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The map label for this gene is ilvB [H]

Identifier: 73537410

GI number: 73537410

Start: 130261

End: 131967

Strand: Direct

Name: ilvB [H]

Synonym: Reut_B3575

Alternate gene names: 73537410

Gene position: 130261-131967 (Clockwise)

Preceding gene: 73537409

Following gene: 73537411

Centisome position: 4.78

GC content: 67.37

Gene sequence:

>1707_bases
ATGCCTAATCAACAACAGTACGAACAACAGCAAGTCACCGTGGGCTGTGCCATCACGGCGTTTCTCGAACAGTGCGGCGT
CAAGGCAGCCTTTGGCGTGATCTCGATCCACAACATGCCGATCCTCGACGCCATGGGCGAGCGCGGCCACATCCGCTTCG
TGCCGGCACGCGGCGAAGCGGGCGGCACCAATATGGCTGACGCCTGCGCCCGTACCACGGGCGGCCTGGGCGTGTGCCTG
ACCAGCACCGGCACCGCCGCGGGCAATGCCGCCGGCGCCATGGTCGAAGCGCTGACCGCCGGCACACCGATGCTGCACAT
CACCGGCCAGATCGAAACGCCATACCTGGATCAGAGCCTGGCGTACATCCACGAAGCACCGGACCAGCTGACCATGCTGA
AGGCCGTGTCGAAGGCCGCCTTCCGCGTGCGCAGCGCCGACACCGCGATCAGCACGATCAAGCTGGCCGTGCAGACCGCG
CTGACGGCGCCGACCGGCCCGGTCAGCGTCGAGATCCCCATCGACATCCAGGCCGCGCTGATCGACATGCCCGTCGACCT
CAAGCCGCTGCCGGTGCCGCAGCACCAGCCGTCGGCGCACGCCATCGACGCGCTGGCCGAGCGCCTCGCCAACGCTAAGC
GCCCGATGCTGTGGCTGGGCGGTGGCGCTCGCCACGCCAGCAAGCAGGTCAAGCGCCTGCTGGACCTGGGCTTCGGCGTG
GTCACCAGCACGCAAGGCCGCGGCATCGTCGCGGAAGACGATCCGCGCTCGCTGGGCGCCTACAACCTGCACAAGCCGGT
CGAGCAGTTCTACCAAAGCTGCGACGCGATGCTCGTGGTCGGCTCGCGCCTGCGTGGCAATGAAACGCTGAAGTACGAAC
TGAAGCTGCCGCGCCCGCTGCTGCGCATCGATGCCGATCCCGCTTCGGAAGGCCGCTGCTACCAGAGCGATTACTTTGTC
AGCGGCGACGCTGCCCTGGCGCTGGACGCCCTGGCTGACCGCCTCGAAGGCCGCATGCAGATCGACCCGACCTTCGCCGA
TGACCTGCGCCGCGCGCACGACACCGCGGTCAACGCGCTGATTGACGGCCTGGGCCCGTACTCGGCACTGGTGCAGGCAC
TGCAGCAGGCCGTGGGCCGCAACTTCAACTGGGTGCGCGACGTGACCGTGTCGAACAGCACCTGGGGCAACCGCCACCTG
CAGATTTTTGATTCGCGTGCCGGCGTGCATGCGCTGGGCGGCGGCATCGGCCAGGGCTTGGCCATGGGTATCGGCGCTGC
AGTCGGTGCGGCGGCCACGGCTTCGGGCAAGAAGACCTTCACGCTGGCTGGCGACGGCGGCTTCATCCTGAACCTCGGCG
AGCTGGCCACGGCCGTGCAGGAACGCGCCGACATGGTCATCGTGCTGATGAACGACAAGGGCTATGGCGTGATCAAGAAC
ATCCAGGACGCGCAGTACGGCGGCCGTCGGCACTATGTCGACCTGCACACGCCCGACTATGCGCAACTGTGCCAGTCGCT
GTCGCTGCGCCACCGCCGCGTCAGCAGCCTGGACGACATCGGCGCCGCGCTGCAGGAAGCCACGGCCGAGACTGGTCCGT
TCCTGCTGGAAATCGACATGCTGTCGATCGGCAGCTTCAAGACCGTGTTTGCCGGCCCGCCGGTGAACAAGCAGGCGCCG
ATTGCTGCGCCGGCTACGACCGCCTGA

Upstream 100 bases:

>100_bases
CTGCCCGTCGAGGCAGCCCGCGCCATCCTGTTCCTTGCCTCGCCGTTGTCTTCGTACACCACGGGCAGCCATATTGATAT
CTCCGGAGGACACTCCCGTC

Downstream 100 bases:

>100_bases
GGGATAAGGGAGATGTCCATGCTGCATGTGTCCATGGTGGGATGCGGCGCGATCGGCCGTGGCGTGCTGGAGCTGCTGAA
GGCGGATCCCGATGTCGCGT

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MPNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGHIRFVPARGEAGGTNMADACARTTGGLGVCL
TSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSLAYIHEAPDQLTMLKAVSKAAFRVRSADTAISTIKLAVQTA
LTAPTGPVSVEIPIDIQAALIDMPVDLKPLPVPQHQPSAHAIDALAERLANAKRPMLWLGGGARHASKQVKRLLDLGFGV
VTSTQGRGIVAEDDPRSLGAYNLHKPVEQFYQSCDAMLVVGSRLRGNETLKYELKLPRPLLRIDADPASEGRCYQSDYFV
SGDAALALDALADRLEGRMQIDPTFADDLRRAHDTAVNALIDGLGPYSALVQALQQAVGRNFNWVRDVTVSNSTWGNRHL
QIFDSRAGVHALGGGIGQGLAMGIGAAVGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
IQDAQYGGRRHYVDLHTPDYAQLCQSLSLRHRRVSSLDDIGAALQEATAETGPFLLEIDMLSIGSFKTVFAGPPVNKQAP
IAAPATTA

Sequences:

>Translated_568_residues
MPNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGHIRFVPARGEAGGTNMADACARTTGGLGVCL
TSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSLAYIHEAPDQLTMLKAVSKAAFRVRSADTAISTIKLAVQTA
LTAPTGPVSVEIPIDIQAALIDMPVDLKPLPVPQHQPSAHAIDALAERLANAKRPMLWLGGGARHASKQVKRLLDLGFGV
VTSTQGRGIVAEDDPRSLGAYNLHKPVEQFYQSCDAMLVVGSRLRGNETLKYELKLPRPLLRIDADPASEGRCYQSDYFV
SGDAALALDALADRLEGRMQIDPTFADDLRRAHDTAVNALIDGLGPYSALVQALQQAVGRNFNWVRDVTVSNSTWGNRHL
QIFDSRAGVHALGGGIGQGLAMGIGAAVGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
IQDAQYGGRRHYVDLHTPDYAQLCQSLSLRHRRVSSLDDIGAALQEATAETGPFLLEIDMLSIGSFKTVFAGPPVNKQAP
IAAPATTA
>Mature_567_residues
PNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGHIRFVPARGEAGGTNMADACARTTGGLGVCLT
STGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSLAYIHEAPDQLTMLKAVSKAAFRVRSADTAISTIKLAVQTAL
TAPTGPVSVEIPIDIQAALIDMPVDLKPLPVPQHQPSAHAIDALAERLANAKRPMLWLGGGARHASKQVKRLLDLGFGVV
TSTQGRGIVAEDDPRSLGAYNLHKPVEQFYQSCDAMLVVGSRLRGNETLKYELKLPRPLLRIDADPASEGRCYQSDYFVS
GDAALALDALADRLEGRMQIDPTFADDLRRAHDTAVNALIDGLGPYSALVQALQQAVGRNFNWVRDVTVSNSTWGNRHLQ
IFDSRAGVHALGGGIGQGLAMGIGAAVGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKNI
QDAQYGGRRHYVDLHTPDYAQLCQSLSLRHRRVSSLDDIGAALQEATAETGPFLLEIDMLSIGSFKTVFAGPPVNKQAPI
AAPATTA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=543, Percent_Identity=23.9410681399632, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI93004078, Length=493, Percent_Identity=23.1237322515213, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1790104, Length=538, Percent_Identity=26.2081784386617, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=26.5917602996255, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI87081685, Length=532, Percent_Identity=25.187969924812, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787096, Length=539, Percent_Identity=23.5621521335807, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=22.8007181328546, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=23.7924865831843, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=23.7924865831843, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=25.0437828371278, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321524, Length=527, Percent_Identity=23.5294117647059, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=531, Percent_Identity=22.7871939736347, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60104; Mature: 59973

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGHIRFVPARGEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCC
GGTNMADACARTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSL
CCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AYIHEAPDQLTMLKAVSKAAFRVRSADTAISTIKLAVQTALTAPTGPVSVEIPIDIQAAL
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEE
IDMPVDLKPLPVPQHQPSAHAIDALAERLANAKRPMLWLGGGARHASKQVKRLLDLGFGV
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCE
VTSTQGRGIVAEDDPRSLGAYNLHKPVEQFYQSCDAMLVVGSRLRGNETLKYELKLPRPL
EECCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCE
LRIDADPASEGRCYQSDYFVSGDAALALDALADRLEGRMQIDPTFADDLRRAHDTAVNAL
EEECCCCCCCCCEECCCEEECCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHH
IDGLGPYSALVQALQQAVGRNFNWVRDVTVSNSTWGNRHLQIFDSRAGVHALGGGIGQGL
HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHCCCHHHHH
AMGIGAAVGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCEEECC
IQDAQYGGRRHYVDLHTPDYAQLCQSLSLRHRRVSSLDDIGAALQEATAETGPFLLEIDM
CCHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
LSIGSFKTVFAGPPVNKQAPIAAPATTA
EECCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNQQQYEQQQVTVGCAITAFLEQCGVKAAFGVISIHNMPILDAMGERGHIRFVPARGEA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHCCCCCCEEEEECCCCC
GGTNMADACARTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPMLHITGQIETPYLDQSL
CCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHH
AYIHEAPDQLTMLKAVSKAAFRVRSADTAISTIKLAVQTALTAPTGPVSVEIPIDIQAAL
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEE
IDMPVDLKPLPVPQHQPSAHAIDALAERLANAKRPMLWLGGGARHASKQVKRLLDLGFGV
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCE
VTSTQGRGIVAEDDPRSLGAYNLHKPVEQFYQSCDAMLVVGSRLRGNETLKYELKLPRPL
EECCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCE
LRIDADPASEGRCYQSDYFVSGDAALALDALADRLEGRMQIDPTFADDLRRAHDTAVNAL
EEECCCCCCCCCEECCCEEECCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHH
IDGLGPYSALVQALQQAVGRNFNWVRDVTVSNSTWGNRHLQIFDSRAGVHALGGGIGQGL
HHCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHCCCHHHHH
AMGIGAAVGAAATASGKKTFTLAGDGGFILNLGELATAVQERADMVIVLMNDKGYGVIKN
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCEEECC
IQDAQYGGRRHYVDLHTPDYAQLCQSLSLRHRRVSSLDDIGAALQEATAETGPFLLEIDM
CCHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
LSIGSFKTVFAGPPVNKQAPIAAPATTA
EECCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]