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The map label for this gene is flgJ [H]

Identifier: 71910984

GI number: 71910984

Start: 1147660

End: 1148865

Strand: Reverse

Name: flgJ [H]

Synonym: M5005_Spy_1171

Alternate gene names: 71910984

Gene position: 1148865-1147660 (Counterclockwise)

Preceding gene: 71910985

Following gene: 71910981

Centisome position: 62.49

GC content: 43.95

Gene sequence:

>1206_bases
ATGACCTTTTTAGATAAAATTAAACAAGGCTGTTTAGATGGCTGGACAACGTACAAAATCTTGCCATCCTTGACCGCAGC
GCAGACAATCTTAGAAAGCGGGTGGGGCAAACATGCCCCACACAACGCTCTGTTTGGTATTAAGGCAGATAGCTCTTGGA
CTGGTAAATCATTTGATACCAAAACCCAAGAGGAATATCAAGCAGGTGTTGTCACGGATATTGTGGACCGATTTAGGGCG
TATGATAGTTGGACTGACAGCATTATTGATCACGGTAAATTTTTAAACGATAATCCACGGTATCAGGCTGTGATTGGTGA
GACTGATTACAAAAAAGCTTGTCACGCTATAAAAGATGCAGGTTATGCCACAGCTAGCGGTTATGCAGAGTTGCTTATCC
AACTAATCGAGGAAAATGACCTTCAGGAATGGGATGAAGAGGCTATTGTCAGAAAGGAGAAGCAAATGATTAGTTCTCAA
TGTCGAGAAGTCATTGAATTTTTTATTAACTTGGTAAATGCTGGGGTTGGCGTGGATAAAGATGGTTTTGCGGGCTGGCA
ATGCACAGATGTGCCTTGTTATGCAGCAAAGCACTGGTTTGGTGTTGATCTTTGGGGAAATGCGATTGACTTACTAGATA
GCGCTGCTGCCGTAGGTTGGGAAGTCCACCGCATGCCGACAGATGCAAATCCACAGACTGGAGCATTTTTTGTCCAATCA
GTGCCATATCATCAATTTGGTCACACAGGAGTTGTCATTGAGGATAGCGACGGTTATACCATGCGAACTATCGAGCAAAA
CATTGATGGCAATCCTGATGCTTTGTATGTCGGTGCACCAGCTCGTTTTAATACTCGTGATTTTACCGGCGTGACAGGAT
GGTTTTACCCTCCTTACCAAGGAGATATTGTCACGCAACCAGTCAGCACCGAGCCACAAACGTCTGACACTATCGTAGAG
ACAGCAAAAACAGGTACTTTTACGCTTGATGTCGCAGAAATCAATATCAGACGTTGGCCAAGTTTAACTAGCGAGGTAGT
AGGTAGCTATAAACAAGGTGATACTATCAGCTTTGATAGTGAGGGTTACGCCAATGGTTACTACTGGATTAGCTACGTTG
GCGGCTCGGGTATGCGAAACTACACGGCTATTGGGATAACTGACAAAGACGGTAACATTATCAGCCTTTGGGGCAAATTA
AATTAG

Upstream 100 bases:

>100_bases
CAATCACACGATTAGCATTAGTTATAGCAATCGCAATACTGTATGTGCCGATATCTGTTGTTGCTCTGATCTTTTATCCA
TTTTTAGATAAGGAGGACAG

Downstream 100 bases:

>100_bases
GAGATAAAGCTCCTTTAGATAAGACAAAACCGCTCTCTTAATCGAGGGCGGTTTTTTGTGTTTAAGTAATTATTTTTTTA
ATTTTTTTCTTTCATTTTCA

Product: phage-associated cell wall hydrolase

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTFLDKIKQGCLDGWTTYKILPSLTAAQTILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRA
YDSWTDSIIDHGKFLNDNPRYQAVIGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQEWDEEAIVRKEKQMISSQ
CREVIEFFINLVNAGVGVDKDGFAGWQCTDVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPQTGAFFVQS
VPYHQFGHTGVVIEDSDGYTMRTIEQNIDGNPDALYVGAPARFNTRDFTGVTGWFYPPYQGDIVTQPVSTEPQTSDTIVE
TAKTGTFTLDVAEINIRRWPSLTSEVVGSYKQGDTISFDSEGYANGYYWISYVGGSGMRNYTAIGITDKDGNIISLWGKL
N

Sequences:

>Translated_401_residues
MTFLDKIKQGCLDGWTTYKILPSLTAAQTILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRA
YDSWTDSIIDHGKFLNDNPRYQAVIGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQEWDEEAIVRKEKQMISSQ
CREVIEFFINLVNAGVGVDKDGFAGWQCTDVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPQTGAFFVQS
VPYHQFGHTGVVIEDSDGYTMRTIEQNIDGNPDALYVGAPARFNTRDFTGVTGWFYPPYQGDIVTQPVSTEPQTSDTIVE
TAKTGTFTLDVAEINIRRWPSLTSEVVGSYKQGDTISFDSEGYANGYYWISYVGGSGMRNYTAIGITDKDGNIISLWGKL
N
>Mature_400_residues
TFLDKIKQGCLDGWTTYKILPSLTAAQTILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAY
DSWTDSIIDHGKFLNDNPRYQAVIGETDYKKACHAIKDAGYATASGYAELLIQLIEENDLQEWDEEAIVRKEKQMISSQC
REVIEFFINLVNAGVGVDKDGFAGWQCTDVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPQTGAFFVQSV
PYHQFGHTGVVIEDSDGYTMRTIEQNIDGNPDALYVGAPARFNTRDFTGVTGWFYPPYQGDIVTQPVSTEPQTSDTIVET
AKTGTFTLDVAEINIRRWPSLTSEVVGSYKQGDTISFDSEGYANGYYWISYVGGSGMRNYTAIGITDKDGNIISLWGKLN

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=122, Percent_Identity=36.0655737704918, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44525; Mature: 44394

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLDKIKQGCLDGWTTYKILPSLTAAQTILESGWGKHAPHNALFGIKADSSWTGKSFDT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQAGVVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYQAVIGETDYKKACHAIKDA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQLIEENDLQEWDEEAIVRKEKQMISSQCREVIEFFINLVNAGVGVDK
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGFAGWQCTDVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPQTGAFFVQS
CCCCCCEECCCCHHHHHHEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEC
VPYHQFGHTGVVIEDSDGYTMRTIEQNIDGNPDALYVGAPARFNTRDFTGVTGWFYPPYQ
CCCHHCCCEEEEEECCCCCEEEEEHHCCCCCCCEEEECCCCCCCCCCCCCCCCEECCCCC
GDIVTQPVSTEPQTSDTIVETAKTGTFTLDVAEINIRRWPSLTSEVVGSYKQGDTISFDS
CCEEECCCCCCCCCCHHHHHHCCCCEEEEEEEEEEEHHCCCHHHHHHCCCCCCCEEEECC
EGYANGYYWISYVGGSGMRNYTAIGITDKDGNIISLWGKLN
CCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEEEEECCC
>Mature Secondary Structure 
TFLDKIKQGCLDGWTTYKILPSLTAAQTILESGWGKHAPHNALFGIKADSSWTGKSFDT
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQAGVVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYQAVIGETDYKKACHAIKDA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQLIEENDLQEWDEEAIVRKEKQMISSQCREVIEFFINLVNAGVGVDK
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGFAGWQCTDVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPQTGAFFVQS
CCCCCCEECCCCHHHHHHEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEC
VPYHQFGHTGVVIEDSDGYTMRTIEQNIDGNPDALYVGAPARFNTRDFTGVTGWFYPPYQ
CCCHHCCCEEEEEECCCCCEEEEEHHCCCCCCCEEEECCCCCCCCCCCCCCCCEECCCCC
GDIVTQPVSTEPQTSDTIVETAKTGTFTLDVAEINIRRWPSLTSEVVGSYKQGDTISFDS
CCEEECCCCCCCCCCHHHHHHCCCCEEEEEEEEEEEHHCCCHHHHHHCCCCCCCEEEECC
EGYANGYYWISYVGGSGMRNYTAIGITDKDGNIISLWGKLN
CCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]