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The map label for this gene is yafC [C]

Identifier: 73537527

GI number: 73537527

Start: 277757

End: 278674

Strand: Direct

Name: yafC [C]

Synonym: Reut_B3692

Alternate gene names: 73537527

Gene position: 277757-278674 (Clockwise)

Preceding gene: 73537525

Following gene: 73537529

Centisome position: 10.19

GC content: 65.47

Gene sequence:

>918_bases
ATGCCGAAACTTCCTGATTTCGAAGGACTCGCGATGTTCGCGAAGGTCGCCGAGGAGGGATCGTTCACGGCCGCGGCGCG
CGTCATGGGGGTGTCTGTGGCCACGGTCTCGCGTGGCGTCGCCCGGCTGGAAGAACGGCTGGGCGCTCGGCTTTTCAACC
GCACATCACGCGAGCTCTCGCTTACCGGGTTCGGCCAGACGATCTGTGACAAGGCAGGAGCGATCTACCGTCAGGCCGAA
GAAGCGGAAAGCGCCGCGCGCGAGATGTCGGTGCAGCCTCGCGGGCTGGTGCGCCTGGCTGTGCCGATGTCGTTCGGGCT
GCGCTGGGTCGCGCCGCTACTGCCCGGCTTCTTTCGCGCGTACCCGGAGGTGTCGATCGACATGCATCTTTCAGATGCGT
CGGTCGATCTGGTTGCCGATGGGTATGACGCCGCACTGCGCATCGCCGCGCTGCCGGATTCGTCGTTGGTCGTTCGACGG
CTGTGCGCAGTCACACAATTCGTAGTGGCGTCGCCGGCGTATCTGAAGCGCGAAGGGCGGCCCGCCCATCCGCGCGAACT
GGCGGACCGGTCGTGCCTCTCATACGCGTACCGCGCACGCAGCGATGTGTGGCGGTTCACGAACGCTGTGGGCGAGGAGG
AGTCGGTCATGCCCACCGGCCCGTTGCGCGTGACGAATTCGGAGGCGCTGCTGCCCACGTTGCTCGACGGCCTTGCCATT
GCCGAACTCCCCGAATTTATCGCCGGCGAATATTTGGCCGATGGCCGCCTCGAAACGATCCTCACCGACTGGTCGCTGAC
GAAGGGCGGCCTTTACTTCGTCACGCCATCGGCGCGCGCGCGTCCCGCGAAGGTGGCTGCGCTGTCCGACTATTTTGCGG
AGCATCTCTCCGAGCCGACATGGCGATGGCCGAGGTAA

Upstream 100 bases:

>100_bases
TGGTTGCGTGCTGCGATGTGAGGAGAATATCGACGCCCTCGGCTTTTCGAAATAGAATTGATTGCAAACTAACGTTGCAA
AAATGAAAAGATGAGGGGCA

Downstream 100 bases:

>100_bases
TGCGTTGGCGAAGTTGAGCCGCTTTTATGTTCACCGGGGAAATACTTTAGCCGACAACGGCGGCAAGAGCGCTACCCGGG
CCGCCGCCAGCACCTCGCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MPKLPDFEGLAMFAKVAEEGSFTAAARVMGVSVATVSRGVARLEERLGARLFNRTSRELSLTGFGQTICDKAGAIYRQAE
EAESAAREMSVQPRGLVRLAVPMSFGLRWVAPLLPGFFRAYPEVSIDMHLSDASVDLVADGYDAALRIAALPDSSLVVRR
LCAVTQFVVASPAYLKREGRPAHPRELADRSCLSYAYRARSDVWRFTNAVGEEESVMPTGPLRVTNSEALLPTLLDGLAI
AELPEFIAGEYLADGRLETILTDWSLTKGGLYFVTPSARARPAKVAALSDYFAEHLSEPTWRWPR

Sequences:

>Translated_305_residues
MPKLPDFEGLAMFAKVAEEGSFTAAARVMGVSVATVSRGVARLEERLGARLFNRTSRELSLTGFGQTICDKAGAIYRQAE
EAESAAREMSVQPRGLVRLAVPMSFGLRWVAPLLPGFFRAYPEVSIDMHLSDASVDLVADGYDAALRIAALPDSSLVVRR
LCAVTQFVVASPAYLKREGRPAHPRELADRSCLSYAYRARSDVWRFTNAVGEEESVMPTGPLRVTNSEALLPTLLDGLAI
AELPEFIAGEYLADGRLETILTDWSLTKGGLYFVTPSARARPAKVAALSDYFAEHLSEPTWRWPR
>Mature_304_residues
PKLPDFEGLAMFAKVAEEGSFTAAARVMGVSVATVSRGVARLEERLGARLFNRTSRELSLTGFGQTICDKAGAIYRQAEE
AESAAREMSVQPRGLVRLAVPMSFGLRWVAPLLPGFFRAYPEVSIDMHLSDASVDLVADGYDAALRIAALPDSSLVVRRL
CAVTQFVVASPAYLKREGRPAHPRELADRSCLSYAYRARSDVWRFTNAVGEEESVMPTGPLRVTNSEALLPTLLDGLAIA
ELPEFIAGEYLADGRLETILTDWSLTKGGLYFVTPSARARPAKVAALSDYFAEHLSEPTWRWPR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=32.7586206896552, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=28.3276450511945, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=29.757785467128, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87081978, Length=300, Percent_Identity=31.3333333333333, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=28.6689419795222, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789440, Length=263, Percent_Identity=28.5171102661597, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=29.0657439446367, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=24.6428571428571, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=27.3469387755102, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=128, Percent_Identity=33.59375, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790011, Length=272, Percent_Identity=25, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788706, Length=204, Percent_Identity=26.9607843137255, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=29.2682926829268, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33399; Mature: 33267

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLPDFEGLAMFAKVAEEGSFTAAARVMGVSVATVSRGVARLEERLGARLFNRTSRELS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTGFGQTICDKAGAIYRQAEEAESAAREMSVQPRGLVRLAVPMSFGLRWVAPLLPGFFRA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
YPEVSIDMHLSDASVDLVADGYDAALRIAALPDSSLVVRRLCAVTQFVVASPAYLKREGR
CCCCEEEEEECCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHCCCC
PAHPRELADRSCLSYAYRARSDVWRFTNAVGEEESVMPTGPLRVTNSEALLPTLLDGLAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
AELPEFIAGEYLADGRLETILTDWSLTKGGLYFVTPSARARPAKVAALSDYFAEHLSEPT
HHHHHHHHHHHHCCCCHHEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
WRWPR
CCCCC
>Mature Secondary Structure 
PKLPDFEGLAMFAKVAEEGSFTAAARVMGVSVATVSRGVARLEERLGARLFNRTSRELS
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTGFGQTICDKAGAIYRQAEEAESAAREMSVQPRGLVRLAVPMSFGLRWVAPLLPGFFRA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
YPEVSIDMHLSDASVDLVADGYDAALRIAALPDSSLVVRRLCAVTQFVVASPAYLKREGR
CCCCEEEEEECCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHCCCC
PAHPRELADRSCLSYAYRARSDVWRFTNAVGEEESVMPTGPLRVTNSEALLPTLLDGLAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
AELPEFIAGEYLADGRLETILTDWSLTKGGLYFVTPSARARPAKVAALSDYFAEHLSEPT
HHHHHHHHHHHHCCCCHHEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
WRWPR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]