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The map label for this gene is dpiB [H]

Identifier: 71910644

GI number: 71910644

Start: 824227

End: 825768

Strand: Reverse

Name: dpiB [H]

Synonym: M5005_Spy_0831

Alternate gene names: 71910644

Gene position: 825768-824227 (Counterclockwise)

Preceding gene: 71910647

Following gene: 71910643

Centisome position: 44.91

GC content: 39.3

Gene sequence:

>1542_bases
ATGAAAAAACCACTACGTTTATGGGCCAGTTTATCGTTGATTTTGGTATCTATGATTGTTGTCACAACCTCTCTATTTTA
CGGGATTATGCTACATGATACGCACCAATCCATCAAAAATCAGGAAACCCATTTGCTAACGTCAACTGGAAAAATGTTAG
CAAGTCATCAAGCGATTAAAGAATTACTCCTAAACAACCAACCAAATGCTAAAACAACAGCCTATACCAACTCCATTGCC
TCCATCTATAACCTTGATTATGTGGTCGTGATGAACATGAAAGGGATTCGACTCACCCATCCTAACCCGAAAAATATCGG
AAAACCTTTTCAAGGAGGTGATGAGGAGGCTGTTCTAGCAGGAAAAAAGGTTATCTCTACCGCCAAGGGCACTTTAGGAA
AGTCACTTCGCTATCTAGTACCTGTGTTTGACGGTGACAAACAGATTGGTGCTATTGCGGTTGGCATTAAACTAACTACC
CTAAATGATGTGGCTTTAACGTCAAAACGCAATTATACCCTGTCTTTACTCCTTTGTCTTCTTATTAGTTTACTGGTCAC
CAGTTTTATTTCTTTTCGACTCAAGCGTCAACTGCACCAGTTAGAACCAAGTGAAATTTATCAGCTCTTTGAAGAGCGTA
ATGCCATGTTGGATCAAATCGAAGCCGCCGTATTTGTGGTAGATAAAGCTGGTATCTTACAGCTCTGCAATCAGGCTGGA
CAAAAATTAATTGCTCGGAAATGCCAGTTGGGAAAACCTACTGGCAACAGTTTCAATTACCTCTTTCCTGATTTTCCAAA
ACTTAGTTTGCAAGAGGGGCATGAACAACTCTTTCGGTATGGTGAGGAAGACTACTTGTTAGCCATTTCACCCATCTGTG
TCAAAAACGATCACCGAGGCCATATTATTTTCATGCGAGAAGCAGTTAAGGCTATTGATACCTTGGATCAGCTAGCCTAT
ACCACTGCCTATGCTTCTGCTCTACAAGCCCAAACTCATAAATTCATGAATCAATTGCATGTTATTTATGGCTTAGTGGA
TATTGCTTATTATGACCAATTGAAAATTTATTTAGACAGTATTTTGGAACCCGAAAATGAAATTCTCACTAGCTTATCTG
TCCTTGTTAAGGAGCCTCTTCTAGCTAGTTTTCTCATTGGAGAGCAAGAGAAATACCAAGAATTAAATGTCCATCTCAAG
ATTGATGTTTTAAGCGAGATTCCTCATTCTGCCACCAAGAATCAACTTAATAATGGTTTGATGATTTACCGCTTTATCCA
TACTAATCTTCTAACCACACTGAGACCCAAATCACTCGTTTTGAGTATTCAACATGACCAGAATCATTTAATCAGCCATT
ACACCTTGACAGACAATTGGATTGATTTGGAGCGGGTACAGCCTATTTTTGACTTACCTTATTTTCAACAGCTCTTGACC
GATACAAATTCCCAATTCCATCAACATCAAACCGTAACCGAGTTACAGTTCTCAGTGACAACACCTTATGTAGGAGGGCA
ACATGAACGTTTTAATCATTGA

Upstream 100 bases:

>100_bases
ATTTAAGTTGTTTAACTACTTTAATTTTTTCAAGTTATTTAATTAATTTAAGCTAGCAGGTCATTTCTTATCGTGTTATA
CTCAATAAAGAGGAGACCTT

Downstream 100 bases:

>100_bases
AGATGATCCTATGGTGGATTTTATCCATCGCAATTACCTCGAAAAACTAAATCTTTTTGATCGCATTATATCCAGCGACT
CTATGAAAGCTGTTCAAAGC

Product: sensor kinase

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETHLLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIA
SIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAG
QKLIARKCQLGKPTGNSFNYLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAY
TTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLK
IDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLPYFQQLLT
DTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH

Sequences:

>Translated_513_residues
MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETHLLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIA
SIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAG
QKLIARKCQLGKPTGNSFNYLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAY
TTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLK
IDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLPYFQQLLT
DTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH
>Mature_513_residues
MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETHLLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIA
SIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAG
QKLIARKCQLGKPTGNSFNYLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAY
TTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLK
IDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLPYFQQLLT
DTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=449, Percent_Identity=27.8396436525612, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1786837, Length=468, Percent_Identity=22.4358974358974, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58158; Mature: 58158

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETHLLTSTGKMLASHQAIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHCCCCCEEEEHHHHHHHHHHHHH
ELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLA
HHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCHHCCCCCCCCCCHHHHH
GKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTTLNDVALTSKRNYTLSLLLCL
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEEEECCEEECCCCCHHHHHHHHH
LISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHH
QKLIARKCQLGKPTGNSFNYLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHRG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEECCCCC
HIIFMREAVKAIDTLDQLAYTTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIYLDS
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLKIDVLSEIPHSATKNQLNNGL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEEEHHHCCCHHHHHHCCCCE
MIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLPYFQQLLT
EEEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCEEHHHCCHHHCCHHHHHHHH
DTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH
CCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETHLLTSTGKMLASHQAIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHCCCCCEEEEHHHHHHHHHHHHH
ELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLA
HHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCHHCCCCCCCCCCHHHHH
GKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTTLNDVALTSKRNYTLSLLLCL
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEEEEECCEEECCCCCHHHHHHHHH
LISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHH
QKLIARKCQLGKPTGNSFNYLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHRG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEECCCCC
HIIFMREAVKAIDTLDQLAYTTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIYLDS
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLKIDVLSEIPHSATKNQLNNGL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEEEHHHCCCHHHHHHCCCCE
MIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLPYFQQLLT
EEEEEHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCEEHHHCCHHHCCHHHHHHHH
DTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH
CCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]