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The map label for this gene is pucK [H]

Identifier: 71910672

GI number: 71910672

Start: 849906

End: 851189

Strand: Direct

Name: pucK [H]

Synonym: M5005_Spy_0859

Alternate gene names: 71910672

Gene position: 849906-851189 (Clockwise)

Preceding gene: 71910671

Following gene: 71910675

Centisome position: 46.23

GC content: 40.73

Gene sequence:

>1284_bases
ATGACACATTCAACAAAGCAGGAACATTCTCACTCGCAATCAGCTGTCCTTGGCTTACAGCATGTGCTTTCGATGTATGC
CGGATCTATTTTAGTACCAATTATGATCGCAGGTGCTTTAGGTTATTCCGCTAGAGAGTTGACCTACCTGATTTCGACAG
ATATTTTCATGTGTGGTGTGGCTACTTTCTTACAGTTAAAGTTAACCAAGCATACGGGAGTGGGATTACCAGTTGTTTTA
GGATGTGCTTTTCAATCAGTGGCACCTCTATCCATTATTGGAGCGCAACAAGGTTCAGGTGCCAAGTTTGGAGCCCTCAT
TGCGTCAGGAATTTATGTCATTTTAGTGGCAGGTATTTTTTCTAAGATTGCTCGTTTCTTCCCACCAATTGTTACAGGAT
CGGTCATTACCGTTATTGGCTTAAGTCTCGTAGGTGTTGCTATGGGGAATATGGGAGATAACGTCAAAGAGCCTACGGCA
CAAAGCATGATGTTATCATTGTTGACGATTGTCATTATTCTTTTAGTCCAAAAATTTACCAAAGGTTTTGTGAAATCCAT
TTCCATTTTGATTGGGCTTGTAGCGGGAACTTTGGTTTCAGCTATGATGGGATTAGTAGATACAACTCCTGTTGTAGAGG
CTTCTTGGATTCATGTGCCAACCCCATTTTATTTTGGCATGCCAACTTTTGAAATCACTTCTATTGTGATGATGTGTATC
ATCGCAACGGTTTCTATGGTTGAATCAACAGGTGTCTACTTAGCGCTTTCAGATTTGACTAATGATCAATTAGATGAAAA
ACGTTTGCGCAATGGTTATCGCTCAGAAGGGATCGCAGTCTTTCTCGGTGGGTTATTTAACACCTTCCCTTACACTGGTT
TTTCTCAAAACGTTGGACTTGTCCAAATCTCAGGTATTAAAACCCGTCGTCCGATTTACTACGCAGCAGGTATCCTTGTG
GTCATTGGCCTACTCCCTAAATTTGGAGCAATGGCACAAATGATTCCAAGCCCAGTTCTTGGTGGAGCCATGCTTGTTCT
CTTTGGTATGGTTGCTCTTCAAGGAATGCAAATGCTTAATCGTGTTGATTTCCAAAAGAACGAGTATAATTTTATTATTG
CTGCCGTTTCCATCTCAGCAGGTTTAGGCTTTAATGGCACTAATCTTTTTGCCAGCTTACCAGAGACAGCTCAAATGTTC
TTAACGAATGGTATTGTGATTGCCACTTTGACCTCTGTTGTCTTAAATTTAGTTTTAAATGGCAAAGACAAACAAGATGA
ATAA

Upstream 100 bases:

>100_bases
GTCGTCGATTGATTGAAGATATGGGCATTGAAGTGACCTCATTAGCCCGTATTAAAAACTTTGAAAATGGTAATCTAAAC
TTCTTGGAGGCTGACGCATA

Downstream 100 bases:

>100_bases
TTAAAAAACGGCTAATTGAGCCGTTTTTTCTGTGATCAGTTTTGAGCAAACCGTTAACCCCTTTTAATGATAGTATGGTT
GAAAATAGTGTCTCAAACCA

Product: xanthine permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVL
GCAFQSVAPLSIIGAQQGSGAKFGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFEITSIVMMCI
IATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILV
VIGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMF
LTNGIVIATLTSVVLNLVLNGKDKQDE

Sequences:

>Translated_427_residues
MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVL
GCAFQSVAPLSIIGAQQGSGAKFGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFEITSIVMMCI
IATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILV
VIGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMF
LTNGIVIATLTSVVLNLVLNGKDKQDE
>Mature_426_residues
THSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLG
CAFQSVAPLSIIGAQQGSGAKFGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTAQ
SMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFEITSIVMMCII
ATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVV
IGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMFL
TNGIVIATLTSVVLNLVLNGKDKQDE

Specific function: Uptake of uric acid [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=437, Percent_Identity=25.629290617849, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI40316845, Length=437, Percent_Identity=25.629290617849, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI44680145, Length=422, Percent_Identity=25.5924170616114, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI44680143, Length=426, Percent_Identity=25.3521126760563, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI87082181, Length=436, Percent_Identity=38.302752293578, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790087, Length=447, Percent_Identity=29.5302013422819, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788843, Length=419, Percent_Identity=28.6396181384248, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87081818, Length=439, Percent_Identity=28.0182232346241, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082178, Length=435, Percent_Identity=26.4367816091954, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17541904, Length=420, Percent_Identity=22.1428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558856, Length=155, Percent_Identity=27.741935483871, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17542262, Length=192, Percent_Identity=27.6041666666667, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356175, Length=424, Percent_Identity=25.9433962264151, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45312; Mature: 45180

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCC
>Mature Secondary Structure 
THSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGV
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAKFGALIASGIYVILVAGIF
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
SKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTAQSMMLSLLTIVIILLVQKFT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFEITSIVMMCI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECEEECCCCHHCCCCHHHHHHHHHHHH
IATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGL
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCE
VQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQMIPSPVLGGAMLVLFGMVALQGMQMLN
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RVDFQKNEYNFIIAAVSISAGLGFNGTNLFASLPETAQMFLTNGIVIATLTSVVLNLVLN
HHCCCCCCCEEEEEEEEEECCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHC
GKDKQDE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]