAAVRALTGDAALHFRDGRLWREMRPVPQHAPHLRTDPDIDSAACLRGAADGAALRHAHS
DATLHRSLCPEDAVERLLFEMLEQLRCETRVPRGMAGVEANLRHRFLAWSRAFHRSGLTEGQLGILLYTVAQIAWSRLSG
EPVLDETEDLIEATRAAIVPVLGTSLAGLRAHRHDQPAFAEHALALARAVAQMVHSEAPNAGEDDDAAASAARAGFALWL
DFEEEQPETFALADSGHSRVLAEAKDGYRVFTTRYDREVRAATLVRRALLDEYRTQLDARIARAGVNVARLARRLRAALA
LPRVDGWSFGEESGRIDGRRLAQLVSSPAERRLFRLEAHRAHADCVVGFLVDCSGSMKARAEPLTLLLDLLTRALDEAGV
ATEVLGFTTGAWNGGRARADWLARGRPAHPGRLNETCHMVFKDATRSWRRSRTDITALLKPDLFREGIDGEAVEWACARL
HATGKARRILLVLSDGSPMDTATGQANDASYLDNHLKAVVARHEFLRDVEVLGLGVGLDLSPYYRHALAVDLSQPVDMAM
LDEVANLLAARRR

Sequences:

>Translated_573_residues
MSAAAAAQRLRRRQRQDALSGAAVRALTGDAALHFRDGRLWREMRPVPQHAPHLRTDPDIDSAACLRGAADGAALRHAHS
DATLHRSLCPEDAVERLLFEMLEQLRCETRVPRGMAGVEANLRHRFLAWSRAFHRSGLTEGQLGILLYTVAQIAWSRLSG
EPVLDETEDLIEATRAAIVPVLGTSLAGLRAHRHDQPAFAEHALALARAVAQMVHSEAPNAGEDDDAAASAARAGFALWL
DFEEEQPETFALADSGHSRVLAEAKDGYRVFTTRYDREVRAATLVRRALLDEYRTQLDARIARAGVNVARLARRLRAALA
LPRVDGWSFGEESGRIDGRRLAQLVSSPAERRLFRLEAHRAHADCVVGFLVDCSGSMKARAEPLTLLLDLLTRALDEAGV
ATEVLGFTTGAWNGGRARADWLARGRPAHPGRLNETCHMVFKDATRSWRRSRTDITALLKPDLFREGIDGEAVEWACARL
HATGKARRILLVLSDGSPMDTATGQANDASYLDNHLKAVVARHEFLRDVEVLGLGVGLDLSPYYRHALAVDLSQPVDMAM
LDEVANLLAARRR
>Mature_572_residues
SAAAAAQRLRRRQRQDALSGAAVRALTGDAALHFRDGRLWREMRPVPQHAPHLRTDPDIDSAACLRGAADGAALRHAHSD
ATLHRSLCPEDAVERLLFEMLEQLRCETRVPRGMAGVEANLRHRFLAWSRAFHRSGLTEGQLGILLYTVAQIAWSRLSGE
PVLDETEDLIEATRAAIVPVLGTSLAGLRAHRHDQPAFAEHALALARAVAQMVHSEAPNAGEDDDAAASAARAGFALWLD
FEEEQPETFALADSGHSRVLAEAKDGYRVFTTRYDREVRAATLVRRALLDEYRTQLDARIARAGVNVARLARRLRAALAL
PRVDGWSFGEESGRIDGRRLAQLVSSPAERRLFRLEAHRAHADCVVGFLVDCSGSMKARAEPLTLLLDLLTRALDEAGVA
TEVLGFTTGAWNGGRARADWLARGRPAHPGRLNETCHMVFKDATRSWRRSRTDITALLKPDLFREGIDGEAVEWACARLH
ATGKARRILLVLSDGSPMDTATGQANDASYLDNHLKAVVARHEFLRDVEVLGLGVGLDLSPYYRHALAVDLSQPVDMAML
DEVANLLAARRR

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 62983; Mature: 62852

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAAAAQRLRRRQRQDALSGAAVRALTGDAALHFRDGRLWREMRPVPQHAPHLRTDPDI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCC
DSAACLRGAADGAALRHAHSDATLHRSLCPEDAVERLLFEMLEQLRCETRVPRGMAGVEA
CHHHHHHCCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
NLRHRFLAWSRAFHRSGLTEGQLGILLYTVAQIAWSRLSGEPVLDETEDLIEATRAAIVP
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VLGTSLAGLRAHRHDQPAFAEHALALARAVAQMVHSEAPNAGEDDDAAASAARAGFALWL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEE
DFEEEQPETFALADSGHSRVLAEAKDGYRVFTTRYDREVRAATLVRRALLDEYRTQLDAR
ECCCCCCCEEEEECCCCCEEEEECCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHH
IARAGVNVARLARRLRAALALPRVDGWSFGEESGRIDGRRLAQLVSSPAERRLFRLEAHR
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
AHADCVVGFLVDCSGSMKARAEPLTLLLDLLTRALDEAGVATEVLGFTTGAWNGGRARAD
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHH
WLARGRPAHPGRLNETCHMVFKDATRSWRRSRTDITALLKPDLFREGIDGEAVEWACARL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHH
HATGKARRILLVLSDGSPMDTATGQANDASYLDNHLKAVVARHEFLRDVEVLGLGVGLDL
HCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
SPYYRHALAVDLSQPVDMAMLDEVANLLAARRR
CHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAAAAAQRLRRRQRQDALSGAAVRALTGDAALHFRDGRLWREMRPVPQHAPHLRTDPDI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCC
DSAACLRGAADGAALRHAHSDATLHRSLCPEDAVERLLFEMLEQLRCETRVPRGMAGVEA
CHHHHHHCCCCCHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
NLRHRFLAWSRAFHRSGLTEGQLGILLYTVAQIAWSRLSGEPVLDETEDLIEATRAAIVP
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
VLGTSLAGLRAHRHDQPAFAEHALALARAVAQMVHSEAPNAGEDDDAAASAARAGFALWL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEE
DFEEEQPETFALADSGHSRVLAEAKDGYRVFTTRYDREVRAATLVRRALLDEYRTQLDAR
ECCCCCCCEEEEECCCCCEEEEECCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHH
IARAGVNVARLARRLRAALALPRVDGWSFGEESGRIDGRRLAQLVSSPAERRLFRLEAHR
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
AHADCVVGFLVDCSGSMKARAEPLTLLLDLLTRALDEAGVATEVLGFTTGAWNGGRARAD
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHH
WLARGRPAHPGRLNETCHMVFKDATRSWRRSRTDITALLKPDLFREGIDGEAVEWACARL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHH
HATGKARRILLVLSDGSPMDTATGQANDASYLDNHLKAVVARHEFLRDVEVLGLGVGLDL
HCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
SPYYRHALAVDLSQPVDMAMLDEVANLLAARRR
CHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]