Click here to switch to the map view.

The map label for this gene is garD [H]

Identifier: 73537525

GI number: 73537525

Start: 274948

End: 276522

Strand: Direct

Name: garD [H]

Synonym: Reut_B3690

Alternate gene names: 73537525

Gene position: 274948-276522 (Clockwise)

Preceding gene: 73537524

Following gene: 73537527

Centisome position: 10.09

GC content: 66.41

Gene sequence:

>1575_bases
ATGTCAGAACACGATTCGCAGGCAGTCAAGGCGCCGCTCTATATCCGCATCCATCCCGAGGACAACGTGGCCATCGTCGC
GAACGACGGCGGCCTGCCTGCGGGCGCGGCGTTTCCATGCGGGCTGGTCCTGCAAGAGGCCGTGCCGCAGGGGCACAAGG
TCGCGCTGTCCGACCTGAAGCAGGGCGATGCAGTGGTGCGCTACAACGTGGTGATCGGCTACGCGCTGAAGGACCTGCCG
CGCGGCAGCTGGGTCAACGAGCGCGTCATTGAAATGCCGGTGGCGCCGGGCCTGACCGACCTGCCAATCGGCACGCGCGT
GCCCGCGCCGCTACCGCCGCTGGAAGGCTATACCTTCGAAGGTTTCCGCAATCCGGATGGTTCGGTCGGCACGCGCAACA
TCCTGGCTATTACCACCACCGTGCAATGCGTGTCGGGCGTGGTCGAGCATGCCGTGCGGCGCATTCGCGAAGAACTGTTG
CCGCGCTATCCGAATGTCGATGATGTCGTCGGGCTCGAACACACGTATGGGTGCGGCGTGGCCATCGATGCGCCCGATGC
CGTGATCCCGATCCGCACGCTGCGCAATATCGCGCTCAATCCGAACTTCGGCGGCACCGCGATGATGGTGAGCCTCGGCT
GCGAAAAGCTGCAGCCGGACCGCCTGATGCCGGCGGGCACCATTCCGATCCAGACCGGCGCGGGCGAGGTGCAGGTGGGC
GTGGTCTGCCTGCAGGACGAGCAGCATGTCGGGTTTGCCTCGATGATCGCATCGATCATGACCATGGCCGAAACGCATCT
GGAGCGCCTGAACGCGCGTCGGCGCGAAACCTGCCCGGTGTCGGACCTGGTGGTCGGCGTGCAGTGCGGCGGCAGCGACG
CGTTCTCCGGCGTGACGGCCAACCCCGCAGTCGGCTTCGCCACTGACCTGCTGGTGCGCGCGGGCGCCGCCGTCATGTTC
TCCGAGGTGACCGAAGTGCGCGATGGCATCGACCAGCTCACCGCGCGCGCCGCCACCCCAGAGGTCGCCGACGCGATGAT
CCGCGAAATGGCCTGGTATGACCGCTACCTGGAACGCGGCCGCGTGGACCGCAGCGCCAACACCACACCCGGCAACAAGA
AGGGCGGACTGTCAAACATCGTCGAGAAGGCGATGGGCTCGATCATCAAGTCAGGCAGAGCACCGATCTCGGGCGTGCTG
TCGCCGGGCGAGAAACTGAAGCAGAAGGGATTGATCTACGCGGCCACGCCCGCAAGCGATTTCATCTGCGGCACGCTGCA
GCTTGCGGCCGGCATGAACCTGCACGTGTTCACGACCGGACGCGGCACGCCCTACGGGCTGGCCGAAGTGCCCGTGATCA
AGGTGGCCACGCGCAGCGACCTGGCGCGCCGCTGGCACGATCTGATGGACGTCAACGCGGGCCGCATCGCGACCGGCGAA
GCCACCATCGAGGACGTGGGCTGGGAGCTGTTCCGCCTGATGATCGACGTGGCCAGCGGCCGCAAGAAGACCTGGGCGGA
GCACCACAAGCTGCATAACGCGCTGGCGTTGTTCAATCCGGCGCCGGTGACCTGA

Upstream 100 bases:

>100_bases
GAGTCCATGCACTGGTCACGGTGCTGAGCTACCTGGTGATCGTCAAGGACATTCGCCGCGTGGAACTCAGGCACCCGCAA
TAACCGCAGGAAGCACAAGC

Downstream 100 bases:

>100_bases
GGGCGGATTCGACTCCGCTCCCGGGCGGCTGTCAGCTGAAGTGATTCGCTGGACGATTTGGCTCTATCACTGGTGCTGGC
CTGCTAGGGCTATGCGCCCC

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSEHDSQAVKAPLYIRIHPEDNVAIVANDGGLPAGAAFPCGLVLQEAVPQGHKVALSDLKQGDAVVRYNVVIGYALKDLP
RGSWVNERVIEMPVAPGLTDLPIGTRVPAPLPPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVRRIREELL
PRYPNVDDVVGLEHTYGCGVAIDAPDAVIPIRTLRNIALNPNFGGTAMMVSLGCEKLQPDRLMPAGTIPIQTGAGEVQVG
VVCLQDEQHVGFASMIASIMTMAETHLERLNARRRETCPVSDLVVGVQCGGSDAFSGVTANPAVGFATDLLVRAGAAVMF
SEVTEVRDGIDQLTARAATPEVADAMIREMAWYDRYLERGRVDRSANTTPGNKKGGLSNIVEKAMGSIIKSGRAPISGVL
SPGEKLKQKGLIYAATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGRIATGE
ATIEDVGWELFRLMIDVASGRKKTWAEHHKLHNALALFNPAPVT

Sequences:

>Translated_524_residues
MSEHDSQAVKAPLYIRIHPEDNVAIVANDGGLPAGAAFPCGLVLQEAVPQGHKVALSDLKQGDAVVRYNVVIGYALKDLP
RGSWVNERVIEMPVAPGLTDLPIGTRVPAPLPPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVRRIREELL
PRYPNVDDVVGLEHTYGCGVAIDAPDAVIPIRTLRNIALNPNFGGTAMMVSLGCEKLQPDRLMPAGTIPIQTGAGEVQVG
VVCLQDEQHVGFASMIASIMTMAETHLERLNARRRETCPVSDLVVGVQCGGSDAFSGVTANPAVGFATDLLVRAGAAVMF
SEVTEVRDGIDQLTARAATPEVADAMIREMAWYDRYLERGRVDRSANTTPGNKKGGLSNIVEKAMGSIIKSGRAPISGVL
SPGEKLKQKGLIYAATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGRIATGE
ATIEDVGWELFRLMIDVASGRKKTWAEHHKLHNALALFNPAPVT
>Mature_523_residues
SEHDSQAVKAPLYIRIHPEDNVAIVANDGGLPAGAAFPCGLVLQEAVPQGHKVALSDLKQGDAVVRYNVVIGYALKDLPR
GSWVNERVIEMPVAPGLTDLPIGTRVPAPLPPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVSGVVEHAVRRIREELLP
RYPNVDDVVGLEHTYGCGVAIDAPDAVIPIRTLRNIALNPNFGGTAMMVSLGCEKLQPDRLMPAGTIPIQTGAGEVQVGV
VCLQDEQHVGFASMIASIMTMAETHLERLNARRRETCPVSDLVVGVQCGGSDAFSGVTANPAVGFATDLLVRAGAAVMFS
EVTEVRDGIDQLTARAATPEVADAMIREMAWYDRYLERGRVDRSANTTPGNKKGGLSNIVEKAMGSIIKSGRAPISGVLS
PGEKLKQKGLIYAATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGRIATGEA
TIEDVGWELFRLMIDVASGRKKTWAEHHKLHNALALFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=518, Percent_Identity=63.7065637065637, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=531, Percent_Identity=30.6967984934087, Blast_Score=200, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 55979; Mature: 55848

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHDSQAVKAPLYIRIHPEDNVAIVANDGGLPAGAAFPCGLVLQEAVPQGHKVALSDLK
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHC
QGDAVVRYNVVIGYALKDLPRGSWVNERVIEMPVAPGLTDLPIGTRVPAPLPPLEGYTFE
CCCEEEEEEEEEEEHHHHCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCEEC
GFRNPDGSVGTRNILAITTTVQCVSGVVEHAVRRIREELLPRYPNVDDVVGLEHTYGCGV
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
AIDAPDAVIPIRTLRNIALNPNFGGTAMMVSLGCEKLQPDRLMPAGTIPIQTGAGEVQVG
EECCCCCEEEHHHHHHEECCCCCCCHHEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEE
VVCLQDEQHVGFASMIASIMTMAETHLERLNARRRETCPVSDLVVGVQCGGSDAFSGVTA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCC
NPAVGFATDLLVRAGAAVMFSEVTEVRDGIDQLTARAATPEVADAMIREMAWYDRYLERG
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RVDRSANTTPGNKKGGLSNIVEKAMGSIIKSGRAPISGVLSPGEKLKQKGLIYAATPASD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHCCCEEEECCCCC
FICGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGRIATGE
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEECCC
ATIEDVGWELFRLMIDVASGRKKTWAEHHKLHNALALFNPAPVT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEHDSQAVKAPLYIRIHPEDNVAIVANDGGLPAGAAFPCGLVLQEAVPQGHKVALSDLK
CCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHC
QGDAVVRYNVVIGYALKDLPRGSWVNERVIEMPVAPGLTDLPIGTRVPAPLPPLEGYTFE
CCCEEEEEEEEEEEHHHHCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCEEC
GFRNPDGSVGTRNILAITTTVQCVSGVVEHAVRRIREELLPRYPNVDDVVGLEHTYGCGV
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
AIDAPDAVIPIRTLRNIALNPNFGGTAMMVSLGCEKLQPDRLMPAGTIPIQTGAGEVQVG
EECCCCCEEEHHHHHHEECCCCCCCHHEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEE
VVCLQDEQHVGFASMIASIMTMAETHLERLNARRRETCPVSDLVVGVQCGGSDAFSGVTA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCCCC
NPAVGFATDLLVRAGAAVMFSEVTEVRDGIDQLTARAATPEVADAMIREMAWYDRYLERG
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RVDRSANTTPGNKKGGLSNIVEKAMGSIIKSGRAPISGVLSPGEKLKQKGLIYAATPASD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHCCCEEEECCCCC
FICGTLQLAAGMNLHVFTTGRGTPYGLAEVPVIKVATRSDLARRWHDLMDVNAGRIATGE
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCEEECCC
ATIEDVGWELFRLMIDVASGRKKTWAEHHKLHNALALFNPAPVT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]